744 results match your criteria: "BioResource Research Center[Affiliation]"

The Jun dimerization protein (Jdp2) gene is active in mouse cerebellar granule cells and its protein product plays a crucial role in the formation of the cerebellum lobes through programmed cell death. However, the role of Jdp2 in cellular differentiation and pluripotency in the cerebellum, and the effect of the antioxidation reaction on cell plasticity, remain unknown. N-acetyl-L-cysteine (NAC) induced the early commitment of the differentiation of granule cell precursors (GCPs) to neurons, especially Purkinje cells, via the γ-aminobutyric acid type A receptor α6 subunit (Gabra6) axis; moreover, Jdp2 depletion enhanced this differentiation program of GCPs.

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Unveiling three novel actinobacterial species ( sp. nov., sp. nov. and sp. nov.) in soil samples in Phu Pha Phet Cave, Thailand.

Int J Syst Evol Microbiol

December 2024

Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, 254 Phayathai Road, Wangmai, Pathumwan, Bangkok 10330, Thailand.

Three strains of actinobacteria, designated as B-S-A6, B-S-A8 and B-S-A12, were isolated from soil samples collected in the Phu Pha Phet Cave located in the Satun UNESCO Global Geopark, Satun Province, Thailand. A comprehensive polyphasic approach was used to describe these strains. Strains B-S-A6, B-S-A8 and B-S-A12 were identified within the genus based on a comparative examination of 16S rRNA gene sequences.

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sp. nov., a new member of the phylum , from forest soil in a geothermal area.

Int J Syst Evol Microbiol

December 2024

Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Kampus UI, Depok 16424, Indonesia.

Three Gram-stain-positive aerobic bacteria, characterized by branched mycelia with putative sporangia, were isolated from forest soil inside a decayed bamboo stem from a geothermal area in West Java, Indonesia. The strain S3.2.

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Background/aim: Chemoresistance to paclitaxel (PTX) significantly ameliorates therapeutic efficacy in patients with non-small cell lung cancer (NSCLC), especially in advanced stages, deteriorating the progression free and overall survival rates. One of the critical mechanisms contributing to drug resistance is the excretion of PTX from target cells via efflux pumps. Ivermectin was developed as a bactericidal agent against parasites; however, it has recently been shown to inhibit the proliferation of human cancer cells.

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Striosome circuitry stimulation inhibits striatal dopamine release and locomotion.

J Neurosci

December 2024

Institute for Advancement of Clinical and Translational Science (iACT), Kyoto University Hospital, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan

The mammalian striatum is divided into two types of anatomical structures: the island-like, mu opioid receptor (MOR)-rich striosome compartment and the surrounding matrix compartment. Both compartments have two types of spiny projection neurons (SPNs), dopamine receptor D1 (D1R)-expressing direct pathway SPNs (dSPNs) and dopamine receptor D2 (D2R)-expressing indirect pathway SPNs. These compartmentalized structures have distinct roles in the development of movement disorders, although the functional significance of the striosome compartment for motor control and dopamine regulation remains to be elucidated.

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Three yeast strains were isolated from an olive paste sample (CBS 18661) and two samples of olive oil (CBS 18662 and CBS 18660) were collected in two different mills in Verona province, Italy. The sequence comparison of the D1/D2 domains of the LSU rRNA gene and the internal transcribed space (ITS) regions indicated that these strains belonged to the genus . Phylogenetic analysis of concatenated sequences of the ITS regions and the D1/D2 domains indicated that they represented members of two distinct species.

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Generation of human induced pluripotent stem cell lines derived from patients of cystic biliary atresia.

Hum Cell

November 2024

iPS Cell Advanced Characterization and Development Team, BioResource Research Center, RIKEN, 3-1-1 Koyadai, Tsukuba, Ibaraki, 305-0074, Japan.

Article Synopsis
  • Biliary atresia (BA) is a serious liver condition caused by abnormal development of bile ducts, leading to liver damage and possible cirrhosis; type I cystic BA is a rare but important form of this condition.
  • Researchers have created a set of human induced pluripotent stem cells (hiPSCs) from five Japanese patients with type I cystic BA, which show potential for self-renewal and differentiation.
  • Whole genome analysis revealed mutations linked to hepatobiliary issues in these patients, and studying these hiPSCs may help improve understanding of the disease and lead to new treatments.
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  • HRV44, a thermophilic chemolithoautotroph, is highly effective at converting nitrous oxide (NO) to dinitrogen gas and possesses a specific type of enzyme (NosZ) involved in this reduction process.
  • Transcriptome and proteomic analyses showed that many genes related to denitrification were active even without the presence of nitrogen oxides, suggesting a baseline readiness for NO reduction.
  • The study found that gene expression involved in electron transport to NosZ increased in response to NO within three hours, while two genes regulating this process showed negative correlations with their expression, indicating complex regulatory interactions.
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GCM and gcType in 2024: comprehensive resources for microbial strains and genomic data.

Nucleic Acids Res

November 2024

Microbial Resource and Big Data Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.

Microbial culture collections play a crucial role in the collection, maintenance, and distribution of quality-assured living microbial strains, along with their associated phenotypic and omics data. To enhance the find-able, accessible, interoperable, and re-usable (FAIR) data utilization of microbial resources, the World Data Center for Microorganisms (WDCM) has developed the Global Catalogue of Microorganisms (GCM) and the Global Catalogue of Type Strains (gcType). These platforms provide interactive interfaces for cataloging the holdings of collections, along with detailed annotations of type strain genomes and curated metadata, including ecosystems, growth conditions, and collection locations.

