Reusable Biosensor for Easy RNA Detection from Unfiltered Saliva.

Biosensors are transforming point-of-care diagnostics by simplifying the detection process and enabling rapid, accurate testing. This study introduces a novel, reusable biosensor designed for direct viral RNA detection from unfiltered saliva, targeting SARS-CoV-2. Unlike conventional methods requiring filtration, our biosensor leverages a unique electrode design that prevents interference from saliva debris, allowing precise measurements.

Exploring the Proteomic Landscape and Immunomodulatory Functions of Derived Extracellular Vesicles.

Extracellular vesicles (EVs) have garnered attention in research for their potential as biochemical transporters and immune modulators, crucial for regulating the host immune system. The present study was conducted to isolate and characterize EVs from Gram negative bacteria (EVs) and investigate their proteomic profile and immune responses. Isolation of EVs was carried out using ultracentrifugation method.

Identification of a Biosynthetic Gene Cluster for the Production of the Blue-Green Pigment Xylindein by the Fungus .

Xylindein is a blue-green pigment produced by the fungi and Its stunning color and optoelectronic properties make xylindein valuable for textiles and as a natural semiconductor material. However, producing xylindein from culture broths remains challenging because of the slow growth of the species and the poor solubility of xylindein in organic solvents. An alternative production route for obtaining pure xylindein is heterologous expression of the xylindein biosynthetic genes.

Endophytic species from Brazil.

species can inhabit various hosts with different lifestyles and live as endophytes, pathogens, and saprobes. Our study analysed 180 endophytic isolates from sp. in the Atlantic Forest, in the Brazilian savanna (Cerrado), and in the Caatinga forest and Cerrado in Brazil.

The Genera of Fungi - G7: .

The current paper represents the seventh contribution in the Genera of Fungi series, linking type species of fungal genera to their morphology and DNA sequence data. This manuscript focuses on a genus of dematiaceous hyphomycetes, Two species of this genus are treated in this study. , the type species of the genus, as well as are epitypified and provided with DNA sequence data to resolve their phylogeny as members of (, ).

A phylogenetic assessment of in (, ): introduction of new genera, and the resurrection of .

During entomopathogenic fungal surveys conducted in Thailand, 15 specimens tentatively classified under were identified. To gain a comprehensive understanding of their taxonomy, molecular phylogenies using combined LSU, , , and sequence data, together with morphological examination of several spp. from previous studies were conducted.

Meristematic and meristematic-like fungi in .

Meristematic fungi are mainly defined as having aggregates of thick-walled, melanised cells enlarging and reproducing by isodiametric division. black meristematic and meristematic-like fungi have been allied to , which currently has two accepted families, and , with fungi mainly regarded as pathogens, parasites, saprobes and epiphytes of different plant species. This study aimed to verify the phylogenetic position using four nuclear markers (SSU, LSU, ITS and ) of the genera associated with , namely , , and , and the new genus, .

Uncovering cryptic species diversity of () associated with from Thailand.

This study advances our understanding of , an extensively studied entomopathogenic fungus within the , particularly in Thailand. We introduce seven novel species associated with - , , , , , , and . Remarkably, , , and exhibit ascomata on the subterminal region of the stromata, with the asexual form appearing at the apex of the stipe, reminiscent of .

Fungal Planet description sheets: 1697-1780.

Novel species of fungi described in this study include those from various countries as follows: , from accumulated snow sediment sample. , on leaf spots of . , on submerged decaying wood in sea water, on , as endophyte from healthy leaves of .

Environmental Variation Influences Genome Evolution in Hispaniolan Trunk Anoles (Anolis distichus).

Environmental variation often drives evolutionary processes like population differentiation, local adaptation and speciation. We used genome-scale data to investigate the contribution of environmental variation to evolution of the North Caribbean bark anole (Anolis distichus), a widespread common lizard that exhibits impressive phenotypic variation across varying habitats on the island of Hispaniola. We obtained new double-digest restriction-associated DNA sequence data (ddRADseq) from nearly 200 individuals and used 53 GIS data layers representing a range of environmental variables.

Typing of feces-derived Candida albicans strains using a novel seven-locus microsatellite panel reveals associations with yeast phenotype in individuals with inflammatory bowel disease.

Inflammatory diseases of the human gastrointestinal tract are affected by the microbes that reside in the mucosal surfaces. Patients with inflammatory bowel diseases (IBD) have altered bacterial and fungal intestinal compositions, including higher levels of fecal Candida yeasts. Ongoing research indicates that genetic and phenotypic diversity of Candida albicans may be linked with disease severity.

Superstable lipid vacuoles endow cartilage with its shape and biomechanics.

Conventionally, the size, shape, and biomechanics of cartilages are determined by their voluminous extracellular matrix. By contrast, we found that multiple murine cartilages consist of lipid-filled cells called lipochondrocytes. Despite resembling adipocytes, lipochondrocytes were molecularly distinct and produced lipids exclusively through de novo lipogenesis.

Measuring trends in extinction risk: a review of two decades of development and application of the Red List Index.

