Altering Carotene Hydroxylase Activity of DcCYP97C1 Affects Carotenoid Flux and Changes Taproot Colour in Carrot.

CYP97C1 as a haem-containing cytochrome P450 hydroxylase (P450-type) is important for carotene hydroxylation and xanthophyll biosynthesis. Research about this type of hydroxylase was mainly reported in several model plant species which have no specialized tissues accumulating massive carotenoids. The function of CYP97C1 in the horticultural plant, like carrots, was not fully studied.

Genome-Wide Identification and Expression Analysis of /s in L.

Pepper ( L.) is a popular vegetable in people's daily lives. During pepper growth, calcium (Ca) is an essential macronutrient, and calcium-transporting ATPase (ACA/ECA) is a vital protein for calcium transport.

Evolution of a SHOOTMERISTEMLESS transcription factor binding site promotes fruit shape determination.

In animals and plants, organ shape is primarily determined during primordium development by carefully coordinated growth and cell division. Rare examples of post-primordial change in morphology (reshaping) exist that offer tractable systems for the study of mechanisms required for organ shape determination and diversification. One such example is morphogenesis in Capsella fruits whose heart-shaped appearance emerges by reshaping of the ovate spheroid gynoecium upon fertilization.

Telomere-to-telomere genome assembly of eggplant (Solanum melongena L.) promotes gene fine localization of the green stripe (GS) in pericarp.

Fruit appearance of eggplant is a key commercial trait, and the precise selection of new varieties with diverse aesthetics aligns with current breeding objectives. However, functional genomics research in eggplant remains underdeveloped. Here, we assembled the first telomere-to-telomere (T2T) eggplant genome, as well as chloroplast and mitochondrial genomes for the inbred line 'NO211'.

A natural variant of NON-RIPENING promotes fruit ripening in watermelon.

The regulation of non-climacteric fruit ripening by the transcription factor NON-RIPENING (NOR) is poorly understood. Here, we identified that the NOR homolog in the non-climacteric fruit watermelon (Citrullus lanatus) was located within the selective sweep and sweetness quantitative trait locus that was selected during domestication from landraces to cultivars. ClNOR knockout substantially delayed fruit ripening, and the fruits of the knockout plants had lower abscisic acid (ABA) levels, lighter colored flesh, and were less sweet compared to wild type.

Effects of blanching cultivation on the chemical composition and nutritional quality of Chinese chive.

Blanching is an agricultural practice where vegetables are cultivated in darkness to prevent photosynthesis, thereby modifying their colour, texture, and flavor. The technique is popularly employed in Chinese chive (CC). Blanched Chinese chive (BCC) is renowned for its pale-yellow appearance, delicate flavor, and culinary-medicinal values; nonetheless, how blanching alters the chemical composition largely remains intangible.

Editing in the Watermelon Genome Using CRISPR/Cas9 Technology Confers Resistance to ZYMV.

Watermelon is one of the most important cucurbit crops, but its production is seriously affected by viral infections. Although eIF4E proteins have emerged as the major mediators of the resistance to viral infections, the mechanism underlying the contributions of eIF4E to watermelon disease resistance remains unclear. In this study, three genes and one gene were identified in the watermelon genome.

The dynamic N-methyladenosine RNA methylation provides insights into the tomato fruit ripening.

N-methyladenosine (mA) methylation is an essential mechanism of gene regulation known to impact several biological processes in living organisms. However, little is known about the abundance, distribution, and functional significance of mRNA mA modification during fruit ripening of tomato the main model species for fleshy fruits. Our study shows that mA modifications are prevalent in tomato mRNA and are detected in lncRNA and circRNA.

Identification of Powdery Mildew Resistance-Related Genes in Butternut Squash ().

Powdery mildew infection is a significant challenge in butternut squash () production during winter in Hainan, China. The tropical climate of Hainan promotes powdery mildew infection, resulting in substantial yield losses. By utilizing transcriptome and genome sequencing data, SNPs and potential genes associated with powdery mildew resistance in butternut squash were identified.

Recurrent excision of a hAT-like transposable element in CmAPRR2 leads to the "shooting star" melon phenotype.

The external appearance of fruit commodities is an essential trait that has profound effects on consumer preferences. A natural melon variety, characterized by an uneven and patchy arrangement of dark green streaks and spots on the white-skinned rind, resembles shooting stars streaking across the sky; thus, this variety is called "Shooting Star" (SS). To investigate the mechanism underlying the SS melon rind pattern, we initially discovered that the variegated dark green color results from chlorophyll accumulation on the white skin.

