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Hairpin DNA-functionalized gold nanorods for mRNA detection in homogenous solution.

J Biomed Opt

September 2016

University of Strathclyde, Photophysics Group, Center for Molecular Nanometrology, Department of Physics, SUPA, John Anderson Building, 107 Rottenrow, Glasgow G4 0NG, United Kingdom.

We report a fluorescent probe for mRNA detection. It consists of a gold nanorod (GNR) functionalized with fluorophore-labeled hairpin oligonucleotides (hpDNA) that are complementary to the mRNA of a target gene. This nanoprobe was found to be sensitive to a complementary oligonucleotide, as indicated by significant changes in both fluorescence intensity and lifetime.

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