2 results match your criteria: "CBRNE - Ricin"
Rapid Denaturing Organic Digestion Method for Targeted Protein Identification and Characterization.
Anal Chem
March 2021
Nottingham Trent University, Clifton Campus, College Drive, Nottingham NG11 8NS, U.K.
Article Synopsis
- Bottom-up mass spectrometry is used for high-specificity protein analysis, but traditional methods face issues like long digestion times and variability in results.
- A new 5-minute tryptic denaturing organic digestion (DOD) method has been validated as a faster alternative, showing improved performance in protein digestion compared to current methods like FASP and Flash Digest.
- The DOD method achieved excellent sequence coverage (55 to 100%) with better reproducibility, substantially reducing digestion time while maintaining or enhancing identification accuracy for various protein toxins.
Ricin Antibodies' Neutralizing Capacity against Different Ricin Isoforms and Cultivars.
Toxins (Basel)
January 2021
Paris-Saclay University, CEA, INRAE, Medicines and Healthcare Technologies Department (DMTS), SPI, 91191 Gif-sur-Yvette, France.
Article Synopsis
- Ricin is a highly toxic protein and potential biowarfare agent with no approved specific treatment available.
- Antibodies have been explored for protection, but their effectiveness may vary against different ricin isoforms (D and E) due to high specificity.
- This study found that certain antibodies have varied neutralizing effects depending on the ricin variant and that a combination of specific antibodies can significantly enhance protective outcomes in mouse models.