Sustainable extraction of phytoestrogens from soybean and okara using green solvents.

Soy extract waste, okara, is a rich source of bioactive compounds such as isoflavones, which are phytoestrogens with potential health benefits. To develop a green approach to recovering these compounds and valorizing okara, a study was developed to screen variables for the extraction of isoflavones from okara and soybean (for comparison) using Deep Eutectic Solvents (DES) composed with choline chloride ([Ch]Cl) and acetic acid (AA) ([Ch]Cl: AA, 1:2). A fractional design (2) was used to evaluate variables in the extraction of isoflavones, followed by a Central Composite Rotatable Design (CCRD).

The intricate relationship between SUMOylation and gliomas: a review with a perspective on natural compounds.

Gliomas are tumours that affect the nervous system, with glioblastoma, also known as grade IV astrocytoma, being the most aggressive type, associated with poor prognosis. Glioblastoma is characterised by its highly invasive nature, rapid growth, and resistance to conventional chemotherapy and radiation treatments, resulting in a median survival of about 14 months. To improve patient outcomes, novel therapeutic approaches are needed.

Cultural foundations of global health: a critical examination of universal child feeding recommendations.

There has been a rising call to decolonize global health so that it more fully includes the concerns, knowledge, and research from people all over the world. This endeavor can only succeed, we argue, if we also recognize that much of established global health doctrine is rooted in Euro-American beliefs, values, and practice rather than being culturally neutral. This paper examines the cultural biases of child feeding recommendations as a case in point.

Outcomes in stage IIA versus stage IIB/III in the PALLAS trial [ABCSG-42/AFT-05/PrE0109/BIG-14-13]).

Background: The PALLAS trial investigated the addition of palbociclib to standard adjuvant endocrine therapy to reduce breast cancer recurrence. This pre-specified analysis was conducted to determine whether adjuvant palbociclib benefited patients diagnosed with lower risk stage IIA disease compared to those with higher stage disease.

Methods: PALLAS was an international, multicenter, randomized, open-label, phase III trial, representing a public-private partnership between Pfizer, the Austrian Breast Cancer Study Group, and the U.

Functional heterogeneity of mesenchymal stem cells and their therapeutic potential in the K18-hACE2 mouse model of SARS-CoV-2 infection.

Background: Despite many years of investigation into mesenchymal stem cells (MSCs) and their potential for treating inflammatory conditions such as COVID-19, clinical outcomes remain variable due to factors like donor variability, different tissue sources, and diversity within MSC populations. Variations in MSCs' secretory and proliferation profiles, and their proteomic and transcriptional characteristics significantly influence their therapeutic potency, highlighting the need for enhanced characterization methods to better predict their efficacy. This study aimed to evaluate the biological characteristics of MSCs from different tissue origins, selecting the most promising line for further validation in a K18-hACE2 mouse model of SARS-CoV-2 infection.

Pretreatment with serine protease inhibitors impairs Leishmania amazonensis survival on macrophages.

Background: Leishmaniases are neglected tropical diseases with great clinical and epidemiological importance. The current chemotherapy available for the treatment of leishmaniasis presents several problems, such as adverse effects, toxicity, long treatment time, and parasite resistance. The discovery of new therapeutic alternatives is extremely essential, and the discovery of cellular targets is a tool that helps in the development of new drugs.

An interactive course program on nutrition for medical students: interdisciplinary development and mixed-methods evaluation.

Nutrition plays a crucial role in human and planetary health, as prevailing nutritional patterns significantly contribute to the global non-communicable disease pandemic. Moreover, the global food system is inextricably linked to planetary health deterioration. The relevance of nutrition for individual and planetary health is insufficiently addressed in German medical schools.

Chronic inflammation in post-acute sequelae of COVID-19 modulates gut microbiome: a review of literature on COVID-19 sequelae and gut dysbiosis.

Background: Long COVID or Post-acute sequelae of COVID-19 is an emerging syndrome, recognized in COVID-19 patients who suffer from mild to severe illness and do not recover completely. Most studies define Long COVID, through symptoms like fatigue, brain fog, joint pain, and headache prevailing four or more weeks post-initial infection. Global variations in Long COVID presentation and symptoms make it challenging to standardize features of Long COVID.

