In vivo Tissue Evaluation Reveals Improvements in Explicit PDT Dosimetry.

Progress is needed before explicit photodynamic therapy (PDT) dosimetry can treat peritoneal carcinomatosis and yet spare all healthy tissue. A report by Cengel et al. in this issue of Photochemistry & Photobiology on tissue evaluation in a canine model may bring that goal a step closer and may even be dogma-changing.

Soil toxicants that potentially affect children's health.

Soil pollution is a global phenomenon, and children are uniquely susceptible to the wide range of toxicants that persist in topsoil. Given their increased exposure through mouthing activities, increased body surface area, likelihood of breathing air closer to soil, and immature immune and elimination systems, it is essential to understand the mechanisms of children's exposure and the potential health effects of toxicants found in soil. Here we describe the sources and toxicological profiles of a range of inorganic and organic soil contaminants, including arsenic (As), cadmium (Cd), lead (Pb), mercury (Hg), benzene, toluene, ethylbenzene and xylenes, chlorinated dibenzo-p-dioxins, polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), per and polyfluoroalkyl substances (PFAS), as well as agricultural and domestic sources of pollution.

Prenatal Choline Supplementation during High-Fat Feeding Improves Long-Term Blood Glucose Control in Male Mouse Offspring.

Maternal obesity increases the risk of metabolic dysregulation in rodent offspring, especially when offspring are exposed to a high-fat (HF), obesogenic diet later in life. We previously demonstrated that maternal choline supplementation (MCS) in HF-fed mouse dams during gestation prevents fetal overgrowth and excess adiposity. In this study, we examined the long-term metabolic influence of MCS.

Reconstruction and analysis of a carbon-core metabolic network for Dunaliella salina.

Background: The green microalga Dunaliella salina accumulates a high proportion of β-carotene during abiotic stress conditions. To better understand the intracellular flux distribution leading to carotenoid accumulation, this work aimed at reconstructing a carbon core metabolic network for D. salina CCAP 19/18 based on the recently published nuclear genome and its validation with experimental observations and literature data.

Pain-Evoked Reorganization in Functional Brain Networks.

Recent studies indicate that a significant reorganization of cerebral networks may occur in patients with chronic pain, but how immediate pain experience influences the organization of large-scale functional networks is not yet well characterized. To investigate this question, we used functional magnetic resonance imaging in 106 participants experiencing both noxious and innocuous heat. Painful stimulation caused network-level reorganization of cerebral connectivity that differed substantially from organization during innocuous stimulation and standard resting-state networks.

Static Solid Relaxation Ordered Spectroscopy: SS-ROSY.

A two-dimensional pulse sequence is introduced for correlating nuclear magnetic resonance anisotropic chemical shifts to a relaxation time (e.g., T) in solids under static conditions.

Comparative Study of Water-Mediated Interactions between Hydrophilic and Hydrophobic Nanoscale Surfaces.

Self-assembly processes in aqueous solutions, such as protein folding and nanoparticle aggregation, are driven by water-mediated interactions (WMIs). The most common of such interactions are the attractive forces between hydrophobic units. While numerous studies have focused on hydrophobic interactions, WMIs between hydrophilic moieties and pairs of hydrophilic-hydrophobic surfaces have received much less attention.

Synthesis and Characterization of Ru(II) Complexes with π-Expansive Imidazophen Ligands for the Photokilling of Human Melanoma Cells.

Ru(II) complexes were synthesized with π-expanding (phenyl, fluorenyl, phenanthrenyl, naphthalen-1-yl, naphthalene-2-yl, anthryl and pyrenyl groups) attached at a 1H-imidazo[4,5-f][1,10]phenanthroline ligand and 4,4'-dimethyl-2,2'-bipyridine (4,4'-dmb) coligands. These Ru(II) complexes were characterized by 1D and 2D NMR, and mass spectroscopy, and studied for visible light and dark toxicity to human malignant melanoma SK-MEL-28 cells. In the SK-MEL-28 cells, the Ru(II) complexes are highly phototoxic (EC  = 0.

The concurrent decline of soil lead and children's blood lead in New Orleans.

Lead (Pb) is extremely toxic and a major cause of chronic diseases worldwide. Pb is associated with health disparities, particularly within low-income populations. In biological systems, Pb mimics calcium and, among other effects, interrupts cell signaling.

An Amyloid Core Sequence in the Major Candida albicans Adhesin Als1p Mediates Cell-Cell Adhesion.

The human fungal commensal can become a serious opportunistic pathogen in immunocompromised hosts. The cell adhesion protein Als1p is a highly expressed member of a large family of paralogous adhesins. Als1p can mediate binding to epithelial and endothelial cells, is upregulated in infections, and is important for biofilm formation.

Perturbation potentials to overcome order/disorder transitions in alchemical binding free energy calculations.

We investigate the role of order/disorder transitions in alchemical simulations of protein-ligand absolute binding free energies. We show, in the context of a potential of mean force description, that for a benchmarking system (the complex of the L99A mutant of T4 lysozyme with 3-iodotoluene) and for a more challenging system relevant for medicinal applications (the complex of the farnesoid X receptor with inhibitor 26 from a recent D3R challenge) that order/disorder transitions can significantly hamper Hamiltonian replica exchange sampling efficiency and slow down the rate of equilibration of binding free energy estimates. We further show that our analytical model of alchemical binding combined with the formalism developed by Straub et al.

