[Effect of RBC lysing solution on CD34(+) cell counting].

To investigate whether the RBC lysing solution can affect the results of relative enumeration of CD34(+) cells, 37 mobile peripheral blood apheresis products were stained using CD34-PE and CD45-FITC monoclone antibodies and RBCs were then lysed by two lysing solution commercially available (one named FACS Lysing Solution, FACS; another IOTest 3 Lysing Solution, IOTest) and one lysing solution self-prepared. After being processed by lyse-and-then-washed method, samples were detected by FACSC anto flow cytometer. The percentages of CD34(+) cells were determined based on ISHAGE gating strategy, forward and side scatter (FSC and SSC) characteristics, percentage of CD45(+) cells were recorded simultaneously.

[Prevention and cure sinusitis complicated by endoscopic transnasal approach in surgical treatment of pituitary adenoma].

Objective: To recognize the causes, methods of prevention and cure of sinusitis complicated by endoscopic transnasal approach in surgical treatment of pituitary adenomas.

Method: The data of 187 patients who underwent surgical treatments of pituitary adenomas through endoscopic transnasal approach were collected, and the surgical complications were analyzed.

Result: Endoscopic transnasal transsphenoidal approach were used successfully in 187 cases, twelve cases (6.

[Prospective study on associations between levels of total cholesterol, triglyceride and risk of ischemic and hemorrhagic strokes].

Objective: To explore associations between levels of total cholesterol (TC), triglyceride (TG) and incidence of ischemic and hemorrhagic strokes in populations.

Methods: Baseline investigations on stroke-related risk factors and physical examinations were performed in 10 093 (> 35 years) stroke-free urban community residents from 5 cities in China during May to July in 1987, follow-up investigations on stroke events were made during 1998 to 2000. The hazard ratios and 95% confidence intervals (CI) of ischemic and hemorrhagic strokes in middle, high tertiles of baseline TC or TG levels were compared with low baseline tertile residents using the Cox regression model.

[Stat3 antisense oligodeoxynucleotide induces apoptosis in laryngeal carcinoma cells by regulating apoptosis-related factors].

Objective: To study the mechanism of apoptosis in laryngeal carcinoma cell induced by Stat3 antisense oligodeoxynucleotide (ASODN).

Method: The designed Stat3 ASODN was transferred into laryngeal carcinoma Hep-2 cell by lipofection. Expression of Bcl-2, Bax and C-Myc were detected by Western blot and PCR.

Bisphenol A in combination with TNF-alpha selectively induces Th2 cell-promoting dendritic cells in vitro with an estrogen-like activity.

Bisphenol A (BPA) is a monomer used in manufacturing a wide range of chemical products, including epoxy resins and polycarbonate. BPA, an important endocrine disrupting chemical that exerts estrogen-like activities, is detectable at nanomolar levels in human serum worldwide. The pregnancy associated doses of 17beta-estradiol (E2) plus tumor-necrosis factor-alpha (TNF-alpha) induce distorted maturation of human dendritic cells (DCs) that result in an increased capacity to induce T helper (Th) 2 responses.

[Analysis of imaging and clinical feature of leukoencephalopathy with hearing loss first complaint].

Objective: To investigate the imaging and clinical feature of leukoencephalopathy with hearing loss first complaint.

Method: The head MRI and clinical feature of 7 cases leukoencephalopathy with hearing loss first complaint were retrospectively analysed, including their place, shape, range and blood plasma very-long-chain fatty acid (VLCFA).

Result: The head MRI of 2 cases shows symmetry distributed long T, and T2 signal like butterfly aliform in white matter beside both cornu posterior ventriculi lateralis.

Anatomical study of forearm arteries with ultrasound for percutaneous coronary procedures.

Background: In recent years, the radial artery (RA) has become an alternative vascular access site for percutaneous coronary procedures, and the ulnar artery (UA) is another possibility. The objective of this study was to investigate the anatomy of the forearm arteries with ultrasound (US) and to evaluate the effect of the anatomy of the right RA (RRA) on the outcomes of transradial coronary procedures.

