Generating long-read sequences using Oxford Nanopore Technology from Diospyros celebica genomic DNA.

Objectives: Development of sequencing technology has opened up vast opportunities for tree genomic research in the tropics. One of the aforesaid technologies named ONT (Oxford Nanopore Technology) has attracted researchers in undertaking testings and experiments due to its affordability and accessibility. To the best of our knowledge, there has been no published reports on the use of ONT for genomic analysis of Indonesian tree species.

Effect of Low Linear Growth and Caregiving with Poor Psychosocial Aspects on Cognitive Development of Toddlers.

The purpose of this study was to analyze effect of linear growth and psychosocial aspects of caregiving on cognitive development of toddlers. Methods: Longitudinal studies were carried out from pregnancy to three years old children in Bogor. The collected data included body height, nutritional status based on height by age index, morbidity, aspects of caregiving, and cognitive development of three years old children.

Relation of Body Mass Index, Waist Circumference, and Body Fat Percentage to Lipid Profile and Oxidative Stress Markers in Menopausal Women.

The degenerative diseases in menopause-age women has been continued to increase every year. This study aimed to analyze the relation of body mass index, waist circumference, and body fat percentage on blood biochemical markers of lipid and oxidative stress in menopausal women. This randomized control trial (RCT) was conducted using a cross-over design.

Assessment of Anemia Status on Physical Development Skills of Children Under Two Years Old in Aceh, Indonesia.

Anemia and iron deficiency can cause detrimental effects on brain development, and continued iron deficiency anemia is known to interfere with development which results in a permanent delay in mental and Motor development. This study investigated the impact of anemia on physical development skills of under two years children in aceh besar district. A cross-sectional study design was applied to 102 under-two years children selected using cluster random sampling technique using village as cluster.

The Influence of Nutritionist-Based Food Service Delivery System on Food and Nutrient Quality of School Lunch Program in Primary Schools in Indonesia.

Unlabelled: It is well-established that school feeding program (SFP) has been provided significant nutritional and health beneficial effects to student namely. SFP or school meal services program without nutritionist have lack of food delivery system, nutrient quality and health promotion. Thus, the aim of this study was to analyze the influence of nutritionist-based food service delivery system on food and nutrient quality of school lunch program in primary school.

Impact of Multi-Component Nutrition Intervention on Teachers, Parents, and Students toward the Knowledge and Attitude about Fruit and Vegetable Consumption.

Fruit and vegetable (FV) consumption is one of the important components in balanced nutrition, but FV consumption in elementary school students (ESS) grade 5 and 6 was still low. The purpose of this study was to determine the impact of multicomponent nutrition intervention in teachers, parents, and students toward the knowledge and attitude about FV consumption. The study used a quasi-experimental design with a pre-post intervention method with nutrition education to three groups subject using comic and pocketbook in Nganjuk, East Java from February to May 2017.

Effect of Nutrition Education and Multi-Nutrient Biscuit Interventions on Nutritional and Iron Status: A Cluster Randomized Control Trial on Undernourished Children Aged 6-23 Months in Aceh, Indonesia.

Undernutrition and iron deficiencies on under-five children in Indonesia remain high and very closely related to inadequate complementary feeding. This study investigated the effect of weekly nutrition education by home visite using the food monitoring card (FMC) models and daily provision multi-nutrient biscuits and combination on growth and reduction of iron deficiency and anemia among underweight children aged 6-23 mo in Aceh Indonesia. A 6-mo, cluster randomized, control trial was conducted on 121 children received nutrition education (NE), multi-nutrient biscuit (MNB), combination both nutrition education and biscuits (NE+MNB), and control group.

Analysis of the Effect of Fish Bars Made of Bilih Fish (Mystacoleuseus padangensis Blkr) Flour to Reduce Oxidative Stress in a Diabetic Rat Model.

