Insect metamorphosis and chitin metabolism under miRNA regulation: a review with current advances.

Insect metamorphosis is a complex developmental process regulated by microRNAs (miRNAs) and hormonal signaling pathways. Key genes driving insect ontogenic changes are precisely modulated by miRNAs, which interact with 20-hydroxyecdysone (20E) and juvenile hormone (JH) to coordinate developmental transitions. Over the past decade, significant progress has been made in understanding miRNA biogenesis, their regulatory roles in gene expression, and their involvement in critical biological processes, including metamorphosis and chitin metabolism.

Genome-Wide Association Studies Reveal the Genetic Architecture of Ionomic Variation in Grains of Tartary Buckwheat.

Tartary buckwheat (Fagopyrum tataricum) is esteemed as a medicinal crop due to its high nutritional and health value. However, the genetic basis for the variations in Tartary buckwheat grain ionome remains inadequately understood. Through genome-wide association studies (GWAS) on grain ionome, 52 genetic loci are identified associated with 10 elements undergoing selection.

Recommendations for Design, Execution, and Reporting of Studies on Experimental Thoracic Aortopathy in Preclinical Models.

There is a recent dramatic increase in research on thoracic aortic diseases that includes aneurysms, dissections, and rupture. Experimental studies predominantly use mice in which aortopathy is induced by chemical interventions, genetic manipulations, or both. Many parameters should be deliberated in experimental design in concert with multiple considerations when providing dimensional data and characterization of aortic tissues.

Novel insights into human CRISP2: localization in reproductive tissues and sperm, and molecular characterization.

CRISP2 is enriched in the male reproductive system of mammals and plays roles in spermatogenesis, sperm motility, and fertilization. Although extensively investigated in rodents and boars, human CRISP2 (hCRISP2) remains poorly studied, particularly concerning its localization in testicular and epididymal tissues and its molecular features. In this study, we used immunofluorescence to determine the localization of hCRISP2 in testis, epididymis, and ejaculated sperm.

Real-time multispectral imaging for intraoperative monitoring of coronary artery bypass graft patency.

Significance: Coronary artery disease is the leading cause of death worldwide, accounting for 16% of all deaths. A common treatment is coronary artery bypass grafting (CABG), though up to 12% of bypass grafts fail during surgery. Early detection of graft failure by intraoperative graft patency assessment could prevent severe complications.

 gen. et sp. nov. (Mytilinidiaceae), and (Dactylosporaceae), a new lichenicolous species from Yunnan, China.

and are reported from China and identified through DNA sequence analyses (LSU, ITS, and 1-α) and morphological characteristics. Phylogenetic analysis showed that forms a distinct lineage within Mytilinidiaceae, closely related to the subclade, leading to the establishment of a new genus within this family. This saprotrophic species grows on the bark of , often surrounded by the thallus of species.

 and -two additional new species from the Neotropics (Peltigerales, Peltigeraceae).

Two additional species of are described as new to science based on material from Bolivia and Peru and supported by phylogenetic analysis of the fungal ITS barcoding marker. The two new species represent lineages within clade I on the global phylogeny. Ossowska, B.

Thrombotic Microangiopathy: A Devastating Complication After Lung Transplantation.

Background: Thrombotic microangiopathy (TMA) following lung transplantation (LTx) is a rare but severe complication. The pathogenesis is poorly understood, and various risk factors have been suggested. In this study, we aimed to evaluate diagnostic accuracy, identify risk factors, and assess renal, pulmonary, and overall survival of TMA in this patient group.

Belonging: a meta-theme analysis of women's community-making in group antenatal and postnatal care.

Health care systems are social institutions simulating microcosms of wider societies where unequal distribution of power and resources translate into inequities in health outcomes, experiences and access to services. Growing research on participatory women's groups positively highlights the influence of group-based care on health and wellbeing for women, their infants, families and wider communities across different countries. With similarities in ethos and philosophies, group care combines relational, group-based facilitation and clinical care, uniquely offering an opportunity to examine the intersections of health and social care.

Generational differences in healthcare: the role of technology in the path forward.

