An Effective and Efficient Sample Preparation Method for 2-Methyl-Isoborneol and Geosmin in Fish and Their Analysis by Gas Chromatography-Mass Spectrometry.

The intensive aquaculture strategy and recirculating aquaculture system often lead to the production of off-flavor compounds such as 2-methyl-isoborneol (2-MIB) and Geosmin (GSM). The regular purge and trap extraction followed by analysis with gas chromatography-mass spectrometry (GC-MS) usually involve a complicated assembly of facilities, more working space, long sample preparation time, and headspace solid-phase microextraction (SPME). In this work, a method with easier sample preparation, fewer and simplified facilities, and without SPME on GC-MS analysis is developed for the determination of 2-MIB and GSM in fish samples.

g-CN/FeO Nanocomposites as Adsorbents Analyzed by UPLC-MS/MS for Highly Sensitive Simultaneous Determination of 27 Mycotoxins in Maize: Aiming at Increasing Purification Efficiency and Reducing Time.

According to known studies, numerous mycotoxins have been found simultaneously in foods and have a certain expansion toxicity, so the simultaneous detection of multiple mycotoxins is absolutely critical. In this article, multifunctional magnetic g-CN/FeO nanocomposites have been fabricated to employ as modified QuEChERS adsorbents. In addition, they were also used in conjunction with ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), an accurate quantitative approach, to analyze 27 mycotoxins in maize.

A microfluidic colorimetric biosensor for in-field detection of Salmonella in fresh-cut vegetables using thiolated polystyrene microspheres, hose-based microvalve and smartphone imaging APP.

A microfluidic colorimetric biosensor was developed using thiolated polystyrene microspheres (SH-PSs) for aggregating of gold nanoparticles (AuNPs), a novel hose-based microvalve for controlling the flow direction, and a smartphone imaging APP for monitoring colorimetric signals. Aptamer-PS-cysteamine conjugates were used as detection probes and reacted with Salmonella in samples. Complementary DNA - magnetic nanoparticle (cDNA - MNP) conjugates were used as capture probes, reacted with the free aptamer-PS-cysteamine conjugates.

[Levels, Characteristics, and Potential Source of Micro(meso)plastic Pollution of Soil in Liaohe River Basin].

With the Liaohe River basin as the research object, the morphology, abundance, and distribution characteristics of micro(meso) plastics of soil in Liaohe River basin were studied based on the density flotation principle and the technique of stereo microscope and micro-FTIR and the significant factors affecting the distribution of the micro(meso)plastics and their potential sources were reasonably speculated. The results indicated that the average abundance of the soil micro(meso)plastic is (145.83±211.

Rapid transport of organochlorine pesticides (OCPs) in multimedia environment from karst area.

Karst groundwater is crucial, but particularly vulnerable to contaminants. Anthropologically derived pollutants on the surface-environment in karst areas could easily and rapidly enter groundwater through highly developed transmissible structures and threaten water safety. To investigate such transport, we analyzed 24 organochlorine pesticides (OCPs) in the multimedia environment from the Zigui karst area of China, where agriculture is the predominant human activity.

Comparative Study on the Protective Effect of Chlorogenic Acid and 3-(3-Hydroxyphenyl) Propionic Acid against Cadmium-Induced Erythrocyte Cytotoxicity: and Evaluation.

The metabolism of chlorogenic acid (CGA) through the intestinal tract was studied. As cadmium is a well-known toxic heavy metal, this study was carried out to investigate the comparative protective effect of CGA and its representative intestinal metabolite (3-(3-hydroxyphenyl) propionic acid, HPPA) against Cd-induced erythrocyte cytotoxicity and . We found that CGA and its intestinal metabolite appreciably prevented erythrocyte hemolysis, osmotic fragility, and oxidative stress induced by Cd.

DNAzyme Amplified Aptasensing Platform for Ochratoxin A Detection Using a Personal Glucose Meter.

Aptamer-based sensors have emerged as a major platform for detecting small-molecular targets, because aptamers can be selected to bind these small molecules with higher affinity and selectivity than other receptors such as antibodies. However, portable, accurate, sensitive, and affordable detection of these targets remains a challenge. In this work, we developed an aptasensing platform incorporating magnetic beads and a DNAzyme for signal amplification, resulting in high sensitivity.

Advances in gold nanoparticles for mycotoxin analysis.