Chemical composition, and activity of the methanolic extract derived from against clinically relevant bacteria.

This study aimed to identify chemical compounds derived from Vassobia breviflora methanolic extract using ESI-ToF-MS and their antioxidant potential activity utilizing the following methods: total phenols, DPPH, and ABTS. The MTT assay measured cytotoxic activity, while DCFH-DA and nitric oxide assays were employed to determine reactive oxygen species (ROS) and reactive nitrogen species (RNS) levels using African green monkey kidney (VERO) and human keratinocyte (HaCat) cell lines. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were assessed in seven clinical isolates and nine ATCC strains.

Postoperative pain in primary root canal treatments after Er: YAG laser-activated irrigation: a systematic review and meta-analysis.

This systematic review aimed to compare postoperative pain in endodontic treatments using PIPS Er: YAG laser-activated irrigation (LAI) versus conventional needle irrigation. An electronic search was conducted to identify randomized clinical trials (RCT) investigating postoperative pain in patients who underwent root canal treatments in permanent teeth using PIPS Er: YAG laser-activated irrigation or conventional needle irrigation. Two reviewers performed study selection, data extraction, risk of bias assessment (RoB 2.

Effects of photobiomodulation in mitochondrial quantity, biogenesis and mitophagy-associated genes in breast cancer cells.

In this article, we aim to evaluate the effects of photobiomodulation on mitochondria quantity, biogenesis, and mitophagy-associated genes in breast cancer (BC) cells. Both models were irradiated with a low-power infrared laser (880 nm, 150 mW) and amber LED (617 nm, 1500 mW), alone or simultaneously. We evaluated the mRNA expression of PINK1 and PGC-1α genes, and the mitochondrial number was assessed based on the ratio of mitochondrial DNA/genomic DNA (mtDNA/gDNA).

Diversity of hemipterans in Eucalyptus spp. plantations in southeastern pará, Eastern Amazon, Brazil.

Brazil has 10.2 million hectares of reforestation, which account for 81% of the timber produced in the country. The order Hemiptera contains the main phytophagous species.

Potential of Lambda-Cyhalothrin for Controlling the Black Armyworm Spodoptera cosmioides (Walker) (Lepidoptera: Noctuidae): Toxicity and Midgut Histopathological Effects.

Caterpillars of the genus Spodoptera are the main pests in soybean and cotton crops and Spodoptera cosmioides causes more severe losses than other caterpillars in these agricultural crops. However, there are few recommended insecticides for controlling this pest. Lambda-cyhalothrin is a pyrethroid used to control a wide spectrum of arthropods including lepidopterans.

Sexual Mosaicism and Teratogenesis in Simulium guianense s.l. and S. incrustatum s.l. (Diptera: Simuliidae), Onchocerciasis vectors, from Environmentally Impacted Areas in the Yanomami Indigenous Land, Brazil.

Sexual mosaics in Simuliidae members have been detected at low frequencies in various locations worldwide. These phenotypic expressions are expected in very small amounts in any natural insect population and may result from inherent individual formation factors, such as hormonal or external elements, i.e.

Antagonist Action of Endophytic Metarhizium anisopliae Isolates on Development, Reproduction, and Host Selection of Grass Bug in Bermudagrass.

To understand the interactions of entomopathogenic fungi with forage plants and their influence on associated herbivorous, we evaluated the influence of endophytic colonization with three isolates (CEPAF_ENT 25, CEPAF_ENT 27, and IBCB 425) of Metarhizium anisopliae on Cynodon dactylon, regarding the biological and behavioral aspects of Collaria scenica, an emerging sucking pest in pastoral systems in Brazil. The application of suspensions at the base of plant (drench) was effective in promoting endophytic colonization, especially in the roots, with emphasis on isolates CEPAF_ENT25 and CEPAF_ENT27. Despite the significant reduction in damage caused by C.

Impact of monensin, virginiamycin, trace minerals, and yeast combination on animal performance, carcass and meat characteristics of finishing feedlot bulls fed high grain diet.

