A colorimetric and SERS-based LFIA for sensitive and simultaneous detection of three stroke biomarkers: An ultra-fast and sensitive point-of-care testing platform.

Stroke ranks as the second leading cause of disability and mortality globally. Biomarker detection represents a promising avenue for predicting disease severity and prognosis. The expression levels of metalloproteinase-9 (MMP-9), neuron-specific enolase (NSE), and N-terminal pro-brain natriuretic peptide (NT-pro BNP) in blood correlate with stroke severity.

Multicolor Fluorescent Inks Based on Lanthanide Hybrid Organogels for Anticounterfeiting and Logic Circuit Design.

With the rapid development of information technology, the encrypted storage of information is becoming increasingly important for human life. The luminescent materials with a color-changed response under physical or chemical stimuli are crucial for information coding and anticounterfeiting. However, traditional fluorescent materials usually face problems such as a lack of tunable fluorescence, insufficient surface-adaptive adhesion, and strict synthesis conditions, hindering their practical applications.

To What Extent Do Low-Voltage Electrostatic Fields Play a Role in the Physicochemical Properties of Pork during Freezing and Storage?

Low-voltage electrostatic fields (LVEF) are recognized as a new technology that can improve the quality of frozen meat. To determine the extent to which LVEF assistance affects the quality of frozen pork for long-term storage, pork was frozen and stored at -18 and -38 °C for up to 5 months. Water-holding capacity, muscle microstructure, and protein properties were investigated after up to 5 months of frozen storage with and without LVEF assistance.

Comparative study of three cultivars of jaboticaba berry: nutrient, antioxidant and volatile compounds.

Jaboticaba is a tropical plant and its fruit rich in nutrients, volatile compounds, and biological activities, which considered to be an edible health benefits plant. Despite its popularity for fresh consumption, jaboticaba is rarely used in intensive processing in China. The content of nutrients and antioxidant in jaboticaba greatly impacts how it is processed healthy food.

PathwayKO: An integrated platform for deciphering the systems-level signaling pathways.

Systems characterization of immune landscapes in health, disease and clinical intervention cases is a priority in modern medicine. High-throughput transcriptomes accumulated from gene-knockout (KO) experiments are crucial for deciphering target KO signaling pathways that are impaired by KO genes at the systems-level. There is a demand for integrative platforms.

Chemical speciation and bioavailability of potentially toxic elements in surface sediment from the Huaihe River, Anhui Province, China.

In order to understand the characteristics of speciation and ecological risk of potentially toxic element (PTE) pollution in the surface sediment of huaihe river (Anhui province), 23 surface sediment samples were collected. The occurrence characteristics of PTEs (As, Cr, Zn, Cu, Cd, Pb, Mn) were analyzed by modified continuous extraction method (BCR), and the pollution status and potential ecological risk of PTEs were comprehensively evaluated by Pollution Load Index (PLI), Geoaccumulation Index (Igeo), Enrichment Factor (EF) and the risk assessment code (RAC). Results showed that the total concentrations of As, Mn, Cd, Cr, Cu, Pb, and Zn in sediment were 14.

Recent Advances in g-CN-Based Materials and Their Application in Energy and Environmental Sustainability.

Graphitic carbon nitride (g-CN), with facile synthesis, unique structure, high stability, and low cost, has been the hotspot in the field of photocatalysis. However, the photocatalytic performance of g-CN is still unsatisfactory due to insufficient capture of visible light, low surface area, poor electronic conductivity, and fast recombination of photogenerated electron-hole pairs. Thus, different modification strategies have been developed to improve its performance.

CASP-Model Sepsis Triggers Systemic Innate Immune Responses Revealed by the Systems-Level Signaling Pathways.

Colon ascendens stent peritonitis (CASP) surgery induces a leakage of intestinal contents which may cause polymicrobial sepsis related to post-operative failure of remote multi-organs (including kidney, liver, lung and heart) and possible death from systemic syndromes. Mechanisms underlying such phenomena remain unclear. This article aims to elucidate the mechanisms underlying the CASP-model sepsis by analyzing real-world GEO data (GSE24327_A, B and C) generated from mice spleen 12 hours after a CASP-surgery in septic MyD88-deficient and wildtype mice, compared with untreated wildtype mice.

MIMRDA: A Method Incorporating the miRNA and mRNA Expression Profiles for Predicting miRNA-Disease Associations to Identify Key miRNAs (microRNAs).