This study aims to analyze the effect of Bilih fish bars (BFB) on oxidative stress based on the levels of MDA and SOD in diabetic rat models. This study used a Randomized Complete Design (RCD). Forty white male rats of the Sprague-Dawley strain were placed into the following five groups: normal and diabetic rats that were fed either the standard feed and metformin, BFB, BFB, and metformin or BFF with a zinc dose of 0.

Calcium Bioavailability of Tempe and Boiled Soybean Flours and Its Effect on Osfemurs in Experimental Rats.

Tempe is a nutritious food that contains both macronutrients and micronutrients in an adequate amount. Tempe contains a high amount of calcium, so it is an affordable food product that can be purchased by most people in Indonesia. Calcium deficiency can lead to several diseases and disorders such as osteoporosis, hypertension, heart disease, and nerve damage.

Equivalence Test between the Physicochemical Properties of Transgenic and Non-Transgenic Soy Flour.

Soybeans are a source of plant-based protein with a fairly complete composition of essential amino acids. Most of the soybean raw material in Indonesia originates from the United States of America where around 75% of the soybeans are transgenic soybeans (Roundup Ready GMO). One of the easily produced and practical soybean products is soy flour.

Flavonoid Database Based on Indonesian Foods.

Background And Objectives: The prevalence of NCDs (Non-communicable Diseases) was increasing in Indonesia. The antioxidants have been known as a potential nutrient that prevent and reduce the risk of the NCDs. Flavonoid is the most abundant antioxidant in plant-source diet and commonly consumed in Indonesia.

Determinant Factors of Maternal Knowledge on Appropriate Complementary Feeding of Children Aged 6-23 Months in Aceh.

The aim of this study was determine mothers knowladge on appropriate complementary feeding of children aged 6-23 mo. A cross-sectional design was applied on 392 mothers of children aged 6-23 mo selected through a cluster sampling in Aceh Besar District Aceh Province Indonesia. Data on mothers knowledge and determinant factors included socio-demographic of mothers were collected using valid and reliable structured questionnaire.

The Physicochemical Properties of Tempe Protein Isolated from Germinated and Non-Germinated Soybeans.

Tempe is an indigenous Indonesian source of protein. In this study, the tempe was produced from Grobogan soybeans with two types of treatment, germinated and non- germinated. The two types of tempe produced were then processed into protein isolates, tempe protein isolate from non-germinated soybeans (NGTI) and tempe protein isolate from germinated soybeans (GTI).

The Effect of Nutrition Education on School-Aged Children's Consumption Pattern, Knowledge and Practice in Bringing Well-Balanced Menu for Lunch.

This study aimed to analyze the effect of nutrition education on the consumption pattern, knowledge, and practice in bringing a well-balanced menu (WBM) for lunch among school-aged children. The study was quasi-experimemtal research using one group with pre- and post-test. Subjects were 52 students in fifth grade at SDN Babakan Dramaga 04, Bogor.

Coverage and Adherence of Weekly Iron Folic Acid Supplementation among School Going Adolescent Girls in Indonesia.

Unlabelled: The Weekly Iron Folic Acid (WIFA) supplementation program for school going adolescent girls has been implemented by Indonesian Government since 2016. The objective of this study was to assess the coverage and adherence toward the WIFAS among school going adolescent girls, as part of a baseline assessment of the new intervention.

Methods: A cross-sectional survey was conducted in East Java (EJ) and East Nusa Tenggara (ENT) in year 2018.

Phenazine-1-carboxylic acid (PCA) produced by Paraburkholderia phenazinium CK-PC1 aids postgermination growth of Xyris complanata seedlings with germination induced by Penicillium rolfsii Y-1.

Symbiosis of Penicillium rolfsii Y-1 is essential for the seed germination of Hawaii yellow-eyed grass (Xyris complanata). However, the local soil where the plants grow naturally often suppresses the radicle growth of the seedlings. This radicle growth was drastically restored by coinoculation of Paraburkholderia phenazinium isolate CK-PC1, which is a rhizobacterium of X.

