Detection of clinically relevant variants in the gene below 10% allelic frequency: A multicenter study by ERIC, the European Research Initiative on CLL.

In chronic lymphocytic leukemia, the reliability of next-generation sequencing (NGS) to detect variants ≤10% allelic frequency (low-VAF) is debated. We tested the ability to detect 23 such variants in 41 different laboratories using their NGS method of choice. The sensitivity was 85.

In Vitro Assessment of Chitosan-PEG Hydrogels Enriched with MSCs-Exosomes for Enhancing Wound Healing.

Regenerating skin tissue remains a major challenge in medical science, especially due to the risk of scarring and prolonged healing, which becomes even more complicated in people with diabetes. Recent advancements have led to the creation of therapeutic dressings incorporating drug-delivery systems to tackle these issues. Exosomes (Exos) derived from mesenchymal stem cells (MSCs) have gained significant attention for mediating therapy without directly using cells, thanks to their natural anti-inflammatory and tissue repair properties mirroring those of MSCs.

Renewable energy transition in Europe in the context of renewable energy transition processes in the world. A review.

Both the global and European energy sectors have been undergoing a deep transition for several years, associated with a reduction in the overall share of conventional coal-based energy in favor of new technologies, especially energy from renewable sources (RES). This transition is moving from centralized production towards distributed technologies and from providing only energy to end users towards combining innovative products and services with it. Electricity consumers are becoming prosumers connected to the grid and generating an increasing amount of energy.

Amide conjugates of the jasmonate precursor cis-(+)-12-oxo-phytodienoic acid regulate its homeostasis during plant stress responses.

Jasmonates are a family of oxylipin phytohormones regulating plant development and growth and mediating "defense versus growth" responses. The upstream JA biosynthetic precursor cis-(+)-12-oxo-phytodienoic acid (cis-OPDA) acts independently of CORONATIVE INSENSITIVE 1-mediated JA signaling in several stress-induced and developmental processes. However, its perception and metabolism are only partially understood.

Comprehensive study of rapid capacity fade in prismatic Li-ion cells with flexible packaging.

Prismatic lithium-ion batteries (LIBs) are considered promising electric energy sources in electromobility applications due to their efficient space utilization. However, their sensitivity to external and internal influences and reduced durability lead to inflation risk and potential explosions throughout their lifecycle. These critical processes are strongly influenced by the inner construction of the cell, especially concerning the coating and mechanical fixation.

Adjuvant immunotherapy in patients with resected gastric and oesophagogastric junction cancer following preoperative chemotherapy with high risk for recurrence (ypN+ and/or R1): European Organisation of Research and Treatment of Cancer (EORTC) 1707 VESTIGE study.

Background: Patients with gastro-oesophageal adenocarcinoma with tumour-positive lymph nodes (ypN+) or positive surgical margins (R1) following neoadjuvant chemotherapy and resection are at high risk of recurrence. Adjuvant nivolumab is effective in oesophageal/oesophagogastric junction cancer and residual pathological disease following chemoradiation and surgery. Immune checkpoint inhibition has shown efficacy in advanced gastro-oesophageal cancer.

PETG as an Alternative Material for the Production of Drone Spare Parts.

Material selection is the main challenge in the drone industry. In this study, hardness, abrasive wear, impact resistance, tensile strength, and durability (frost resistance and accelerated ageing) were identified as important characteristics of drone materials. The additive manufacturing technology was used to produce the drone leg specimens and prototype.

A wireless W-band 3D-printed temperature sensor based on a three-dimensional photonic crystal operating beyond 1000 C.

In addressing sensing in harsh and dynamic environments, there are no available millimeter-wave chipless and wireless sensors capable of continuous operation at extremely high temperatures. Here we present a fully dielectric wireless temperature sensor capable of operating beyond 1000 C. The sensor uses high-Q cavities embedded within a three-dimensional photonic crystal resonating at 83.

Factors shaping home ranges of Eurasian lynx (Lynx lynx) in the Western Carpathians.

