Biodiversity within phytoplankton-associated microbiomes regulates host physiology, host community ecology, and nutrient cycling.

Unlabelled: Biological diversity is declining across the tree of life, including among prokaryotes. With the increasing awareness of host-associated microbes as potential regulators of eukaryotic host physiology, behavior, and ecology, it is important to understand the implications of declining diversity within host microbiomes on host fitness, ecology, and ecosystem function. We used phytoplankton and their associated environmental microbiomes as model systems to test the independent and interactive effects of declining microbiome diversity with and without other stressors often caused by human activity-elevated temperature and altered nutrient availability.

Comparison of different microbiome analysis pipelines to validate their reproducibility of gastric mucosal microbiome composition.

Microbiome analysis has become a crucial tool for basic and translational research due to its potential for translation into clinical practice. However, there is ongoing controversy regarding the comparability of different bioinformatic analysis platforms and a lack of recognized standards, which might have an impact on the translational potential of results. This study investigates how the performance of different microbiome analysis platforms impacts the final results of mucosal microbiome signatures.

Evaluation of AUTION EYE AI-4510 flow cell morphology analyzer for counting particles in urine.

Objectives: We evaluated the performance of a novel flow cell morphology analyzer AUTION EYE AI-4510 for counting particles in urine.

Methods: Analytical performance was assessed according to the EFLM European Urinalysis Guideline 2023. Trueness was compared by analyzing 1.

Diagnostic value of 11C-Methionine PET-CT imaging in persistent or recurrent Cushing's disease after surgery.

Introduction: Equivocal or negative pituitary magnetic resonance imaging (MRI) findings raise a significant challenge in the management of persistent or recurrent Cushing's disease (CD), compromising the chances of success of a further transsphenoidal surgery (TSS). The aim of our study was to determine the diagnostic utility of 11C-methionine (MET) positron emission tomography coupled with computerized tomography (PET/CT) in localizing the residual or relapsing corticotroph adenoma.

Methods: We retrospectively analyzed the results of 11C-MET PET/CT performed in two tertiary medical centers between May 2002 and November 2023 in 22 patients with a persisting or recurrent CD after initial TSS and equivocal or negative pituitary MRI findings.

Measurement of multidifferential cross sections for dijet production in proton-proton collisions at .

A measurement of the dijet production cross section is reported based on proton-proton collision data collected in 2016 at by the CMS experiment at the CERN LHC, corresponding to an integrated luminosity of up to 36.3 . Jets are reconstructed with the anti- algorithm for distance parameters of and 0.

Confocal Endomicroscopy Intestinal Epithelial Barrier Abnormalities in Individuals Without Documented Gastro-Intestinal Disease.

Background And Aims: Probe-based confocal endomicroscopy (pCLE) allows real-time microscopic visualization of the intestinal mucosa surface layers. Despite remission achieved through anti-tumor necrosis factor or vedolizumab therapy, anomalies in the intestinal epithelial barrier are observed in inflammatory bowel disease (IBD) patients. Our study aimed to assess these abnormalities in non-IBD individuals and compare them with IBD patients in endoscopic remission to identify the associated factors.

Simulation of adaptive immune receptors and repertoires with complex immune information to guide the development and benchmarking of AIRR machine learning.

Machine learning (ML) has shown great potential in the adaptive immune receptor repertoire (AIRR) field. However, there is a lack of large-scale ground-truth experimental AIRR data suitable for AIRR-ML-based disease diagnostics and therapeutics discovery. Simulated ground-truth AIRR data are required to complement the development and benchmarking of robust and interpretable AIRR-ML methods where experimental data is currently inaccessible or insufficient.

Efficacy and Safety of Mechanical Thrombectomy in Distal Medium Middle Cerebral Artery Occlusion Ischemic Stroke Patients on Low-Dose Aspirin.

Background: Acute ischemic stroke (AIS) from distal medium vessel occlusion (DMVO) presents unique treatment challenges. Mechanical thrombectomy (MT) is emerging as a viable option for these patients, yet the role of pre-stroke aspirin treatment is unclear. This study evaluates the impact of pre-stroke low-dose aspirin on outcomes in DMVO patients undergoing MT.

A novel artificial intelligence-powered tool for automated root canal segmentation in single-rooted teeth on cone-beam computed tomography.

Aim: To develop and validate an artificial intelligence (AI)-powered tool based on convolutional neural network (CNN) for automatic segmentation of root canals in single-rooted teeth using cone-beam computed tomography (CBCT).

Methodology: A total of 69 CBCT scans were retrospectively recruited from a hospital database and acquired from two devices with varying protocols. These scans were randomly assigned to the training (n = 31, 88 teeth), validation (n = 8, 15 teeth) and testing (n = 30, 120 teeth) sets.

Steam sterilisation of disposable surgical gowns does not compromise resistance to wet microbial penetration.

Background: Reuse of disposable medical products is common practice in veterinary medicine; however, studies on the safety of such reuse are often lacking. This study aimed to determine whether steam sterilisation of disposable surgical gowns compromises their resistance to wet microbial penetration.

Methods: Twenty surgical gowns were removed from their sterile packaging, manipulated, wetted, dried and subsequently repacked and steam sterilised.

Mammary Gland Reactive Stroma Characterization at Aging After Bisphenol A Exposure During Hormonal Susceptibility Windows.

Mammary glands development is influenced by endocrine signaling, which remodels epithelial and stromal compartments. Reactive stroma phenotype is observed when stromal disturbances occur, leading to changes in extracellular matrix composition and occurrence of reactive cell types. One of the triggers of these alterations is endocrine-disrupting chemical exposure, such as bisphenol A (BPA).

Evaluation of phenotypic risk indicators for the development of alopecia X (hair cycle arrest) in Pomeranian dogs in the Netherlands and Belgium.

