260 results match your criteria: "Biochimie et Physique; Université du Québec à Trois-Rivières; Trois-Rivières[Affiliation]"

The detection of skeletal remains using human remain detection dogs (HRD) is often reported anecdotally by handlers to be a challenge. Limited studies have been conducted to determine the volatile organic compounds (VOCs) emitted from bones, particularly when there is limited organic matter remaining. This study aimed to determine the VOCs emitted from dry, weathered bones and examine the detection performance of HRD dogs on these bones when used as training aids.

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4-Bromo-,'-di-phenyl-benzimidamide '-oxide.

IUCrdata

October 2024

Département de Chimie, Université de Montréal, Complexe des sciences, 1375, Avenue Thérèse-Lavoie-Roux, Montréal, Québec H2V 0B3, Canada.

The title compound, CHBrNO, crystallizes with two similar mol-ecules in the asymmetric unit. The extended structure features dimers linked by pairs of N-H⋯O and C-H⋯O hydrogen bonds. The HNCNO moiety of the title compound shows delocalization over the N-C-N part, as evidenced by the similar C-N bond distances.

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ERBB2 Comprehensive Profiling and Prognostication in Stage III Colon Cancer: Findings From PETACC8 and IDEA-France Cohorts.

Gastroenterology

November 2024

Centre de Recherche des Cordeliers, Sorbonne Université, Institut National de la Santé et de la Recherche Médicale, Université Paris Cité, Personalized Medicine, Phamacogenomics and Therapeutic Optimization, Paris, France; Institut du Cancer Paris, Cancer Research for Personalized Medicine, Assistance Publique-Hôpitaux de Paris Centre, Hôpital Européen Georges Pompidou, Paris, France.

Background & Aims: ERBB2 pathway activation, through amplification or activating mutations, represents a new target for colon cancer (CC) treatment. Molecular methods were compared with the gold standard for assessing ERBB2 status, and the prognostic value of ERBB2 amplification, mutations, and expression was determined using data from 2 phase 3 trials involving nearly 3000 patients with stage III CC.

Methods: In the PETACC8 trial, immunohistochemistry and fluorescence in situ hybridization, DNA, and RNA analysis were performed on 1813, 1719, and 1733 samples, respectively.

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Biochemical, biophysical, and structural investigations of two mutants (C154Y and R312H) of the human Kir2.1 channel involved in the Andersen-Tawil syndrome.

FASEB J

November 2024

UMR 7590, CNRS, Muséum National d'Histoire Naturelle, IRD, Institut de Minéralogie, Physique des Matériaux et de Cosmochimie, IMPMC, Sorbonne Université, Paris, France.

Article Synopsis
  • Inwardly rectifying potassium (Kir) channels are essential for regulating cell resting membrane potential and repolarization in excitable cells, with Kir2.1 mutations linked to Andersen-Tawil syndrome (ATS).
  • Two specific mutations, Kir2.1-R312H and Kir2.1-C154Y, were studied in Xenopus laevis oocytes, revealing they reach the cell membrane but functionally fail to conduct potassium currents due to different molecular mechanisms.
  • The study highlights that while the WT subunit can compensate for the R312H mutation, the C154Y mutation disrupts overall channel function, pointing to structural changes that could inform future treatments for ATS.
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  • The phagocyte NADPH oxidase (NOX2) is essential for the innate immune system, producing reactive oxygen species that help destroy pathogens.
  • Researchers used circular-dichroism analyses alongside past data to assess structural models of the NADPH oxidase complex created by the AI program AlphaFold2.
  • The findings detail how specific interactions and disordered regions within proteins, particularly between p47 and cytb, play a critical role in the assembly and activation of the NADPH oxidase complex.
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Objective: Lung cancer remains the leading cause of cancer-related mortality worldwide, with most cases diagnosed at advanced stages. Hence, there is a need to develop effective predictive models for early detection. This study aims to investigate the impact of imaging parameters and delta radiomic features from temporal scans on lung cancer risk prediction.