The Ethanol Extract of Marine Sponge Aaptos suberitoides Suppress Cell Viability, Cell Proliferation and Cell Migration in HER2-Positive Breast Cancer Cell Line.

Objective: This study aimed to investigate the cytotoxicity, anti-proliferation and anti-migration effect of the ethanol extract of Aaptos suberitoides on trastuzumab-resistant HER2+ breast cancer cell line.

Methods: Aaptos suberitoides was collected from Tinjil Island, Banten, Indonesia, and was processed with maceration and ethanol extraction. HCC-1954 cells were treated with the ethanol extract and then followed by 3- [4, 5-dimethylthiazol-2-yl] -2.

Assessment of biphasic calcium phosphate 70/30 alginate scaffold on the tibia in pigs.

Background And Aim: Calcium phosphate bioceramics have been used for at least a decade, and many investigations have focused on the use of hydroxyapatite (HA) derivative in the regeneration of bone defects. Biphasic calcium phosphate (BCP) is a biomaterial composed of HA and beta-tricalcium phosphate (BCP), with a structure similar to bone. The aim of the study was to determine the influence of the BCP/alginate scaffold on tissue growth, blood, the lungs, and the electrical activity of the heart during bone healing in the tibia of pig.

Spatial and temporal distribution of microplastic in surface water of tropical estuary: Case study in Benoa Bay, Bali, Indonesia.

In 2010, Indonesia estimated as the second-largest country in the world that donates plastic to the sea. This study aims to investigate the spatial and temporal distribution of microplastics in tropical estuaries. The sampling was carried out in Benoa Bay with four repetitions representing the wet and dry seasons.

Micro- and mesoplastics release from the Indonesian municipal solid waste landfill leachate to the aquatic environment: Case study in Galuga Landfill Area, Indonesia.

Small-sized plastic debris (micro- and mesoplastics) are emerging pollutants and widely detected in aquatic environments. However, micro- and mesoplastics pollution research with regard to landfills is limited. In this study, the occurrence, characteristics, and possible release of micro- and mesoplastic waste from the Galuga landfill leachate to the aquatic environment were studied.

Novel Allergen Discovery through Comprehensive De Novo Transcriptomic Analyses of Five Shrimp Species.

Shellfish allergy affects 2% of the world's population and persists for life in most patients. The diagnosis of shellfish allergy, in particular shrimp, is challenging due to the similarity of allergenic proteins from other invertebrates. Despite the clinical importance of immunological cross-reactivity among shellfish species and between allergenic invertebrates such as dust mites, the underlying molecular basis is not well understood.

Unprecedented plastic-made personal protective equipment (PPE) debris in river outlets into Jakarta Bay during COVID-19 pandemic.

Increased plastic uses during COVID-19 pandemic challenges efforts to reduce marine plastic debris. Despite recent observations of increased plastic-made personal protection equipment (PPE) waste in coastal areas, comparative data before and during the pandemic lacked. We present in situ monitoring data on riverine debris releases into Jakarta Bay, Indonesia, during COVID-19 pandemic relative to the 2016 baseline data.

In vitro reconstitution of dynamically interacting integral membrane subunits of energy-coupling factor transporters.

Energy-coupling factor (ECF) transporters mediate import of micronutrients in prokaryotes. They consist of an integral membrane S-component (that binds substrate) and ECF module (that powers transport by ATP hydrolysis). It has been proposed that different S-components compete for docking onto the same ECF module, but a minimal liposome-reconstituted system, required to substantiate this idea, is lacking.

Shotgun metagenomic analysis reveals new insights into bacterial community profiles in tempeh.

Objective: Amplicon sequencing targeting 16S ribosomal RNA (rRNA) has been widely used to profile the microbial community from fermented food samples. However, polymerase chain reaction (PCR) steps on amplicon sequencing analysis and intragenomic heterogeneity within 16S rRNA are believed to contribute to bias in estimating microbial community composition. As potential paraprobiotics sources, a comprehensive profiling study of tempeh microbial ecology could contribute to tempeh product development.