Healthcare systems worldwide are under increasing pressure due to aging populations, rising prevalence of chronic diseases, and heightened patient expectations. Generational differences significantly impact perceptions of health, healthcare decision-making, use of digital technologies, and attitudes toward preventative health. This perspective article explores these differences through the lens of Generational Cohort Theory, focusing on six generations: the Silent Generation, Baby Boomers, Generation X, Millennials, Generation Z, and Generation Alpha.

Mapping the landscape of vitamin D in cancer studies: a systematic global investigation.

Purpose: This comprehensive study examines the multifaceted relationship between vitamin D and cancer, synthesizing key scientific advancements and global research trends to guide future investigations and address critical gaps in the field.

Methods: Publications on vitamin D and cancer were retrieved from Scopus up to November 2024. English-language original and review articles were analyzed using Excel, VOSviewer, and Scimago Graphica, focusing on publication trends, citation impacts, and research themes.

Cysteine-selective [Re]Re(v) radiolabelling of a Nanobody® for targeted radionuclide therapy using a "chelate-then-click" approach.

In this study, we present the first reported use of bioorthogonal click chemistry with rhenium-188 for radiolabelling of an anti-c-Met VH Nanobody®. We employed a "chelate-then-click" strategy, wherein a bifunctional chelator was designed in two parts, which were subsequently joined post-labelling and post-conjugation the strain-promoted azide-alkyne cycloaddition (SPAAC) reaction. Cysteine-selective conjugation of the VH was achieved through thiol-Michael addition, forming a VH-DBCO construct.

Detecting imported malaria infections in endemic settings using molecular surveillance: current state and challenges.

The Global Technical Strategy for Malaria 2016-2030 targets eliminating malaria from at least 35 countries and reducing case incidence by 90% globally. The importation of parasites due to human mobilization poses a significant obstacle to achieve malaria elimination as it can undermine the effectiveness of local interventions. Gaining a comprehensive understanding of parasite importation is essential to support control efforts and advance progress toward elimination.

Out of Breath, out of Options: Benralizumab as a Last Hope in ICU-Treated Near-Fatal Eosinophilic Asthma: A Case Series.

Asthma exacerbations remain life-threatening events despite advancements in biologic therapies. This case series reports on four patients with near-fatal eosinophilic asthma exacerbations who had been admitted to intensive care and were treated with benralizumab as a last resort after failing maximal standard therapies. All patients exhibited marked blood or airway eosinophilia and required intensive care ventilatory support.

Morphosyntactic production and processing skills in relation to age effects and lexical-phonological levels among children with cochlear implants and typically hearing peers: a focus on vowel nasality.

Introduction: Significant variability in the language performance of children with cochlear implant (CI) is widely recognized in the literature, particularly concerning morphosyntactic (MS) skills. The perceptual limitations of the CI, which can lead to phonological difficulties, may be responsible for this increased vulnerability in grammatical abilities. In this context, the present study focuses on the morphophonemic processing of items distinguished by nasal and oral vowels in the French language - the feature of vowel nasality being known as challenging for the CI population.

Investigation of the role of miRNA variants in neurodegenerative brain diseases.

Introduction: miRNAs are small noncoding elements known to regulate different molecular processes, including developmental and executive functions in the brain. Dysregulation of miRNAs could contribute to brain neurodegeneration, as suggested by miRNA profiling studies of individuals suffering from neurodegenerative brain diseases (NBDs). Here, we report rare miRNA variants in patients with Alzheimer's dementia (AD) and frontotemporal dementia (FTD).

Human oocyte capacitation culture: Essential step toward hormone-free assisted reproductive technology.

Background: In vitro oocyte maturation (IVM) is not a novel concept; however, its wide-scale practice has been limited because of the lower clinical outcomes compared to conventional assisted reproductive technologies.

Methods: This comprehensive review addresses the significant advances made in oocyte in vitro maturation with the biphasic capacitation (CAPA)-IVM strategy applied to small ovarian antral follicles in humans over the last 10 years. CAPA-IVM consists of a prematuration phase wherein immature oocytes are temporarily meiotically arrested to gain competence before undergoing meiotic resumption.

Myositis of pterygoid muscles and superior ophthalmic vein and cavernous sinus thrombosis in active Crohn's disease undergoing ustekinumab treatment: a case report and literature review.

