Migration of bone marrow-derived mesenchymal stem cells induced by tumor necrosis factor-alpha and its possible role in wound healing.

We aimed to investigate the effect of tumor necrosis factor-alpha (TNF-alpha) on the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in the migration ability of mesenchymal stem cells (MSCs) in the context of wound healing. We also explored the role of p38 mitogen-activated protein kinase and extracellular signal-regulated kinase (ERK) signaling pathways in the migration of MSCs. MSCs were isolated from the bone marrow and cultured.

Mesenchymal stem cells and skin wound repair and regeneration: possibilities and questions.

Adult mesenchymal stem cells (MSCs) have the capacity for self-renewal and for differentiating into a variety of cells and tissues. They may leave their niche to migrate to remote tissues and play a critical role in wound repair and tissue regeneration. Because of their multipotency, easy isolation and culture, highly expansive potential, and immunosuppression properties, these cells may be an attractive therapeutic tool for regenerative medicine and tissue engineering.

Anaerobic digestion of municipal solid waste and agricultural waste and the effect of co-digestion with dairy cow manure.

Anaerobic digestion of dairy cow manure (CM), the organic fraction of municipal solid waste (OFMSW), and cotton gin waste (CGW) was investigated with a two-phase pilot-scale anaerobic digestion (AD) system. The OFMSW and CM were digested as single wastes and as combined wastes. The single waste digestion of CM resulted in 62m3 methane/ton of CM on dry weight basis.

Adipose tissue extract enhances skin wound healing.

The main function of adipose tissue has been considered as storage of triglycerides. Adipose tissue was considered harmful for healing extensive and deep burns because of poor circulation and easy liquefaction in wound beds, which offer an excellent culture medium for bacteria. However, these traditional concepts have been challenged with the discovery of the endocrine function of adipose tissue.

Pseudoepitheliomatous hyperplasia formation after skin injury.

Pseudoepitheliomatous hyperplasia (PEH) is characteristic of reactive epithelial downward proliferation into the dermis. To explore the mechanism of PEH formation, we investigated the cause, clinical characteristics, histopathologic change, and clinical treatment of PEH, the expression characteristics of stem cell factor (SCF), and its receptor c-Kit in PEH and normal skin of PEH edge (PEH-N). The clinical data of 11 patients with PEH were reviewed, and biopsy examination and bacterial cultures were performed.

An SCN9A channelopathy causes congenital inability to experience pain.

The complete inability to sense pain in an otherwise healthy individual is a very rare phenotype. In three consanguineous families from northern Pakistan, we mapped the condition as an autosomal-recessive trait to chromosome 2q24.3.

Potentiality of mesenchymal stem cells in regeneration of sweat glands.

Sweat glands play some key roles in homeostasis maintenance and body temperature regulation. In full-thickness burn wounds, sweat glands cannot regenerate to form their three-dimensional organization via the division and terminal differentiation of sweat gland cells. However, the plasticity of mesenchymal stem cells (MSCs) may offer the hope and potentiality to regenerate sweat glands after severe burn injury.

Enhanced wound-healing quality with bone marrow mesenchymal stem cells autografting after skin injury.

Adult stem cells exist in various tissues and organs and have the potential to differentiate into different cell lineages, including bone, cartilage, fat, tendon, muscle, and epithelial cells of the gastrointestinal tract. Here, we report that the in vitro expanded and purified bone marrow mesenchymal stem cells (MSCs) might take on phenotypes with characteristics of vascular endothelial cells (7% on day 3 and 15% on day 1) or epidermal cells (3% on day 3 and 13% on day 1) after being cultured under different lineage-specific culture conditions. Also, in vivo grafting experiments showed that 5-bromodeoxyuridine-labeled MSCs could convert into the phenotypes of vascular endothelial cells (3.

[Effects of the nerve, endocrine, and immune factors on the skin repair and regeneration].

Objective: To investigate the effects of the nerve, endocrine, and immune factors on the process of wound healing and regeneration of the skin after injury and to review the research work in the past years in this area.

Methods: The prospective study was made to explore the relationship among the nerve, endocrine, immune factors, and skin tissue repair and regeneration, and to summarize the recent advance in this area.

