, , and identified as antibiotic-producers from freshwater.

Three water isolates were previously identified as promising antibiotic producers from freshwater sources in Wisconsin, United States. Each isolate produced effective antibiotics against three or more bacterial relatives of antibiotic resistant pathogens. The isolates were identified as , , and through 16S rRNA sequencing and further characterized with biochemical tests to verify the genus and species of each isolate.

Seventeen antibiotic-producing bacteria isolates found across four freshwater environments.

Antibiotics are produced by microorganisms as defense mechanisms against bacteria and have treated bacterial infections for decades. Most of the current antibiotics are extracted from soil bacteria, and no new antibiotic class has been found in nearly 40 years. However, antibiotic-producing bacteria were discovered on tree bark, emphasizing that other environments should be explored for these bacteria.

A new paradigm in intracellular immunology: Mitochondria emerging as leading immune organelles.

Mitochondria, traditionally recognized as cellular 'powerhouses' due to their pivotal role in energy production, have emerged as multifunctional organelles at the intersection of bioenergetics, metabolic signaling, and immunity. However, the understanding of their exact contributions to immunity and inflammation is still developing. This review first introduces the innovative concept of intracellular immunity, emphasizing how mitochondria serve as critical immune signaling hubs.

Five tree species contained antibiotic-producing bacteria within their bark.

Soil is a common source for identifying antibiotic-producing bacteria; however, other ecosystems in nature may contain novel bacteria capable of producing antibiotics. Bark from seven tree species was collected as a new source to culture bacterial isolates that were screened against nine tester bacteria related to antibiotic resistant pathogens. Five of the seven tree species contained isolates that showed antibiotic production against at least one of the tester bacteria.

Innate immunity of vascular smooth muscle cells contributes to two-wave inflammation in atherosclerosis, twin-peak inflammation in aortic aneurysms and trans-differentiation potential into 25 cell types.

Introduction: Vascular smooth muscle cells (VSMCs) are the predominant cell type in the medial layer of the aorta, which plays a critical role in aortic diseases. Innate immunity is the main driving force for cardiovascular diseases.

Methods: To determine the roles of innate immunity in VSMC and aortic pathologies, we performed transcriptome analyses on aortas from ApoE angiotensin II (Ang II)-induced aortic aneurysm (AAA) time course, and ApoE atherosclerosis time course, as well as VSMCs stimulated with danger-associated molecular patterns (DAMPs).

Nonheme Fe═O Complexes Supported by Four Pentadentate Ligands: Reactivity toward H- and O- Atom Transfer Processes.

Four new pentadentate N5-donor ligands, [-(1-methyl-2-imidazolyl)methyl--(2-pyridyl)-methyl--(bis-2-pyridylmethyl)-amine] (), [-bis(1-methyl-2-imidazolyl)methyl--(bis-2-pyridylmethyl)amine] (), (-(isoquinolin-3-ylmethyl)-1,1-di(pyridin-2-yl)--(pyridin-2-ylmethyl)methanamine (), and ,-bis(isoquinolin-3-ylmethyl)-1,1-di(pyridin-2-yl)methanamine (), have been synthesized based on the N4Py ligand framework, where one or two pyridyl arms of the N4Py parent are replaced by (-methyl)imidazolyl or -(isoquinolin-3-ylmethyl) moieties. Using these four pentadentate ligands, the mononuclear complexes [Fe(CHCN)()] (), [Fe(CHCN)()] (), [Fe(CHCN)()] (), and [Fe(CHCN)()] () have been synthesized and characterized. The half-wave potentials () of the complexes become more positive in the order: < < ≤ ≤ [Fe(N4Py)(CHCN)].

Synthesis of phosphine derivatives of [Fe(CO)(μ-sdt)] (sdt = SCHSCHS) and investigation of their proton reduction capabilities.

The reactions of [Fe(CO)(μ-sdt)] (1) (sdt = SCHSCHS) with phosphine ligands have been investigated. Treatment of 1 with dppm (bis(diphenylphosphino)methane) or dcpm (bis(dicyclohexylphosphino)methane) affords the diphosphine-bridged products [Fe(CO)(μ-sdt)(μ-dppm)] (2) and [Fe(CO)(μ-sdt)(μ-dcpm)] (3), respectively. The complex [Fe(CO)(μ-sdt)(κ-dppv)] (4) with a chelating diphosphine was obtained by reacting 1 with dppv (cis-1,2-bis(diphenylphosphino)ethene).

Adaptive Stimulations in a Biophysical Network Model of Parkinson's Disease.