Methods And Results: The 638 patients undergoing transradial coronary procedures were examined with US for measurement of the diameters of the forearm arteries and determination of their anatomical abnormalities before the procedures.

Comb-shaped conjugates comprising hydroxypropyl cellulose backbones and low-molecular-weight poly(N-isopropylacryamide) side chains for smart hydrogels: synthesis, characterization, and biomedical applications.

Hydroxypropyl cellulose (HPC) possesses a lower critical solution temperature (LCST) above 40 °C, while the poly(N-isopropylacrylamide) (P(NIPAAm)) exhibits a LCST of about 32 °C. Herein, comb-shaped copolymer conjugates of HPC backbones and low-molecular-weight P(NIPAAm) side chains (HPC-g-P(NIPAAm) or HPN) were prepared via atom transfer radical polymerization (ATRP) from the bromoisobutyryl-functionalized HPC biopolymers. By changing the composition ratio of HPC and P(NIPAAm), the LCSTs of HPNs can be adjusted to be lower than the body temperature.

[Effects of hypertension state induced by norepinephrine on liver in a swine model of cardiopulmonary resuscitation].

Objective: To study the effects of norepinephrine (NE)-induced hypertension (HT) on hepatic function and pathology after restoration of spontaneous circulation (ROSC) by cardiopulmonary resuscitation (CPR) in swine.

Methods: After 4 minutes of induced ventricular fibrillation (VF), standard CPR was carried out, and then the NE was pumped after ROSC. The survivors were then divided into two groups by the random digits table.

[Extracorporeal membrane oxygenation support in acute cardiorespiratory function failure patients after cardiac surgery].

Objective: To investigate the treatment experience of extracorporeal membrane oxygenation (ECMO) support after cardiac surgery.

Methods: Retrospectively analyze the clinical data of 117 postoperative patients supported with ECMO in cardiac intensive care unit from March 2005 to June 2008. There were 32 female and 85 male patients, with a mean age of (48.

[An analysis of acute myocardial infarction in women aged 40 or less.].

Objective: To analyze the clinical characteristics and angiographic features of myocardial infarction in women aged 40 or less.

Methods: The clinical information and angiographic images of 13 young female patients with myocardial infarction treated in Beijing Anzhen Hospital during the past 6 years were collected and analyzed.

Results: The traditional risk factors especially hypertension and dyslipidaemia were more frequent in young female patients with myocardial infarction.

[The present status of the use of aspirin for secondary prevention of coronary artery disease].

Objective: To examine the current status of the use of aspirin among outpatients with established coronary heart disease (CHD) in China.

Methods: Sixty-four hospitals across 31 provinces of China, including 32 secondary hospitals and 32 tertiary hospitals were selected for a baseline survey. Fifty outpatients with history of acute coronary syndrome (ACS) were recruited consecutively in each hospital.

[Clinical significance of preventive treatment of thrombosis for patients undergoing gynecological surgery with high risk factors].

Objective: To explore the clinical significance of preventive treatment of thrombosis for patients undergoing gynecological surgeries with high risk factors.

Methods: Prospectively, randomized and cases controlled study was performed in 143 patients underwent gynecological surgery with high risk factors to explore the prevention effectiveness and safety in morbidity of lower extremity deep venous thrombosis (LDVT) and relative effected factors, who were enrolled to three groups according to different preventive treatment: group using intermittent pneumatic calf compression (IPC) in 47 cases, group using low-molecular-weight hepairin (LMWH) in 48 cases and control group in 48 cases with no prevention strategy.

Results: The morbidity of LDVT on lower extremity was 6% (6/94) in group IPC and 1% (1/96) in group LMWH, 18% (17/96) in control group, which was obviously reduced in group IPC and group LMWH than that in control group (P < 0.

[Effectiveness of ezetimibe in improving lipid goal attainment in patients with coronary heart diseases treated with statin].

