6 results match your criteria: "Center of Excellence in Genomics (CEG)[Affiliation]"

Objective: In peanut, the DNA polymorphism is very low despite enormous phenotypic variations. This limits the use of genomics-assisted breeding to enhance peanut productivity. This study aimed to develop and validate new AhMITE1 and cleaved amplified polymorphic sequences (CAPS) markers.

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Identification and Validation of Selected Universal Stress Protein Domain Containing Drought-Responsive Genes in Pigeonpea (Cajanus cajan L.).

Front Plant Sci

January 2016

Center of Excellence in Genomics (CEG), International Crops Research Institute for the Semi-Arid Tropics (ICRISAT)Hyderabad, India; School of Plant Biology and the Institute of Agriculture, The University of Western AustraliaPerth, WA, Australia.

Pigeonpea is a resilient crop, which is relatively more drought tolerant than many other legume crops. To understand the molecular mechanisms of this unique feature of pigeonpea, 51 genes were selected using the Hidden Markov Models (HMM) those codes for proteins having close similarity to universal stress protein domain. Validation of these genes was conducted on three pigeonpea genotypes (ICPL 151, ICPL 8755, and ICPL 227) having different levels of drought tolerance.

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A combination of two approaches, namely QTL analysis and gene enrichment analysis were used to identify candidate genes in the "QTL-hotspot" region for drought tolerance present on the Ca4 pseudomolecule in chickpea. In the first approach, a high-density bin map was developed using 53,223 single nucleotide polymorphisms (SNPs) identified in the recombinant inbred line (RIL) population of ICC 4958 (drought tolerant) and ICC 1882 (drought sensitive) cross. QTL analysis using recombination bins as markers along with the phenotyping data for 17 drought tolerance related traits obtained over 1-5 seasons and 1-5 locations split the "QTL-hotspot" region into two subregions namely "QTL-hotspot_a" (15 genes) and "QTL-hotspot_b" (11 genes).

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Article Synopsis
  • Over 2000 single-nucleotide polymorphisms (SNPs) were identified in pigeonpea using RNA-seq techniques, leading to the development of 1616 cost-effective genotyping assays called PKAMs (pigeonpea KASPar assay markers).
  • A screening of these PKAMs in 24 pigeonpea genotypes revealed 1154 polymorphic markers, which were used to create a detailed genetic map from 167 F(2) lines, incorporating both new and previously mapped markers for a total length of 996.21 cM.
  • The mapped PKAMs highlighted significant genetic similarities with the soybean genome and can facilitate future genetics research and breeding strategies in pigeonpea and related legume species.
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