[An extended operation for scirrhous gastric cancer--its significance and procedure].

We have been performing an extended operation for scirrhous gastric cancer, namely left upper abdominal exenteration plus Appleby's method, and compared the prognosis among macroscopic subtype classifications. The surgical procedure of left upper abdominal exenteration plus Appleby's method is to resect the neighboring organs of the stomach in an en bloc fashion. It consists of total gastrectomy, partial pancreatectomy, splenectomy, transverse colectomy, cholecystectomy, resection of the left adrenal gland, and ligation and resection of the common hepatic artery.

Liver perfusion chemotherapy via both the hepatic artery and portal vein to prevent hepatic metastasis after extended pancreatectomy for adenocarcinoma of the pancreas.

Since hepatic metastasis is a common cause of treatment failure after curative pancreatectomy for adenocarcinoma of the pancreas, we developed a new method of postoperative hepatic perfusion chemotherapy via both the hepatic artery and portal vein. The present study was conducted to determine if this method decreases the hepatic recurrence and improves the survival rate. Following extended pancreatectomy with wide lymphatic and connective tissue clearance for pancreatic cancer, one catheter was placed in the hepatic artery and one in the portal vein.

Surgery for brain metastases from nonsmall cell lung carcinomas and tissue cultures from the resected specimens.

Between 1978 and 1989, 44 patients underwent 44 thoracotomies and 55 craniotomies for nonsmall cell lung carcinoma (NSCLC) and its brain metastases. Patients ages ranged from 20 to 75 years. There were no intraoperative mortalities.

Prevalence of second generation antibody to hepatitis C virus among voluntary blood donors in Osaka, Japan.

To clarify the demographic characteristics of the prevalence of hepatitis C virus (HCV) infection in Osaka, Japan, where hepatocellular carcinoma is common, we investigated the screening data of antibody to HCV (anti-HCV, DAINABOTHCVPHA, second generation assay) in 197,600 voluntary blood donors residing in Osaka. The study found that age-standardized prevalence of anti-HCV was significantly higher than that of HBsAg (2.25 cf 0.

[A case of hepatocellular carcinoma implantation to chest wall by percutaneous ethanol injection therapy].

A 62-year-old male patient with hepatocellular carcinoma (HCC) received percutaneous ethanol injection therapy (PEIT) four times following fine needle biopsy (FNB). Six months later, he underwent partial hepatic resection because of the insufficient anti-cancer effect of PEIT. One year and five months after PEIT, a subcutaneous tumor, which appeared to be a needle tract seeding by PEIT, was recognized in the 9th intercostal space of the right chest wall.

["Wrapping therapy" for hepatocellular carcinomas with collateral feeders].

A new treatment method to intercept collaterals with silicone rubber sheeting, called "wrapping therapy", was used for 18 patients with advanced hepatocellular carcinomas having collateral feeders from other organs surrounding the liver. Chemoembolization in 17 of the 18 patients in whom it had been conducted more than once, had proven ineffective. Obvious collateral feeders (parasitic branches) to the tumors were recognized on hepatic angiograms in two-thirds of the patients.

[A case of huge liver metastasis from jejunal leiomyosarcoma completely controlled by intra-arterial and intra-portal vein infusion chemotherapy following hepatectomy].

A 39-year-old woman was found to have intrapelvic tumor and multiple liver metastases by abdominal computed tomography. Although laparotomy was done under the diagnosis of ovarian cancer with liver metastases, the histological diagnosis of the tumor during the operation was jejunal leiomyosarcoma. Only the primary lesion was removed at the same time.

[Promotion of hematogenous metastasis in relation to endothelial cell injury by treatment with anticancer drugs].

It has been reported that anticancer drugs at high concentration increase experimental hematogenous metastasis owing to endothelial injury. In this study, we evaluate the effects of anticancer drugs at clinical doses on endothelial cells in the process of metastasis. To do so, we have developed in vitro assay methods which can assess endothelial cell retraction and barrier function against invasion by tumor cells.

[Adjuvant chemo-endocrine chemotherapy with gastrin antagonist after resection of liver metastasis in colorectal cancer].

This study was designed to determine whether or not chemo-endocrine therapy after the resection of liver metastasis from colorectal cancer would prevent re-recurrence in the liver. Postoperatively, seven patients received proglumide gastrin antagonist (1,200 mg/day) +5'-DFUR (800 mg/day) orally for 2 years. MMC 6-10 mg and ADR 20 mg were infused every two weeks alternately for 1 year via catheter in the common hepatic artery.

The detection of breast carcinoma micrometastases in axillary lymph nodes by means of reverse transcriptase-polymerase chain reaction.

Background: The development of a sensitive method for the detection of breast carcinoma micrometastases in axillary lymph nodes is reported.

