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Biotechnology Center of Borj-Cedria[Aff... Publications | LitMetric

77 results match your criteria: "Biotechnology Center of Borj-Cedria[Affiliation]"

This study aimed to evaluate the impact of four biochar concentrations (0, 2, 5, and 8%) on single and interactive effects of salinity and drought stresses on the morphological, physiological, and photosynthetic parameters of faba bean plants. PCA analysis showed that plants displayed different behavior under non-stressed and stressed conditions. The most discriminating quantitative characters were related to plant biomass production and photosynthesis, especially shoot dry mass, root dry mass, plant fresh mass, internal CO concentration, net CO assimilation rate, and relative water content.

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  • Researchers developed zirconium-doped PbO anodes on titanium substrates using a spin coating method to effectively remove the antibiotic ampicillin from water.
  • Characterization techniques like SEM, AFM, XRD, and EIS revealed that adding 10% zirconium significantly improved the anode's morphology, surface roughness, and electrochemical performance compared to other configurations.
  • The 10% Zr-doped anode exhibited the highest efficiency in degrading ampicillin into ampicilloic acid, indicating it as the optimal composition for wastewater treatment involving antibiotics.
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How does NaCl enhance Cd tolerance in Inula crithmoides L? Insights into Cd uptake, compartmentalization, and chelation.

Chemosphere

October 2024

Higher Institute of Arts and Crafts of Tataouine, University of Gabes, Rue OmarrEbenkhattab 6029 Zerig-Gabes, Tunisia; Laboratory of pastoral ecosystems and promotion of spontaneous plants and associated microorganisms, Institute of arid land, 4100, Medenine, University of Gabes, Tunisia. Electronic address:

This study investigated the effect of NaCl on the uptake, translocation, compartmentalization, and chelation of cadmium (Cd) in the halophyte Inula crithmoides. Seedlings were subjected hydroponically for 21 days to 25 and 50 μM Cd applied alone or combined with 100 mM NaCl. Findings revealed that, Cd alone induced intense chlorosis and necrosis and altered plant development resulting in diminished biomass production.

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  Our study aimed to optimize carob antioxidant extraction for use in the food, cosmetic, and pharmaceutical industries. Maceration investigated ethanol concentration, extraction temperature, and time, while ultrasound-assisted extraction examined ethanol concentration, ultrasound power, and time. A central composite design with 19 experimental points assessed the influence of variables on total yield, total phenolic content, and antioxidant activity.

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  • Antibiotic resistance and biofilm formation in Gram-negative bacteria pose significant challenges for infection control, particularly through the Quorum Sensing (QS) mechanism.
  • Myrtle leaf extract demonstrated a potent anti-QS effect, inhibiting 80% of violacein production and significantly reducing various virulence factors like biofilm formation and protease activity at sub-MIC concentrations.
  • The study identified three phenolic compounds from the extract, with 3,5-di-galloylquinic acid showing the strongest interaction with the CviR QS receptor, indicating potential for developing new QS inhibitors against resistant pathogens.
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  • * Major compounds identified in the EO included α-pinene and -cadinol, but the oil showed limited antioxidant activity, failing to neutralize certain free radicals effectively.
  • * The EO demonstrated strong leishmanicidal effects against promastigote and amastigote forms, with the components showing better binding to the enzyme CYP51 compared to the conventional drug fluconazole, suggesting it could be a promising alternative treatment for leishmaniasis.
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This study endeavoured to capitalize on prickly pear by-products for the optimization of molasses formulation, targeting elevated antioxidant capacities and superior sugar contents. Through robust statistical modelling, the optimal cooking parameters-temperature (70-80 °C) and duration (60-90 min)-were determined, guided by responses of antioxidant activity and Brix value. A D-Optimal mixture design further delineated the ideal proportions of molasses components (pulp, peel, and seeds).

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Comparative chemical composition and antioxidant activity of olive leaves L. of Tunisian and Algerian varieties.

Heliyon

December 2023

Laboratory of Materials Molecules and Applications, Preparatory Institute for Scientific and Technical Studies, Carthage University, B.P:51, La Marsa, 2075 Tunis, Tunisia.

