47 results match your criteria: "Center for Nanomedicine and Tissue Engineering[Affiliation]"
Unveiling Interactions between Self-Assembling Peptides and Neuronal Membranes.
Langmuir
December 2024
Center for Nanomedicine and Tissue Engineering (CNTE), A.S.S.T. Grande Ospedale Metropolitano Niguarda, Piazza dell'Ospedale Maggiore 3, Milan 20162, Italy.
The use of self-assembling peptide hydrogels in the treatment of spinal cord and brain injuries, especially when combined with adult neural stem cells, has shown great potential. To advance tissue engineering, it is essential to understand the effect of mechanochemical signaling on cellular differentiation. The elucidation of the molecular interactions at the level of the neuronal membrane still represents a promising area of investigation for many drug delivery and tissue engineering applications.
View Article and Find Full Text PDFBiomimetic electrospun PVDF/self-assembling peptide piezoelectric scaffolds for neural stem cell transplantation in neural tissue engineering.
RSC Adv
July 2024
Center for Nanomedicine and Tissue Engineering (CNTE), ASST Grande Ospedale Metropolitano Niguarda 20162 Milan Italy
Piezoelectric materials can provide electrical stimulation without external chemical or physical support, opening new frontiers for future bioelectric therapies. Polyvinylidene fluoride (PVDF) possesses piezoelectricity and biocompatibility, making it an electroactive biomaterial capable of enhancing bioactivity through instantaneous electrical stimulation, which indicates significant potential in tissue engineering. In this study, we developed electroactive and biomimetic scaffolds made of electrospun PVDF and self-assembling peptides (SAPs) to enhance stem cell transplantation for spinal cord injury regeneration.
View Article and Find Full Text PDFMultiparametric in vitro and in vivo analysis of the safety profile of self-assembling peptides.
Sci Rep
February 2024
Center for Nanomedicine and Tissue Engineering (CNTE), ASST Grande Ospedale Metropolitano Niguarda, 20162, Milan, Italy.
Self-assembling peptides (SAPs) have gained significant attention in biomedicine because of their unique properties and ability to undergo molecular self-assembly driven by non-covalent interactions. By manipulating their composition and structure, SAPs can form well-ordered nanostructures with enhanced selectivity, stability and biocompatibility. SAPs offer advantages such as high chemical and biological diversity and the potential for functionalization.
View Article and Find Full Text PDFTransglutaminase Cross-Linking Improves Mechanical Properties of Self-Assembling Peptides for Biomedical Applications.
ACS Appl Bio Mater
March 2024
Institute for Stem-Cell Biology, Regenerative Medicine and Innovative Therapies, IRCCS Casa Sollievo della Sofferenza, 71013 San Giovanni Rotondo, Italy.
The development of three-dimensional (3D) biomaterials that mimic natural tissues is required for efficiently restoring physiological functions of injured tissues and organs. In the field of soft hydrogels, self-assembled peptides (SAPs) stand out as distinctive biomimetic scaffolds, offering tunable properties. They have garnered significant attention in nanomedicine due to their innate ability to self-assemble, resulting in the creation of fibrous nanostructures that closely mimic the microenvironment of the extracellular matrix (ECM).
View Article and Find Full Text PDFEnhancing the Features of DNA Mimic Foldamers for Structural Investigations.
Chemistry
March 2024
Department of Pharmacy, Ludwig-Maximilians-Universität München, Butenandtstr. 5-13, 81377, Munich, Germany.
DNA mimic foldamers based on aromatic oligoamide helices bearing anionic phosphonate side chains have been shown to bind to DNA-binding proteins sometimes orders of magnitude better than DNA itself. Here, we introduce new features in the DNA mimic foldamers to facilitate structural investigations of their interactions with proteins. Thirteen new foldamer sequences have been synthesized and characterized using NMR, circular dichroism, molecular modeling, and X-ray crystallography.
View Article and Find Full Text PDFBiomimetic Electrospun Self-Assembling Peptide Scaffolds for Neural Stem Cell Transplantation in Neural Tissue Engineering.
Pharmaceutics
August 2023
Institute for Stem-Cell Biology, Regenerative Medicine and Innovative Therapies, IRCCS Casa Sollievo della Sofferenza, 71013 San Giovanni Rotondo, Italy.
