Characterisation of periorbital mechanical allodynia in the reserpine-induced fibromyalgia model in mice: The role of the Schwann cell TRPA1/NOX1 signalling pathway.

Fibromyalgia (FM) is a complex and multifaceted condition characterized by a range of clinical symptoms, including widespread pain and a strong association with migraine headaches. Recent findings have underscored the role of oxidative stress and transient receptor potential ankyrin 1 (TRPA1) channel in migraine and FM. However, the precise mechanisms underlying the comorbidity between migraine and FM are unclear.

A systematic review of the genus Tyzzeria Allen, 1936 (Chromista: Apicomplexa), including the molecular phylogenetic position of Tyzzeria parvula (Kotlán, 1933) Klimeš, 1963.

Tyzzeria Allen, 1936 is a coccidian genus traditionally associated with Anseriformes, although there are descriptions of Tyzzeria spp. from junglefowls, snakes, skinks and mice. In this sense, the current study performs a taxonomic review of Tyzzeria spp.

Interaction of normelinonine F and related N-methyl-β-carbolines derivatives with bovine serum albumin. Spectroscopic profiles, multivariate analysis and theoretical calculations.

β-carbolines (βCs) represent a large family of bioactive alkaloids, including norharmane and normelinonine F, known for their diverse pharmacological activities. The effects of these alkaloids may depend, among other factors, on their delivery, accumulation in different subcellular compartments, and interactions with biomacromolecules such as serum albumins. In this study, we investigated the pH dependence of the interactions between bovine serum albumin (BSA) and four βCs (norharmane, normelinonine F, and their corresponding N(9)-methyl derivatives) using UV-vis and fluorescence spectroscopy, combined with multivariate analysis and molecular docking.

Microfibrillated cellulose-based colorimetric sensor strips for detecting total iron in water.

Microfibrillated cellulose (MFC), a sustainable material derived from biomass, stands out as an environmentally friendly alternative for developing chemical sensors owing to its advantageous properties, including high porosity, surface area, and available surface functional groups. Herein, we propose a simple and low-cost strategy for developing cellulose-based strips for the colorimetric detection of total iron in water. The strips were prepared by functionalizing MFC casting membranes with 1-(2-Thiazolylazo)-2-naphthol (TAN), which was then characterized by structural and morphological techniques.

Probing the interaction of mannose-binding lectin with healthy and sickle cell anemia red blood cells and its role in cellular biomechanics.

Mannose-binding lectin (MBL) is an important glycoprotein of the human innate immune system. Furthermore, individuals with sickle cell anemia (SCA) and MBL deficiency seem more susceptible to vaso-occlusive crises, suggesting an MBL role on HbSS red blood cells (RBCs). This study investigated the interaction of MBL with HbA (healthy) and HbSS RBCs using optical tweezers (OT) and atomic force microscopy (AFM).

Genome assemblies and other genomic tools for understanding insect adaptation.

Insects, the most diverse group of animals, exhibit remarkable adaptability, playing both crucial and problematic roles in ecosystems. Recent advancements in genomic technologies, such as high-throughput sequencing, have provided unprecedented insights into the genetic foundations of insect adaptation. This review explores key methodologies, including de novo and reference-guided genome assemblies, and highlights cutting-edge technologies like second and third-generation sequencing, and hybrid techniques.

Immune regulation of host energy metabolism and periodicity of malaria parasites.

The synchronization of parasites as they replicate within red blood cells of their vertebrate host remains largely unexplored. Understanding this synchronization could reveal how parasites optimize their lifecycle to maximize transmission, evade the immune response and maximize energy acquisition. Rhythmic replication fulfils some criteria of an endogenous oscillator with time of day cues potentially provided by temperature, oxygen levels, hormones and/or nutrient availability.

A systematic review of the molecular mechanisms related to anthelmintic resistance in Haemonchus contortus: A contemporary narrative.

Haemonchus contortus is a gastrointestinal parasite that affects ruminants (cattle, sheep, etc.), having a significant welfare impact worldwide. The rise of anthelmintic resistance poses a growing challenge to adequate control, compromising the success of treatments.

Impact of mandibular overdentures on maxillary resorption and its association with masticatory function: A prospective longitudinal study with 3 years of follow-up.