Deep brain stimulation (DBS)-through a surgically implanted electrode to the subthalamic nucleus (STN)-has become a widely used therapeutic option for the treatment of Parkinson's disease and other neurological disorders. The standard conventional high-frequency stimulation (HF) that is currently used has several drawbacks. To overcome the limitations of HF, researchers have been developing closed-loop and demand-controlled, adaptive stimulation protocols wherein the amount of current that is delivered is turned on and off in real-time in accordance with a biophysical signal.

A portable x-ray fluorescence (pXRF) elemental dataset collected from Cambrian-age sandstone aquifer material, Wisconsin, U.S.A.

This article provides a portable x-ray fluorescence (pXRF) elemental dataset from samples collected from a Cambrian Sandstone Aquifer in West-Central Wisconsin, U.S.A.

Carving Up Participation: Sense-Making and Sociomorphing for Artificial Minds.

AI (broadly speaking) as a discipline and practice has tended to misconstrue social cognition by failing to properly appreciate the role and structure of the interaction itself. Participatory Sense-Making (PSM) offers a new level of description in understanding the potential role of (particularly robotics-based) AGI in a social interaction process. Where it falls short in distinguishing genuine living sense-makers from potentially cognitive artificial systems, sociomorphing allows for gradations in how these potential systems are defined and incorporated into asymmetrical sociality.

Evolutionary Genome Mining for the Discovery and Engineering of Natural Product Biosynthesis.

Genome mining has become an invaluable tool in natural products research to quickly identify and characterize the biosynthetic pathways that assemble secondary or specialized metabolites. Recently, evolutionary principles have been incorporated into genome mining strategies in an effort to better assess and prioritize novelty and understand their chemical diversification for engineering purposes. Here, we provide an introduction to the principles underlying evolutionary genome mining, including bioinformatic strategies and natural product biosynthetic databases.

Identifying the source of groundwater contaminants in West-Central Wisconsin, U.S.A.: Geochemical and mineralogical characterization of the Cambrian sandstone aquifer.

Naturally-sourced groundwater quality issues are common, but there is rarely a geochemical and mineralogical dataset of aquifer properties for comparison. This study utilizes geochemical and mineralogical data to characterize naturally-occurring minerals that are potential groundwater contaminants in the Cambrian Eau Claire - Mount Simon aquitard-aquifer system of west-central Wisconsin, U.S.

Sukhareva's (1930) 'Toward the problem of the structure and dynamics of children's constitutional psychopathies (Schizoid forms)': a translation with commentary.

This article constitutes a translation from the Russian of G.E. Sukhareva's research report entitled (in English) 'Toward the problem of the structure and dynamics of children's constitutional psychopathies (Schizoid forms)', which she delivered at the 'behavioral' conference in Leningrad in early 1930.

Two distinct classes of cochaperones compete for the EEVD motif in heat shock protein 70 to tune its chaperone activities.

Chaperones of the heat shock protein 70 (Hsp70) family engage in protein-protein interactions with many cochaperones. One "hotspot" for cochaperone binding is the EEVD motif, found at the extreme C terminus of cytoplasmic Hsp70s. This motif is known to bind tetratricopeptide repeat domain cochaperones, such as the E3 ubiquitin ligase CHIP.

Larval Exposure to Polychlorinated Biphenyl-126 Led to a Long-Lasting Decrease in Immune Function in Postmetamorphic Juvenile Northern Leopard Frogs, Lithobates pipiens.

Amphibian populations are decreasing worldwide, and pollution is a contributing factor. Polychlorinated biphenyls (PCBs) are a class of persistent organic pollutants known to exert immunotoxicity. To assess impacts of PCBs on frogs, we exposed Lithobates pipiens tadpoles to a diet of PCB-126 (0-5 ng PCB-126/g wet food) through metamorphic climax.

Culturally Grounded Scapegoating in Response to Illness and the COVID-19 Pandemic.

For years, violence against doctors and healthcare workers has been a growing social issue in China. In a recent series of studies, we provided evidence for a motivated scapegoating account of this violence. Specifically, individuals who feel that the course of their (or their family member's) illness is a threat to their sense of control are more likely to express motivation to aggress against healthcare providers.

Essential or not? Knowledge problems and COVID-19 stay-at-home orders.

In response to the COVID-19 pandemic, governments around the world issued stay-at-home orders, which required that individuals stay at home unless they were engaging in certain activities. Often these orders would designate certain goods and services as "essential" and would permit individuals engaged in the production, delivery, and purchase of those goods and services to leave their homes to do so. Implicit in these policies, of course, is the assumption that policymakers can know which goods and services are essential.