Spinal cord regeneration using stem cell transplantation is a promising strategy for regenerative therapy. Stem cells transplanted onto scaffolds that can mimic natural extracellular matrix (ECM) have the potential to significantly improve outcomes. In this study, we strived to develop a cell carrier by culturing neural stem cells (NSCs) onto electrospun 2D and 3D constructs made up of specific crosslinked functionalized self-assembling peptides (SAPs) featuring enhanced biomimetic and biomechanical properties.
View Article and Find Full Text PDFLow-power microwaves: a cell-compatible physical treatment to enhance the mechanical properties of self-assembling peptides.
Nanoscale
October 2023
Institute for Stem-Cell Biology, Regenerative Medicine and Innovative Therapies, IRCCS Casa Sollievo della Sofferenza, 71013 San Giovanni Rotondo, Italy.
Article Synopsis
- Biomaterials for tissue engineering need to resemble the native extracellular matrix in both structure and mechanical properties for effective implantation therapies.
- Self-assembling peptides (SAPs) are promising materials but often produce soft hydrogels unsuitable for tougher tissues; chemical cross-linking is a common solution but can involve toxic or expensive agents.
- The study introduces a novel, green method using microwave irradiation to enhance the stiffness and resilience of SAPs without compromising their biomimetic properties, suggesting a feasible approach for broader use in research and clinical applications.
Morphoscanner2.0: A new python module for analysis of molecular dynamics simulations.
PLoS One
May 2023
Center for Nanomedicine and Tissue Engineering (CNTE), A.S.S.T. Grande Ospedale Metropolitano Niguarda, Milan, Italy.
Molecular dynamics simulations, at different scales, have been exploited for investigating complex mechanisms ruling biologically inspired systems. Nonetheless, with recent advances and unprecedented achievements, the analysis of molecular dynamics simulations requires customized workflows. In 2018, we developed Morphoscanner to retrieve structural relations within self-assembling peptide systems.
View Article and Find Full Text PDFA Light Scattering Investigation of Enzymatic Gelation in Self-Assembling Peptides.
Gels
April 2023
Department of Chemistry, Materials Science, and Chemical Engineering (CMIC), Politecnico di Milano, Edificio 6, Piazza Leonardo da Vinci 32, 20133 Milano, Italy.
Self-assembling peptides (SAPs) have been increasingly studied as hydrogel-former gelators because they can create biocompatible environments. A common strategy to trigger gelation, is to use a pH variation, but most methods result in a change in pH that is too rapid, leading to gels with hardly reproducible properties. Here, we use the urea-urease reaction to tune gel properties, by a slow and uniform pH increase.
View Article and Find Full Text PDFThe Anti-Inflammatory Effect of Lactose-Modified Hyaluronic Acid Molecules on Primary Bronchial Fibroblasts of Smokers.
Polymers (Basel)
March 2023
Histology Unit, Department of Molecular Medicine, University of Padova, 35121 Padova, Italy.
The progression of smoking-related diseases is characterized by macrophage-mediated inflammation, which is responsible for an increased expression of proinflammatory cytokines and galectins, molecules that bind specifically to β-galactoside sugars. This study aimed to assess the anti-inflammatory and antioxidant effects of a broad selection of differently lactose-modified hyaluronic acids (HA) named HYLACH, which are able to bind proinflammatory galectins. The best HYLACH ligands for Gal-3 were selected in silico and their activities were tested in vitro on primary human bronchial fibroblasts obtained from smokers and inflamed with the conditioned medium of activated U937 monocytes.
View Article and Find Full Text PDFLong-term cultures of human pancreatic islets in self-assembling peptides hydrogels.
Front Bioeng Biotechnol
February 2023
Institute for Stem-Cell Biology, Regenerative Medicine and Innovative Therapies (ISBReMIT), IRCCS Casa Sollievo della Sofferenza, San Giovanni Rotondo, Italy.
Human pancreatic islets transplantation is an experimental therapeutic treatment for Type I Diabetes. Limited islets lifespan in culture remains the main drawback, due to the absence of native extracellular matrix as mechanical support after their enzymatic and mechanical isolation procedure. Extending the limited islets lifespan by creating a long-term culture remains a challenge.
View Article and Find Full Text PDFStructure-activity relationships of antibacterial peptides.
Microb Biotechnol
April 2023
Institute for Stem-Cell Biology, Regenerative Medicine and Innovative Therapies, IRCCS Casa Sollievo della Sofferenza, San Giovanni Rotondo, Italy.