Mycotoxins are toxic secondary metabolites naturally produced by fungi. They can cause various kinds of acute and chronic diseases in both humans and animals since food usually contains trace amounts of mycotoxins. Thus, it is important to develop a rapid and sensitive technique for mycotoxin detection.

Characterization of free, conjugated, and bound phenolics in early and late ripening kiwifruit cultivars.

Background: Kiwifruit (Actinidia) has long been called the 'king of fruits' because of its unique flavor and the wide range of bioactive compounds which contains ascorbic acid, phenolics and minerals. These bioactivities are influenced by species and cultivar. However, to date few studies are concerned with the effect of ripening time on fruit quality.

Metal-organic framework for the extraction and detection of pesticides from food commodities.

Pesticide residues in food matrices, threatening the survival and development of humanity, is one of the critical challenges worldwide. Metal-organic frameworks (MOFs) possess excellent properties, which include excellent adsorption capacity, tailorable shape and size, hierarchical structure, numerous surface-active sites, high specific surface areas, high chemical stabilities, and ease of modification and functionalization. These promising properties render MOFs as advantageous porous materials for the extraction and detection of pesticides in food samples.

Recent progress in mycotoxins detection based on surface-enhanced Raman spectroscopy.

Mycotoxins are toxic compounds naturally produced by certain types of fungi. The contamination of mycotoxins can occur on numerous foodstuffs, including cereals, nuts, fruits, and spices, and pose a major threat to humans and animals by causing acute and chronic toxic effects. In this regard, reliable techniques for accurate and sensitive detection of mycotoxins in agricultural products and food samples are urgently needed.

Label-free hairpin-like aptamer and EIS-based practical, biostable sensor for acetamiprid detection.

Acetamiprid (ACE) is a kind of broad-spectrum pesticide that has potential health risk to human beings. Aptamers (Ap-DNA (1)) have a great potential as analytical tools for pesticide detection. In this work, a label-free electrochemical sensing assay for ACE determination is presented by electrochemical impedance spectroscopy (EIS).

Co-occurrence of antiseptic triclocarban and chiral anti-inflammatory ibuprofen in environment: Association between biological effect in sediment and risk to human health.

Residues of antiseptics and drugs have been ubiquitously detected in aquatic water-sediment systems, and are thus considered emerging contaminants that threaten our global environment. To investigate the potential risk of ibuprofen and triclocarban in sediment, effects of enzyme activity on the enantioselective degradation in sediment were investigated. Enantioselective fate of rac-ibuprofen was observed in sediment with R-enantiomer exhibiting preferential degradation.

Hairpin DNA assisted dual-ratiometric electrochemical aptasensor with high reliability and anti-interference ability for simultaneous detection of aflatoxin B1 and ochratoxin A.

The simultaneous detection of multiple mycotoxins in grains is significant due to the enhanced toxicity induced by their synergistic effects. In this work, a dual-ratiometric electrochemical aptasensing strategy for the simultaneous detection of aflatoxin B1 (AFB1) and ochratoxin A (OTA) was developed. Here, an anthraquinone-2-carboxylic acid (AQ)-labelled complementary DNA (cDNA) was used to provide separate and specific binding sites to assemble the ferrocene-labelled AFB1 aptamer (Fc-Apt1) and methylene blue-labelled OTA aptamer (MB-Apt2).

PCDD/Fs emissions from secondary copper production synergistically controlled by fabric filters and desulfurization.

The effects of fabric filters and desulfurization systems during secondary copper smelting on polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) concentrations, emission coefficients, and profiles were studied in an oxygen-rich smelting furnace and an anode furnace. In the anode furnace, the toxic equivalent (TEQ) concentration ranges were 0.106-1.

Electrokinetic Preseparation and Molecularly Imprinted Trapping for Highly Selective SERS Detection of Charged Phthalate Plasticizers.

Nonspecific binding and weak spectral discernment are the main challenges for surface-enhanced Raman scattering (SERS) detection, especially in real sample analysis. Herein, molecularly imprinted polymer (MIP)-based core-shell AuNP@polydopamine (AuNP@PDA-MIP) nanoparticles (NPs) are designed and immobilized on an electrochemically reduced MoS-modified screen-printed electrode (SPE). This portable electrochemical-Raman interface offers the dual functions of electrokinetic preseparation (EP) and MIP trapping of charged molecules so that a reliable SERS recognition with molecular selectivity and high sensitivity can be achieved.