Results: The nerve, endocrine, and immune factors played an important role in the repair and generation of the skin after injury.

Engineered growth factors and cutaneous wound healing: success and possible questions in the past 10 years.

In the past 10 years, many engineered growth factors, including recombinant human epidermal growth factor, basic fibroblast growth factor, and platelet-derived growth factor, have been produced and used in the clinic. After screening the results from different centers, some results are found to be encouraging, while others are discouraging. Although the interpretation of these results may depend on your perspective, it may also depend on different criteria, different wounds, and even different aims.

[Different expression of chaperone interacting protein in normal, scar and chronic ulcer tissues and its relationship with wound healing].

Objective: To explore the expression characteristics of chaperone interacting protein (CHIP) in normal, scar and chronic ulcer tissues and its relationship with wound healing.

Methods: Twenty biopsies including scar tissues (n = 8), chronic ulcer tissues (n = 4) and normal tissues (n = 8) were used in this study. The immunohistochemical staining (power vision two-step histo-staining reagent) was used to explore the amount and expression characteristics of such protein.

Morphological and distribution characteristics of sweat glands in hypertrophic scar and their possible effects on sweat gland regeneration.

Background: In hypertrophic scar tissue, no sweet gland and hair follicle exist usually because of the dermal and epidermal damage in extensive thermal skin injury, thus imparing regulation of body temperature. This study was designed to reveal the morphological and distributional characteristics of the sweat glands in normal skin and hypertrophic scar obtained from children and adults, and to study the possible interfering effects of the scar on regeneration of the sweat gland after burn injury.

Methods: Biopsies of hypertrophic scar were taken from four children (4 - 10 years) and four adults (35 - 51 years).

[Growth factors and wound healing: review and prospect in recent ten years].

Objective: To review the current condition of growth factors and their application to clinical treatment of acute and chronic wounds.

Methods: Data from the literature and Medline were analyzed according to their different uses in acute and chronic wounds. Their potential side-effects were studied.

Enhanced anti-apoptosis and gut epithelium protection function of acidic fibroblast growth factor after cancelling of its mitogenic activity.

Aim: Mitogenic and non-mitogenic activities of fibroblast growth factor (FGF) are coupled to a range of biological functions, from cell proliferation and differentiation to the onset of many diseases. Recent reports have shown that acidic fibroblast growth factor (aFGF) has a powerful anti-apoptosis function, which may have potentially therapeutical effect on gut ischemia and reperfusion injuries. However, whether this function depends on its mitogenic or non-mitogenic activity remains unclear.

[Enhancing the repair quality of skin injury on porcine after autografting with the bone marrow mesenchymal stem cells].

Objective: To explore the effects of bone marrow mesenchymal stem cells (MSCs) on the quality of healing of porcine skin wounds after burn injury so as to provide a new method for clinical skin repair in the future.

Methods: Seventy-two deep-partial thickness burn wounds were produced on the back of 6 minipigs and then the pigs were randomly divided into 6 groups: saline control, MSCs treatment, MSCs plus basic fibroblast growth factor (bFGF) treatment, MSCs plus epidermal growth factor (EGF) treatment, bFGF treatment or EGF treatment only. MSCs were isolated from porcine marrow and cultured in vitro.

[Study on combined implantation of pig dermis and autologous skin in rats].

In this study the treatment effect of combined implantation of autologous skin on pig dermis in injured rats was observed. Twenty-one Wistar rats were used, and the wounds were formed by excising a piece of full thickness skin on the back. After the pig dermis was implanted, the autologous skin was grafted on the dermis at 0.

Activation of phosphorylating-p38 mitogen-activated protein kinase and its relationship with localization of intestinal stem cells in rats after ischemia-reperfusion injury.

Aim: To investigate the expression of phosphorylating p38 mitogen-activated protein kinase (MAPK) in rat small intestine after ischemia-reperfusion (I/R) insult and its relationship with the localization of intestinal stem cells.

Methods: Forty-eight Wistar rats were divided randomly into three groups, namely intestinal ischemia-reperfusion group (R), intestinal ischemia group (I) and sham-operated control group (C). In group I, the animals were killed 45 minutes after superior mesenteric artery (SMA) occlusion, while in group R the rats sustained SMA occlusion for 45 minutes and reperfusion for 2, 6, 12 or 24 hours respectively.