The main objective of this research was to understand the effect of different solvents (ethanol, ethyl acetate, and hexane) on the extraction of bioactive compounds in addition to the antioxidant activity of eight Tunisian and Algerian olive leaf ( L) cultivars. The different extracts were evaluated for their content of total phenols, flavonoids, and condensed tannins. The antioxidant capacity was determined by ABTS (2,2-azinobis (3 ethylbenzothiazoline-6-sulfonic acid) and DPPH (2,2-diphenyl-1 picrylhydrazyl) methods.

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This study delved into the incorporation of wild nettle ( L.) leaves powder with virgin olive oil, exploring its potential to enhance nutritional value and health benefits. The antioxidant, antibacterial, and mineral-enrichment attributes of the resultant nettle-infused olive oil were investigated.

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Photosynthesis, ionomics and metabolomics of the host-hemiparasite association -.

Funct Plant Biol

January 2024

Biology Department, King Khalid University, P.O. Box-9004, Abha 61413, Saudi Arabia; and Laboratory of Extremophile Plants, Biotechnology Center of Borj Cedria, University Tunis El Manar, B.P. 901, Hammam-Lif, Tunis, Tunisia.

Viscum schimperi is an evergreen hemiparasitic plant that can grow on stems and branches of several tree species. It penetrates the host tissues and forms a vascular bridge (haustorium) to withdraw the nutritive resources. Its relationships with hosts remain unknown.

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Formulating a nanoemulsion (NE) of essential oil (EO) could enhance its efficiency while requiring lower concentrations. F. Muell EO was rich in monoterpenes hydrocarbons.

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Exposure of plants to adverse environmental conditions reduces their growth and productivity. Currently, seed priming with phytohormones is considered one of the most reliable and cost-effective approaches that can help alleviate the toxic effects of environmental stress. In this context, the present study aims to investigate the effect of priming alfalfa seeds with salicylic acid (SA) on oxidative stress markers, including malonyldialdehyde, protein content, activities of antioxidant enzymes, and expression of genes encoding these enzymes in leaves and roots of alfalfa (Gabes ecotype) grown under saline stress, iron deficiency, or both.

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Combination therapy at appropriately suitable doses presents a promising alternative to monotherapeutic drugs. In this study, and essential oils and their major compounds have exhibited substantial leishmaniacidal potential against both promastigote and amastigote forms of . However, they displayed high cytotoxicity against Raw264.

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The amalgamation of garlic's antibacterial potency with olive oil's nutritional benefits provides a natural, effective way to boost health and counters microbial threats. This study explored the antioxidant and antibacterial traits of garlic-enriched virgin olive oil (VOO) samples, focusing on various garlic forms (fresh, oven-dried, freeze-dried). Comparative analysis revealed fresh garlic's highest total phenolic content, flavonoid content, and strongest DPPH scavenging activity.

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The quest for eco-friendly antifungal compounds from natural sources has surged, seeking alternatives to synthetic fungicides. In this study, we explored organic extracts antifungal potential against , a destructive fungus causing grey mold disease in crops. Extracts from various phenological stages were evaluated for their antifungal activities.

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Formulation of pasta enriched with protein-rich lupine (Lupinus mutabilis Sweet) and wheat bran using mixture design approach.

J Food Sci

October 2023

Laboratory of Materials Molecules and Applications, Preparatory Institute for Scientific and Technical Studies, Carthage University, La Marsa, Tunis, Tunisia.

Article Synopsis
  • Growing awareness of healthy diets has led to a demand for increased dietary fiber, prompting the formulation of spaghetti enriched with lupin flour and wheat bran for improved nutrition.
  • The optimal pasta recipe was created using software that analyzed the mixture, resulting in a blend of 19.60% bran, 27.83% lupin flour, and 52.75% durum wheat semolina, which enhanced the pasta's nutritional profile.
  • The enriched spaghetti showed higher protein, ash, lipids, polyphenols, and pigments, while having lower sugars, making it a better dietary choice for athletes and those on specific nutritional plans.
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Tomato pomace, composed of peels and seeds, is often discarded or used as animal feed. However, it contains valuable phytochemicals, including lycopene. Lycopene, a natural pigment, is an antioxidant known for reducing the risk of chronic diseases like cardiovascular ailments and cancer.

