998 results match your criteria: "Beijing Institute of Biotechnology[Affiliation]"

Background: Type 2 diabetes mellitus (T2D) is a significant health concern. Research using non-human primates, which develop T2D with similar symptoms and pancreatic changes as humans, is crucial but limited by long timelines and low success rates.

Results: We targeted capture sequenced 61 normal and 81 T2D cynomolgus monkeys using a primer panel that captured 269 potential regulatory regions potentially associated with T2D in the cynomolgus monkey genome.

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Tigecycline is one of the last-resort treatment options for infections caused by carbapenem-resistant Klebsiella pneumoniae (KP). Unfortunately, tigecycline resistance is increasingly reported and causes an unprecedented public health crisis worldwide. Although studies on tigecycline resistance are expanding, the underlying mechanisms are not fully understood.

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Aerobic glycolysis is a hallmark of cancer and is regulated by growth factors, protein kinases and transcription factors. However, it remains poorly understood how these components interact to regulate aerobic glycolysis coordinately. Here, we show that sine oculis homeobox 1 (SIX1) phosphorylation integrates growth factors (e.

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Polymer-Formulated Nerve Growth Factor Shows Effective Therapeutic Efficacy for Cerebral Microinfarcts.

Adv Mater

November 2024

Beijing Advanced Innovation Center for Soft Matter Science and Engineering, College of Life Science and Technology, College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029, China.

Article Synopsis
  • * A new treatment using a specialized polymer-formulated nerve growth factor has been developed, which can penetrate the blood-brain barrier and shows promise in reducing damage to brain cells and improving function.
  • * This research indicates a potential breakthrough in addressing cerebral microinfarcts and may be beneficial for other neurodegenerative diseases.
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Lysosomal "TRAP": a neotype modality for clearance of viruses and variants.

Nat Commun

November 2024

State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China.

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is a genus of hazardous pathogens that has caused over 30 outbreaks. However, currently approved therapies are limited in scope, as they are only effective against the Ebola virus and lack cross-protection against other orthoebolaviruses. Here, we demonstrate that a previously isolated human-derived antibody, 2G1, can recognize the glycoprotein (GP) of every orthoebolavirus species.

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Bone cancer pain remains a significant clinical challenge, often refractory to conventional treatments. The upregulation of the P2X3 receptor in the dorsal root ganglia has been implicated in the pathogenesis of bone cancer pain. This study aimed to elucidate the role of the P2X3 receptor in this context and assess the therapeutic potential of receptor silencing.

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Modular self-assembling nanoparticle vaccines, represent a cutting-edge approach in immunology with the potential to revolutionize vaccine design and efficacy. Although many innovative efficient modular self-assembling nanoparticles have been designed for vaccination, the immune activation characteristics underlying such strong protection remain poorly understood, limiting the further expansion of such nanocarrier. Here, we prepared a novel modular nanovaccine, which self-assembled via a pentamer cholera toxin B subunit (CTB) domain and an unnatural trimer domain, presenting S.

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Rationally designed multimeric nanovaccines using icosahedral DNA origami for display of SARS-CoV-2 receptor binding domain.

Nat Commun

November 2024

CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China, 11 Beiyitiao, Zhongguancun, Beijing, 100190, China.

Article Synopsis
  • The study focuses on improving nanovaccines for COVID-19 by using icosahedral DNA origami particles to closely mimic the size and shape of the SARS-CoV-2 virus.
  • By varying the arrangement and quantity of receptor binding domain (RBD) antigens on the DNA origami surface, researchers found that these modifications significantly enhance B cell activation.
  • The optimized nanovaccines not only induce stronger and longer-lasting immune responses in female mouse models but also provide broader protection against various virus mutations compared to traditional mRNA vaccines.
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Article Synopsis
  • - This clinical trial compared the safety and effectiveness of an inhaled COVID-19 vaccine (Ad5-nCoV-IH) against an intramuscular vaccine (BNT-IM) in 540 vaccinated adults in Malaysia from September 2022 to May 2023.
  • - While Ad5-nCoV-IH exhibited lower immunogenicity (with a GMC ratio of 0.22) compared to BNT-IM, it resulted in fewer adverse drug reactions (39.26% vs. 64.68%) and no serious side effects were documented.
  • - Both vaccines demonstrated similar efficacy against COVID-19 variants, but the study concluded that Ad5-nCoV-IH did not meet the non
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  • High-risk human papillomavirus (HPV) is linked to cervical, anal, and oral cancers due to its E6 and E7 proteins, which are crucial for transforming normal cells into cancerous ones, but their effects on cellular processes are still not completely understood.!
  • Researchers created recombinant viruses to express HPV16/18 E6 and E7 genes in C33A cells, using RNA sequencing to analyze the impact of these genes on host gene expression related to cervical cancer.!
  • The study found that HPV16/18 E6/E7 overexpression increased levels of several cancer-related genes, with specific correlations to pathways involved in DNA replication and tumor proliferation, suggesting a detailed relationship between these proteins and cancer progression.!
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Inhalation allergies caused by cats and dogs can lead to a range of discomforting symptoms, such as rhinitis and asthma, in humans. With the increasing popularity of and care provided to these companion animals, the allergens they produce pose a growing threat to susceptible patients' health. Allergens from cats and dogs have emerged as significant risk factors for triggering asthma and allergic rhinitis worldwide; however, there remains a lack of systematic measures aimed at assisting individuals in recognizing and preventing allergies caused by these animals.

