Real-world clinical multi-omics analyses reveal bifurcation of ER-independent and ER-dependent drug resistance to CDK4/6 inhibitors.

To better understand drug resistance mechanisms to CDK4/6 inhibitors and inform precision medicine, we analyze real-world multi-omics data from 400 HR+/HER2- metastatic breast cancer patients treated with CDK4/6 inhibitors plus endocrine therapies, including 200 pre-treatment and 227 post-progression samples. The prevalences of ESR1 and RB1 alterations significantly increase in post-progression samples. Integrative clustering analysis identifies three subgroups harboring different resistance mechanisms: ER driven, ER co-driven and ER independent.

Motif distribution and DNA methylation underlie distinct Cdx2 binding during development and homeostasis.

Transcription factors guide tissue development by binding to developmental stage-specific targets and establishing an appropriate enhancer landscape. In turn, DNA and chromatin modifications direct the genomic binding of transcription factors. However, how transcription factors navigate chromatin features to selectively bind a small subset of all the possible genomic target loci remains poorly understood.

Inflammatory disease progression shapes nanoparticle biomolecular corona-mediated immune activation profiles.

Polymeric nanoparticles (NPs) are promising tools used for immunomodulation and drug delivery in various disease contexts. The interaction between NP surfaces and plasma-resident biomolecules results in the formation of a biomolecular corona, which varies patient-to-patient and as a function of disease state. This study investigates how the progression of acute systemic inflammatory disease influences NP corona compositions and the corresponding effects on innate immune cell interactions, phenotypes, and cytokine responses.

Harnessing artificial intelligence for enhanced veterinary diagnostics: A look to quality assurance, Part II External validation.

Artificial intelligence (AI) is emerging as a valuable diagnostic tool in veterinary medicine, offering affordable and accessible tests that can match or even exceed the performance of medical professionals in similar tasks. Despite the promising outcomes of using AI systems (AIS) as highly accurate diagnostic tools, the field of quality assurance in AIS is still in its early stages. Our Part I manuscript focused on the development and technical validation of an AIS.

Effective interventions to prevent youth vaping behaviours: a rapid review.

Objective: To identify effective policies and non-policy interventions preventing youth vaping behaviour initiation and assess their effectiveness by the level of intrusiveness and subpopulations.

Design: This systematic rapid review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines.

Data Sources: Searches on MEDLINE and APA-PsycINFO for studies published between January 2019 and November 2023.

Short-acting beta agonist, antibiotics, oral corticosteroid and association with mortality and cardiopulmonary events in patients with COPD: a retrospective cohort study in Alberta, Canada.

Objective: The purpose of the study was to examine the association between short-acting beta agonist (SABA), antibiotic and oral corticosteroid (OCS) use and mortality and cardiopulmonary outcomes in chronic obstructive pulmonary disease (COPD).

Design: Retrospective cohort study using administrative health data from 1 April 2011 to 31 March 2020.

Setting: Alberta, Canada.

Leveraging community engagement to shape biomedical research priorities.

Community engagement is essential for shaping equitable biomedical research priorities, but it is often underutilized, especially for marginalized populations. To integrate community feedback from the public into research, herein we describe a collaborative pilot funded by the Chan Zuckerberg Initiative which pairs the University of California San Francisco (UCSF) with the Rafiki Coalition for Health and Wellness. Utilizing focus groups modeled on Research Prioritization by Affected Communities, participants identified themes that included mistrust in healthcare, representation gaps, and the need for culturally responsive research.

Efficacy and empathy of AI chatbots in answering frequently asked questions on oral oncology.

Objectives: Artificial intelligence chatbots have demonstrated feasibility and efficacy in improving health outcomes. In this study, responses from 5 different publicly available AI chatbots-Bing, GPT-3.5, GPT-4, Google Bard, and Claude-to frequently asked questions related to oral cancer were evaluated.

Relative efficacy of prehabilitation interventions and their components: systematic review with network and component network meta-analyses of randomised controlled trials.

Objective: To estimate the relative efficacy of individual and combinations of prehabilitation components (exercise, nutrition, cognitive, and psychosocial) on critical outcomes of postoperative complications, length of stay, health related quality of life, and physical recovery for adults who have received surgery.

Design: Systematic review with network and component network meta-analyses of randomised controlled trials.

Data Sources: Medline, Embase, PsycINFO, CINAHL, Cochrane Library, and Web of Science were initially searched 1 March 2022, and updated on 25 October 2023.

Changes in doxylamine-pyridoxine dispensation in Ontario following media attention: Time-series analysis.

Objective: To understand the possible association between media coverage and changes in the dispensation of doxylamine-pyridoxine in Canada.

Design: Cross-sectional time-series analysis using data from the IQVIA CompuScript database.

Setting: Ten Canadian provinces.

Reducing Quotation Errors in Scientific Manuscripts: A Novel Approach from the Global Andrology Forum.

Purpose: This study investigated 1) the frequency of quotation errors in multi-authored medical manuscripts in andrology, 2) analyzed common types of quotation errors and the methods used to rectify them, and 3) evaluated their impact on manuscript accuracy, credibility, and research conclusions.

Materials And Methods: Twelve manuscripts written by the Global Andrology Forum (GAF) members between 2023 and 2024 were randomly selected for this study. The manuscripts and "Quotation Verification Sheets" were analyzed by senior GAF researchers to detect the number and types of quotation errors.

