3 results match your criteria: "Branch of M.M.Shemyakin Institute of Bioorganic Chemistry[Affiliation]"
Preparative in vitro mRNA synthesis using SP6 and T7 RNA polymerases.
Anal Biochem
June 1991
Branch of M. M. Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Pushchino, Moscow Region.
A method for preparative in vitro mRNA synthesis is presented. The method makes it possible to generate several hundred RNA copies per molecule of linearized DNA template. The protocol is applicable to the synthesis of RNAs of differing lengths (from 200 to 3000 bases), and both SP6 and T7 RNA polymerases can be used.
View Article and Find Full Text PDFFunctional expression in vitro of bovine visual rhodopsin.
Protein Eng
April 1990
Branch of M.M.Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences, Pushchino, Moscow Region, USSR.
Expression of functionally active bovine visual rhodopsin was achieved by sequential transcription and translation in vitro of rhodopsin gene cDNA with co-translational insertion of the protein into phosphatidylcholine liposomes. The recombinant rhodopsin has functional, spectral and immunochemical properties similar to those of natural rhodopsin from bovine retina. Two mutant rhodopsins, Cys316----Ser and Asp330----Asn, Asp331----Asn, were produced by oligonucleotide-directed mutagenesis.
View Article and Find Full Text PDFIn vitro synthesis of visual rhodopsin for a protein engineering study.
J Protein Chem
June 1989
Branch of M. M. Shemyakin Institute of Bioorganic Chemistry, Academy of Sciences of the USSR, Pushchino, Moscow region.