43 results match your criteria: "Beltsville Agricultural Research Center (BARC)[Affiliation]"
Transl Anim Sci
September 2024
Metabolite Profiling Facility, Bindley Bioscience Center, Purdue University, West Lafayette, IN 47907, USA.
Multiple reaction monitoring (MRM) profiling is a sensitive method of lipid screening that has the capability to distinguish between different fertility phenotypes in gilts. However, MRM profiling has not yet been utilized to evaluate fertility phenotypes in boars. Markers indicative of fertility status in boars would be valuable as inclusion of subfertile boars in breeding programs results in a loss of efficiency and negative economic consequences.
View Article and Find Full Text PDFAnimals (Basel)
October 2024
Animal Biosciences and Biotechnology Laboratory (ABBL), Beltsville Agricultural Research Center (BARC), United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Beltsville, MD 20705, USA.
Coccidiosis in broiler chickens continues to be a major disease of the gastrointestinal tract, causing economic losses to the poultry industry worldwide. The goal of this study was to generate a symptomatic (1000 oocysts) infection to determine its effect on the luminal and mucosal microbiota populations (L and M) in the jejunum and ileum (J and IL). Samples were taken from day 0 to 14 post-infection, and sequencing of 16S rRNA was performed using Illumina technology.
View Article and Find Full Text PDFHortic Res
April 2024
Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT 06269, USA.
Long-distance transport or systemic silencing effects of exogenous biologically active RNA molecules in higher plants have not been reported. Here, we report that cationized bovine serum albumin (cBSA) avidly binds double-stranded beta-glucuronidase RNA (dsGUS RNA) to form nucleic acid-protein nanocomplexes. In our experiments with tobacco and poplar plants, we have successfully demonstrated systemic gene silencing effects of cBSA/dsGUS RNA nanocomplexes when we locally applied the nanocomplexes from the basal ends of leaf petioles or shoots.
View Article and Find Full Text PDFInt J Mol Sci
July 2024
Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
Mammalian spermatozoa rely on glycolysis and mitochondrial oxidative phosphorylation for energy leading up to fertilization. Sperm capacitation involves a series of well-regulated biochemical steps that are necessary to give spermatozoa the ability to fertilize the oocyte. Additionally, zinc ion (Zn) fluxes have recently been shown to occur during mammalian sperm capacitation.
View Article and Find Full Text PDFTransl Anim Sci
April 2024
Department of Animal Science, Purdue University, West Lafayette, IN 47907, USA.
Zinc () supplementation has proved to mitigate the effects of heat stress with varying effects evident with Zn source during acute heat events. However, the effects of Zn supplementation during long-term summer weather patterns have yet to be explored. Therefore, the objective of this study was to identify the effects of supplementation source and level of Zn to mitigate the negative effects of long-term, cyclic heat stress in finishing swine.
View Article and Find Full Text PDFBiol Open
August 2023
Department of Animal Science, Purdue University, West Lafayette, IN 47907, USA.
Low colostrum intake relates to poorer health and infertility in swine. We previously connected vaginal lipid profiles at weaning to fertility of sows. We hypothesized vaginal lipidome varied with colostrum intake.
View Article and Find Full Text PDFTransgenic Res
April 2023
Simplot Plant Sciences, J. R. Simplot Company, Boise, ID, 83706, USA.
Phytophthora infestans, the etiologic agent of late blight, is a threat to potato production in areas with high humidity during the growing season. The oomycete pathogen is hemi-biotrophic, it establishes infection on living plant cells and then spreads, kills, and feeds off the necrotized plant tissue material. The interaction between host and pathogen is complex with dynamic pathogen RXLR effectors and potato NB-LRR resistance proteins actively competing for dominance and survival.
View Article and Find Full Text PDFHortic Res
February 2023
Department of Horticultural Science, North Carolina State University, Raleigh, NC 27607, USA.