The Red List Index (RLI) is an indicator of the average extinction risk of groups of species and reflects trends in this through time. It is calculated from the number of species in each category on the IUCN Red List of Threatened Species, with trends influenced by the number moving between categories when reassessed owing to genuine improvement or deterioration in status. The global RLI is aggregated across multiple taxonomic groups and can be disaggregated to show trends for subsets of species (e.

In Vitro Antioxidant and Antibacterial Activities of Ethyl Acetate Extracts of Leaves and Five Associated Endophytic Fungi.

The exploration of new pharmacological compounds from endophytic fungi offers infinite possibilities. The aim of this study was to evaluate the antibacterial and antioxidant activities of extracts from the leaves of and five of its endophytic fungi and investigate the chemical diversity of the secondary metabolites produced. Isolated, purified, and molecularly identified endophytes and plant leaves were subjected to ethyl acetate extraction.

Taxonomical, Physiological, and Biochemical Characteristics of DSTA20 from Hypersaline Environments of Taean Salt Pond, Republic of Korea.

, a halophilic unicellular chlorophyte, produces bioactive compounds and biofuels applicable to various industries. Despite its industrial significance, comprehensive studies on the morphological, physiological, and biochemical characteristics of the genus remain challenging. In this study, we characterized an axenically isolated green alga from a salt pond in Taean, Republic of Korea, and assessed its industrially relevant traits.

Novel contribution to the taxonomy and molecular phylogeny of scuticociliates (Protozoa): Resolving the non-monophyly of the genus Paranophrys Thompson and Berger, 1965 and the establishment of the genus Anteparanophrys n. gen.

During a field survey of parasitic ciliates diversity in South Korea, a scuticociliate was found in a water sample collected during scuba diving. At first glance, the species looks similar to members of the genus Paranophrys especially P. magna but they differ mainly in the body size and the number of somatic kineties.

Biome conservatism prevailed in repeated long-distance colonization of Madagascar's mountains by Helichrysum (Compositae, Gnaphalieae).

Colonization and diversification processes are responsible for the distinctiveness of island biotas, with Madagascar standing out as abiodiversity hotspot exceptionally rich in species and endemism. Regardless of its significance, the evolutionary history and diversification drivers of Madagascar's flora remain understudied. Here we focus on Helichrysum (Compositae, Gnaphalieae) to investigate the evolutionary and biogeographic origins of the Malagasy flora.

Short communication: Exploring gastrointestinal tract nemabiomes in captive ungulates at the SANBI National Zoological Gardens: A next-generation sequencing baseline approach.

Gastrointestinal tract (GIT) nematode infections have a significant negative impact on the well-being and productivity of animals. While it is common for a host to be co-infected with multiple species of nematode parasites simultaneously, there is a lack of effective tools to study the composition of these complex parasite communities. We describe the application of the "nemabiome" amplicon sequencing to study parasitic GIT nematode communities in captive wildlife at the National Zoological Garden, South African National Biodiversity Institute.

Identification and characterization of 17 novel polynucleotide microsatellite markers in Ocypode stimpsoni (Decapoda: Ocypodidae).

Objective: The ghost crab Ocypode stimpsoni (Decapoda) is designated as a protected marine species in Korea due to its declining population. In this study, we successfully identified 17 microsatellite markers for O. stimpsoni through next-generation sequencing.

Comparative Analysis of the Biochemical and Molecular Responses of to Nitrogen and Phosphorus Limitation: Phosphorus Limitation Enhances Carotenogenesis.

is well known for its potential for biofuel production due to its high lipid content. Numerous omics and biochemical studies have explored the overall molecular mechanisms underlying the responses of sp. to nutrient availability, primarily focusing on lipid metabolism.

Exploring sp. M21F004 for Biocontrol of Bacterial and Fungal Phytopathogens.

This study explores the biocontrol potential of sp. M21F004, a lactic acid bacteria (LAB) isolated from marine environments, against several bacterial and fungal phytopathogens. Out of 50 marine bacterial isolates, sp.

Known from trees and the tropics: new insights into the species complex.

The species complex (FLSC) currently comprises 11 phylogenetic species, including accepted names such as , , and , which have mostly been reported in association with citrus and coffee. Many varieties were documented by Wollenweber & Reinking (1935), which is indicative of a wider diversity of species within this group. The lack of type material in some cases, especially for the older names, means that definition by molecular phylogeny is very difficult.

New from opposite ends of the world.

The is a group of ancient fungi with global distribution. In the current study we accessed mucoralean fungi isolated from two countries on opposite sides of the Earth and in different hemispheres: South Korea and Brazil. isolates were obtained from freshwater, soil, invertebrates, and fruit seeds and identified using phenotypic techniques combined with the DNA sequence data.

: an important genus of canker pathogens.

species have commonly been reported as important plant pathogenic fungi with wide host ranges and geographic distributions. With the increase in the number of cryptic species being described, a comprehensive global taxonomic revision of the genus is required. The present study includes 399 isolates from 32 countries.