Understanding how large carnivores utilize space is crucial for management planning in human-dominated landscape and enhances the accuracy of population size estimates. However, Eurasian lynx display a large inter-population variation in the size of home ranges across their European range which makes extrapolation to broader areas of a species distribution problematic. This study evaluates variations in home range size for 35 Eurasian lynx in the Western Carpathians during 2011-2022 based on GPS telemetry and explains how intrinsic and environmental factors shape lynx spatial behaviour when facing anthropogenic pressure.

Survey on 5G Physical Layer Security Threats and Countermeasures.

With the expansion of wireless mobile networks into both the daily lives of individuals as well as into the widely developing market of connected devices, communication is an increasingly attractive target for attackers. As the complexity of mobile cellular systems grows and the respective countermeasures are implemented to secure data transmissions, the attacks have become increasingly sophisticated on the one hand, but at the same time the system complexity can open up expanded opportunities for security and privacy breaches. After an in-depth summary of possible entry points to attacks to mobile networks, this paper first briefly reviews the basic principles of the physical layer implementation of 4G/5G systems, then gives an overview of possible attacks from a physical layer perspective.

Phosphoric acid salts of amino acids as a source of oligopeptides on the early Earth.

Because of their unique proton-conductivity, chains of phosphoric acid molecules are excellent proton-transfer catalysts. Here we demonstrate that this property could have been exploited for the prebiotic synthesis of the first oligopeptide sequences on our planet. Our results suggest that drying highly diluted solutions containing amino acids (like glycine, histidine and arginine) and phosphates in comparable concentrations at elevated temperatures (ca.

Light-Controlled Electric Stimulation with Organic Electrolytic Photocapacitors Achieves Complex Neuronal Network Activation: Semi-Chronic Study in Cortical Cell Culture and Rat Model.

Neurostimulation employing photoactive organic semiconductors offers an appealing alternative to conventional techniques, enabling targeted action and wireless control through light. In this study, organic electrolytic photocapacitors (OEPC) are employed to investigate the effects of light-controlled electric stimulation on neuronal networks in vitro and in vivo. The interactions between the devices and biological systems are characterized.

Short-Term Entropy of Signal Energy Used for Effective Detecting of Weak Gunshots in Noisy Environments.

Conventional gunshot detection systems can quickly and reliably detect gunshots in the area where the acoustic sensors are placed. This paper presents the detection of weak hunting gunshots using the short-term entropy of signal energy computed from acoustic signals in an open natural environment. Our research in this field was primarily aimed at detecting gunshots fired at close range with the usual acoustic intensity to protect wild elephants from poachers.

Above room temperature spin crossover in mononuclear iron(II) complexes featuring pyridyl-benzimidazole bidentate ligands adorned with aliphatic chains.

Two bidentate ligands (L1 = 1-pentyl-2-(pyridin-2-yl)-1-benzimidazole and L2 = 1-heptyl-2-(pyridin-2-yl)-1-benzimidazole) were employed for the synthesis of five mononuclear Fe(II) coordination compounds 1-5 containing perchlorate, tetrafluoroborate and triflate counterions. Single-crystal X-ray diffraction analysis confirmed the expected molecular structures of all the reported compounds, revealing a moderately distorted octahedral geometry of {FeN} coordination chromophores. All five compounds exhibit thermal spin crossover with temperatures allocated above 400 K.

Magnetic anisotropy and slow relaxation of magnetisation in double salts containing four- and six-coordinate cobalt(II) complex ions.

Four novel Co(II) coordination compounds 1-4 of the general formula [Co(L)][Co(NCY)]·CHCN (where L are tridentate ligands L1 = 2,6-bis(1-hexyl-1-benzimidazol-2-yl)pyridine for 1 and 2; L2 = 2,6-bis(1-octyl-1-benzimidazol-2-yl)pyridine for 3; L3 = 2,6-bis(1-dodecyl-1-benzimidazol-2-yl)pyridine for 4, Y = O for 1, 3, and 4 and Y = S for 2; = 0 for 1 and 3, = 0.5 for 2 and = 2 for 4) were prepared and characterised. The molecular structures of all four compounds consist of the hexacoordinate complex cation [Co(L)] and tetracoordinate complex anion [Co(NCY)], with distorted octahedral and tetrahedral symmetry of coordination polyhedra, respectively.

