[Advances of clinical studies of acupuncture and moxibustion for treatment of rheumatoid arthritis].

Objective: To summarize recent development of studies on acupuncture and moxibustion for prevention and treatment of rheumatoid arthritis, so as to provide a basis and thinking for clinical scientific studies.

Methods: More than 60 papers about clinical and experimental studies were reviewed, choosing clinically commonly-used acupoints and introducing mainly different therapeutic methods.

Results: Acupuncture and moxibustion can prevent and cure rheumatoid arthritis with outstanding results.

[On acupuncture and moxibustion treatment based on the layers of "skin, vessel, muscle, tendon and bone"].

Objective: To expound the therapeutic method based on the layers of "skin, vessel, muscle, tendon and bone" and its clinical values.

Methods: Systematically explore the literature relative to treatments based on the layers in The Yellow Emperor's Internal Classic, cite, analyze and summarize contents of nine types of needling and needling methods, etc., relative to treatments based on the layers.

[Effect of drugs for promoting blood circulation on blood lipids and inflammatory reaction of atherosclerotic plaques in ApoE gene deficiency mice].

Objective: To observe the effect of six common Chinese medicinal herbs for promoting blood circulation, including Radix Paeoniae rubra (I), Radix Salviae miltiorrhizae (II), Rhizoma Chuanxiong (III), Radix Notoginseng (IV), Semen Persicae (V) and wine steamed Radix et Rhizoma Rhei (VI), on blood lipids and inflammatory reaction of atherosclerotic plaques in ApoE gene deficiency mice.

Methods: Ninety mice, 6 - 8 weeks old, were divided into 8 groups, the model group, the control group (treated with simvastatin) and the six treated groups treated with the above-mentioned 6 Chinese medicinal herbs respectively. All the mice were fed with the diet of western kind for 13 weeks until the mature atherosclerotic plaques formed in them.

[Effect of danshensu and ligustrazine on related genes of myocardial hypertrophy induced by angiotensin II].

Objective: To study the effect of Danshensu (DSS) and Ligustrazine (TMZ), the extracts of Chinese herbs for promoting blood circulation, on angiotensin II (Ang II) induced myocardial hypertrophy and its related genes, and to explore the mechanisms of inhibitory effect.

Methods: Adopting one-step method, the total RNA of myocardial cells was extracted by TRIzol reagent. Then the expression of ANP and beta-actin mRNA, as symbol of myocardial cells, were detected by RT-PCR.

[Influence of bondage stress on Th1/Th2 cytokines and growth of tumors in mice bearing S180 tumor cells].

Aim: To observe the influence of bondage stress on Th1/Th2 cytokines and growth of tumor in mice bearing S180 tumor cells.

Methods: 2 x 10(6) S180 tumor cells were injected subcutaneously into the murine right oxter,and bondage stress was imposed on mice for 8 hours per day,ten days running. Then T lymphocyte proliferation was detected by MTT colorimetry.

[Determination of adulteration musk by GC-MS, SPME and HS2000].

Musk is one of the rare traditional Chinese medicinal materials and is usually sophisticated with different adulterants. Some new adulterants have been detected by GC-MS, SPME and HS2000.

[Investigation on circulation of Ao shi shang han jin jing lu (Ao's Golden mirror of cold pathogenic diseases) in Japan].

Being the earliest extant work on tongue diagnostics, Ao shi jin jing lu was block-printed and published in 1654 after it was spread into Japan, on the basis of which many kinds of manuscripts were made. This book not only exerted profound influence on the Kampo diagnostics in Edo age, but also laid down a foundation for the formation of pulse diagnostic school of tongue diagnostics in Kampo medicine.

[Experimental study on cardiac protection and anti-oxidation effects of jisheng injection].

Objective: To observe the effect of Jisheng injection (JSI) in protecting heart.

Methods: Isolated heart of rat was preserved in modified Euro-Collins solution (mEC) containing JSI for 20 hrs, and that preserved in simple mEC was taken as control. Then Langendorff isolated rat heart perfusion was conducted.

[Study on quality standard for heshouwu dispensing granule].

Objective: To study the quality standard for Heshouwu dispensing granule.

