The Novel HLA-C*03:678 Allele Identified Using Nanopore Sequencing.

The novel HLA-C*03:678 allele differs from HLA-C*03:04:01:02 by single non-synonymous nucleotide substitution.

Quantum Chemical NMR Spectroscopic Structural Analysis in Solution: The Investigation of 3-Indoleacetic Acid Dimer Formation in Chloroform and DMSO Solution.

We present a DFT-PCM NMR study of 3-indoleacetic acid (3-IAA), used as a working example, including explicit solvent molecules, named PCM-nCHCl, PCM-nDMSO (n = 0, 2, 4, 8, 14, 20, and 25), to investigate the dimer formation in solution. Apart from well-known cyclic (I) and open (II) acetic acid (AA) dimers, two new structures were located on DFT-PCM potential energy surface (PES) for 3-IAA named quasicyclic A (III) and quasicyclic B (IV), the last one having N-H…O hydrogen bond (instead of O-H…O). In addition, four other structures having π-π type interactions named V, VI, VII, and VIII were also obtained completing the sample on the PES.

Deployment and transcriptional evaluation of nitisinone, an FDA-approved drug, to control bed bugs.

Background: Bed bugs are blood-feeders that rapidly proliferate into large indoor infestations. Their bites can cause allergies, secondary infections and psychological stress, among other problems. Although several tactics for their management have been used, bed bugs continue to spread worldwide wherever humans reside.

Health-related quality of life in Chagas cardiomyopathy: Development of a theoretical model.

Objective: Chagas disease can cause several complications, such as Chagas cardiomyopathy, the most severe clinical form of the disease. Chagas cardiomyopathy is complex and involves biological and psychosocial factors that can compromise health-related quality of life. However, it is necessary to establish interactions that significantly impact the health-related quality of life of this population.

Oral manifestations of dengue virus infection: a scoping review for clinical dental practice.

Background: Dengue virus (DENV) infection, a mosquito-borne disease, presents a significant public health challenge globally, with diverse clinical manifestations. Although oral dengue manifestations are uncommon, they can serve as crucial diagnostic indicators and impact patient management in dental practice. This scoping review aims to map the evidence on the oral manifestations associated with DENV infection and their clinical implications for dental practice.

Photobiomodulation and aquatic training reduce TNF-α expression and enhance muscle fiber area in Wistar rats with compensatory hypertrophy.

This study aims to assess the effects of aquatic training (AT) and its combination with photobiomodulation (PBM) on cytokine synthesis and plantar muscle morphology during compensatory hypertrophy (H) in Wistar rats. H was induced by bilateral ablation of synergistic muscles, and PBM using a laser (780 nm). AT involved 60 min sessions, 5 times/week, for 7 and 14 days.

Fungal lignocellulolytic enzymes: an in silico and full factorial design approach.

Efficient degradation of lignocellulosic biomass is key for the production of value-added products, contributing to sustainable and renewable solutions. This study employs a two-step approach to evaluate lignocellulolytic enzymes of Ceratocystis paradoxa, Colletotrichum falcatum, and Sporisorium scitamineum. First, an in silico genomic analysis was conducted to predict the potential enzyme groups produced by these fungi.

Hematopoietic stem cell transplantation in inborn errors of metabolism-a retrospective analysis on behalf of the pediatric disease working party from the Brazilian Society of Bone Marrow Transplantation and Cellular Therapy.

Hematopoietic stem cell transplantation (HSCT) is an established treatment for selected patients with inborn errors of metabolism. In this first report from the PDWP-SBTMO, we included 105 patients transplanted between 1988 and 2021 across six Brazilian HSCT centers. The most prevalent diseases were X-linked adrenoleukodystrophy (n = 61) and mucopolysaccharidosis (type I n = 20; type II n = 10), with a median age at HSCT of 8.

Label-free proteomic analysis of Duchenne and Becker muscular dystrophy showed decreased sarcomere proteins and increased ubiquitination-related proteins.

Muscular dystrophies (MD) are a group of hereditary diseases marked by progressive muscle loss, leading to weakness and degeneration of skeletal muscles. These conditions often result from structural defects in the Dystrophin-Glycoprotein Complex (DGC), as seen in Duchenne Muscular Dystrophy (DMD) and Becker Muscular Dystrophy (BMD). Since MDs currently have no cure, research has focused on identifying potential therapeutic targets to improve patients' quality of life.

Machine learning approach for noninvasive intracranial pressure estimation using pulsatile cranial expansion waveforms.

Noninvasive methods for intracranial pressure (ICP) monitoring have emerged, but none has successfully replaced invasive techniques. This observational study developed and tested a machine learning (ML) model to estimate ICP using waveforms from a cranial extensometer device (brain4care [B4C] System). The model explored multiple waveform parameters to optimize mean ICP estimation.

Global transcriptional modulation and nutritional status of soybean plants following foliar application of zinc borate as a suspension concentrate fertilizer.

The management of micronutrients, such as boron (B) and zinc (Zn), is critical for plant growth and crop yields. One method of rapid intervention crop management to mitigate nutritional deficiency is the foliar supply of B and Zn. Our study investigates the effect of foliar-supplied B and Zn availability on the global transcriptional modulation in soybean (Glycine max).

Content validation of drug problem-oriented clinical record.