Identifying cancer-related miRNAs (or microRNAs) that precisely target mRNAs is important for diagnosis and treatment of cancer. Creating novel methods to identify candidate miRNAs becomes an imminent Frontier of researches in the field. One major obstacle lies in the integration of the state-of-the-art databases.

Monitoring and dietary exposure assessment of pesticide residues in strawberry in Beijing, China.

In this study, 142 pesticide residues in 245 strawberry samples, gathered from farmers markets or supermarkets in Beijing from June 2017 to May 2018, were investigated. The samples were analysed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). According to the validation data, including linearity, recovery, precision and measurement uncertainty, the method performed well in monitoring pesticide residues and complied with the requirements of the European Guideline SANTE/11813/2017.

A preliminary study on pollution characteristics of surfactant substances in fine particles in the Beibu Gulf Region of China.

The pollution characteristics of surfactant substances in fine particles (PM) in spring were studied in the Beibu Gulf Region of China, 68 samples of PM were collected at Weizhou Island in Beihai City from March 12 to April 17, 2015. The Anionic Surfactant Substances (ASS) and Cationic Surfactant Substances (CSS) in the samples were analyzed using Byethyl Violet Spectrophotometry and Disulfide Blue Spectrophotometry, respectively. Combined with the data from backward trajectory simulation, the effects of air pollutants from remote transport on the pollution characteristics of surfactant substances in PM in the Beibu Gulf Region were analyzed and discussed.

Integrated transcriptomic and proteomic analyses of the tissues from the digestive gland of Chlamys farreri following cadmium exposure.

Objectives: Cadmium causes the pollution of marine habitat and Chlamys farreri is an effective concentrator of heavy metals, the aim of this study was to study the response mechanism of C. farreri to cadmium stress at transcriptomic and proteomic levels.

Methods: Transcriptomic analysis based on RNA-sequencing and proteomic analysis based on isobaric tags for relative and absolute quantitation were performed to reveal the molecular response of C.

[Simultaneous determination of 12 preservatives and antioxidants in fruit and vegetable detergents by gas chromatography-mass spectrometry].

A method was established for the simultaneous determination of 12 preservatives and antioxidants in fruit and vegetable detergents by gas chromatography-mass spectrometry (GC-MS). First, ethanol was added to remove the water in the sample by rotary evaporation and was extracted with acetonitrile in saturated -hexane. Finally, the residual surfactant in the sample solution was removed with a saturated sodium chloride solution.

CuO/hollow mesoporous silica composites for the rapid and efficient removal of methylene blue.

CuO/hollow mesoporous silica (HMS) composite was synthesized using HMS as supporting material by the impregnation method. This composite displayed integrated physicochemical performance of CuO and HMS, resulting in low density, large surface area and excellent dispersibility. The synthesized nano-sized composite of CuO/HMS demonstrated rapid and effective removal for methylene blue with an efficiency of 99.

[Determination of fipronil and its metabolites in feeds by stable isotope dilution ultra high performance liquid chromatography-tandem mass spectrometry].

A method based on ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was established for the determination of fipronil and its metabolites in feeds. Sodium chloride was added to the samples after the ultrasonic extraction of pure water and acetonitrile, and then cleaned up by the QuEChERS method. The target analytes were separated on a Waters HSS T3 C column using methanol and water as mobile phases with gradient elution, and detected by a MS/MS system under negative electro-spry ionization (ESI) and the multiple reaction monitoring (MRM) mode.

Extreme learning machine: a new alternative for measuring heat collection rate and heat loss coefficient of water-in-glass evacuated tube solar water heaters.

Background: Heat collection rate and heat loss coefficient are crucial indicators for the evaluation of in service water-in-glass evacuated tube solar water heaters. However, the direct determination requires complex detection devices and a series of standard experiments, wasting too much time and manpower.

Findings: To address this problem, we previously used artificial neural networks and support vector machine to develop precise knowledge-based models for predicting the heat collection rates and heat loss coefficients of water-in-glass evacuated tube solar water heaters, setting the properties measured by "portable test instruments" as the independent variables.

Artificial Neural Networks-Based Software for Measuring Heat Collection Rate and Heat Loss Coefficient of Water-in-Glass Evacuated Tube Solar Water Heaters.

Measurements of heat collection rate and heat loss coefficient are crucial for the evaluation of in service water-in-glass evacuated tube solar water heaters. However, conventional measurement requires expensive detection devices and undergoes a series of complicated procedures. To simplify the measurement and reduce the cost, software based on artificial neural networks for measuring heat collection rate and heat loss coefficient of water-in-glass evacuated tube solar water heaters was developed.