Methods: Heshouwu dispensing granule was identified by TLC and 2,3,5,4'-tetrahydroxy-stilbene-2-O-beta-D-glucoside in Heshouwu dispensing granule were determined by HPLC.

Results: The linear relationship was at the range of 0.

[Study on the genetiey relationship between Chinese and Korean medicinal materials of niuxi with the method of RAPD].

Objective: To study the hereditary relationship between Chinese and Korean medicinal materials of Niuxi achyranthis root.

Method: Ten samples of four kinds of Niuxi was studied with the method of RAPD.

Result: Expanded product showed total record of 100 spectrum bands, which proved that the hereditary gap between Korean self-produced A.

[Comparative study on effect of recipe for activating blood circulation and replenishing Qi on left ventricular remodeling in rats with left heart failure after myocardial infarction].

Objective: Comparative study to the effect of Chinese herbal medicine on left ventricular remodeling in rats with left heart failure after myocardial infarction (MI).

Methods: Rat's model of left heart failure after myocardial infarction was treated with injection for activating blood circulation (ABCI, consisted of R. Salviae miltiorrhizea; Rh.

[Experimental study on protective effective of congsheng capsule in acute cerebral ischemia and improvement of cerebral blood flow and energy metabolism].

Objective: To investigate the protective effect of Congsheng capsule (CSC) on acute cerebral ischemia in mice and rats.

Methods: Cerebral ischemia model was established by adding FeCl3 to block the blood flow of unilateral middle cerebral artery (MCA) for 30 mins. Acute incomplete cerebral ischemia was induced by ligation of bilateral carotid arteries in rats.

[Effect of xiaoyin jiedu yin on Staphylococcus aureus enterotoxin B induced serum interleukin 8 in mice].

Objective: To explore the therapeutic mechanism of Xiaoyin Jiedu Yin (XYJDY) in treating psoriasis.

Methods: Abnormal elevation of interleukin 8 (IL-8) level in mice was induced by Staphylococcus aureus enterotoxin B (SEB), then the antagonizing effects of XYJDY and Composite Qingdai Capsule were observed.

Results: Serum IL-8 level arose and reached the peak 3-5 hrs after SEB induction.

[Experimental study on effect of bushen huoxue xiezhuo decoction in treating minimal change nephropathy].

Objective: To explore the therapeutic effect and mechanism of Bushen Huoxue Xiezhuo Decoction (BHXD) in treating minimal change nephropathy (MCN) in rats.

Methods: The MCN model rats established by a single intravenous injection of Adriamycin were divided into the model, BHXD group, and a normal group was set up for control. The effect of treatment on renal function, hemorrheologic parameters, renal tissue TGF-beta 1 expression and polyanion sites on glomerular basement membrane were observed dynamically.

[Experimental study on effect of congsheng capsule on expression of brain derived neurotrophic factor and tyrosinkinase mRNA in forebrain of rats after cortical devascularization].

Objective: To study the effect and mechanism of Congsheng Capsule (CSC) on brain derived neurotrophic factor (BDNF) and tyrosinkinase (trkB) mRNA in forebrain of rats after cortical devascularization.

Methods: The rat devascularization model was established, and expression of BDNF mRNA and trkB mRNA were determined by hybridization in situ method.

Results: Expression of BDNF mRNA and trkB mRNA reduced obviously in the cerebral cortex and hippocampus of devascularization model, especially in hippocampal CA1 subregion.

[Effect of conventionally prepared rabbit serum on human umbilical vein endothelial cell-304].

Objective: To study the effect of conventionally prepared rabbit serum, usually used in the field of serum pharmacology of TCM, in various concentration on cultured system of human umbilical vein endothelial cell (VEC)-304 cell line.

Methods: Prepared conventionally from healthy rabbit, the effect of uncontaminated and nonhemolytic serum of six concentrations (5%, 10%, 20%, 40%, 60% and 80%) on VEC-304 cells was observed using parameters such as cell morphologic examination, trypan blue exclusion test, methyl thiazolyl tetrazolium (MTT) test, lactate dehydrogenase (LDH) and angiotensin-converting release test.

Results: Serum of 5% and 10% concentration showed no effect in all the above-mentioned tests.