Introduction: Myositis is a rare extra-intestinal presentation in patients with inflammatory bowel diseases (IBD), and its occurrence has only been described in a few case reports. However, it is essential to consider other potential causes as patients with IBD are more susceptible to infections due to their immunocompromised status, which may also be exacerbated by immunosuppressive drugs contributing to myositis. Our case highlights the complexity and challenges in diagnosing and managing myositis in patients with IBD as well as a review of the literature.

Nickel-Catalyzed Stereospecific Alkylation of Vinylsiloxanes Using Pyridinium Salts.

A methodology for radical cross-coupling with vinylsiloxanes and pyridinium salts under nickel catalysis is described. Easily implemented from inexpensive and abundant primary amines and terminal alkynes, this Hiyama coupling provides efficient access to (), (), and ()-alkenes with selectivity control. Operating under mild conditions, this robust strategy applies to a broad range of functional groups with diverse double bond stereochemistries.

Diagnostics, reconstructive approaches, and outcomes in female urethroplasty patients.

Objectives: To gain further understanding of current outcomes regarding urethroplasty for female urethral stricture disease.

Methods: The European Association of Urology Section of Genitourinary Reconstructive Surgeons formed the Female Reconstruction Group to enhance multicentric collaboration, and to generate more evidence on the most effective treatment options for female patients with recurrent urethral stricture. Patients were eligible for inclusion if they had a urethral stricture and had undergone one of three reconstructive procedures, namely ventral onlay flap (VOFU), ventral onlay graft (VOGU) or dorsal onlay graft (DOGU) urethroplasty.

Demonstration of virtual imaging trial applications for optimization and education of dento-maxillofacial CBCT imaging.

Background: A number of studies have suggested that there is a need for improved understanding of dento-maxillofacial cone beam computed tomography (CBCT) technology, and to establish optimized imaging protocols. While several ex vivo/in vitro studies, along with a few in vivo studies, have addressed this topic, virtual imaging trials could form a powerful alternative but have not yet been introduced within the field of dento-maxillofacial imaging.

Purpose: To introduce and illustrate the potential of utilizing a virtual imaging trial (VIT) platform for dento-maxillofacial CBCT imaging through a number of case studies.

A Comprehensive Review of Instrumentation and Applications in Post-Column and In-Source Derivatization for LC-MS.

Liquid chromatography-mass spectrometry (LC-MS) has become an indispensable tool for elucidating molecular structures and quantifying diverse compounds within complex mixtures. Despite its versatility, it faces various challenges such as ion suppression, low sensitivity, analyte instability, and matrix effects, which are being overcome by different kinds of offline and online derivatization techniques to improve specificity and reduce potential interferences. In this context, considerable advancements have been made in reviewing and critically evaluating a wide range of developed methods and techniques; however, little attention has been given to post-column derivatization (PCD) in LC-MS.

Research Review: Help-seeking intentions, behaviors, and barriers in college students - a systematic review and meta-analysis.

Background: The prevalence of mental health problems among college students has increased over the past decade. Even when mental health services are available, many students still struggle to access these services. This systematic review and meta-analysis aimed to identify the rates at which students actively seek or consider using formal help and to determine the main reasons for not seeking help.

The Effects of Combined Scutellaria and Saffron Supplementation on Mood Regulation in Participants with Mild-to-Moderate Depressive Symptoms: A Randomized, Double-Blind, Placebo-Controlled Study.

The global prevalence of mental health disorders, particularly anxiety and depression, has increased significantly, with rates further elevated by the COVID-19 pandemic. Conventional pharmacological treatments, while effective, often lead to side effects that can impact patient adherence and quality of life. This has driven interest in safer, natural adjunctive therapies.

Nanoarchitectonics of Sustainable Food Packaging: Materials, Methods, and Environmental Factors.

Nanoarchitectonics influences the properties of objects at micro- and even macro-scales, aiming to develop better structures for protection of product. Although its applications were analyzed in different areas, nanoarchitectonics of food packaging-the focus of this review-has not been discussed, to the best of our knowledge. The (A) structural and (B) functional hierarchy of food packaging is discussed here for the enhancement of protection, extending shelf-life, and preserving the nutritional quality of diverse products including meat, fish, dairy, fruits, vegetables, gelled items, and beverages.