Hortic Res
March 2022
Department of Horticultural Science, North Carolina State University, Raleigh, NC, 27607, USA.
Plant transformation and regeneration remain highly species- and genotype-dependent. Conventional hormone-based plant regeneration via somatic embryogenesis or organogenesis is tedious, time-consuming, and requires specialized skills and experience. Over the last 40 years, significant advances have been made to elucidate the molecular mechanisms underlying embryogenesis and organogenesis.
View Article and Find Full Text PDFFront Immunol
March 2022
Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center (BARC), Agricultural Research Service (ARS), United States Department of Agriculture (USDA), Beltsville, MD, United States.
Current research efforts require a broad range of immune reagents, but those available for pigs are limited. The goal of this study was to generate priority immune reagents for pigs and pipeline them for marketing. Our efforts were aimed at the expression of soluble swine cytokines and the production of panels of monoclonal antibodies (mAbs) to these proteins.
View Article and Find Full Text PDFFront Immunol
March 2022
Animal Parasitic Disease Laboratory, Beltsville Agricultural Research Center (BARC) Agricultural Research Service (ARS), United States Department of Agriculture (USDA), Beltsville, MD, United States.
Natural killer (NK) cells have been studied extensively in humans and mice for their vital role in the vertebrate innate immune system. They are known to rapidly eliminate tumors or virus infected cells in an immune response utilizing their lytic properties. The natural cytotoxicity receptors (NCRs) NKp30 (NCR3), NKp44 (NCR2), and NKp46 (NCR1) are important mediators of NK-cell cytotoxicity.
View Article and Find Full Text PDFJ Anim Sci
February 2022
USDA ARS US Meat Animal Research Center (USMARC), Clay Center, NE 68933, USA.
Biol Reprod
April 2022
Department of Animal Science, Michigan State University, East Lansing, MI, USA.
Increased knowledge of reproduction and health of domesticated animals is integral to sustain and improve global competitiveness of U.S. animal agriculture, understand and resolve complex animal and human diseases, and advance fundamental research in sciences that are critical to understanding mechanisms of action and identifying future targets for interventions.
View Article and Find Full Text PDFFront Vet Sci
August 2021
Centro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, Colombia.
is frequently reported in fecal samples from animals and humans worldwide, and a variety of subtypes (STs) have been observed in wild and domestic animals. In Colombia, few studies have focused on the transmission dynamics and epidemiological importance of in animals. In this study, we characterized the frequency and subtypes of in fecal samples of domestic animals including pigs, minipigs, cows, dogs, horses, goats, sheep, and llama from three departments of Colombia.
View Article and Find Full Text PDFSci Rep
August 2021
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand.
Host-specific legume-rhizobium symbiosis is strictly controlled by rhizobial type III effectors (T3Es) in some cases. Here, we demonstrated that the symbiosis of Vigna radiata (mung bean) with Bradyrhizobium diazoefficiens USDA110 is determined by NopE, and this symbiosis is highly dependent on host genotype. NopE specifically triggered incompatibility with V.
View Article and Find Full Text PDFFront Genet
March 2021
Department of Animal and Avian Sciences, University of Maryland, College Park, MD, United States.
Low and high egg producing hens exhibit gene expression differences related to ovarian steroidogenesis. High egg producing hens display increased expression of genes involved in progesterone and estradiol production, in the granulosa layer of the largest follicle (F1G) and small white follicles (SWF), respectively, whereas low egg producing hens display increased expression of genes related to progesterone and androgen production in the granulosa (F5G) and theca interna layer (F5I) of the fifth largest follicle, respectively. Transcriptome analysis was performed on F1G, F5G, F5I, and SWF samples from low and high egg producing hens to identify novel regulators of ovarian steroidogenesis.