Objective: This longitudinal clinical study monitored annually the maxillary and mandibular bone remodeling and masticatory function in complete denture (CD) wearers rehabilitated with implant-retained mandibular overdentures (MO) over three years and combined radiographic and masticatory function data to assess the correlation between bone remodeling and masticatory function.

Design: Thirty-nine MO wearers were monitored annually to assess changes in: i) residual ridge in the anterior and posterior maxillary region; ii) posterior height and posterior area index (PAI) in the mandible; and iii) masticatory function. Bone remodeling was measured through linear and angular measurements using panoramic radiographs.

Effect of caloric restriction with probiotic supplementation on body composition, quality of life, and psychobiological factors of obese men: A randomized, double-blinded placebo-controlled clinical trial.

Background & Aims: Obesity is a chronic disease characterized by an excess of fat mass. It is accompanied by a low-grade chronic systemic inflammation state that leads to numerous health disorders. To counteract this scenario, dietary-derived caloric restriction (CR) is the principal intervention for weight loss.

Intra- and inter-rater reliability, standard error of measurement, and minimal detectable change of the cranio-cervical flexion test in individuals with non-specific chronic neck pain.

Background: The cranio-cervical flexion test (CCFT) is used in clinical practice to measure the activation of deep cervical flexor muscles. However, the reliability of the test has not been conducted on an adequate sample size, specifically in individuals with non-specific chronic neck pain (CNP).

Objective: The aim of the present study is to investigate the intra- and inter-rater reliability, standard error of measurement (SEM), and minimal detectable change (MDC) of the CCFT in individuals with non-specific CNP.

3D printed skin dressings manufactured with spongin-like collagen from marine sponges: physicochemical properties and in vitro biological analysis.

Innovative skin dressings are needed for an effective skin wound treatment. Here in, it was used spongin-like collagen (SC) from marine sponge Chondrilla caribensis as a promising biomaterial, sodium alginate as matrix and, 3D printing technique to manufacture skin dressings in three concentrations (C1, C2, C3). The physicochemical, morphological and biological (in vitro) results were investigated.

Effects of the physical form of starter feed on the intake, performance, and health of female Holstein calves.

Available literature on the effect of various physical forms of starter feed (PFSF) on calf performance is conflicting. Thus, this study aimed to investigate the effect of the PFSF on feed intake, growth performance, blood metabolites, and the health of dairy calves. Twenty-four female Holstein calves (5-d-old; 40.

Photoinactivation of sulfate-reducing bacteria using 1,9-dimethyl-methylene blue - DMMB and laser light.

Annually, the oil and gas industry faces equipment losses and product quality degradation due to the presence of sulfate-reducing bacteria (SRB). Given the negative impact of SRB, this study evaluates the use of photoinactivation (PI) with zinc chloride double salt of 1,9-Dimethyl-Methylene Blue (DMMB) as a photosensitizer (PS) in varying concentrations and combined with Laser light at different exposures in an SRB consortium. For culture growth, a modified Postgate C medium (without ferrous sulfate) was used, and cell quantification was performed on 100 μL aliquots of the consortium, read on a spectrophotometer (λ600 nm) in an oxygen- and light-free environment at room temperature.

Predicted environmental concentration (PEC), environmental risk assessment (ERA) and prioritization of antiretroviral drugs (ARVs) in seawater from Guarujá (Brazilian coastal zone).

The antiretroviral therapy program's success in managing the human immunodeficiency virus (HIV) has inadvertently led to the release of antiretrovirals (ARVs) into worldwide aquatic ecosystems. However, few studies investigated the risks of ARV loadings that flow continuously to the marine waters of South America (such as Brazil). Against this backdrop, the aims of this study were: (i) to estimate the Predicted Environmental Concentration (PEC) of thirteen ARVs worldwide used in HIV treatment, and which are frequently disposed of in the marine aquatic ecosystems of Guarujá, São Paulo coastline, Brazil.

Exploratory metabolomic profiling of plasma and urine in patients with mucopolysaccharidosis type II (Hunter syndrome): A pilot study.

Mucopolysaccharidosis type II (MPS II), also known as Hunter syndrome, is an X-linked lysosomal storage disorder. It results from a deficiency of the enzyme iduronate-2-sulfatase (I2S), leading to the accumulation of glycosaminoglycans (GAGs) in various tissues and organs. Clinical manifestations include skeletal abnormalities, facial coarsening, organ enlargement, and developmental delays.