Growth and sexual dimorphism of the hyoid bone and its relationship to the mandible from birth to 19 years: A three-dimensional computed tomography study.

The hyoid bone and the hyomandibular complex subserve the functions of respiration, deglutition, and speech. This study quantified the growth of the hyoid bone and the hyomandibular relationships in males and females from birth to 19 years. Using 97 computed tomography (CT) scans, from a previous study (Kelly et al.

Making meaning of motherhood: Self and life transitions among African American adolescent mothers.

Although adolescent childbearing is widely viewed as a major social problem, and research suggests that young mothers and their children are at risk for poor economic and academic outcomes, these ideas may be in need of revision. Recent scholarship has pointed out that young mothers have been unfairly blamed and stigmatized for problems that should be attributed to social and economic inequality, racism, and poverty. The present study extends that research by listening to the voices of young mothers.

Electrocatalytic proton-reduction behaviour of telluride-capped triiron clusters: tuning of overpotentials and stabilization of redox states relative to lighter chalcogenide analogues.

Reaction of [Fe3(CO)9(μ3-Te)2] (1) with the corresponding phosphine has been used to prepare the phosphine-substituted tellurium-capped triiron clusters [Fe3(CO)9(μ3-Te)2(PPh3)] (2), [Fe3(CO)8(μ3-Te)2(PPh3)] (3) and [Fe3(CO)7(μ3-Te)2(μ-R2PXPR2)] (X = CH2, R = Ph (4), Cy (5); X = NPri, R = Ph (6)). The directly related cluster [Fe3(CO)7(μ3-CO)(μ3-Te)(μ-dppm)] (7) was isolated from the reaction of [Fe3(CO)10(μ-Ph2PCH2PPh2)] with elemental tellurium. The electrochemistry of these new clusters has been probed by cyclic voltammetry, and selected complexes have been tested as proton reduction catalysts.

Resources for Teaching and Assessing the Biology Core Concepts.

The report called for the biology community to mobilize around teaching the core concepts of biology. This essay describes a collection of resources developed by several different groups that can be used to respond to the report's call to transform undergraduate education at both the individual course and departmental levels. First, we present two frameworks that help articulate the core concepts, the and the Conceptual Elements (CE) Framework, which can be used in mapping the core concepts onto existing curricula and designing new curricula that teach the biology core concepts.

Shared transcriptional responses to con- and heterospecific behavioral antagonists in a wild songbird.

The recognition of and differential responses to salient stimuli are among the main drivers of behavioral plasticity, yet, how animals evolve and modulate functional responses to novel classes of antagonistic stimuli remain poorly understood. We studied free-living male red-winged blackbirds (Agelaius phoeniceus) to test whether gene expression responses in blood are distinct or shared between patterns of aggressive behavioral responses directed at simulated conspecific versus heterospecific intruders. In this species, males defend territories against conspecific males and respond aggressively to female brown-headed cowbirds (Molothrus ater), a brood parasite that commonly lays eggs in blackbird nests.

Undergraduate biological sciences and biotechnology students' reflective essays focus on descriptive details of experiential learning experiences.

Experiential learning experiences (ELEs), opportunities for students to apply knowledge and skills critically in a hands-on environment, are fundamental to the apprenticeship model of biological and biotechnological sciences. ELEs enhance student-learning gains, increase career readiness, and provide important networking opportunities. However, students do not often recognize the benefits of ELEs.

A Biology Core Concept Instrument (BCCI) to Teach and Assess Student Conceptual Understanding.

Instruments for teaching and assessing student understanding of the five core concepts in biology from are needed. We developed four Biology Core -Concept Instruments (BCCIs) that teach and assess students' ability to describe a concept in their own words, identify concepts represented in biological phenomena, and make connections between concepts. The BCCI includes a narrative, followed by a series of 10 true-false/identify (TF/I) and three open-ended questions.

Inclusive practices for diverse student populations: Experimental Biology 2017.

As student populations become more diverse, it is essential for educators, administrators, and institutions to implement practices that ensure the success of all students. This is particularly true in the sciences, as students from traditionally underrepresented populations in STEM compose an increasingly greater proportion of the national student demographic. The Teaching Section of the American Physiological Society sponsored a symposium, "Inclusive Practices for Diverse Student Populations," at 2017 Experimental Biology in Chicago, IL, introducing practices that promote inclusion in diverse student populations in STEM.