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Palbociclib is a CDK4/6 inhibitor approved for the treatment of breast cancer by suppressing cell proliferation. However, monotherapy with palbociclib was discouraging in prostate cancer, calling for a mechanism-based effective therapy. In this study, we reported in prostate cancer that palbociclib is a potent sensitizer of ferroptosis, which is worked out by downregulating the expression of TRIB3, a gene highly expressed in prostate cancer.

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Botulinum neurotoxins (BoNTs), including serotypes A and E, are potent biotoxins known to cause human poisoning. In addition to the critical protective antigen found in the full BoNT molecule, the receptor binding domain (Hc domain), BoNTs also harbour another essential protective antigen-the light chain-translocation domain (L-HN domain). Leveraging these pivotal protective antigens, we genetically engineered a series of inactivated chimeric molecules incorporating L-HN and Hc domains of BoNT/A and E.

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Article Synopsis
  • - Diarrhea is a significant public health problem globally, with limited research on coinfections caused by multiple enteric viruses, prompting this study on a child with acute gastroenteritis infected by human adenovirus (HAdV) and human astrovirus (HAstV).
  • - Researchers used RT-PCR and whole-genome sequencing to analyze the genotypes and genetic evolution of the viruses, identifying HAdV as genotype 41 and HAstV as a recombinant strain of HAstV-2 and HAstV-4.
  • - The study found that both viruses in this coinfection are rare variants in Southern China, contributing to the understanding of genetic diversity among epidemic strains and providing insights for future vaccine and medical research.
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Author Correction: An extra-erythrocyte role of haemoglobin body in chondrocyte hypoxia adaption.

Nature

October 2024

Frontier Biotechnology Laboratory, Beijing Institute of Biotechnology, Academy of Military Medical Science; Research Unit of Cell Death Mechanism, 2021RU008, Chinese Academy of Medical Science, Beijing, China.

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Article Synopsis
  • Researchers expressed key MPXV proteins using bacteria and yeast to create nine potential MPXV subunit vaccine candidates combined with adjuvants for immunization.
  • Tests showed that these vaccine candidates elicited strong neutralizing antibody responses, particularly highlighting the A35-M1R fusion protein as a promising protective antigen for future vaccine development.
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Botulinum neurotoxin (BoNT), produced by , is the most toxic protein known, capable of causing severe paralysis and posing a significant bioterrorism threat due to its extreme lethality even in minute quantities. Despite this, there are currently no FDA-approved vaccines for widespread public use. To address this urgent need, we have developed an innovative vaccine platform by fusing the neuronal binding domain of BoNT/E (Hc/E) with core-streptavidin (CS), resulting in a stable CS-Hc/E vaccine.

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Botulinum toxin (BoNT) from Clostridium botulinum is the most toxic biotoxin known and is also an important bioterrorism agent. After poisoning, the only effective treatment is injection of antitoxin. However, neutralizing nanoantibodies are safer and more effective, representing a promising therapeutic approach.

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Hepatocellular carcinoma (HCC) is characterized by a lack of obvious clinical features in the early stages and is likely to progress to advanced HCC. Advanced HCC is a highly malignant tumor. However, there are few treatment options for advanced HCC.

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Extracellular vesicles (EVs) are considered as promising candidates for predicting patients who respond to immunotherapy. Nevertheless, simultaneous detection of multiple EVs markers still presents significant technical challenges. In this work, we developed a high-throughput microdroplet-enhanced chip (MEC) platform, which utilizes thousands of individual microchambers (∼pL) as reactors, accelerating the detection efficiency of the CRISPR/Cas systems and increasing the sensitivity by up to 100-fold (aM level).

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De novo lipogenesis (DNL), a hallmark of cancer, facilitates tumor growth and metastasis. Therapeutic drugs targeting DNL are being developed. However, how DNL is directly regulated in cancer remains largely unknown.

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