Antimicrobial peptides play a crucial role in innate immunity, whose components are mainly peptide-based molecules with antibacterial properties. Indeed, the exploration of the immune system over the past 40 years has revealed a number of natural peptides playing a pivotal role in the defence mechanisms of vertebrates and invertebrates, including amphibians, insects, and mammalians. This review provides a discussion regarding the antibacterial mechanisms of peptide-based agents and their structure-activity relationships (SARs) with the aim of describing a topic that is not yet fully explored.
View Article and Find Full Text PDFBioinspired photo-crosslinkable self-assembling peptides with pH-switchable "on-off" luminescence.
Nanoscale Adv
January 2022
Tissue Engineering Unit, Institute for Stem Cell Biology, Regenerative Medicine and Innovative Therapies-ISBReMIT, Fondazione IRCCS Casa Sollievo della Sofferenza 71013 San Giovanni Rotondo FG Italy
Significant progress has been made in peptide self-assembly over the past two decades; however, the cross-linking of self-assembling peptides yielding better performing nanomaterials is still in its infancy. Indeed, self-assembling peptides (SAPs), relying only on non-covalent interactions, are mechanically unstable and susceptible to solvent erosion, greatly hindering their practical application. Herein, drawing inspiration from the biological functions of tyrosine, we present a photo-cross-linking approach for the cross-linking of a tyrosine-containing LDLK12 SAP.
View Article and Find Full Text PDFIdentification of a Novel Pseudo-Natural Product Type IV IDO1 Inhibitor Chemotype.
Angew Chem Int Ed Engl
October 2022
Max Planck Institute of Molecular Physiology, Department of Chemical Biology, Otto-Hahn-Strasse 11, 44227, Dortmund, Germany.
Natural product (NP)-inspired design principles provide invaluable guidance for bioactive compound discovery. Pseudo-natural products (PNPs) are de novo combinations of NP fragments to target biologically relevant chemical space not covered by NPs. We describe the design and synthesis of apoxidoles, a novel pseudo-NP class, whereby indole- and tetrahydropyridine fragments are linked in monopodal connectivity not found in nature.
View Article and Find Full Text PDFEditorial: New Microenvironments for Neuronal Differentiation.
Front Neurosci
April 2022
Center for Nanomedicine and Tissue Engineering, ASST GOM Niguarda and Tissue Engineering Unit, ISBREMIT, IRCCS Casa Sollievo della Sofferenza, Niguarda Ca' Granda Hospital, Milan, Italy.
Boosted Cross-Linking and Characterization of High-Performing Self-Assembling Peptides.
Nanomaterials (Basel)
January 2022
Institute for Stem-Cell Biology, Regenerative Medicine and Innovative Therapies, IRCCS Casa Sollievo della Sofferenza, 71013 San Giovanni Rotondo, Italy.
Tissue engineering (TE) strategies require the design and characterization of novel biomaterials capable of mimicking the physiological microenvironments of the tissues to be regenerated. As such, implantable materials should be biomimetic, nanostructured and with mechanical properties approximating those of the target organ/tissue. Self-assembling peptides (SAPs) are biomimetic nanomaterials that can be readily synthesized and customized to match the requirements of some TE applications, but the weak interactions involved in the self-assembling phenomenon make them soft hydrogels unsuited for the regeneration of medium-to-hard tissues.
View Article and Find Full Text PDFSynthetic scaffolds for 3D cell cultures and organoids: applications in regenerative medicine.
Crit Rev Biotechnol
May 2022
Tissue Engineering Unit, Institute for Stem Cell Biology, Regenerative Medicine and Innovative Therapies-ISBReMIT, Fondazione IRCSS Casa Sollievo della Sofferenza, San Giovanni Rotondo, Italy.
Three-dimensional (3D) cell cultures offer an unparalleled platform to recreate spatial arrangements of cells . 3D cell culture systems have gained increasing interest due to their evident advantages in providing more physiologically relevant information and more predictive data compared to their two-dimensional (2D) counterpart. Design and well-established fabrication of organoids (a particular type of 3D cell culture system) are nowadays considered a pivotal achievement for their ability to replicate cytoarchitecture and the functionalities of an organ.
View Article and Find Full Text PDFHyperBeta: characterizing the structural dynamics of proteins and self-assembling peptides.
Sci Rep
April 2021
Fondazione IRCCS Casa Sollievo della Sofferenza, Unità Ingegneria Tissutale, Viale Cappuccini 1, San Giovanni Rotondo, 71013, Foggia, Italy.
