[Construction of recombinant Escherichia coli strains producing poly (4-hydroxybutyric acid) homopolyester from glucose].

The aim of this study is to construct recombinant Escherichia coli strains capable of producing poly(4-hydroxybutyric acid) homopolyester from glucose as sole carbon source. A glutamate: succinate semialdehyde transaminase gene from Escherichia coli, a glutamate decarboxylase gene from E. coli, and a 4-hydroxybutyrate dehydrogenase gene from Ralstonia eutropha were cloned by PCR and assembled onto the plasmid pKSSE5.