Optimized thermosensitive liposomes for selective doxorubicin delivery: formulation development, quality analysis and bioactivity proof.

In our study we examined thermosensitive liposomal formulations (TL) from the perspective to minimize the general toxicity drawbacks of chemotherapy. The TL become active in response to local hyperthermia (LH), and remain inactive at physiological conditions. Here, we formulated novel doxorubicin loaded thermoliposomes (Dox-TL) with optimized characteristics and tested their biological activity in vitro.

Olivomycin induces tumor cell apoptosis and suppresses p53-induced transcription.

Olivomycin (DNA-binding antibiotic) in nanomolar concentrations induces apoptosis of human tumor cells and inhibits p53-dependent transcription of the reporter gene (basal and induced by antitumor drugs). Olivomycin aglycon induces no cytotoxicity and does not block transcription.

[Search for biological remedies for fungi of the Candida genus].

51 bacterial strains were studied with a view to find out anticandidial activity; of these, 15 strains were found to have activity, more on less expressed with respect to fungi of the genus Candida. In most cases the anticandidial activity of an antagonist strain was manifested to a similar degree against several Candida strains. The preliminary analysis of antifungal substances produced by microbial strains makes it possible to suggest their antibiotic nature.