Bees on the run: spp. (Microsporidia) in and related products, Italy.

Introduction: The decline of the European/western honeybee () population is on account of a plethora of microorganisms, such as and , two microsporidian fungi responsible of nosemosis that affects welfare and production of the bee industry. Accordingly, this study aimed to investigate the presence of both pathogens in bees, pollen and honey from apiaries in Southwestern Italy.

Methods: From March to July 2022 and 2023, apiaries ( = 10) were selected and classified as High Impact Areas (HIAs,  = 5) and Low Impact Areas (LIAs,  = 5) according to a 5-point environmental risk index based on factors affecting bee health sand related productions.

Supercritical fluid extrusion of pea flour and pea protein concentrate: Effects on off-flavor removal and sensory improvement.

This study was intended to provide a novel process that fills a knowledge gap in relation to the enhancement of pulses utilization. The primary goal was to develop an experimental framework for using a high-pressure supercritical fluid extruder (SCFX) as a continuous bioreactor to produce off-flavor reduced and functionally superior pulse flours and protein concentrates in a single step. The current study focused on using SCFX processing to remove off-flavor from pulse flour and protein concentrates, enhancing the quality, acceptability, and marketability of pulse-based products.

Incidence and Factors in Delayed Neurological Deficits after Subarachnoid Hemorrhage in Mice.

Background: Delayed cerebral ischemia (DCI) is one of the most feared complications in aneurysmal subarachnoid hemorrhage (SAH). Animal models are crucial to studying the disease mechanisms and potential treatments. DCI in rodents was thought to not exist; herein we examine literature and our experience with DCI in rodents.

Deep Learning-Enabled Integration of Histology and Transcriptomics for Tissue Spatial Profile Analysis.

Spatially resolved transcriptomics enable comprehensive measurement of gene expression at subcellular resolution while preserving the spatial context of the tissue microenvironment. While deep learning has shown promise in analyzing SCST datasets, most efforts have focused on sequence data and spatial localization, with limited emphasis on leveraging rich histopathological insights from staining images. We introduce GIST, a deep learning-enabled gene expression and histology integration for spatial cellular profiling.

Excitation Wavelength Optimization of Europium-Doped Carbon Dots (Eu-CDs) for Highly Sensitive Detection of Tetracyclines in Water.

Antibiotic contamination of water bodies has become a serious problem, which leads to aquatic life pollution and the development of antibiotic resistance. Hence, development of highly sensitive and selective optical sensors for antibiotic detection is at the forefront of scientific research. In this study, we present the synthesis of europium-doped carbon dots (Eu-CDs) and excitation wavelength optimization for the highly sensitive detection of tetracycline (TC) and TC-family antibiotics in water.

Preservation of sexual function with Optilume-a novel treatment for lower urinary tract symptoms secondary to benign prostatic hyperplasia.

Background: The Optilume benign prostatic hyperplasia (BPH) catheter system is a novel minimally invasive paclitaxel-coated dilation system that has demonstrated substantial and durable results in urinary flow and symptoms.

Aim: We now assess the impact of Optilume BPH procedure on sexual function.

Methods: Prior published results have described the methods of data collection during the PINNACLE study, which demonstrated durable improvement in urinary symptoms.

Reference genome of Calochortus tolmiei Hook. & Arn. (Liliaceae), a cat's ear mariposa lily.

Calochortus tolmiei Hook. & Arn., a bulbous monocot with cat's ear flowers in the angiosperm family Liliaceae, is a perennial herb native to northern California, Oregon, and Washington.

Reconstructing the first COVID-19 pandemic wave with minimal data in England.

Accurate measurement of exposure to SARS-CoV-2 in the population is crucial for understanding the dynamics of disease transmission and evaluating the impacts of interventions. However, it was particularly challenging to achieve this in the early phase of a pandemic because of the sparsity of epidemiological data. We previously developed an early pandemic diagnostic tool that linked minimum datasets: seroprevalence, mortality and infection testing data to estimate the true exposure in different regions of England and found levels of SARS-CoV-2 population exposure to be considerably higher than suggested by seroprevalence surveys.

Spoilage investigation of pasteurized apple juice with visual defects identifies a potentially novel Acetobacter species as the primary spoilage agent.

Jellified materials were observed in spoiled pasteurized apple juice that contained dimethyl dicarbonate (DMDC). Microbiological analysis showed a high microbial load (4-5 log CFU/mL) in the sample. Acetobacter spp.

Advantages and disadvantages of current human enteric virus surrogates in soils and aquifers.

Groundwater is one of the main sources of drinking water, thus, human enteric viruses in groundwater could pose safety risks. Many enteric viruses enter drinking water sources through irrigation or recharge of contaminated water. It is therefore advised to test the potential transport risk with harmless surrogates before wastewater or recycled water is used for irrigation or groundwater recharge.

Profiling Tel1 Signaling Reveals a Non-Canonical Motif Targeting DNA Repair and Telomere Control Machineries.

The stability of the genome relies on Phosphatidyl Inositol 3-Kinase-related Kinases (PIKKs) that sense DNA damage and trigger elaborate downstream signaling responses. In S. cerevisiae, the Tel1 kinase (ortholog of human ATM) is activated at DNA double strand breaks (DSBs) and short telomeres.

Presentation, management and outcomes of ruptured intracranial aneurysms in Africa: A systematic review and meta-analysis.

Background: Approximately two percent of the world's population are affected by intracranial aneurysms (IAs). This study aimed to evaluate literature regarding presentation, treatment and outcomes of ruptured IAs in Africa.

Methods: A systematic review of the literature using PubMed/MEDLINE, SCOPUS, Web of Science and Google Scholar databases was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines.