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  • - The study evaluated the antioxidant, antibacterial, antiviral, and anti-inflammatory properties of various parts (flowers, leaves, seeds) of a specific plant using 80% methanol extraction and microdilution techniques.
  • - Flower extracts demonstrated significant antimicrobial effectiveness against Gram-positive bacteria, as well as strong antioxidant and anti-inflammatory properties, with no toxic effects on macrophage cells.
  • - Two beneficial molecules, 12α-hydroxydaturametelin B and daturametelin B, were identified from the flower extract, showing promising potential for treating inflammatory disorders through effective binding with pro-inflammatory agents.
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In the present study, the chemical composition and the in vitro antimicrobial and antibiofilm activity of an ethanolic extract of propolis (EEP) from Tunisia against different ATCC and wild bacterial strains were evaluated. In situ antimicrobial activity and sensory influence of different EEP concentrations (0.5% and 1%), also in combination with 1% vinegar, were evaluated in chilled vacuum-packed salmon tartare.

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Anti-inflammatory activity of essential oils from Tunisian aromatic and medicinal plants and their major constituents in THP-1 macrophages.

Food Res Int

May 2023

REQUIMTE/LAQV, Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, R. Jorge Viterbo Ferreira, n° 228, 4050-313 Porto, Portugal. Electronic address:

In this study, the capacity of eight essential oils (EOs), sage (Salvia officinalis), coriander (Coriandrum sativum), rosemary (Rosmarinus officinalis), black cumin (Nigella sativa), prickly juniper (Juniperus oxycedrus), geranium (Pelargonium graveolens), oregano (Origanum vulgare) and wormwood (Artemisia herba-alba), on the inhibition of NF-κB activation was screened at concentrations up to 0.25 µL/mL using THP-1 human macrophages bearing a NF-κB reporter. This screening selected coriander, geranium, and wormwood EOs as the most active, which later evidenced the ability to decrease over 50 % IL-6, IL-1β, TNF-α and COX-2 mRNA expression in LPS-stimulated THP-1 macrophages.

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An in vitro trial was carried out to investigate the effects of natural Thymbra capitata essential oil (NEO) and its main compounds [including carvacrol, p-cymene, γ-terpinene given alone or in a synthetic combination (SEO)] on ruminal fermentation and the bacterial community using batch cultures inoculated with ruminal digesta and incubating two different basal diets [high-forage (F) and high-concentrate (C) diet]. After 24 h of incubation, primary fermentation end-products [gas, methane, volatile fatty acids (VFAs) and ammonia] and rumen microbial diversity were determined. NEO reduced the total VFA concentration (P < 0.

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NaCl effect on Cd accumulation and cell compartmentalization in barley.

Environ Sci Pollut Res Int

April 2023

Higher Institute of Arts and Crafts of Tataouine, University of Gabes, Rue Omarr Eben Khattab, 6029, Zerig-Gabes, Tunisia.

The effect of sodium chloride (NaCl) on cadmium (Cd) tolerance, uptake, translocation, and compartmentation was investigated in 3 barley genotypes. Seedlings were cultivated hydroponically in the absence of NaCl and Cd (control), in the presence of 50 mM NaCl alone, in the presence of 10 µM Cd alone, and in the combined addition of NaCl (50 mM) and Cd (10 µM). Plants were cultivated during one month under 16 h light period at a minimal light intensity of 250 µmol m s, a temperature of 25 ± 3 °C, and 70-80% of relative humidity.

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This work aims to evaluate cinnamon and curcuma essential oils as natural preservatives in minced beef meat. Essential oil chemical compositions and antibacterial activities were studied, and their encapsulation was optimized into nano-emulsions based on droplet size and distribution assessments. Selected formulas were further explored for their physical stabilities and antibacterial activities.

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This manuscript aimed to optimise the encapsulation of essential oil into nanoemulsion. Response Surface Methodology results were best fitted into polynomial models with regression coefficient values of more than 0.95.

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Nitric Oxide Metabolic Pathway in Drought-Stressed Nodules of Faba Bean ( L.).

Int J Mol Sci

October 2022

Sophia Agrobiotech Institute, INRAE 1355, CNRS 7254, Côte d'Azur University, 06903 Sophia Antipolis, France.

Drought is an environmental stress that strongly impacts plants. It affects all stages of growth and induces profound disturbances that influence all cellular functions. Legumes can establish a symbiosis with Rhizobium-type bacteria, whose function is to fix atmospheric nitrogen in organs called nodules and to meet plant nitrogen needs.

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