77 results match your criteria: "Biotechnology Center of Borj-Cedria[Affiliation]"

The current research explored for the first time the effect of Salvia officinalis L. (Sage) essential oil (EO) on Alloxan-induced diabetes in male Wistar rats. Sage EO was extracted by a Clevenger apparatus and analyzed by GC-FID and GC-MS.

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Enhanced B-phycoerythrin production by the red microalga Porphyridium marinum: A powerful agent in industrial applications.

Int J Biol Macromol

December 2018

Laboratoire de Biotechnologie Végétale Appliquée à l'Amélioration des Cultures, Faculty of Sciences of Sfax, University of Sfax, Sfax, Tunisia. Electronic address:

The production of B-phycoerythrin (B-PE) from the red microalga Porphyridium marinum was optimized before to purify it and subsequently study its antioxidant activities. NaNO KHPO and metal traces concentrations of the culture medium, and luminosity parameters were chosen, according to the Plackett-Burman design, as the most influent factors on the B-PE production by P. marinum.

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Analysis of physiological traits in the response of Chenopodiaceae, Amaranthaceae, and Brassicaceae plants to salinity stress.

Plant Physiol Biochem

November 2018

Plant Nutrition Department, CEBAS-CSIC, Campus de Espinardo, 30100, Murcia, Spain. Electronic address:

Soil salinity is one of the main factors affecting plant growth. Dissection of plant response to salinity into physiological traits may result a simple approximation than the overall response that may influence many aspects of the plant. In the present study two factors were considered to evaluate the correlation of different physiological variables in the plant response to salinity.

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This work focuses on the variability of (Forssk.) Webb bioactive compounds as a function of the plant cycle. The main results showed that it exhibited the highest percentage of polyunsaturated fatty acids, along with superior levels of vitamin C and total phenolic compounds (66.

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essential oil ameliorates pasteurization efficiency.

J Food Sci Technol

September 2018

Laboratory of Aromatic and Medicinal Plants, Biotechnology Center of Borj-Cédria, BP 901, 2050 Hammam-Lif, Tunisia.

This work aims to characterize essential oil and to investigate its preservative effects on raw milk quality alone or combined to pasteurization heat treatment. To study its preservative effect, 1 mg l of essential oil, characterized by GC-MS, was added to pasteurized or raw milk. The evaluation of milk quality was made by consulting samples total acidities, peroxide values and their total microbial counts, as compared to control milks.

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The increasing anthropologic pressure and the modernization of agriculture have led to a forsaking of pearl millet traditional cultivars, inducing a progressive loss of the genetic variability encompassed in this locally adapted germplasm. Imperatively, national efforts based on robust data gleaned from genetic surveys have to be undertaken in order to set up suitable conservation priorities. In this study, in addition to the assessment of the genetic diversity and population structure among and within a set of seven pearl millet landrace populations from coastal North Africa, demographic and phylogenetic data, conservation priority scores were calculated according to Vane-Wright et al.

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The use of synthetic food additive and the appearance of antibiotic resistance are at the basis of important human health problems. The substitution of synthetic compounds with new natural substances extracted from plants or microorganisms is therefore the ideal solution to this scourge. The objective of this work was to evaluate the phyto-constituents (polyphenols, flavonoids and condensed tannins), and to test the biological activities (antioxidant, antibacterial and antiviral) of the Ajuga iva (L) aerial part extracts.

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Background: Virgin olive oil is appreciated for its particular aroma and taste and is recognized worldwide for its nutritional value and health benefits. The olive oil contains a vast range of healthy compounds such as monounsaturated free fatty acids, especially, oleic acid. The SAD.

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The present work was developed to evaluate the in vitro antioxidant, antibacterial, antileishmanial and cytotoxic activities of Echium arenarium (Guss) extracts, and to analyze their phytochemical composition. The highest content of total phenolic compounds was obtained in the ethyl acetate extract which showed the best DPPH scavenging activity and β-carotene bleaching inhibition (IC = 1.1 and 9.

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Host resistance and fungicide treatments are cornerstones of plant-disease control. Here, we show that these treatments allow sex and modulate parenthood in the fungal wheat pathogen Zymoseptoria tritici. We demonstrate that the Z.

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Hybridity and the genuineness of hybrids are prominent characteristics for quality control of seeds and thereby for varietal improvement. In the current study, the cross between two local barley genotypes (Ardhaoui: female; Testour: male) previously identified as susceptible/tolerant to salt stress in Tunisia was achieved. The hybrid genetic purity of the generated F putative hybrids and the fingerprinting of the parents along with their offspring were assessed using a set of 17 nuclear SSR markers.

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Olive (Olea europaea L.) is an economically important crop for the Mediterranean basin, where prolonged drought and soil salinization may occur. This plant has developed a series of mechanisms to tolerate and grow under these adverse conditions.