Identifying pesticides of high concern for ecosystem, plant, animal, and human health: A comprehensive field study across Europe and Argentina.

The widespread and excessive use of pesticides in modern agricultural practices has caused pesticide contamination of the environment, animals, and humans, with confirmed serious health consequences. This study aimed to identify the 20 most critical substances based on an analysis of detection frequency (DF) and median concentrations (MC) across environmental and biological matrices. A sampling campaign was conducted across 10 case study sites in Europe and 1 in Argentina, each encompassing conventional and organic farming systems.

Photoswitchable hydrazones with pyridine-based rotors and halogen substituents.

The ,-photoisomerization of pyridine-based hydrazone switches is typically suppressed due to the presence of pyridine-based rotors. The crystal structures of studied compounds were investigated using theoretical methods combining DFT and QT-AIM calculations to unveil the nature and properties of the intramolecular hydrogen bonding. In this study, we introduced a new series of pyridine-based hydrazones anchored with -halogen substituents (2-X) and investigated their photoswitching abilities using H NMR and UV-Vis spectroscopy.

Biotransformation of ferulic acid into vanillyl alcohol and vanillic acid employing thermophilic bacterium Caldimonas thermodepolymerans.

Caldimonas thermodepolymerans, a Gram-negative, moderately thermophilic bacterium, exhibits a remarkable biotechnological potential. Given the presence of genes in its genome dedicated to the metabolization of ferulic acid (FA), this study aimed to explore the bacterium's capability for biotransforming FA into high-value metabolites. The results unequivocally demonstrate the bacterium's proficiency in the efficient and rapid conversion of FA into vanillyl alcohol (VOH) and vanillic acid (VA).

Levamisole Based Co(II) Single-Ion Magnet.

A new Co(II) complex, [Co(NCS)(L)] (1) has been synthesized based on levamisole (L) as a new ligand. Single-crystal X-ray diffraction analyses confirm that the Co(II) ion is having a distorted tetrahedral coordination geometry in the complex. Notably strong intramolecular S⋅⋅⋅S and S⋅⋅⋅N interactions has been confirmed by employing Quantum Theory of Atoms in Molecules (QTAIM).

Growth status and age at peak height velocity among youth participants in several sports: the Cracow longitudinal study.

Background: Studies addressing age at peak height velocity (PHV) in longitudinal samples of participants in sports are relatively limited.

Purpose: To compare the growth status and estimated ages at PHV of longitudinal samples girls and boys active in sport with peers not active in sport, and to compare estimated ages at PHV among longitudinal samples of Polish youth active in sport.

Methods: Records from the Cracow Longitudinal Study, which measured youth annually from 8 to 18 years, were screened to identify individuals regularly active in sport.

Aberrant microbiomes are associated with increased antibiotic resistance gene load in hybrid mice.

Antibiotic resistance is a priority public health problem resulting from eco-evolutionary dynamics within microbial communities and their interaction at a mammalian host interface or geographical scale. The links between mammalian host genetics, bacterial gut community, and antimicrobial resistance gene (ARG) content must be better understood in natural populations inhabiting heterogeneous environments. Hybridization, the interbreeding of genetically divergent populations, influences different components of the gut microbial communities.

Sphere-shaped ZnO photocatalyst synthesis for enhanced degradation of the Quinolone antibiotic, Ofloxacin, under UV irradiation.

The sphere-shaped zinc oxide (ZnO) photocatalyst was synthesized by the homogeneous precipitation method, using Zn(CHCOO)·2HO as a zinc precursor and NHOH as a precipitating agent. The morphology and crystal structure of the prepared ZnO sample were studied by XRD, SEM, FT-IR, XPS, zeta potential measurements, and a low-temperature nitrogen adsorption-desorption technique. The optical characteristics of ZnO were determined by UV - Vis diffuse reflectance spectroscopy.