An efficient and low-cost method for the determination of selenium in selenium-enriched tea by high-performance liquid chromatography coupled with 3,3'-Diaminobenzidine derivatization.

In this study, a novel analytical method was developed and validated for the quantification of selenium in selenium-enriched tea. The various valence states of selenium present in tea were converted to Se(IV) through oxidation and reduction reactions. To chemically react with Se(IV), 3,3'-Diaminobenzidine (DAB) was employed to produce Se-DAB derivatives.

Dual-use ionization source for direct and sensitive detection of trace carbamates residue.

Directly determining trace compounds in actual sample by ambient ionization mass spectrometry (AIMS) has always been a challenge. Due to the excellent adsorption and desorption properties of nanomaterials, AIMS combined with nanomaterials can improve the adsorption and desorption properties of MS. In this work, we developed a molecularly imprinted graphene oxide coated steel sheet (MIGOCS) to be used as a solid phase extraction adsorbent.

Long-term emission characteristics of VOCs from building materials.

Long-term emission behaviors of volatile organic compounds (VOCs) from indoor buildings materials heavily depend on the value of three key parameters (initial concentration C, diffusion coefficient D, partition coefficient K) that govern emissions over time. We made the first attempt to quantitatively explore the variation of parameters through a long-lasting aging test that simulates natural indoor exposure. Over a span of 431 days, we obtained a substantial dataset consisting of ten thousand data points.

Evaluation of cosmetic efficacy of lychee seed fermentation liquid.

Background: Lychee seeds were fermented by three kinds of bacteria (Lactobacillus plantarum, Saccharomyces cerevillus and ganoderma lucidum mycelium), and two effective strains were selected by two indexes of activity content and antioxidant, so as to further verify whether lychee seeds have waste multiplication effect and can protect cells damaged by oxidation from anti-inflammatory, anti-aging and safety.

Results: The contents of polyphenols, flavonoids and proteins in the solution fermented by Ganoderma lucidum mycelium did not increase, thus affecting the antioxidant capacity of the solution was far less than that of the water extract. The active content of the other two fermentation solutions was higher than that of the water extract, and the ability of scavenging free radicals of the two solutions increased with the increase of the volume fraction.

Federated Learning for Predicting Postoperative Remission of Patients with Acromegaly: A Multicentered Study.

Background: Decentralized federated learning (DFL) may serve as a useful framework for machine learning (ML) tasks in multicentered studies, maximizing the use of clinical data without data sharing. We aim to propose the first workflow of DFL for ML tasks in multicentered studies, which can be as powerful as those using centralized data.

Methods: A DFL workflow was developed with 4 steps: registration, local computation, model update, and inspection.

Online SFE-SFC-MS/MS analysis of pyraclostrobin and chiral mefentrifluconazole residues in mango and mango juice.

This study established an on-line SFE-SFC-MS/MS method for the determination of mefentrifluconazole (MFZ) enantiomers and pyraclostrobin (PY) in mango and mango juice. Key parameters of SFC separation and SFE extraction have been optimized for high efficiency, sensitivity, and environmental friendliness. Enthalpy controlled enantioseparations of MFZ were recognized by thermodynamic analysis.

In Situ Deprotection-Free Synthesis of Silver/Graphdiyne with a High Raman Sensing Effect for Detection of Polychlorophenols and Microplastics.

Since the first synthesis of graphdiyne (GDY), it has been widely receiving a lot of attention and has great application prospects in many fields, such as energy storage, catalysis, and sensing. However, the complex deprotection treatment and long reaction time limit its mass production and applications. Here, we present a strategy for the silver-catalyzed deprotection-free rapid synthesis of GDY.

Carboxyl hybrid monolithic column in-tube solid-phase microextraction coupled with UPLC-QTRAP MS/MS for the determination of amphetamine-type stimulants.

A carboxyl functionalized organic-inorganic hybrid monolithic column (TMOS-co-CES) was applied as in-tube solid-phase microextraction (SPME) sorbent combining with ultra-performance liquid chromatography-triple quadrupole/linear ion trap mass spectrometer for separation and analyzation of seven typical amphetamine-type stimulants (ATSs), including amphetamine (AM), methamphetamine (MAM), cathinone, methcathinone, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine and 3,4-methylenedioxyethylamphetamine. The application potential of TMOS-co-CES material to ATSs was preliminarily confirmed by computational simulation by using cathinone as a representative. The influences of various SPME parameters and analytical performance were investigated systematically.

[Research Status and Safety Considerations of Animal-Derived Mesh Products].

With the development of the economy and technological progress, more and more animal-derived mesh products are being utilized in the medical field for tissue and organ repair and replacement. Owing to the complexity of their structure and production process, these animal-derived meshes still face several challenges in practical applications, such as insufficient mechanical strength, rapid degradation rates, and the detection of harmful leachable substances. Among these challenges, the production process is a key factor affecting product quality.