Choline: Exploring the Growing Science on Its Benefits for Moms and Babies.

The importance of ensuring adequate choline intakes during pregnancy is increasingly recognized. Choline is critical for a number of physiological processes during the prenatal period with roles in membrane biosynthesis and tissue expansion, neurotransmission and brain development, and methyl group donation and gene expression. Studies in animals and humans have shown that supplementing the maternal diet with additional choline improves several pregnancy outcomes and protects against certain neural and metabolic insults.

Measuring Adaptive Control in Conflict Tasks.

The past two decades have witnessed an explosion of interest in the cognitive and neural mechanisms of adaptive control processes that operate in selective attention tasks. This has spawned not only a large empirical literature and several theories but also the recurring identification of potential confounds and corresponding adjustments in task design to create confound-minimized metrics of adaptive control. The resulting complexity of this literature can be difficult to navigate for new researchers entering the field, leading to suboptimal study designs.

The generalized Boltzmann distribution is the only distribution in which the Gibbs-Shannon entropy equals the thermodynamic entropy.

We show that the generalized Boltzmann distribution is the only distribution for which the Gibbs-Shannon entropy equals the thermodynamic entropy. This result means that the thermodynamic entropy and the Gibbs-Shannon entropy are not generally equal, but rather the equality holds only in the special case where a system is in equilibrium with a reservoir.

Transcranial Direct Current Stimulation over the Prefrontal Cortex Alters Encoding and Judgments of Learning Based on Fluency.

Past research has shown that judgments of learning (JOLs), subjective confidence judgments made at study about later memorability, are inferential in nature and based on cues available during encoding. Participants tend to use fluency as a cue and give higher JOLs to more fluently encoded items, despite having better recognition memory for disfluently encoded items, which leads to poor JOL accuracy. Research has implicated the dorsolateral prefrontal cortex (DLPFC) and anterior prefrontal cortex (aPFC) in JOL and encoding processes, but no studies to date have tested how the roles of these regions vary with the information on which JOLs are based.

Factors associated with outpatient follow-up after a pediatric inpatient stay at a community hospital.

Background And Objectives: Pediatric settings often use a patient-centered medical home model in caring for patients in the outpatient setting and for attempting to connect inpatient care with outpatient follow-up. This medical home model has proven to be beneficial in many aspects of patient care, but there needs to be a good transition between inpatient and outpatient services. Our goal in this study is to determine the association of particular variables with adherence to outpatient follow-up after a general inpatient stay, in the pediatric population.

Glass polymorphism in TIP4P/2005 water: A description based on the potential energy landscape formalism.

The potential energy landscape (PEL) formalism is a statistical mechanical approach to describe supercooled liquids and glasses. Here, we use the PEL formalism to study the pressure-induced transformations between low-density amorphous ice (LDA) and high-density amorphous ice (HDA) using computer simulations of the TIP4P/2005 molecular model of water. We find that the properties of the PEL sampled by the system during the LDA-HDA transformation exhibit anomalous behavior.

State variables for glasses: The case of amorphous ice.

Glasses are out-of-equilibrium systems whose state cannot be uniquely defined by the usual set of equilibrium state variables. Here, we seek to identify an expanded set of variables that uniquely define the state of a glass. The potential energy landscape (PEL) formalism is a useful approach within statistical mechanics to describe supercooled liquids and glasses.

A Field Procedure To Screen Soil for Hazardous Lead.

Soils retain lead contamination from possible sources such as mining, smelting, battery recycling, waste incineration, leaded gasoline, and crumbling paint. Such contamination is often concentrated in toxic hot spots that need to be identified locally. To address this need, a simple field procedure was designed to screen soil for hazardous Pb for use by the general public.

Household Support for Physical Activity in Adolescent Girls Living in Primarily Low Socioeconomic Status Neighborhoods.

The purpose of this study was to examine the association between perception of household support and physical activity levels of adolescent girls living in primarily low socioeconomic status (SES) neighborhoods. The sample consisted of thirty-six adolescent girls (N=36; 60% non-Hispanic Black; mean age of 14.6 ± 1.

Green Infrastructure Design Influences Communities of Urban Soil Bacteria.

The importance of natural ecosystem processes is often overlooked in urban areas. Green Infrastructure (GI) features have been constructed in urban areas as elements to capture and treat excess urban runoff while providing a range of ancillary benefits, e.g.

Bullying Perpetration and Victimization among Adolescents with Overweight and Obesity in a Nationally Representative Sample.

Adolescents with obesity are more likely to experience bullying in comparison to their healthy weight peers. However, it is unclear whether adolescents with obesity are more likely to perpetuate bullying or be both, a bully perpetrator and a bully victim. The purpose of this analysis was to examine differences in bully perpetration, victimization, and both (perpetration and victimization) by BMI classification in a nationally representative sample of adolescents.

The serial blocking effect: a testbed for the neural mechanisms of temporal-difference learning.

Temporal-difference (TD) learning models afford the neuroscientist a theory-driven roadmap in the quest for the neural mechanisms of reinforcement learning. The application of these models to understanding the role of phasic midbrain dopaminergic responses in reward prediction learning constitutes one of the greatest success stories in behavioural and cognitive neuroscience. Critically, the classic learning paradigms associated with TD are poorly suited to cast light on its neural implementation, thus hampering progress.