Rapid mitogen-activated protein kinase by basic fibroblast growth factor in rat intestine after ischemia/reperfusion injury.

Aim: Previous studies showed that exogenous basic fibroblast growth factor (bFGF or FGF-2) could improve physiological dysfunction after intestinal ischemia/ reperfusion (I/R) injury. However, the mechanisms of this protective effect of bFGF are still unclear. The present study was to detect the effect of bFGF on the activities of mitogen-activated protein kinase (MAPK) signaling pathway in rat intestine after I/R injury, and to investigate the protective mechanisms of bFGF on intestinal ischemia injury.

Preparation of collagen-based materials for wound dressing.

Objective: To describe the methods which were used to develop collagen-based materials for wound dressing.

Methods: Fresh frozen bovine tendon was treated with 0.05 mol/L acetic acid at pH 3.

Thermal injuries induce gene expression of endogenous c-fos, c-myc and bFGF in burned tissues.

Objective: To investigate the expression sequence and distribution characteristics of the protooncogenes c-fos, c-myc and endogenous basic fibroblast growth factor (bFGF) genes in burned tissues, and to explore the possible effects of changes in these genes' functions on wound healing.

Methods: Partial-thickness burns of 30% TBSA were established on backs of Wistar rats. In situ hybridization and histological methods were used to detect expression of c-fos, c-myc and bFGF genes in normal and burned tissue at 3 h, 6 h, 1 d, 3 d, 7 d and 14 d postburn.

[Characteristics of bFGF and TGF-beta expression in dermal chronic ulcers and hypertrophic scars and their effects on tissue repair].

Objective: To localize the distribution of basic fibroblast growth factor (bFGF) and transforming growth factor-beta(TGF-beta) in tissues from dermal chronic ulcer and hypertrophic scar and to explore their effects on tissue repair.

Methods: Twenty-one cases were detected to localize the distribution of bFGF and TGF-beta, among them, there were 8 cases with dermal chronic ulcers, 8 cases with hypertrophic scars, and 5 cases of normal skin.

Results: Positive signal of bFGF and TGF-beta could be found in normal skin, mainly in the keratinocytes.

Healing of chronic cutaneous wounds by topical treatment with basic fibroblast growth factor.

Objective: To evaluate the safety and efficacy of topical application of recombinant bovine basic fibroblast growth factor (rbFGF) on the healing of chronic cutaneous wounds.

Methods: Twenty-eight patients with thirty-three chronic cutaneous wounds resulting from trauma, diabetes mellitus, pressure sore and radiation injuries were enrolled in this prospective, open-label crossover trial. Prior to treatment with rbFGF, all wounds failed to heal with conventional therapies within 4 weeks.

[Epidermal growth factor stimulates tissue repair in skin through skin stem cell activation].

Objective: To explore the possible mechanisms of skin regeneration through the epidermal stem cells stimulated by epidermal growth factor (EGF).

Methods: At 8 and 14 days after treatment with EGF, the tissue specimens from 8 skin ulcered patients who were treated with EGF were used to evaluate the distribution and differentiation of epidermal stem cells. The expression of beta 1 integrin, keratin 19 (K19), keratin 14(K14) and keratin 10 (K10) in skin was detected with SP immunohistochemical methods.

Relationship between plasma D(-)-lactate and intestinal damage after severe injuries in rats.

Aim: To explore the kinetic changes in plasma D(-)-lactate and lipopolysaccharide (LPS) levels, and investigate whether D(-)-lactate could be used as a marker of intestinal injury in rats following gut ischemia/reperfusion, burn, and acute necrotizing pancreatitis (ANP).

Methods: Three models were developed in rats: (1)gut ischemia/reperfusion obtained by one hour of superior mesenteric artery occlusion followed by reperfusion; (2)severe burn injury created by 30% of total body surface area (TBSA) full-thickness scald burn; and (3)ANP induced by continuous inverse infusion of sodium taurocholate and trypsin into main pancreatic duct. Plasma levels of D(-)-lactate in systemic circulation and LPS in portal circulation were measured by enzymatic-spectrophotometric method and limulus amebocyte lysate (LAL) test kit, respectively.