Poly(A)-binding protein promotes VPg-dependent translation of potyvirus through enhanced binding of phosphorylated eIFiso4F and eIFiso4F∙eIF4B.

The phosphorylation of eukaryotic translational initiation factors has been shown to play a significant role in controlling the synthesis of protein. Viral infection, environmental stress, and growth circumstances cause phosphorylation or dephosphorylation of plant initiation factors. Our findings indicate that casein kinase 2 can phosphorylate recombinant wheat eIFiso4E and eIFiso4G generated from E.

"If the college adjusted the prices…": comparing food secure and insecure college students during the COVID-19 pandemic.

We examined how students' food insecurity related to their demographic information, academic experiences, use of food programs, and reflections on food during the COVID-19 pandemic. 246 NYC undergraduates during the first 9 months of the pandemic. Students reported on food insecurity (eg, USDA's 10-item AFSSM), household income, impact of food insecurity on academics, GPA, and use of food programs.

Antimicrobial and Antioxidant Properties of , , , and - Traditional Plants Used in Central America.

The search for alternative naturally occurring antimicrobial agents will always continue, especially when emerging diseases like COVID-19 provide an urgency to identify and develop safe and effective ways to prevent or treat these infections. The purpose of this study was to evaluate the potential antimicrobial activity as well as antioxidant properties of commercial samples from four traditional medicinal plants used in Central America: , , , and . Ethanolic extracts were prepared from commercial products derived from the seeds or flowers of these plants.

SIRT7 and p53 interaction in embryonic development and tumorigenesis.

p53 is a hallmark tumor suppressor due in part to its role in cell cycle progression, DNA damage repair, and cellular apoptosis; its protein activity interrelates with the Sirtuin family of proteins, major regulators of the cellular response to metabolic, oxidative, and genotoxic stress. In the recent years, mammalian Sirtuin 7 (SIRT7) has emerged as a pivotal regulator of p53, fine-tuning its activity in a context dependent manner. SIRT7 is frequently overexpressed in human cancer, yet its precise role in tumorigenesis and whether it involves p53 regulation is insufficiently understood.

Drug Reprofiling to Identify Potential HIV-1 Protease Inhibitors.

The use of protease inhibitors in human immunodeficiency virus type 1 (HIV-1) treatment is limited by adverse effects, including metabolic complications. To address these challenges, efforts are underway in the pursuit of more potent and less toxic HIV-1 protease inhibitors. Repurposing existing drugs offers a promising avenue to expedite the drug discovery process, saving both time and costs compared to conventional de novo drug development.

Women Want Choices: Opinions from the Share.Learn.Shape Global Internet Survey About Multipurpose Prevention Technology (MPT) Products in Development.

Women need multipurpose prevention technologies (MPTs) to simultaneously prevent sexually transmitted infections (STIs), including HIV, with or without contraception. User feedback early in product development is critical for maximizing uptake and continuation. Our global online survey (April 2017-December 2018) explored women's opinions about MPT formulations in development (e.

The synchronous group virtual simulation experience: Associate degree nursing students' perceptions.

The use of virtual simulations exponentially increased as nursing schools experienced an urgent need to integrate online educational technologies during the COVID-19 pandemic. This qualitative descriptive study was conducted to explore associate degree nursing students' perceptions about the comprehensive Synchronous Group Virtual Simulation educational strategy. This strategy was developed based on the National League for Nursing Jeffries Simulation Theory (JST) and the Healthcare Simulation Standards of Best Practice, including structured synchronous prebriefing and debriefing stages.

Essential oils block cellular entry of SARS-CoV-2 delta variant.

Aiming to fill a gap in the literature, we aimed to identify the most promising EOs blocking in vitro cellular entry of SARS-CoV-2 delta variant without conferring human cytotoxicity and provide insights into the influence of their composition on these activities. Twelve EOs were characterized by gas chromatography coupled to mass spectrometry. The antiviral and cytotoxicity activities were determined using the cell-based pseudoviral entry with SARS-CoV-2 delta pseudovirus and the XTT assay in HeLa cells expressing human angiotensin-converting enzyme 2 (HeLa ACE-2), respectively.

Small Molecules Targeting SARS-CoV-2 Spike Glycoprotein Receptor-Binding Domain.

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused the coronavirus disease 2019 (COVID-19) pandemic. Several variants of SARS-CoV-2 have emerged worldwide. These variants show different transmissibility infectivity due to mutations in the viral spike (S) glycoprotein that interacts with the human angiotensin-converting enzyme 2 (hACE2) receptor and facilitates viral entry into target cells.

Sample Preparation for Rapid Lipid Analysis in Drosophila Brain using Matrix-assisted Laser Desorption/Ionization Mass Spectrometry Imaging.

Lipid profiling, or lipidomics, is a well-established technique used to study the entire lipid content of a cell or tissue. Information acquired from lipidomics is valuable in studying the pathways involved in development, disease, and cellular metabolism. Many tools and instrumentations have aided lipidomics projects, most notably various combinations of mass spectrometry and liquid chromatography techniques.

Laparoscopic Gastric Band Placement in Combination With Sleeve Gastrectomy for Advanced Weight Loss: A Case Report.