This study was realized to evaluate the influence of monensin, virginiamycin,trace minerals and yeast combination on animal performance, feed efficiency, in situ digestibility, feeding behavior, and carcass and meat characteristics from bulls finished in feedlot fed high-grain diet. A total of 36 (European vs. Nellore) bulls at 24 ± 3.

Tracing the relationship between the upper plate earthquake cycle and megathrust slip, the Atacama fault system in Northern Chile.

Inland-normal faulting is recognised as an important process following large subduction earthquakes. The lack of data limits the understanding of how normal fault reactivation relates to the subduction earthquake cycle. We characterised the palaeoseismology of the Atacama fault system (AFS) in the Chilean subduction zone.

Analysis of choroidal markers of activity in pachychoroid disease spectrum.

Purpose: To detect choroidal markers of disease activity in eyes with chronic active unilateral pachychoroid disease spectrum (PDS) compared to the non-active contralateral eyes, based on multimodal imaging and particularly indocyanine green angiography (ICGA).

Methods: An observational retrospective case-control study. The study evaluated individuals diagnosed with chronic active unilateral PDS (group 1) by comparing the eyes with the non-active fellow eyes (group 2).

A living library concept to capture the dynamics and reactivity of mixed-metal clusters for catalysis.

The exploration of ligated metal clusters' chemical space is challenging, partly owing to an insufficiently targeted access to reactive clusters. Now, dynamic mixtures of clusters, defined as living libraries, are obtained through organometallic precursor chemistry. The libraries are populated with interrelated clusters, including transient and highly reactive ones, as well as more accessible but less reactive species.

A Combined Treatment Proposal for the Empty Deep Pyriform Space.

Background: Deepening of the nasolabial fold (NLF), drooping of the nasal tip, and facial expressions perceived as angry face, are common esthetic concerns. However, no studies have correlated this set of signs and symptoms with common anatomical causes. We review anatomical considerations of the region and propose a combined treatment modality.

Metal stents versus plastic stents for drainage of pancreatic fluid collections: a systematic review and meta-analysis of randomized trials with trial sequential analysis.

Background And Aims: Pancreatic fluid collections (PFCs), including walled-off necrosis (WON), are significant complications of acute pancreatitis, and their management often involves drainage, although the optimal type of stent for this purpose remains uncertain. This meta-analysis aimed to compare metal versus plastic stents for endoscopic ultrasound (EUS)-guided drainage of PFCs.

Methods: We searched PubMed, Embase, and Cochrane Library for randomized controlled trials (RCTs) comparing metal with plastic stents for drainage of PFCs.

Sex-specific association between maternal childhood adversities and offspring's weight gain in a Brazilian cohort.

Maternal adverse childhood experiences (ACEs) are linked to negative health and developmental outcomes in offspring. However, whether maternal ACEs influence infant weight gain in the first months of life, and if this effect differs by infant sex, remains unclear. This study included 352 full-term newborns from low-risk pregnancies and their mothers in low-income settings in Brazil.

Identification of candidate genes involved in Zika virus-induced reversible paralysis of mice.

Zika virus (ZIKV) causes a variety of peripheral and central nervous system complications leading to neurological symptoms such as limb weakness. We used a mouse model to identify candidate genes potentially involved in causation or recovery from ZIKV-induced acute flaccid paralysis. Using Zikv and Chat chromogenic and fluorescence in situ RNA hybridization, electron microscopy, immunohistochemistry, and ZIKV RT-qPCR, we determined that some paralyzed mice had infected motor neurons, but motor neurons are not reduced in number and the infection was not present in all paralyzed mice; hence infection of motor neurons were not strongly correlated with paralysis.

Gingival margin interfacial microleakage, staining, and push-out strengths of gingival colored composite resins in denture bases.

Statement Of Problem: Staining at the gingival margin could impact denture longevity, but the behavior of gingival colored composite resins (GCCs) in this area remains unclear.

Purpose: This in vitro study evaluated surface staining, microleakage, and push-out bond strength at the gingival margin of artificial teeth, comparing two consistencies of GCCs with two resin base materials.

Material And Methods: Specimens included artificial teeth (Ivostar; Ivoclar AG) and two acrylic resin base materials: conventional (Ondacryl; Clássico) and high-impact (Diamond D; Keystone Industries) (n=300).