Distribution of Marine Lipophilic Toxins in Shellfish Products Collected from the Chinese Market.

To investigate the prevalence of lipophilic marine biotoxins in shellfish from the Chinese market, we used hydrophilic interaction liquid chromatography-tandem mass spectrometry (LC-MS/MS) to measure levels of okadaic acid (OA), azaspiracid (AZA1), pectenotoxin (PTX2), gymnodimine (GYM), and spirolide (SPX1). We collected and analyzed 291 shellfish samples from main production sites along a wide latitudinal transect along the Chinese coastline from December 2008 to December 2009. Results revealed a patchy distribution of the five toxins and highlighted the specific geographical distribution and seasonal and species variation of the putative toxigenic organisms.

Discrimination of producing area of Chinese Tongshan kaoliang spirit using electronic nose sensing characteristics combined with the chemometrics methods.

In the ancient history of the Yue Nation, the Chinese Tongshan kaoliang spirit (CTKS) has been one of the most popular liquor in the last 2,500 years. The most common fraudulent practice for the commercialization of CTKS is to produce and sell adulterated spirit from different geographical origins. In this study, the use of GC-flash electronic nose (EN) technique combined with chemometrics analysis has proven to provide a rapid tool for the discrimination of CTKS from different geographical origins.

Liquid chromatography quadrupole linear ion trap mass spectrometry for multiclass screening and identification of lipophilic marine biotoxins in bivalve mollusks.

A liquid chromatography quadrupole linear ion trap mass spectrometry method with fast polarity switching and a scheduled multiple reaction monitoring algorithm mode was developed for multiclass screening and identification of lipophilic marine biotoxins in bivalve molluscs. A major advantage of the method is that it can detect members of all six groups of lipophilic marine biotoxins [okadaic acid (OA), yessotoxins (YTX), azaspiracids (AZA), pectenotoxins (PTX), cyclic imines (CI), and brevetoxins (PbTx)], thereby allowing quantification and high confidence identification from a single liquid chromatography tandem mass spectrometry (LC-MS/MS) injection. An enhanced product ion (EPI) library was constructed after triggered collection of data via information-dependent acquisition (IDA) of EPI spectra from standard samples.

Simultaneous determination of 14 oil-soluble synthetic dyes in chilli products by high performance liquid chromatography with a gel permeation chromatography clean-up procedure.

A method was developed for the simultaneous determination of 14 fat-soluble dyes in chilli products. The samples were extracted with hexane/acetone. The cleanup was performed with gel permeation chromatography (GPC) cleanup system.

[Simultaneous determination of 9 ultraviolet stabilizers in food plastic packaging materials by solid phase extraction-high performance liquid chromatography].

An effective high performance liquid chromatographic (HPLC) method has been developed for the simultaneous determination of 9 ultraviolet stabilizers in food plastic packaging materials. The food packaging samples were firstly extracted by methanol-ethyl acetate, and then purified by a C18 solid-phase extraction (SPE) column. The target compounds were separated on a ZORBAX SB-C18 column (250 mm x 4.

[A method for the analysis of overlapped peaks in the high performance liquid chromatogram based on spectrum analysis].

A method was established to analyse the overlapped chromatographic peaks based on the chromatographic-spectra data detected by the diode-array ultraviolet detector. In the method, the three-dimensional data were de-noised and normalized firstly; secondly the differences and clustering analysis of the spectra at different time points were calculated; then the purity of the whole chromatographic peak were analysed and the region were sought out in which the spectra of different time points were stable. The feature spectra were extracted from the spectrum-stable region as the basic foundation.

Effect of EDTA, HCl, and citric acid on Ca salt removal from Asian (silver) carp scales prior to gelatin extraction.

Pretreatments with different chemicals at different concentrations to remove Ca compounds were studied to determine their effects on gelatin extraction from silver carp (Hypophthalmichthys molitrix) scales. During Ca removal with HCl, citric acid, and EDTA, all 3 chemicals were able to decalcify (>90%) scales; however, protein losses with EDTA were lower than with HCl and citric acid (P < 0.05), and protein losses with citric acid were lower than with HCl (P < 0.

[Determination of melamine residue in foods using gas chromatography-tandem mass spectrometry].

A method of gas chromatography-tandem mass spectrometry (GC-MS/MS) was developed for melamine residue in foods. The sample was extracted under ultrasonication with trichloroacelic acid (TCA), the protein in the sample was precipitated with zinc acetate solution. The extract was centrifuged, filtered, purified, derivatized and detected using GC-MS/MS in multiple-reaction monitoring mode.