Lightweight 3D-printed chitosan/MXene aerogels for advanced electromagnetic shielding, energy harvesting, and thermal management.

This study focuses on the fabrication of 3D-printed chitosan/TiCT-MXene aerogels with varying MXene concentrations (1, 2, 5, and 10 wt%) using the direct ink writing (DIW) method. The inks were freeze-dried to form aerogels, and FTIR and XRD analyses confirmed interactions between chitosan and MXene molecules, leading to increased spacing between MXene nanosheets. Rheological testing showed improved shear-thinning behavior, enhancing printability.

Rational design of a starch/whey protein isolate/caffeic acid ternary system to alleviate gel deterioration during freeze-thaw cycles.

To strengthen starch gel quality and improve the deterioration from freeze-thaw cycles, corn starch/whey protein isolate (WPI)/caffeic acid (CA) composite gels were rationally constructed in this study. The results showed that the introduction of WPI and CA significantly optimized the microstructure of the gels, an observation verified by SEM and CLSM. In addition, FT-IR and XRD analyses further revealed that the interaction mechanism within the composite gel was mainly due to the reinforcement of hydrogen bonds.

3D-printed sodium alginate/carbon nanotube/graphene porous scaffolds crosslinked with Ca for high-performance electromagnetic shielding and Joule heating.

High-performance green functional materials have garnered significant interest for electromagnetic interference (EMI) shielding applications, but creating customized, low-density, high-strength and high-efficiency biomass-based shielding materials remains challenging. In this study, lightweight Ca doped sodium alginate (SA) porous scaffolds with a carbon nanotube (CNT)/graphene (Gr) hybrid conductive network were fabricated via direct ink writing (DIW) 3D printing. The SA/CNT/Gr inks with unique rheological properties were formulated and architectures with arbitrarily customized structures could be freely constructed based on the printable inks.

Effect of crosslinker length in CNF foams for oil recovery and sustainable agriculture.

Chemically crosslinked foams possess good wet mechanical stability, and they are promising systems for applications in oil recovery, water treatment, energy storage, etc. However, reports on the effect of crosslinker length on the physical properties of the foam are scarce. Various cellulose nanofiber (CNF) foams (denoted as CPM) were prepared using different molecular weights dicarboxylated-PEG crosslinkers via the esterification reaction.

Synthesis of a novel starch-based emulsion gel with remarkable low-temperature stability via esterification, ozone-oxidation and ion induction.

A novel starch-based emulsion gel was designed via octenyl succinic anhydride (OSA) esterification, ozone oxidation, and ion (Ca) induction. The gel properties and low-temperature stability of emulsion gel with different oxidation time (0, 5, 10, 15, 25 min; OW-0, 5, 10, 15, 25) were systematically investigated. FTIR revealed that the oxidation of CC and -OH groups in OW-0 by ozone oxidation led to their cleavage into carbonyl groups, and than transformed to carboxyl groups.

Alginate-functionalized nanoceria as ion-responsive eye drop formulation to treat corneal abrasion.

In this study, we aimed to develop ion-responsive and biocompatible alginate-capped nanoceria (Ce-ALG) for β-1,3-glucan (i.e., wound healing agent) delivery and corneal abrasion (CA) treatment.

Bioinspired complex cellulose nanorod-architectures: A model for dual-responsive smart carriers.

The synergy between nanomaterials as solid supports and supramolecular concepts has resulted in nanomaterials with hierarchical structure and enhanced functionality. Herein, we developed and investigated innovative supramolecular functionalities arising from the synergy between organic moieties and the preexisting nanoscale soft material backbones. Based on these complex molecular nano-architectures, a new nanorod carbohydrate polymer carrier was designed with bifunctional hairy nanocellulose (BHNC) to reveal dual-responsive advanced drug delivery (ADD).

Cellulose-based hydrogel simultaneously possessing solar and evaporative cooling performances for energy-saving window and personal thermal management.

Thermochromic smart windows have been widely developed for building energy saving. However, most smart windows suffer from limited energy-saving performance, fixed phase transition temperature, and are not suitable for the temperature regulation needs of different application scenarios. Herein, a unique self-adaptive thermochromic hydrogel (HBPEC-PNA) with self-moisture-absorbing performance is reported that assembles solar energy cooling and evaporative heat dissipation.

The inverse electron demand diels-alder (IEDDA): A facile bioorthogonal click reaction for development of injectable polysaccharide-based hydrogels for biomedical applications.

The inverse electron demand Diels-Alder (IEDDA) cycloaddition between tetrazines and strained dienophiles is recognized as a fast and specific reaction. The integrating tetrazines and strained dienophiles onto the backbone of polysaccharides yield appropriate water-soluble precursors for IEDDA cycloaddition. Due to the high specificity of the IEDDA reaction and its outstanding cytocompatibility, a range of cargos (live cells, peptides and pharmaceuticals) can be effectively encapsulated in polysaccharide solutions throughout the hydrogel formation.

Synthesis and characterization of photo-cross-linkable quince seed-based hydrogels for soft tissue engineering applications.

The convenience, versatility, and biocompatibility of photocrosslinkable hydrogel precursors make them promising candidates for developing tissue engineering scaffolds. However, the current library of photosensitive materials is limited. This study reports, for the first time, the modification of quince seed mucilage (QS) with glycidyl methacrylate (GM), resulting in the synthesis of methacrylated QS (QSGM).