ACL1-ROC4/5 complex reveals a common mechanism in rice response to brown planthopper infestation and drought.

Brown planthopper (BPH) is the most destructive insect pest of rice. Drought is the most detrimental environmental stress. BPH infestation causes adaxial leaf-rolling and bulliform cells (BCs) shrinkage similar to drought.

A near-complete chromosome-level genome assembly of looseleaf lettuce (Lactuca sativa var. crispa).

Lettuce (Lactuca sativa L., Asteraceae) is one of the most important vegetable crops, known for its various horticultural types and significant morphological variation. The first reference genome of lettuce, a crisphead type (L.

eIF2Bβ confers resistance to Turnip mosaic virus by recruiting ALKBH9B to modify viral RNA methylation.

Eukaryotic translation initiation factors (eIFs) are the primary targets for overcoming RNA virus resistance in plants. In a previous study, we mapped a BjeIF2Bβ from Brassica juncea representing a new class of plant virus resistance genes associated with resistance to Turnip mosaic virus (TuMV). However, the mechanism underlying eIF2Bβ-mediated virus resistance remains unclear.

The pleiotropic functions of GOLDEN2-LIKE transcription factors in plants.

The regulation of gene expression is crucial for biological plant growth and development, with transcription factors (TFs) serving as key switches in this regulatory mechanism. GOLDEN2-LIKE (GLK) TFs are a class of functionally partially redundant nuclear TFs belonging to the GARP superfamily of MYB TFs that play a key role in regulating genes related to photosynthesis and chloroplast biogenesis. Here, we summarized the current knowledge of the pleiotropic roles of GLKs in plants.

Effect of light on ascorbic acid biosynthesis and bioinformatics analysis of related genes in Chinese chives.

Ascorbic acid (AsA) is an essential nutritional component and powerful antioxidant in vegetables, and in plants, AsA levels are regulated by light. AsA levels in the leaves of Chinese chive (Allium tuberosum Rottler ex Spr), a popular vegetable, are poorly understood. Thus, this study was performed to assess the influence of light on AsA biosynthesis in chive and select related genes (AtuGGP1 and AtuGME1); in addition, bioinformatic analyses and gene expression level assays were performed.

A comprehensive physiological and -Omic analysis of trypsin-mediated protection of green pepper fruits from chilling injury.

Chilling injury (CI) in green pepper fruits during low-temperature storage causes a significant decline in quality. The present study utilized physiological, transcriptomic, and metabolomic analyses to idneitfy the mechanisms by which trypsin mitigates CI in green peppers stored at 4 °C for 8 days, followed by 3 days of shelf life. Results indicated that the trypsin treatment significantly reduced electrolyte leakage and the CI index in peppers, effectively extending their shelf life and preserving postharvest quality.

AcMYB96 promotes anthocyanin accumulation in onion (Allium cepa L) without forming the MBW complex.

Anthocyanins are major flavonoid compounds with established health benefits. Although the molecular mechanisms of MYB transcription factors (TFs) within the MYB-basic helix-loop-helix (bHLH)-WD-repeat protein (MBW) complex in anthocyanin biosynthesis have been revealed, the functions of other MYB TFs that are unable to form the MBW complex in this process remain unclear. In this study, we uncovered and extensively characterized an R2R3-MYB TF in onion (Allium cepa L.

Moderately Elevated Temperature Offsets the Adverse Effects of Waterlogging Stress on Tomato.

Global warming and waterlogging stress due to climate change are expected to continue influencing agricultural production worldwide. In the field, two or more environmental stresses usually happen simultaneously, inducing more complex responses in plants compared with individual stresses. Our aim was to clarify how the two key factors (temperature and water) interacted and influenced physiological response and plant growth in tomatoes under ambient temperature, moderately elevated temperature, waterlogging stress, and moderately elevated temperature and waterlogging stress.

SlWRKY37 targets SlLEA2 and SlABI5-like7 to regulate seed germination vigor in tomato.

Seed germination is a critical phase for the life cycle and propagation of higher plants. This study explores the role of SlWRKY37, a WRKY transcription factor in tomato, in modulating seed germination. We discovered that SlWRKY37 expression is markedly downregulated during tomato seed germination.

Ectoine maintains the flavor and nutritional quality of broccoli during postharvest storage.

The bacterial derived osmolyte ectoine has been shown to stabilize cell structure and function, a property that may help to extend the shelf life of broccoli. The impact of ectoine on broccoli stored for 4 d at 20 °C and 90% relative humidity was investigated. Results indicated that 0.