[Antioxidation activity and protective effection of ginger oil on DNA damage in vitro].

Objective: To study the antioxidation activity and protective effect of ginger oil on DNA damage.

Method: Chemical light assay was used to detect the oxygen radicals scavenging capacity of ginger oil. The erythrocyte oxidation damage was induced by H2O2.

[Relationship between amyloid beta-protein and oxidative stress and the protective role of pituitary adenylate cyclase activating polypeptide against oxidative stress induced damage on neuro-2a cells].

Aim: To observe the relationship between amyloid beta-protein (Abeta) and oxidative stress and the protective role of pituitary adenylate cyclase activating polypeptide (PACAP, PACAP-27) against damage induced by oxidative stress (H2O2) in neurem-2a cells.

Methods: With cultured neuro-2a cells the cell survival and apoptosis were measured by MTT assay, Hoechest33258 staining, DNA ladder and the percentage of small DNA fragment.

Results: Concentration-dependent toxicity was induced with H2O2 treatment for 24 h.

[Pituitary adenylate cyclase activating polypeptide protects neuro-2a cells from beta amyloid protein cytotoxicity by modulating intracellular calcium].

MTT analysis and intracellular calcium measurement by using confocal laser scanning microscopy were used to study the possible mechanism of protective effect of pituitary adenylate cyclase activating polypeptide 27 (PACAP27) from beta amyloid protein (Abeta)-induced neurotoxicity. The results showed that treatment with PACAP (less than 0.1 micromol/L) increased the survival and reproductive ability of neuro-2a cells and protected the neuro-2a cells from being injured by Abeta.

[Pharmacognostical studies on the roots of Euphorbia fischeriana].

Morphological and histological characters of the roots of Euphorbia fischeriana Steud., used as one of the origins of traditional Chinese medicine Langdu, were described and illustrated with line drawings. TLC analysis of the above drug was also undertaken.

[Pharmacognostical studies on the roots of Euphorbia stracheyi].

Morphological and histological characters of the roots of Euphorbia stracheyi Boiss., a traditional Chinese medicine, were described and illustrated with line drawings. TLC analysis of the above drug was also undertaken.

[Activation of PACAP receptor could protect cultured hippocampal neurons against beta amyloid peptide induced neurotoxicity].

Aim: To observe the protective role of pituitary adenylate cyclase activating polypeptide (PACAP) on hippocampal neuronal apoptosis induced by beta amyloid peptide in the culture.

Methods: Hippocampal neurons were isolated from 1d old SD rat and neuronal survival and apoptosis were measured by MTT assay and DNA ladder.

Results: 25 micromol/L Abeta could induce neuron apoptosis while co-treatment with PACAP could increase the survival of hippocampal neurons.

[Study of the pulmonary heart disease computer-aided diagnosis system based on combining neural network].

We have constructed an expert sub-system for diagnosing pulmonary heart disease with back-propagation (BP) model of typical artificial neural network and BP-Hamming. The system can obtain and represent clinical data, and it has solved the problems such as obtaining automatic knowledge, representation and self-learning. The results of testing demonstrate that the method of neural network can be regarded as an expert sub-system to make intelligent diagnosis of pulmonary heart disease.