Self-assembling processes are ubiquitous phenomena that drive the organization and the hierarchical formation of complex molecular systems. The investigation of assembling dynamics, emerging from the interactions among biomolecules like amino-acids and polypeptides, is fundamental to determine how a mixture of simple objects can yield a complex structure at the nano-scale level. In this paper we present HyperBeta, a novel open-source software that exploits an innovative algorithm based on hyper-graphs to efficiently identify and graphically represent the dynamics of [Formula: see text]-sheets formation.
View Article and Find Full Text PDFCorrection to Self-Assembling Peptide EAK16 and RADA16 Nanofiber Scaffold Hydrogel.
Chem Rev
April 2021
Laboratory of Molecular Architecture, Media Lab, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139-4307, United States.
Self-assembling peptide scaffolds in the clinic.
NPJ Regen Med
February 2021
Laboratory of Molecular Architecture, Media Lab, Massachusetts Institute of Technology, Cambridge, MA, 02139-4307, USA.
Well-defined scaffold hydrogels made of self-assembling peptides have found their way into clinical products. By examining the properties and applications of two self-assembling peptides-EAK16 and RADA16-we highlight the potential for translating designer biological scaffolds into commercial products.
View Article and Find Full Text PDFAuthor Correction: Control over the fibrillization yield by varying the oligomeric nucleation propensities of self-assembling peptides.
Commun Chem
November 2020
Utrecht Institute for Pharmaceutical Sciences, Department of Pharmaceutics, Faculty of Science, Utrecht University, Universiteitsweg 99, 3584 CG, Utrecht, The Netherlands.
Self-assembling peptide hydrogels for the stabilization and sustained release of active Chondroitinase ABC in vitro and in spinal cord injuries.
J Control Release
February 2021
Tissue Engineering Unit, Institute for Stem Cell Biology, Regenerative Medicine and Innovative Therapies-ISBReMIT, Fondazione IRCCS Casa Sollievo della Sofferenza, San Giovanni Rotondo (FG)., Italy; Center for Nanomedicine and Tissue Engineering (CNTE), ASST Grande Ospedale Metropolitano Niguarda, Milan, Italy. Electronic address:
Activated microglia/macrophages infiltration, astrocyte migration, and increased production of inhibitory chondroitin sulfate proteoglycans (CSPGs) are standard harmful events taking place after the spinal cord injuries (SCI). The gliotic scar, viz. the outcome of chronic SCI, constitutes a long-lasting physical and chemical barrier to axonal regrowth.
View Article and Find Full Text PDFSelf-Assembling Peptide EAK16 and RADA16 Nanofiber Scaffold Hydrogel.
Chem Rev
December 2020
Laboratory of Molecular Architecture, Media Lab, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139-4307, United States.
Short peptides are ubiquitous in nature. They are found as hormones, pheromones, antibacterial and antifungal agents in innate immunity systems, toxins, and pesticides. But no one seriously considered that peptides could be useful as scaffold hydrogel materials.
View Article and Find Full Text PDFControl over the fibrillization yield by varying the oligomeric nucleation propensities of self-assembling peptides.
Commun Chem
November 2020
Utrecht Institute for Pharmaceutical Sciences, Department of Pharmaceutics, Faculty of Science, Utrecht University, Universiteitsweg 99, 3584 CG, Utrecht, The Netherlands.
Self-assembling peptides are an exemplary class of supramolecular biomaterials of broad biomedical utility. Mechanistic studies on the peptide self-assembly demonstrated the importance of the oligomeric intermediates towards the properties of the supramolecular biomaterials being formed. In this study, we demonstrate how the overall yield of the supramolecular assemblies are moderated through subtle molecular changes in the peptide monomers.
View Article and Find Full Text PDFCross-Linked Self-Assembling Peptides and Their Post-Assembly Functionalization via One-Pot and In Situ Gelation System.
Int J Mol Sci
June 2020
Tissue Engineering Unit, Institute for Stem Cell Biology, Regenerative Medicine and Innovative Therapies-ISBReMIT, Fondazione IRCCS Casa Sollievo della Sofferenza, 71013 San Giovanni Rotondo (FG), Italy.
Supramolecular nanostructures formed through peptide self-assembly can have a wide range of applications in the biomedical landscape. However, they often lose biomechanical properties at low mechanical stress due to the non-covalent interactions working in the self-assembling process. Herein, we report the design of cross-linked self-assembling peptide hydrogels using a one-pot in situ gelation system, based on 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide/N-hydroxysulfosuccinimide (EDC/sulfo-NHS) coupling, to tune its biomechanics.
View Article and Find Full Text PDF