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Rigorous field assessment in different soil types and climates comparing simulated graves with pig remains and human remains are needed to assess the capabilities and limitations of electrical resistivity tomography (ERT) as a tool to search for unmarked graves. Our study assesses the ERT signals from graves with pig and human remains in a cold, humid continental climate with sandy soils. Two sets of three experimental graves were established: the first set consisted of two graves containing human remains and an empty grave serving as a control, while the second set consisted of two graves with pig remains and a second empty grave.

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Synthesis and anticancer properties of a hybrid molecule with the testosterone and estradiol head-groups.

Steroids

September 2024

Laboratoire de Recherche en Chimie Médicinale (LRCM) et Groupe de Recherche en Signalisation Cellulaire (GRSC), Département de Chimie, Biochimie et Physique, Université du Québec à Trois-Rivières, C.P. 500, Trois-Rivières, QC, G9A 5H7, Canada. Electronic address:

This is the first report on a unique hybrid molecule made of estradiol and testosterone (TS). This distinctive hybrid molecule (1) was designed to interact with both the estrogen receptor (ER) and the androgen receptor (AR) found in hormone-dependent female and male cancer cells, and was synthesized using ethynylestradiol (17EE) as the estrogenic component and 7α-(4-azido-but-2-enyl)-4-androsten-17β-ol-3-one as the androgenic counterpart in a seven-step reaction with ∼ 26 % overall yield. We reasoned that the dual receptor binding ability could allow 1 to act as an antihormone.

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It was known that UVc irradiation increases the reactive oxygen species' (ROS) levels in bacteria hence the intervention of antioxidant enzymes and causes also changes in fatty acids (FAs) composition enabling bacteria to face antibiotics. Here, we intended to elucidate an interrelationship between SOD and susceptibility to antibiotics by studying FA membrane composition of UVc-treated P. aeruginosa PAO1 and its isogenic mutants (sodM, sodB and sod MB) membrane, after treatment with antibiotics.

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We introduce the lambda-Adaptive Biasing Force (lambda-ABF) method for the computation of alchemical free-energy differences. We propose a software implementation and showcase it on biomolecular systems. The method arises from coupling multiple-walker adaptive biasing force with λ-dynamics.

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Article Synopsis
  • Congenital myasthenic syndromes (CMS) are genetic disorders that impact neuromuscular transmission, primarily identified in childhood but often diagnosed in adulthood, leading to challenges in management.
  • A study of 235 adult CMS patients in France revealed diverse genetic mutations and highlighted the need for ongoing care, as the prognosis and long-term outcomes remain unclear.
  • The research categorized patients based on the initial symptoms and found varied disease progression patterns, with certain genotypes showing higher rates of ICU admission and the stability of phenotypical features across a patient's life.
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Enhanced structure/function of mTSPO translocator in lipid:surfactant mixed micelles.

Biochimie

September 2024

Laboratoire Léon-Brillouin (LLB), UMR12 CEA-CNRS, Université Paris-Saclay, F-91191, Gif-sur-Yvette CEDEX, France. Electronic address:

TSPO is a ubiquitous transmembrane protein used as a pharmacological marker in neuroimaging. The only known atomic structure of mammalian TSPOs comes from the solution NMR of mouse TSPO (mTSPO) bound to the PK11195 ligand and in a DPC surfactant environment. No structure is available in a biomimetic environment and without PK11195 which strongly stiffens the protein.

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Ionic liquids (ILs) have significant potential for eco-friendly extraction of uranium from aqueous solutions, which is critical for nuclear technology, fuel cycle management, and environmental protection. This study examines the impact of the adjustable hydrophobic/hydrophilic properties of ILs on the removal of uranium(VI) (UO) from aqueous solutions utilizing both a novel hydrophilic IL (1-butoxyethyl-1-methylmorpholinium butoxyethylphosphite - Mor-BOEP) and 1-heptyl-1-methylmorpholinium heptylphosphite (Mor-HP) as an example of a hydrophobic IL with a similar structure. The transfer mechanism of uranyl ions from water to organic or solid phases closely depends on the physicochemical properties of ILs, especially their hydrophobicity.