Background: Worldwide, hospitals are responsible for restoring health. However, poorly executed processes in these institutions can lead to risks and harm to patients, especially in identifying drug related problems. This fact justifies the proposal of tools to support the diagnosis, management and resolution of these problems.

Human sleep and immunity: The role of circadian patterns.

It is well established that sleep promotes health and welfare. Literature data suggests that sleep is a recurrent resting state that performs multiple biological functions, such as memory consolidation and regulation of glucose, lipid metabolism, energy metabolism, eating behavior, and blood pressure, besides, regulating the immune system. These immunological functions depend on regular sleep and circadian rhythms, as both impact the magnitude of immune responses.

Shift work sleep disorder.

Shift work sleep disorder (SWSD) is a circadian rhythm sleep-wake disorders affecting individuals who work in nonstandard hours, particularly night shifts. It manifests as difficulty sleeping during the day and staying awake during work hours, leading to health issues. SWSD is not universally experienced by all shift workers, with about 30% affected.

Jacob Fidelis Ackermann (1765-1815): pioneer in the anatomical description of basilar invagination.

Jacob Fidelis Ackermann was a German Medical Doctor born in 1765 in the city of Rüdesheim. Between 1789 and 1815 years he was professor of medicine at the universities of Mainz and Heidelberg, teaching the disciplines of anatomy, physiology, botany, and natural history. In his famous work on basilar invagination, Ackermann described and illustrated the cranial base flattening in two skulls from Italy.

Relationship among depression, fatigue, and sleep after traumatic brain injury: The role of physical exercise as a non-pharmacological therapy.

Traumatic brain injury (TBI) is a burdensome condition frequently associated with an increased risk of psychiatric disorders. Although the exact molecular signaling pathways have not yet been fully defined, the compromised integrity of functional brain networks in regions such as the prefrontal cortex and anterior cingulate cortex has been linked to persistent symptoms, including depression, fatigue, and sleep disorders. Understanding how TBI affects neural physiology enables the development of effective interventions.

Behavior and oxidative stress evaluation of scorpion Tityus serrulatus (Lutz & Mello,1922) envenomation with genomic modulation and dopaminergic neutralization by antiscorpionic serum treatment.

Tityus serrulatus accident promote vast symptomatology related to toxins of the venom, which leads to a massive release of neurotransmitters, notably dopamine, affecting behavior and neurochemistry. The recommended treatment for envenomation is the antiscorpionic serum (SAEsc) administration. Related to this complexity of the Tityus serrulatus envenomation, this study aimed to assess organism responses to the venom, its impact on behavior, oxidative stress, neurochemistry, and genetic impacts, as well as the efficacy of SAEsc, especially concerning dopamine levels and genetic interactions.

Antimicrobial susceptibility of gram-negative bacteria: analysis from patients in a laboratory network in Brazil.

Objectives: This study aimed to describe the epidemiology and antimicrobial susceptibility patterns of gram-negative pathogens in Brazil from 2018 to 2020, addressing the gap in national data on healthcare-associated infections, using information from a private laboratory network.

Methods: A cross-sectional study was conducted using a database from Fleury hospital network, a private laboratory in Brazil. The analysis included blood, urine, and lower respiratory tract samples collected from January 2018 to June 2020.

Consensus recommendations for measuring the impact of contraception on the menstrual cycle in contraceptive clinical trials.

Objective: We sought to develop consensus recommendations for measurement and analysis of data on contraceptive-induced menstrual changes (CIMCs) in contraceptive clinical trials. We built upon previous standardization efforts over the last 50 years and prioritized input from a variety of global experts and current regulatory authority guidance on patient-reported outcomes.

Study Design: We completed a formal consensus-building process with an interdisciplinary group of 57 experts from 30 organizations and 14 countries in five global regions who work across academia, nonprofit research organizations, the pharmaceutical industry, and funding agencies.

Aerial urination suggests undescribed sensory modality and social function in river dolphins.

Scent marking through urine spraying is known to aid mate selection, territory marking and chemical communication in terrestrial, but not in aquatic mammals. We quantify an unusual aerial urination behaviour in botos (Inia geoffrensis) and discuss its potential functions. Between 2014 and 2018, we conducted land-based behavioural surveys on wild botos in central Brazil, recording the sequence, duration and social context of aerial urination.

Efficacy and safety of peroxide-based mouthrinse on whitening treatment: a randomized controlled clinical trial.

Objectives: This randomized controlled clinical trial evaluated the efficacy and safety of peroxide-based mouthrinse on whitening treatment. The patient's satisfaction and treatment longevity were also assessed.

Material And Methods: Participants (N=45) were randomly allocated to three different groups: WM (Whitening mouthrinse - 2.

Describing the landscape of nutrition- and diet-related randomized controlled trials: meta-research study of protocols published between 2012 and 2022.

Background: Publishing protocols promotes transparency and reproducibility. The scope and methods of protocols for nutrition- and diet-related randomized controlled trials (RCTs) have not been investigated yet.

Objective: Map the landscape of nutrition- and diet-related interventions research.

Lung cancer screening program quality indicators - review & recommendations - An International Association for the Study of Lung Cancer Delphi process study.

Introduction: Lung cancer screening (LCS) using low-dose-computed tomography reduces lung cancer mortality in high-risk individuals. Evaluating and monitoring LCS programs are important to ensure and improve quality, efficiency and participant outcomes. There is no agreement on LCS quality indicators (QIs).