Early ultrastructural damage in retina and optic nerve following intraocular pressure elevation.

Elevated intraocular pressure (IOP) is a significant risk factor for glaucoma, causing structural and functional damage to the eye. Increased IOP compromises the metabolic and structural integrity of retinal ganglion cell (RGC) axons, leading to progressive degeneration and influencing the ocular immune response. This study investigated early cellular and molecular changes in the retina and optic nerve (ON) following ocular hypertension (OHT).

Epidemiology, Ventilation Management, and Outcomes in Invasively Ventilated Coronavirus Disease 2019 Patients: An Analysis of Four Observational Studies in Four Countries on Two Continents.

Epidemiology, ventilator management, and outcomes in patients with acute respiratory distress syndrome (ARDS) because of coronavirus disease 2019 (COVID-19) have been described extensively but have never been compared between countries. We performed an individual patient data analysis of four observational studies to compare epidemiology, ventilator management, and outcomes. We used propensity score weighting to control for confounding factors.

Small and Versatile Cyclotides as Anti-infective Agents.

Plants provide an abundant source of potential therapeutic agents, including a diverse array of compounds, such as cyclotides, which are peptides known for their antimicrobial activity. Cyclotides are multifaceted molecules with a wide range of biological activities. Their unique topology forms a head-to-tail cyclic structure reinforced by a cysteine knot, which confers chemical and thermal stability.

Wearable Smartphone-Based Multisensory Feedback System for Torso Posture Correction: Iterative Design and Within-Subjects Study.

Background: The prevalence of stroke is high in both males and females, and it rises with age. Stroke often leads to sensor and motor issues, such as hemiparesis affecting one side of the body. Poststroke patients require torso stabilization exercises, but maintaining proper posture can be challenging due to their condition.

Rock strength controls erosion in tectonically dead landscapes.

Interactions among tectonics, climate, and lithology shape the Earth's surface. In regions dominated by tectonic quiescence and climate stability, the role of rock strength related to lithology, and its role in landscape evolution, can be most clearly perceived. We leverage these qualities in a unique field site by integrating topographic data, erosion rates, and a large suite of rock strength measurements to quantify the relationship between bedrock strength and erosion rates along a 200-km section of the southeast coast of Brazil where climatic and tectonic variability are minimal.

A 4-year follow-up of the need for orthodontic treatment using the Dental Aesthetic Index-DAI: an exploratory analysis.

This study aim was to evaluate the need for orthodontic treatment of mixed to permanent dentition using the Dental Aesthetic Index (DAI) in a 4-year follow-up. A longitudinal study was conducted with 353 children in the stages from mixed (T1) to permanent (T2) dentition. The need for orthodontic treatment was assessed using the DAI categorized into: DAI 1 (absence of malocclusion and orthodontic treatment need; DAI ≤ 25); DAI 2 (malocclusion is defined and elective orthodontic treatment is needed; DAI = 26 to 30); DAI 3 (severe malocclusion and a desirable orthodontic treatment need; DAI = 31 to 35) and DAI 4 (severe malocclusion and a mandatory orthodontic treatment need; DAI ≥ 36).

Surface roughness of composite resins subjected to brushing with whitening toothpastes: an in vitro study.

The emergence of toothpastes containing different abrasive and whitening substances has been a constant concern among dental professionals. The aim of the present study was to perform an in vitro assessment of the surface topography of nanoparticle composite resins subjected to simulated brushing with dentifrices. Test samples were prepared with Filtek Universal (3M ESPE), Filtek Bulkfill (3M ESPE) and Z350 (3M ESPE), with 24 samples per resin.

Brazilian version of the OHIP 14 Periodontal Disease Questionnaire: cross cultural adaptation and validation.

Although it is recognized that periodontal disease negatively impacts quality of life, there is no validated instrument to assess this impact in Brazil. This study aimed to translate, cross-culturally adapt, and validate the OHIP 14 PD (Oral Health Impact Profile Applied to Periodontal Diseases) for application among Brazilian patients. The original instrument was translated and validated into Brazilian Portuguese in a cross-sectional study with 110 participants recruited from a Dental School clinic.