Development of an RPA-CRISPR/Cas12a Assay for Rapid and Sensitive Diagnosis of Plant Quarantine Fungus .

is an important phytopathogenic fungus listed by China as a harmful fungus subject to phytosanitary import control. This pathogen is a threat to a wide range of plants, particularly as the causal agent of onion pink root rot, one of the most severe diseases of onions. In order to provide rapid identification and early warning of and prevent its spread, we have developed a rapid, accurate, and visually intuitive diagnostic assay for this pathogen, by utilizing recombinase polymerase amplification (RPA), coupled with CRISPR/Cas12a cleavage and fluorescence-based detection systems or paper-based lateral flow strips.

Decoding wheat contamination through self-assembled whole-cell biosensor combined with linear and non-linear machine learning algorithms.

The contamination of mycotoxins is a serious problem around the world. It has detrimental effects on human beings and leads to tremendous economic loss. It is essential to develop a rapid and non-destructive method for contamination recognition particularly for early alarm.

Integration of transcriptomics, gut microbiota, and physiology reveals the toxic response of bensulfuron-methyl in Procambarus clarkii.

Bensulfuron-methyl (BSM) enters the environment through agricultural practices, posing a threat to the health of aquatic organisms. Currently, the toxic mechanisms of BSM on crayfish (Procambarus clarkii) have not been thoroughly investigated. In this study, crayfish were exposed to BSM solutions at concentrations of 0, 5, and 10 mg/L for 48 h.

An innovative homogeneous electrochemistry coupled with colorimetry dual-model sensing strategy for perfluorooctane sulfonate based on Cu@CuO aerogel nanozyme.

By preparing Cu@CuO aerogel as a nanozyme which exhibits prominent peroxidase-like (POD) activity, an innovative homogeneous electrochemistry (HEC) coupled with the colorimetry dual-model sensing strategy is proposed to detect perfluorooctane sulfonate (PFOS) for the first time. Cu@CuO aerogel accelerates the oxidation process of colorless o-phenylenediamine to form yellow 2,3-diaminophenazinc (DAP), and meanwhile, DAP as an electroactive substance creates a reduction peak current upon the electrochemical measurements. Interestingly, in the presence of PFOS, the POD activity of Cu@CuO aerogel is inhibited since the specific coordination between PFOS and Cu(II) can cover the active sites, resulting in the color of the sensing system becoming light and the peak current of DAP decreasing.

Spatial distribution and risk assessment of perchlorate in raw cow milk from China.

Perchlorate is a ubiquitous environmental contaminant worldwide, recognized as an emerging thyroid toxicant. This study focused on the pollution status, spatial distribution, possible sources of perchlorate in raw cow milk collected from 155 dairy farms in China, as well as the health risk of exposure to perchlorate through dairy products. The results showed that the detection rate of perchlorate in raw milk was 100% with the mean of 15.

TEAD transcription factor family emerges as a promising therapeutic target for oral squamous cell carcinoma.

The treatment of oral squamous cell carcinoma (OSCC) remains a significant difficulty, as there has been no improvement in survival rates over the past fifty years. Hence, exploration and confirmation of new dependable treatment targets and biomarkers is imperative for OSCC therapy. TEAD transcription factors are crucial for integrating and coordinating multiple signaling pathways that are essential for embryonic development, organ formation, and tissue homeostasis.

LC-MS/MS-based quantitative method and metrological traceability technology for measuring components of animal origin in beef and lamb and their products.

Establishing a quantitative detection method for adulterated meat products is crucial for ensuring food safety. However, due to the lack of metrological traceability in current quantitative methods, poor reliability and comparability always occur when measuring adulterated meat. Therefore, this study established an accurate quantitative method with metrological traceability for the quantitative analysis of pork and duck meat added to beef, lamb, and their products.

Screening of IgE-Binding Epitopes of Peach Allergenic Protein Pru p 7 Based on an Immune Microarray Assay.

Pru p 7 (also named Peamaclein) is a member of the gibberellin-regulated protein family, which is the latest foodborne allergenic protein identified in peach. In this paper, the prokaryotic expression and identification of Pru p 7 were performed, and the protein properties, structure, and homology were analyzed. In addition, a preliminary screening of B-cell linear epitopes of Pru p 7 was performed by the bioinformatics software prediction method, and three epitopes were identified using slot-blot immune microarray assay combined with an immune score matrix (P-1, AA16-21, AGYQER; P-2, AA40-46, TYGNKDE; P-3, AA52-59, DLKNSKGN).

Simultaneous determination of pesticides, mycotoxins and ferulic acid in Angelica sinensis by GC/LC-Q-TOF/MS.

In this study, a strong applicable method that could determine a total of 33 pesticides (54 compounds), 11 mycotoxins and functional components (ferulic acid) simultaneously in Angelica sinensis was developed. The compatibility of the sample pretreatment method for pesticides, mycotoxins, and functional components was improved by optimizing the acidity of extraction solvents, the sequence of ice bath and oscillation, the volumetric solution, and so on. The PRiME HLB SPE pretreatment method was chosen as the optimal one when comparing four pretreatment methods.