2 results match your criteria: "Cargill Biotechnology Development Center[Affiliation]"
Metagenomic discovery of feruloyl esterases from rumen microflora.
Appl Microbiol Biotechnol
October 2019
Cargill Biotechnology Development Center, Minneapolis, MN, USA.
Article Synopsis
- Feruloyl esterases (FAEs) are important enzymes involved in breaking down ferulic acid links in plant fibers, which is crucial for energy conversion in the cow's rumen.
- Seven active phagemids were identified from the rumen microbial metagenome, showing genetic sequences related to known FAEs and being expressed and purified in E. coli.
- The study found diverse FAEs that can cleave different substrates, highlighting their role in efficient fiber digestion within the rumen's complex microbial environment.
A novel method for rapid and sensitive metagenomic activity screening.
MethodsX
June 2018
Cargill Biotechnology Development Center, Minneapolis, MN, USA.
Direct cloning of metagenomes has proven to be a powerful tool for the exploration of the diverse sequence space of a microbial community leading to gene discovery and biocatalyst development. The key to such approach is the development of rapid, sensitive, and reliable functional screening of libraries. The majority of library screen have relied on the use of agar plates in petri dishes incorporating the target enzyme substrate for activity detection of positive clones (Iqbal et al.
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