Background: Alopecia X (AX) is a common noninflammatory alopecic condition of Pomeranian dogs with an unknown cause. While a genetic aetiology is suspected, no current tests can predict the development of this disorder or confirm the aetiology. Therefore, identifying potential risk indicators for the development of AX would be of value in this breed.

Enhancement of titanium surfaces using different acid solutions at room temperature to improve bone cell responses.

Background/purpose: Early osseointegration of titanium (Ti) dental implants relies on the surface topography. Surface modification of Ti seeks to enhance bone regeneration around implants. Acid etching is the simple, less technique sensitive and cost-effective technique for surface treatment.

Magnetic Resonance imaging evaluation of the distance to the popliteal artery in arthroscopic popliteus tendon versus posterior cruciate ligament reconstruction.

Purpose: Wide acceptance of arthroscopically treating posterolateral corner injuries has not occurred. There remains a fear of neurovascular (NV) injury while arthroscopically performing these reconstructions. The study's aim is to compare on Magnetic Resonance Scans the distance of the tibial tunnel in an arthroscopic popliteus tendon reconstruction (APB) and arthroscopic posterior cruciate ligament (PCL) reconstruction (APC) to the popliteal neurosvascular bundle.

Nebulized aminoglycosides for ventilator-associated pneumonia: Methodological considerations and lessons from experimental studies.

Aminoglycosides are concentration-dependent antibiotics exerting a bactericidal effect when concentrations at the site of infection are equal to or greater than 5 times the minimum inhibitory concentrations (MIC). When administered intravenously, they exhibit poor lung penetration and high systemic renal and ototoxicity, imposing to restrict their administration to 5 days. Experimental studies conducted in anesthetized and mechanically ventilated sheep and pigs provide evidence that high doses of nebulized aminoglycosides induce a rapid and potent bacterial killing in the infected lung parenchyma.

The crucial role of 18F-fluorodeoxyglucose positron emission tomography/computed tomography in diagnosing pulmonary valve endocarditis in patients after transcatheter pulmonary valve implantation: a case report.

Background: Patients after transcatheter pulmonary valve implantation (TPVI) are at increased risk for infective prosthetic valve endocarditis. Diagnosis of infective endocarditis (IE) following TPVI is particularly difficult due to impaired visualization of the transcatheter pulmonary valve (TPV) with echocardiography [Delgado V, Ajmone Marsan N, de Waha S, Bonaros N, Brida M, Burri H, et al. 2023 ESC guidelines for the management of endocarditis.

Application of the market-ready NAVETTA electrodeposition chamber for controlled exposure with nano-scaled aerosols.

Exposure of lung epithelia to aerosols is omnipresent. Chronic exposure to polluted air is a significant factor in the development of pulmonary diseases, which are among the top global causes of death, including COVID-19, chronic obstructive pulmonary disease, lung cancer, and tuberculosis. As efforts to prevent and treat lung diseases increase, the development of pulmonary drug delivery systems has become a major area of interest.

Treatment effects of CFTR modulators on people with cystic fibrosis carrying the Q359K/T360K variant.

https://bit.ly/45DjFw9.

Embedding-based pair generation for contrastive representation learning in audio-visual surveillance data.

Smart cities deploy various sensors such as microphones and RGB cameras to collect data to improve the safety and comfort of the citizens. As data annotation is expensive, self-supervised methods such as contrastive learning are used to learn audio-visual representations for downstream tasks. Focusing on surveillance data, we investigate two common limitations of audio-visual contrastive learning: false negatives and the minimal sufficient information bottleneck.

CytoNorm 2.0: A flexible normalization framework for cytometry data without requiring dedicated controls.

Cytometry is a single cell, high-dimensional, high-throughput technique that is being applied across a range of disciplines. However, many elements alongside the data acquisition process might give rise to technical variation in the dataset, called batch effects. CytoNorm is a normalization algorithm for batch effect removal in cytometry data that was originally published in 2020 and has been applied on a variety of datasets since then.

Aortic valve repair in adults: long-term clinical outcomes and echocardiographic evolution in different valve repair techniques.

Objective: Aortic valve repair/sparing have been established as effective treatments for aortic regurgitation and/or aortic aneurysms. However, concerns remain regarding long-term durability, reproducibility, and patient selection. This study aims to asses long-term clinical and echocardiographic outcomes, with a focus on aortic regurgitation grade and left ventricular ejection fraction evolution, in adults undergoing these procedures.

Multiple Sclerosis Nursing to Improve Care and Education (MSNICE): an observational study.

Objective: To explore differences in patient reported outcomes, health care resources and expenditures in persons with multiple sclerosis (pwMS) with or without access to an MS-nurse.

Methodology: An observational, multicenter and cross-sectional study was conducted. Seven centers with, and twelve centers without an MS-nurse participated.

The multi-omics signatures of telomere length in childhood.

Background: Telomere length is an important indicator of biological age and a complex multi-factor trait. To date, the telomere interactome for comprehending the high-dimensional biological aspects linked to telomere regulation during childhood remains unexplored. Here we describe the multi-omics signatures associated with childhood telomere length.

Developing a clinical prediction model to modify empirical antibiotics for non-typhoidal Salmonella bloodstream infection in children under-five in the Democratic Republic of Congo.

Background: Non-typhoidal Salmonella (NTS) frequently cause bloodstream infection in children under-five in sub-Saharan Africa, particularly in malaria-endemic areas. Due to increasing drug resistance, NTS are often not covered by standard-of-care empirical antibiotics for severe febrile illness. We developed a clinical prediction model to orient the choice of empirical antibiotics (standard-of-care versus alternative antibiotics) for children admitted to hospital in settings with high proportions of drug-resistant NTS.