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Grapevine is an important fruit crop cultivated worldwide. Previously, we have reported the characterization of a salt stress-inducible gene Vv-α-gal/SIP isolated from the tolerant grapevine cultivar Razegui. In this study, we performed functional studies in both Escherichia coli and tobacco systems to gain more insights in the role of the Vv-α-gal/SIP gene.

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Electrophoresis-Based Proteomics to Study Development and Germination of Date Palm Zygotic Embryos.

Methods Mol Biol

April 2018

Agroforestry and Plant Biochemistry and Proteomics Research Group, Department of Biochemistry and Molecular Biology, University of Cordoba-CeiA3, Cordoba, Spain.

Proteomics has become an important and powerful tool in plant biology research. To establish a proteomic reference map of date palm zygotic embryos (ZE), we separated and identified proteins from zygotic embryos during different developmental and germination phases using one, two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. Proteins are extracted with trichloroacetic acid (TCA)/acetone-phenol and resolved by gel electrophoresis.

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Drought is a major factor limiting crop production worldwide. The objective of this study was to test whether pre-exposure to drought can enhance the subsequent drought response of a drought-sensitive variety of olive cv. .

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Identification and characterization of single nucleotide polymorphism markers in FADS2 gene associated with olive oil fatty acids composition.

Lipids Health Dis

July 2017

Molecular and Cellular Screening Processes: Research group, Microorganisms and Biomolecules Laboratory, Centre of Biotechnology of Sfax, BP '1177' Sidi Mansour road, 3018, Sfax, Tunisia.

Background: Genotyping of the FAD2.1 and FAD2.3 polymorphisms in the fatty acid desaturase 2 gene (FADS2) shows that they are associated with the fatty acids composition of olive oil samples.

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The objective of this study is to evaluate the effect of either a solution of Thymus capitatus essential oil or its nanoemulsion on the quality of milk contaminated by bacteria. After 24h of S. aureus inoculation, bacterial growth reached 202×10(3)CFU/ml in the presence of the essential oil while it was limited to 132×10(3)CFU/ml when treated with nanoemulsion.

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In the current study, the phenolic composition, antioxidant and antimicrobial activities of extracts from Rubus ulmifolius Schott leaves harvested in four localities (Sejnen, Tabarka, Faija and Ain drahem) in Tunisia were investigated for the first time. Great differences were found for the chemical composition, total phenol contents and biological activities among the evaluated extracts. HPLC analysis of methanolic extracts showed that the dominant compounds were kaempferol 3-O-rutinoside and naringenine.

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Reaumuria vermiculata is a xero-halophytic specie widely distributed in the south of Tunisia. In the current study, antioxidant, anti-inflammatory and anticancer activities of Reaumuria vermiculata shoot extracts as well as its phenolic compounds were investigated in different solvent extracts (hexane, dichloromethane, methanol and water). Results showed a strong antioxidant activity, using the ORAC method and a cell based-assay, in methanol extract as well as an important phenolic composition (117.

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Giant fennel (Ferula communis L.) is well known in folk medicine for the treatment of various organ disorders. The biological importance of members of genus Ferula prompted us to investigate the leaves of the endangered Tunisian medicinal plant F.

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Article Synopsis
  • Researchers isolated 80 endophytic bacteria from healthy tomato plant tissues and identified four strains (BL1, BT5, BR8, BF11) that inhibit the growth of Botrytis cinerea, a fungus causing gray mold, with inhibition rates between 27% and 53%.
  • The selected bacterial strains were linked to Bacillus species, which are known for producing lipopeptides that effectively inhibit pathogen growth, supported by morphological, biochemical, and molecular analyses including 16S rDNA sequencing.
  • Further analysis revealed that these bacteria produce various antibiotics (like fengycin, surfactin, and iturin) and showed potential to reduce disease severity in plant tests, suggesting their application in biological control of plant diseases
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Background: Acute coronary syndromes (ACS) are complex and polygenic diseases which are a real problem of public health. These syndromes require multidisciplinary studies to understand the pathogenesis mechanisms and metabolic interactions between different risk factors.This study aimed to explore the variation of two coronary risk parameters not mentioned by Framingham cohorts, hyperhomocysteinemia and endothelin-1 (ET-1) in Tunisian coronary and the study of the variation of these parameters based on various cardiac risk factors and metabolic relationship between them.

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Zymoseptoria tritici is an economically important pathogen of wheat. However, the molecular basis of pathogenicity on wheat is still poorly understood. Here, we present a global survey of the proteins secreted by this fungus in the apoplast of resistant (cv.

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In 2011, common symptoms of grapevine dieback were frequently observed in 2- to 5-year-old table grape (Vitis vinifera L.) cvs. in four vineyards located in northern Tunisia.

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