Objective: To investigate the efficacy and safety of ezetimibe on uncontrolled LDL-C when added to ongoing statin therapy in patients with coronary heart diseases (CHD) and diabetes mellitus (DM).

Methods: 61 patients with LDL-C level exceeding target goal (<2.07 mmol/L) after 12 weeks' treatment with statins, received ezetimibe in addition to their ongoing statin therapy for 8 weeks.

Anthropomorphic measurements and general and ocular parameters in adult Chinese: the Beijing Eye Study.

Purpose: To determine whether anthropomorphic measurements are associated with ocular and general parameters.

Methods: The population-based Beijing Eye Study, which included 4439 participants (age > 40 years) out of 5324 individuals invited to be examined in 2001, was repeated in 2006 with 3251 (73.2% of 4439, or 61.

Impact of transradial coronary procedures on radial artery.

Objective: We evaluated the impact of transradial coronary procedures on the radial artery (RA).

Methods: A total of 355 patients who underwent the transradial coronary procedures including transradial coronary angiography (CAG) and percutaneous coronary intervention (PCI) were enrolled. The right RA (RRA) was examined by ultrasound.

Major eye diseases and risk factors associated with systemic hypertension in an adult Chinese population: the Beijing Eye Study.

Purpose: To assess the relationship of hypertension with major eye diseases and other ocular parameters.

Design: Population-based study.

Participants: The Beijing Eye Study is a population-based study that included 4439 Chinese subjects examined at the baseline examination in 2001; there was a follow-up examination in 2006, in which 3251 subjects participated, of whom 3222 had blood pressure measurements.

Use of the spiral flap for closure of small defects of the lower eyelid.

Various options have been proposed for lower eyelid defects depending on the location and the size of the defects. To demonstrate a modification to the traditional rotation flap. The incision was made parallel to the free margin of the lower eyelid.

[Association between body mass index and ten-year-accumulative-risk of hypertension].

Objective: To explore the association between body mass index (BMI) and ten-year accumulative risk of hypertension.

Methods: A prospective study of relationship between blood pressure and other traditional risk factors was carried out in a cohort with 2115 people aged 35-64 years old involved, in 1992 and 2002. The association between baseline BMI level and a ten-year accumulative risk of hypertension was analyzed.

[Pathologic diagnosis of specific infections in upper aerodigestive tract].

Objective: To find a fast and simple method for detection of specific pathogens in upper aerodigestive tract.

Methods: Sixty-one cases of specific infections in upper aerodigestive tract encountered during a 10-year period in Beijing Tongren Hospital were retrospectively studied. Six histochemical stains, including PAS, Giemsa, Gram, methylene blue, modified Warthin-Starry and acid-fast stains were applied.

[Prevalence, awareness, and treatment of hypercholesterolemia among inpatients with acute coronary syndrome in China].

Objective: To estimate the current prevalence, awareness and treatment status of hypercholesterolemia among inpatients with acute coronary syndrome (ACS) in China.

Methods: Sixty-four hospitals across China, including 32 secondary hospitals and 32 tertiary hospitals were selected for baseline survey. Fifty inpatients diagnosed with ACS were recruited consecutively in each participated hospitals.

[Clinical outcome of mitral valve repair in primary infective endocarditis with mitral insufficiency].

Objective: To study the clinical results of mitral valve repair in patients of primary infective endocarditis with mitral insufficiency.

Methods: From January 2004 to July 2007, 40 patients who had undergone valve repair procedure for infective endocarditis with mitral insufficiency were analyzed retrospectively. There were 26 male and 14 female patients, with an average age of (34.

Distribution of bacterial keratitis and emerging resistance to antibiotics in China from 2001 to 2004.

Objective: To study on the distribution of bacterial keratitis isolates and the resistance to antibiotics in China from 2001 to 2004.

Methods: 1985 specimens from the bacterial keratitis at the Beijing Tong Ren Eye Center were cultured and identified. In vitro susceptibility testing of positive isolates to antibiotics was determined by the Kirby-Bauer disk diffusion method and interpreted according to Clinical and Laboratory Standards Institute.