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Generation of human induced pluripotent stem cell lines derived from two glucose transporter 1 deficiency syndrome patients.

Stem Cell Res

December 2024

iPS Cell Advanced Characterization and Development Team, BioResource Research Center, RIKEN, 3-1-1 Koyadai, Tsukuba, Ibaraki 305-0074, Japan; School of Integrative and Global Majors, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan. Electronic address:

Glucose transporter 1 deficiency syndrome (GLUT1DS), caused by impaired glucose transport at the blood-brain barriers, leads to various central nervous system dysfunctions. A comprehensive understanding of the underlying disease pathogenesis is still lacking. In this study, we have generated GLUT1DS-specific human induced pluripotent stem cells (hiPSCs) derived from two patients.

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strain m318 is an arsenite-oxidizing rhizobacterium isolated from the rhizosphere of an arsenic hyperaccumulator plant, beneficial for arsenic phytoremediation. Here, we report the complete genome sequence of this strain, which consists of a circular chromosome assembled using long reads sequenced on Nanopore and polished with Illumina paired-end reads.

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SSBD: an ecosystem for enhanced sharing and reuse of bioimaging data.

Nucleic Acids Res

October 2024

Laboratory for Developmental Dynamics, RIKEN Center for Biosystems Dynamics Research, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan.

Article Synopsis
  • SSBD is a platform designed for sharing and reusing bioimaging data, recently updated to include a public repository (SSBD:repository) for all types of bioimaging data and a curated database (SSBD:database) for highly reusable data.
  • This two-tiered system balances the need for quick data publication with the demand for well-annotated, rich metadata, enhancing the ability to share and reuse bioimaging data.
  • SSBD is becoming a vital part of the international consortium foundingGIDE, which aims to create a global ecosystem for image data, allowing for improved data exchange and cross-searching capabilities across different regions.
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Recent studies have revealed that biotic interactions in ecological communities vary over time, possibly mediating community responses to anthropogenic disturbances. This study investigated the heterogeneity of such variability within a real community and its impact on population stability in the face of pesticide application, particularly focusing on density-dependence of the interaction effect. Using outdoor mesocosms with a freshwater community, we found considerable heterogeneity in density-dependent interaction variability among links in the same community.

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Gene editing in human induced pluripotent stem (iPS) cells with programmable nucleases facilitates reliable disease models, but methods using double-strand break repair often produce random on-target by-products. Prime editing (PE) combines Cas9 nickase with reverse transcriptase and PE guide RNA (pegRNA) encoding a repair template to reduce by-products. We implemented a GMP-compatible protocol for transfecting Cas9- or PE-2A-mCherry plasmids to track and fractionate human iPS cells based on PE expression level.

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Article Synopsis
  • Plants use pattern recognition receptors (PRRs) like EFR and FLS2 to detect bacterial pathogens and activate their immune response via RBOHD.
  • QSK1, identified as a protein associated with the PRR-RBOHD complex, acts as a negative regulator by downregulating EFR and FLS2, leading to suppressed immunity.
  • The bacterial effector HopF2Pto manipulates QSK1 to inhibit immune responses, demonstrating the sophisticated interplay between plant defense mechanisms and pathogen strategies.
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Deep-sea massive sulfide deposits serve as energy sources for chemosynthetic ecosystems in dark, cold environments even after hydrothermal activity ceases. However, the vertical distribution of microbial communities within sulfide deposits along their depth from the seafloor as well as their ecological roles remain unclear. We herein conducted a culture-independent metagenomic ana-lysis of a core sample of massive sulfide deposits collected in a hydrothermally inactive field of the Southern Mariana Trough, Western Pacific, by drilling (sample depth: 0.

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Background: Embryonic craniofacial development involves several cellular and molecular events that are evolutionarily conserved among vertebrates. Vertebrate models such as mice and zebrafish have been used to investigate the molecular and cellular etiologies underlying human craniofacial disorders, including orofacial clefts. However, the molecular mechanisms underlying embryonic development in these two species are unknown.

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Paracraurococcus lichenis sp. nov., isolated from lichen in Thailand.

Arch Microbiol

September 2024

Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, 254 Phayathai Road, Wangmai, Bangkok, 10330, Thailand.

A novel bacterium, designated as strain LOR1-02 and isolated from a lichen sample collected from Kham Riang Subdistrict, Kantharawichai District, Maha Sarakham Province, Thailand, underwent thorough investigation utilizing a polyphasic taxonomic approach. Strain LOR1-02 demonstrated growth within a temperature range of 20-42 °C (optimal at 30 °C), pH range of 5.0-7.

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Introduction: is a strong tumor suppressor gene; its deactivation contributes to carcinogenesis and influences clinical outcomes. However, the prognostic influence of p53 deactivation on early relapse in patients with surgically resected non-small cell lung cancer remains unclear.

Materials And Methods: A cohort of 170 patients with primary stage I through III lung adenocarcinoma (LADC) and lung squamous cell carcinoma who underwent complete resection at Tokyo Medical and Dental University was screened for mutations using panel testing, and association studies between mutations and clinical data, including histology and postoperative recurrence, were performed.

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