5 results match your criteria: "Cell Analysis Center[Affiliation]"
Eosinophil extracellular trap cell death-derived DNA traps: Their presence in secretions and functional attributes.
J Allergy Clin Immunol
January 2016
Divisions of Allergy and Inflammation and Infectious Diseases, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Mass.
Background: Activated human eosinophils, as well as neutrophils, can release extracellular chromatin to form DNA traps through cytolytic extracellular trap cell death (ETosis). Although formations of neutrophil DNA traps are recognized in patients with various inflammatory conditions, neither the presence of ETosis-derived eosinophil DNA traps in human allergic diseases nor the characteristics of these DNA traps have been studied.
Objective: We investigated the presence of ETosis-derived DNA traps in eosinophil-rich sinus and ear secretions and the functional attributes of ETosis DNA traps.
Heme-related molecules induce rapid production of neutrophil extracellular traps.
Transfusion
November 2014
Cell Analysis Center, Scientific Affairs, Sysmex Corporation, Kobe, Japan.
Background: Pulmonary endothelial cell damages caused by neutrophil overactivation could result in acute lung injuries including transfusion-related acute lung injury (TRALI). We previously reported that heme-related molecules derived from hemolysis induced the production of reactive oxygen species from neutrophils. Recently, neutrophil extracellular traps (NETs) have been demonstrated to associate with the onset of TRALI.
View Article and Find Full Text PDFNon-activated T and B lymphocytes become morphologically distinguishable after detergent treatment.
Cytometry A
April 2013
Cell Analysis Center, Scientific Affairs, Sysmex Corporation, Japan.
T and B lymphocytes are difficult to distinguish morphologically even with electron microscopy, and antibodies are generally used to make the distinction. A specific reagent, consisting of nonionic and cationic detergents, is used for leukocyte differentiation using the Sysmex automated blood analyzer. This reagent increases cell membrane porosity and enables the introduction of fluorescent dye into leukocytes.
View Article and Find Full Text PDFRapid discrimination of Gram-positive and Gram-negative bacteria in liquid samples by using NaOH-sodium dodecyl sulfate solution and flow cytometry.
PLoS One
March 2013
Cell Analysis Center, Scientific Affairs, Sysmex Corporation, Nishi-ku, Kobe, Hyogo, Japan.
Article Synopsis
- The study aimed to develop a quick method to differentiate between gram-positive and gram-negative bacteria in liquid samples for accurate UTI diagnosis and treatment.
- The researchers used a NaOH-SDS solution to test the different responses of bacteria based on their cell wall structures, finding that gram-negative bacteria like E. coli were easily lysed, while gram-positive bacteria like Enterococcus faecalis were not.
- The optimized method allowed for this distinction to be made in just 10 minutes, potentially aiding in faster and more effective treatment decisions for UTI patients.
Morphological definition of CD71 positive reticulocytes by various staining techniques and electron microscopy compared to reticulocytes detected by an automated hematology analyzer.
Clin Chim Acta
June 2009
Cell Analysis Center, Scientific Affairs, Sysmex Corporation, 4-4-4 Takatsukadai, Nishi-ku, Kobe 651-2271, Japan.
Background: The enumeration of peripheral blood reticulocytes plays an important part in clinical hematology. Although reticulocyte enumeration is currently performed with visible dyes such as New Methylene Blue (NMB), fluorescent dyes, or anti-CD71 (transferrin receptor) antibody, it has not been demonstrated whether the reticulocytes detected in each method are the same or not.
Methods: We prepared the reticulocyte rich fraction with density gradient centrifugation, stained with both anti-CD71 and Sysmex's fluorescent stain RET SEARCH (II), and detected the cells by both confocal laser scanning microscopy and flow cytometry.