Laparoscopic adjustable gastric banding (LAGB) over laparoscopic sleeve gastrectomy (LSG) is a rare bariatric surgery. The objective of this case report is to add to the limited literature regarding this procedure. LAGB plus LSG is an effective bariatric procedure for patients that desire additional weight loss who do not qualify for or want gastric bypass.

Post-secondary online learning in the U.S.: an integrative review of the literature on undergraduate student characteristics.

Even prior to the COVID-19 pandemic, online learning had become a fundamental part of post-secondary education. At the same time, empirical evidence from the last decade documents higher dropout online in comparison to face-to-face courses for some students. Thus, while online learning may provide students access to post-secondary education, concerns about academic momentum and degree attainment dominate the higher education online learning landscape.

Biology, chemistry, and pharmacological activity of (Bignoniaceae) and (Clusiaceae) - a review.

and are two West African medicinal plants traditionally used to treat or alleviate various medical conditions such as skin ailments, respiratory disorders, and digestive problems. Phytochemical analyses indicated the presence of bioactive constituents, including flavonoids and phenolic acids, suggesting that the extracts of these two plants can interfere with reactive oxygen species-induced oxidative stress, inflammation, and microbial growth. This paper reviews the biochemical properties and the antioxidant, anti-inflammatory, and antibacterial activities of these two relevant West African medicinal plants.

Developing end-point methods for absolute binding free energy calculation using the Boltzmann-quasiharmonic model.

Understanding the physical forces underlying receptor-ligand binding requires robust methods for analyzing the binding thermodynamics. In end-point binding free energy methods the binding free energy is naturally decomposable into physically intuitive contributions such as the solvation free energy and configurational entropy that can provide insights. Here we present a new end-point method called EE-BQH (Effective Energy-Boltzmann-Quasiharmonic) which combines the Boltzmann-Quasiharmonic model for configurational entropy with different solvation free energy methods, such as the continuum solvent PBSA model and the integral equation-based 3D-RISM, to estimate the absolute binding free energy.

Results of a phase 1, randomized, placebo-controlled first-in-human trial of griffithsin formulated in a carrageenan vaginal gel.

HIV pre-exposure prophylaxis (PrEP) is dominated by clinical therapeutic antiretroviral (ARV) drugs. Griffithsin (GRFT) is a non-ARV lectin with potent anti-HIV activity. GRFT's preclinical safety, lack of systemic absorption after vaginal administration in animal studies, and lack of cross-resistance with existing ARV drugs prompted its development for topical HIV PrEP.

Conjunctional Concepts: The Conceptual Teaching Technique in Biology Classes.

Conceptual teaching relies on concepts in conjunction with a bigger conceptual category (or categories). Revealing and using conceptual conjunctions in the science classroom means teaching conceptually. To make conceptual teaching more accessible to biology instructors, a three-concept model was described to be used during a lecture.

Antiviral activity of aframomum melegueta against severe acute respiratory syndrome coronaviruses type 1 and 2.

Plant-based compounds with antiviral properties against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been identified in through computational models. The seed extract have been traditionally used to treat different illnesses. In this study, ethanolic extracts were prepared for six commercial samples of seeds.

Algal and Cyanobacterial Lectins and Their Antimicrobial Properties.

Lectins are proteins with a remarkably high affinity and specificity for carbohydrates. Many organisms naturally produce them, including animals, plants, fungi, protists, bacteria, archaea, and viruses. The present report focuses on lectins produced by marine or freshwater organisms, in particular algae and cyanobacteria.

If you want to develop an effective autism training, ask autistic students to help you.

Autistic university students are often left out because people do not understand autism. We wanted to help people understand autism. Most autism trainings are not made by autistic people.

Griffithsin and Carrageenan Combination Results in Antiviral Synergy against SARS-CoV-1 and 2 in a Pseudoviral Model.

Over 182 million confirmed cases of COVID-19 and more than 4 million deaths have been reported to date around the world. It is essential to identify broad-spectrum antiviral agents that may prevent or treat infections by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) but also by other coronaviruses that may jump the species barrier in the future. We evaluated the antiviral selectivity of griffithsin and sulfated and non-sulfated polysaccharides against SARS-CoV-1 and SARS-CoV-2 using a cytotoxicity assay and a cell-based pseudoviral model.

When Did I Become a Victim? Exploring Narratives of Male Childhood Sexual Abuse.

This report presents narrative data of adult men self-identifying as victims of sexual abuse occurring in childhood or adolescence (CSA). Preliminary data were derived from written public stories posted by men to the Internet ( = 90). Many of these accounts indicate same-sex sexual abuse (male victim, male perpetrator).

Saving private W. H.: The surgical experiences of Dugald Blair Brown (1847-1896): Lieutenant-Colonel, FRCS, Edin., AMD.

Dugald Blair Brown, a military surgeon and Fellow of the Royal College of Surgeons, Edinburgh, published twelve papers containing 77 case studies of gunshot wounds that he had treated in the Anglo-Zulu War of 1879 and in the First Anglo-Boer War of 1880-1881. Brown devised a "conservative" method of surgery, the early development of which had been influenced by Thomas Longmore (1816-1895), Joseph Lister (1827-1912), F. J.