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Enhanced Electrochemical Performance in Supercapacitors through KCu-Cy Based Metal-Organic Framework Electrodes.

Chemphyschem

July 2024

Laboratoire de Recherche sur les Matériaux Alternatifs et Valorisation des Ressources, Département des sciences fondamentales, Université du Québec à Chicoutimi, 555 boulevard de l'Université, Saguenay, Chicoutimi, QC G7H 2B1, Canada.

In the realm of electronics and electric energy storage, the convergence of organic and metallic materials has yielded promising outcomes. In this study, we introduce a novel metal-organic polymer synthesized from Cyamelurate and copper (KCu-Cy) and explore its application as an electrode for a supercapacitor. This material was pressed onto a stainless-steel grid as a thin film and synthesized on nickel foam.

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Glycaemic Effects of a 156-km Ultra-trail Race in Athletes: An Observational Field Study.

Sports Med

August 2024

Univ. Lille, Univ. Artois, Univ. Littoral Côte d'Opale, ULR 7369 - URePSSS - Unité de Recherche Pluridisciplinaire Sport Santé Société, 59000, Lille, France.

Article Synopsis
  • Ultra-trail running races present significant challenges to glucose metabolism, with previous studies showing varied results based on sporadic measurements of blood sugar levels.
  • This study examined the continuous glucose levels of 55 athletes during and after a 156-km ultra-trail race, measuring factors such as running intensity, performance, and psychological stress.
  • Findings revealed no immediate risk of hypo- or hyperglycemia during the race itself, but a notable increase in hyperglycemia occurred in the 48 hours post-race, along with significant changes in various biomarkers indicative of physical stress and recovery.
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Escherichia coli CRISPR arrays from early life fecal samples preferentially target prophages.

ISME J

January 2024

Département de biochimie, de microbiologie, et de bio-informatique, Faculté des sciences et de génie, Université Laval, Québec, QC G1V 0A6, Canada.

CRISPR-Cas systems are defense mechanisms against phages and other nucleic acids that invade bacteria and archaea. In Escherichia coli, it is generally accepted that CRISPR-Cas systems are inactive in laboratory conditions due to a transcriptional repressor. In natural isolates, it has been shown that CRISPR arrays remain stable over the years and that most spacer targets (protospacers) remain unknown.

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Potential of several triazene derivatives against DENGUE viruses.

Bioorg Med Chem Lett

March 2024

Laboratoire de Chimie Organique et Thérapeutique, Faculté de Médecine, de Pharmacie et d'Odontologie de l'Université Cheikh Anta Diop de Dakar, BP 5005 Dakar-Fann, Sénégal. Electronic address:

Dengue fever is an infectious disease caused by the dengue virus (DENV), an RNA Flavivirus transmitted by the mosquitoes Aedes aegypti and Aedes albopictus widespread in tropical, subtropical and also temperate regions. Symptoms range from a simple cold to a severe, life-threatening haemorrhagic fever. According to the WHO, it affects around 390 million people per year.

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Molecular mechanisms underlying the thermal response of cells remain elusive. On the basis of the recent result that the short-time diffusive dynamics of the proteome is an excellent indicator of temperature-dependent bacterial metabolism and death, we used neutron scattering (NS) spectroscopy and molecular dynamics (MD) simulations to investigate the sub-nanosecond proteome mobility in psychro-, meso-, and hyperthermophilic bacteria over a wide temperature range. The magnitude of thermal fluctuations, measured by atomic mean square displacements, is similar among all studied bacteria at their respective thermal cell death.