View Article and Find Full Text PDFJ Nematol
January 2020
USDA ARS Mycology and Nematology Genetic Diversity and Biology Laboratory , Northeast Area , Henry A. Wallace Beltsville Agricultural Research Center (BARC)-West, Beltsville, MD, 20705.
hybrids are forage grasses used worldwide in temperate climates. They are associated with the fungal endophyte , which aids in nutrient uptake, drought tolerance, and production of metabolites that protect against parasites and herbivores. produces loline alkaloids, which can deter insect pests.
View Article and Find Full Text PDFSci Total Environ
May 2020
US Department of Agriculture (USDA), Agricultural Research Service (ARS), Beltsville Agricultural Research Center (BARC), 10300 Baltimore Avenue, Beltsville, MD 20705, USA.
Nitrogen pollution in watersheds containing significant cropland area is generally problematic. Conservation practices intended to reduce nitrate-N (NO-N) export from watersheds are being implemented by many regions without necessary tools to assess effectiveness of abatement. A commonly used herbicide metolachlor degrades in the vadose zone of croplands to form two metabolites (metolachlor ethane sulfonic acid (MESA) and metolachlor oxanilic acid (MOXA)) which are both highly soluble in soils.
View Article and Find Full Text PDFJ Agric Food Chem
February 2020
Pasture Systems & Watershed Management Research , US Department of Agriculture (USDA), Agricultural Research Service (ARS) , Curtin Road, Building 3702 , University Park, Pennsylvania 16802 , United States.
Avian Dis
September 2019
Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center (BARC), Agricultural Research Service (ARS), U.S. Department of Agriculture (USDA), Beltsville, MD 20705,
(CP) type A and newly created type G strains are the key etiological factors in the induction of necrotic enteritis (NE), an important enteric disease that is responsible for the annual loss of $6 billion in the worldwide poultry industry. Several CP toxin genes were found to be critical in NE pathogenesis in chickens, but limited information is available on the CP lethal toxin gene. In this study, 19 CP strains isolated from NE-affected chicken farms were characterized microbiologically and molecularly and evaluated for their pathogenicity in commercial broiler chickens.
View Article and Find Full Text PDFVet Parasitol
May 2019
United States Department of Agriculture (USDA), Agricultural Research Service (ARS), Beltsville Agricultural Research Center (BARC), Animal Parasitic Diseases Laboratory, Beltsville, MD 20705, USA. Electronic address:
Among the meat sources of Toxoplasma gondii, pork is considered important in the epidemiology of toxoplasmosis in the USA. How soon after infection T. gondii forms tissue cysts in pork is unknown.
View Article and Find Full Text PDFRev Bras Parasitol Vet
July 2019
Departamento de Ciências Veterinárias, Escola de Ciências Agrárias e Veterinárias, Universidade de Trás-os-Montes e Alto Douro - UTAD, Vila Real, Portugal.
Neospora spp. are intracellular protozoa with worldwide distribution and closely related to Toxoplasma gondii, which can infect a variety of mammals including horses. From September 2013 to June 2014, 185 horses from northern, central and southern parts of mainland Portugal were randomly sampled and tested for detection of immunoglobulin (Ig) G antibodies to Neospora spp.
View Article and Find Full Text PDFJuvenile, female and male nematodes were discovered in wood chips of white pine from Ashley Falls, MA. Initial observations suggested these nematodes might be PWN, but closer morphological and molecular characterization proved otherwise. Comparison of measured features with those in the literature indicated this nematode population had some unique characteristics.
View Article and Find Full Text PDFJ Nematol
March 2016
USDA, ARS, Nematology Laboratory, Northeast Area, Henry A. Wallace Beltsville Agricultural Research Center (BARC)-West, Beltsville, MD 20705.
Pseudomonas fluorescens isolates Clinto 1R, Wayne 1R, and Wood 1R, which produce the antibiotic 2,4-diacetylphloroglucinol (DAPG), can suppress soilborne diseases and promote plant growth. Consequently, these beneficial bacterial isolates were tested on watermelon plants for suppression of Meloidogyne incognita (root-knot nematode: RKN) and Fusarium oxysporum f. sp.
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