Methods: The method was based on amplification of MUC1 mRNA, which encodes a core protein of polymorphic epithelial mucin, by a reverse transcriptase-polymerase chain reaction (RT-PCR). Total RNA, which was extracted from a breast carcinoma cell line (MCF-7), primary breast carcinomas, and axillary lymph nodes, was subjected to analysis of MUC1 mRNA expression by the RT-PCR method.

Ornithine decarboxylase inhibitor attenuates bombesin enhancement of intestinal carcinogenesis and metastasis induced by azoxymethane.

The effects of combined administration of bombesin (40 micrograms/kg body weight) and the ornithine decarboxylase (ODC) inhibitor, 1,3-diaminopropane (DAP), on the development of large and small intestinal tumors and the incidence of their metastasis to the peritoneum induced by azoxymethane (AOM, 7.4 mg/kg body weight), the ODC activity of the intestinal wall, and the labeling index of the intestinal mucosa and tumor were investigated in inbred Wistar rats. Rats received weekly s.

Surgical treatment of brain metastases of lung cancer: retrospective analysis of 89 cases.

The records of 89 patients who underwent surgery for solitary or multiple parenchymal brain metastases of lung cancer at the Osaka Center for Adult Diseases between 1978 and 1990 were reviewed with follow up until March 1992. The aim of this retrospective analysis was to identify prognostic features that were associated with a favourable outcome. The benefits of brain tumour surgery were evaluated in terms of the cause of death (brain metastasis, tumour in another organ, or treatment related) as well as the postoperative changes in functional state indicated by the Karnofsky scale.

Discrimination between multicentric and multifocal carcinomas of the breast through clonal analysis.

Background: An unanswered, important question concerning multiple breast carcinomas is whether they arise independently in the breast (multicentric) or are metastatic deposits from a single, primary carcinoma (multifocal). This issue was studied by clonal analysis of each focus of multiple breast carcinomas.

Methods: First, the clonality of 30 breast carcinomas was analyzed by the method based on restriction fragment length polymorphism of the X-chromosome-linked phosphoglycerokinase (PGK) gene and on random inactivation of the gene.

Cytologic examination of pancreatic juice for differential diagnosis of benign and malignant mucin-producing tumors of the pancreas.

Background: A new clinical type of pancreatic tumor, the mucin-producing tumor, has been recognized recently. However, it is not always easy to distinguish benign from malignant tumors preoperatively. In this study, three different methods of differentiating mucin-producing tumors of the pancreas were compared.

[Relationship between physical activity and health examination variables in male workers--new methods to assess physical activity and their applications to epidemiologic research].

Few epidemiological studies investigating the relationship between physical activity and cardiovascular diseases have been performed in Japan. To quantify the physical activity of individuals, a 24-hour activity record was developed to calculate 24-hour energy expenditure for use in an epidemiologic survey. Major physical activity was recorded every 15 minutes during a day and the relative metabolic rate for each physical activity was adopted from data of the Labor Science Institute in Japan.

Necrosis of hepatocellular carcinoma caused by spontaneously arising arterial thrombus.

This paper describes a 65-year-old Japanese man with hepatocellular carcinoma (HCC) in whom the alpha-fetoprotein level decreased remarkably without any treatment. Plain computed tomography disclosed a low-density area in the left lateral segment. Liver scintigraphy revealed a filling defect with 99mTc-Sn colloid and increased uptake of 67Ga-citrate.

Simultaneous Assay of ErbB-2 Protein and Carcinoembryonic Antigen in Cyst Fluid as an Aid in Diagnosing Cystic Lesions of the Breast.

C-erbB-2 oncoprotein (ErbB-2 protein)and carcinoembryonic antigen (CEA)were simultaneously evaluated in the breast cyst fluid of 6 patients with intracystic cancer, 6 patients with intracystic papilloma, and 42 patients with gross cystic disease. A combination test, using 12 ng of ErbB-2 protein and 150 ng of CEA/ml of cyst fluid, respectively, as cutoff values resulted in a positive detection rate of 83%(5/6 cases) for intracystic breast cancer. Two of the 6 patients with intracystic papilloma and 2 of the 42 patients with gross cystic disease were positive.

Characterization of mouse monoclonal antibody B1.4 reactive with human invasive bladder cancer and some other malignant tumors but not with normal urinary epithelium.

Immunohistochemical analysis by indirect immunoperoxidase staining demonstrated that monoclonal antibody (mAb) B1.4 derived from a mouse immunized with a bladder cancer cell line EJ-1 was reactive with a high proportion of high-grade and invasive bladder tumors, but not with the majority of low-grade and superficial bladder tumors, or normal urinary epithelium. Among 71 primary bladder tumors classified by pathological grading, positive stainings were observed in 1 of 34 tumors (3%) of grade 1, 8 of 20 tumors (40%) of grade 2 and 14 of 17 tumors (82%) of grade 3.