[Determination of alkaloids in Coptis-Cinnamomum compatibility by capillary electrophoresis].

Objective: To establish a HPCE method to determine the contents of alkalods in Rhizoma Coptidis.

Method: HPCE.

Result: The constituents thus determined have good linearity and separation.

[Effect of fusheng powder on neuropeptides in brains of rats with hyperlipidemia after cerebral ischemia and reperfusion].

Objective: To observe the changes of some neuropeptides and the effect of Fusheng powder (FSP) on neuropeptides in rat's brains in a stable cerebral ischemia and reperfusion (I/L) model.

Methods: The models of rat's brain injured were established by repeated cerebral I/R in rats with hyperlipidemia. Radioimmunoassay (RIA) was performed to determine the level of neuropeptides.

[Clinical study on retarding aging effect of tongbu recipe to traditional Chinese medicine].

Objective: To study the mechanism of Tongbu No. 1 (TB1, a prescription for reinforcing Kidney and Spleen, clearing up the bowel viscera to send Turbid downward and regulating Qi and blood) in retarding aging.

Methods: A controlled, multiple indexes study was conducted in 56 old subjects randomized into 3 groups.

[Effect of caoguo zhimu decoction on N-methyl-D-aspartate receptor gene expression of epileptic kindling model in rats].

Objective: To explore the effect of Caoguo Zhimu decoction (CGZMD) on N-methyl-D-aspartate (NMDA) receptor gene expression of epileptic model on rat brain.

Methods: Adopting the pentylenetetrazol (PTZ) kindling model to observe the NMDAR1 mRNA gene expression by situs hybridization.

Results: After 28 days of CGZMD treatment, the NMDAR1 mRNA gene expression on CA1, CA2, CA3 part of rat hippocampus reduced obviously, the comparison between experimental group and the model control group showed significant difference (P < 0.

[Wang Yinglin and his answer to the questions of a Korean physician].

The book named Da Chaoxianyi Wen (Answer to the Questions of a Korean Physician), written in 1624, recorded the written answers of Wang Yinglin, a Chinese official in the Ming dynasty, to the questions of Yin Zhiwei, a Korean official physician. The 24 A&Qs concerned about the therapy of ordinary diseases, channels and the theories of TCM, etc. The study of this book could not only clarify various questions on the exchanges between Wang and Yin, but also learn more information on the development of Korean medicine at that time.

[Evolution of conception on apoplexoid (lei zhong feng) disorder].

Viewing separately from the etiology and the disease itself, the ancient literature gave two definitions, totally different from each other, for apoplexoid disorders within the category of apoplexy, each experienced a process of starting, development and evolution stages. Both exerted an active promotion to the development of apoplexy.

[An outline on the early pharmacetical development before Galen].

The period before Galen (including his time) is known as ancient herbal period of pharmacy, which is an important component in the developing history of pharmacy. Some characteristics and rules about early pharmacy development are reflectd from the origin of pharmacy, Babylonian - Assyrian pharmacy, ancient Egyptian pharmacy, ancient Greek pharmacy and Rome pharmacy in different periods and areas.

[Study on the date of compilation of Wen bing tiao bian (Systemic Discourse on Warm Diseases)].

It has long been recognized that Wu Jutong compiled Wen bing tiao bian in the year 1798. Through study on the printing time of the book, prefaces in the book written by Zhu Bin and Zheng Bao, and the contents of the book, it is claimed that the book was written in 1798, and by then his work was not yet finished. The first manuscript was completed in 1804, finalized and printed in 1813.

[Effect of Zhongfeng Naodeping Granule on hippocampal excitatory amino acid and neuron of stroke-prone spontaneously hypertensive rats with hemorrhagic apoplexy].

Objective: To observe the effects of Zhongfeng Naodeping Granule (ZFNDPG) on hemorrhagic apoplexy.

Methods: The stroke-prone spontaneously hypertensive rats (SHRsp) were used to study effects of ZFNDPG on hemorrhage apoplexy. Excitatory amino acid (EAA) concentration in hippocampus sector, neuronal density and ultrastructural changes in hippocampal CA1 sector were measured.