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Apicomplexan parasites possess specialized secretory organelles called rhoptries, micronemes, and dense granules that play a vital role in host infection. In this study, we demonstrate that TgREMIND, a protein found in Toxoplasma gondii, is necessary for the biogenesis of rhoptries and dense granules. TgREMIND contains a Fes-CIP4 homology-Bin/Amphiphysin/Rvs (F-BAR) domain, which binds to membrane phospholipids, as well as a novel uncharacterized domain that we have named REMIND (regulator of membrane-interacting domain).

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The recent characterization of antibiotic resistance genes (ARGs) in clouds evidenced that the atmosphere actively partakes in the global spreading of antibiotic resistance worldwide. Indeed, the outdoor atmosphere continuously receives large quantities of particles of biological origins, emitted from both anthropogenic or natural sources at the near Earth's surface. Nonetheless, our understanding of the composition of the atmospheric resistome, especially at mid-altitude (i.

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AlphaFold2 (AF2) and RoseTTaFold (RF) have revolutionized structural biology, serving as highly reliable and effective methods for predicting protein structures. This article explores their impact and limitations, focusing on their integration into experimental pipelines and their application in diverse protein classes, including membrane proteins, intrinsically disordered proteins (IDPs), and oligomers. In experimental pipelines, AF2 models help X-ray crystallography in resolving the phase problem, while complementarity with mass spectrometry and NMR data enhances structure determination and protein flexibility prediction.

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Effect of Crystal Morphology on Electrochemical Performances of IRH-2 and IRH-2/PANI Composite for Supercapacitor Electrodes.

ACS Omega

November 2023

Département des sciences fondamentales, Université du Québec à Chicoutimi, 555 boulevard de l'Université, Chicoutimi, Québec G7H 2B1, Canada.

In the context of recent progress in designing metal-organic framework (MOF)-based supercapacitor electrodes, we report herein the successful growth of two different crystal morphologies of a cerium-based MOF, octahedral crystals named IRH-2-O and elongated square-bipyramidal crystals named IRH-2-ESBP (IRH = ). The identical crystal structure of both materials was confirmed by powder X-ray diffraction (PXRD). Furthermore, scanning electron microscopy and energy-dispersive X-ray mapping analysis corroborated this fact and showed the crystal shape variation the surface composition of synthesized materials.

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Chemical Characterization, DNA-Damage Protection, Antiproliferative Activity and in Silico Studies of the Essential Oils from Perralderia coronopifolia Coss.

Chem Biodivers

February 2024

Unité de Recherche: Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques, Université Frères Mentouri, Constantine 1. Route d'Aïn El Bey, 25017, Constantine, Algérie.

In this study, for the first time, we analyzed the chemical composition of essential oils (EOs) steam-distilled from the flowers and leaves of Perralderia coronopifolia by GC-FID/MS. The objective was to explore new anticancer and antioxidant bioactive substances and understand their mechanisms of action through the use of plant-derived natural products. The major chemical components characterizing the EOs were cis-chrysanthenyl acetate 1, 6-oxocyclonerolidol 2, cis-8-acetoxychrysanthenyl acetate 3, and 6α-hydroxycyclonerolidol 4, respectively.

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Dissolved metal ions and mineral-liposome hybrid systems: Underlying interactions, synthesis, and characterization.

Biochimie

December 2023

Laboratoire de Bioénergétique et Ingénierie des Protéines (BIP), Aix-Marseille Université, UMR 7281 IMM-CNRS, 31 Chemin Joseph Aiguier, 13400, Marseille, France.

Liposomes are versatile lipid-based vesicles with interesting physicochemical properties, making them excellent candidates for interdisciplinary applications in the medicinal, biological, and environmental sciences. The synthesis of mineral-liposome hybrid systems lends normally inert vesicles with the catalytic, magnetic, electrical, and optical properties of the integrated mineral species. Such applications require an understanding of the physicochemical interactions between organic molecules and inorganic crystal structures.

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