Multiplexed transcriptomic analyzes of the plant embryonic hourglass.

Zoologists have adduced morphological convergence among embryonic stages of closely related taxa, which has been called the phylotypic stage of embryogenesis. Transcriptomic analyzes reveal an hourglass pattern of gene expression during plant and animal embryogenesis, characterized by the accumulation of evolutionarily older and conserved transcripts during mid-embryogenesis, whereas younger less-conserved transcripts predominate at earlier and later embryonic stages. In contrast, comparisons of embryonic gene expression among different animal phyla describe an inverse hourglass pattern, where expression is correlated during early and late stages but not during mid-embryo development.

Onboarding Dairy Farm Employees: Improving the New Employee Experience.

The first days and weeks on the job set the course for a new dairy farm employee. This project involved an educational intervention to increase the use of new employee onboarding practices in dairy farms and analyzes the resulting effects on (1) levels of onboarding practice use, (2) manager perceptions of employee performance, (3) manager satisfaction with the onboarding program, (4) manager concerns about compliance with state and federal employment regulations, and (5) employee turnover. Onboarding advisors (educators and consultants) provided templates, examples, and intensive facilitation directly with farm managers to learn and adopt onboarding practices.

Nitrous oxide production via enzymatic nitroxyl from the nitrifying archaeon .

Ammonia oxidizing archaea (AOA) are among the most abundant microorganisms on earth and are known to be a major source of nitrous oxide (NO) emissions, although biochemical origins of this NO remain unknown. Enzymological details of AOA nitrogen metabolism are broadly unavailable. We report the recombinant expression, purification, and characterization of a multicopper oxidase, Nmar_1354, from the AOA .

Insecticides may facilitate the escape of weeds from biological control.

Background: Preventative pesticide seed treatments (hereafter preventative pest management or PPM) are common corn and soybean treatments, and often include both fungicides and neonicotinoid insecticides. While PPM is intended to protect crops from soil-borne pathogens and early season insect pests, these seed treatments may have detrimental effects on biological control of weed seeds by insects.

Methods: Here, in two 3-year corn-soy rotations in Pennsylvania USA, we investigated a PPM approach to insect management compared to an integrated pest management approach (IPM) and a "no (insect) pest management" (NPM) control.

Centering historically minoritized populations to design effective messages about an evidence-based policy to advance social equity.

Researchers have raised concerns that messages describing racial disparities in social outcomes can reduce or polarize support for public policies to address inequality. We questioned this assumption by testing the impact of carefully crafted messages about child tax credit (CTC) expansion. We conducted two randomized message trials, study 1 using Prolific's nonprobability panel ( = 1,402) and study 2 using SSRS's Opinion Panel, a web-based probability sample of US adults ( = 4,483).

Neonatal inflammation and near-term white matter microstructure in infants born very preterm.

Background: Severe neonatal inflammatory conditions in very preterm infants (VPT: <32 weeks gestational age, GA) are linked to adverse neurodevelopmental outcomes. Differences in white matter (WM) microstructure of the corpus callosum (CC) have been observed at age 6 in VPT children with a history of severe neonatal inflammation. The goal of this study was to determine whether these CC differences can be detected at term-equivalent age using diffusion MRI (dMRI), and whether neonatal inflammation is associated with altered WM in additional tracts implicated in the encephalopathy of prematurity.

Frequent, heterogenous fire supports a forest owl assemblage.

Fire shapes biodiversity in many forested ecosystems, but historical management practices and anthropogenic climate change have led to larger, more severe fires that threaten many animal species where such disturbances do not occur naturally. As predators, owls can play important ecological roles in biological communities, but how changing fire regimes affect individual species and species assemblages is largely unknown. Here, we examined the impact of fire severity, history, and configuration over the past 35 years on an assemblage of six forest owl species in the Sierra Nevada, California, using ecosystem-scale passive acoustic monitoring.

Ancient reticulation, incomplete lineage sorting and the evolution of the pyrenoid at the dawn of hornwort diversification.

Background And Aims: Resolving the phylogeny of hornworts is critical in understanding the evolution of key morphological characters that are unique to the group, including the pyrenoid. Extensive phylogenomic analyses have revealed unexpected complexities in the placement of Leiosporoceros, the previously identified sister taxon to other hornworts. We explore the role of incomplete lineage sorting (ILS) and ancient reticulation in resolving interrelationships and comprehending the diversification and evolutionary processes within hornworts.

Olutasidenib in combination with azacitidine induces durable complete remissions in patients with relapsed or refractory mIDH1 acute myeloid leukemia: a multicohort open-label phase 1/2 trial.

Background: Olutasidenib is a potent, selective, oral, small molecule inhibitor of mutant IDH1 (mIDH1) which induced durable remissions in high-risk, relapsed/refractory (R/R) mIDH1 AML patients in a phase 1/2 trial. We present a pooled analysis from multiple cohorts of the phase 1/2 trial of patients with R/R AML who received combination olutasidenib and azacitidine therapy.

Methods: Adult patients with mIDH1 AML received 150 mg olutasidenib twice daily plus standard-of-care azacitidine (OLU + AZA) and were evaluated for response and safety.

Mechanically interlocked two-dimensional polymers.

Mechanical bonds arise between molecules that contain interlocked subunits, such as one macrocycle threaded through another. Within polymers, these linkages will confer distinctive mechanical properties and other emergent behaviors, but polymerizations that form mechanical bonds efficiently and use simple monomeric building blocks are rare. In this work, we introduce a solid-state polymerization in which one monomer infiltrates crystals of another to form a macrocycle and mechanical bond at each repeat unit of a two-dimensional (2D) polymer.