Veterinary antibiotics and estrogen hormones in manures from concentrated animal feedlots and their potential ecological risks.

The spread of pharmaceutically active chemicals (PACs), such as antibiotics and estrogenic hormones from animal manures can pose threats to the ecologic environment. In this work, animal manure samples were collected from 71 concentrated animal feedlots in Northern China and investigated for 24 antibiotics and 4 estrogenic hormones. Results showed that these micropollutants were ubiquitous in manures with the concentration ranges of undetectable (ND)-543,445 μg/kg (mean: 44,568 μg/kg) for antibiotics and ND-249.

Crystallization Kinetics Modulation of FASnI Films with Pre-nucleation Clusters for Efficient Lead-Free Perovskite Solar Cells.

Tin halide perovskites are rising as promising materials for lead-free perovskite solar cells (PSCs). However, the crystallization rate of tin halide perovskites is much faster than the lead-based analogs, leading to more rampant trap states and lower efficiency. Here, we disclose a key finding to modulate the crystallization kinetics of FASnI through a non-classical nucleation mechanism based on pre-nucleation clusters (PNCs).

Natural occurrence of Alternaria mycotoxins in wheat and potential of reducing associated risks using magnolol.

Background: Wheat is one of three major food crops in China. Alternaria species can cause spoilage of wheat with consequent mycotoxin accumulation. Alternariol (AOH), alternariol monomethyl ether (AME), and tenuazonic acid (TeA) are the most common and frequently studied mycotoxins.

One-step preparation of well-dispersed spindle-like FeO nanoparticles on g-CN as highly efficient photocatalysts.

Photocatalysis has attracted wide attention due to its outstanding advantages in dealing with sewage. And compounds of metal oxides and g-CN that possess Z-type heterojunctions have become the star photocatalysts in degrading pollutants. In this paper, a novel one-step method for the preparation of highly efficient photocatalyst of FeO/g-CN, using Fe (NO) and urea as raw materials, is described.

Transcriptomic Insights into the Antifungal Effects of Magnolol on the Growth and Mycotoxin Production of .

is an important phytopathogen causing fruit black rot and also producing a variety of mycotoxins, such as alternariol (AOH) and alternariol monomethyl ether (AME) as two main contaminants. This could lead to economic losses of agricultural products as well as human health risks. In this study, magnolol extracted from the traditional Chinese herb, , exhibited an obvious antifungal property and could completely suppress the mycelial growth at 100 μM.

Truncated affinity-improved aptamers for 17β-estradiol determination by AuNPs-based colorimetric aptasensor.

17β-estradiol (E2) residues could enrich in organisms via food chain and lead to harmful biological effects for human body. To ascertain the binding domain of original E2 aptamer (E00) with long-sequence (76-mer), we developed novel truncated aptamers from E00, through rationally designed truncation by intercepting a single ring or a combination of rings (containing hairpin loop, interior loop or multiloop) at different sites and retaining appropriate double helix regions. Through comparison, 15-mer E09 presented improved affinity and higher specificity, indicating the hairpin loop near to 3' end of E00 served on the binding domain to E2.

Benefit-risk assessment of dietary selenium and its associated metals intake in China (2017-2019): Is current selenium-rich agro-food safe enough?

Dietary consumption of selenium-rich agro-food is an effective way to avoid selenium deficiency diseases, however, over consumption of selenium-rich agro-food will result in potential risk of selenosis and problems with associated metals. In this study, we measured the concentrations of selenium and its associated metals in 2756 common and 4894 selenium-rich agro-food samples in 10 regions of China. We found that selenium-rich rice, flour, edible fungi and algae, meat, and tea contain higher levels of associated metals than other selenium-rich agro-food samples.

Occurrence of dioxin-like POPs in soils from urban green space in a metropolis, North China: implication to human exposure.

Urban green space is a special space for urban life and natural contact and has an important impact on human health. However, little information is available on dioxin-like persistent organic pollutants (POPs) in the soils from the specific areas. We measured the concentrations of polychlorinated naphthalenes (PCNs), polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), and polychlorinated biphenyls (PCBs) in the soils from urban green space in a metropolis, North China, and found total concentrations of PCDD/Fs, PCBs, and PCNs in the range of 11.