Preparation of magnetic resonance probes using one-pot method for detection of hepatocellular carcinoma.

Aim: To prepare the specific magnetic resonance (MR) probes for detection of hepatocellular carcinoma (HCC) using one-pot method.

Methods: The carboxylated dextran-coated nanoparticles were conjugated with anti-α-fetoprotein (anti-AFP) or anti-glypican 3 (anti-GPC3) antibodies through 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS)-mediated reaction to synthesize the probes. The physical and chemical properties of the probes were determined by transmission electron microscopy (TEM) and dynamic light scattering, and the relaxivity was compared to uncombined ultrasmall superparamagnetic iron oxide nanoparticles (USPIONs) using a 1.

Differentiating primary and extragenital metastatic mucinous ovarian tumours: an algorithm combining PAX8 with tumour size and laterality.

Aims: Our aim was to analyse the utility of the algorithm combining PAX8 with clinicopathological characteristics (tumour size, laterality and patient age) in differentiating primary ovarian mucinous tumours (POMTs) from extragenital metastatic mucinous carcinomas involving the ovary (eMOMCs).

Methods And Results: Immunohistochemical staining for PAX8 was performed on formalin fixed, paraffin embedded tissues from 47 POMTs, 18 eMOMCs and 70 extragenital primary mucinous carcinomas (ePMCs) using anti-PAX8 rabbit polyclonal antibody (pAb) and anti-PAX8 rabbit monoclonal antibody (mAb). PAX8 (pAb) positive signals were found in 3/18 eMOMCs and in 32/70 ePMCs.

Identification of differentially expressed genes in pituitary adenomas by integrating analysis of microarray data.

Pituitary adenomas, monoclonal in origin, are the most common intracranial neoplasms. Altered gene expression as well as somatic mutations is detected frequently in pituitary adenomas. The purpose of this study was to detect differentially expressed genes (DEGs) and biological processes during tumor formation of pituitary adenomas.

NaDC3 Induces Premature Cellular Senescence by Promoting Transport of Krebs Cycle Intermediates, Increasing NADH, and Exacerbating Oxidative Damage.

High-affinity sodium-dependent dicarboxylate cotransporter 3 (NaDC3) is a key metabolism-regulating membrane protein responsible for transport of Krebs cycle intermediates. NaDC3 is upregulated as organs age, but knowledge regarding the underlying mechanisms by which NaDC3 modulates mammalian aging is limited. In this study, we showed that NaDC3 overexpression accelerated cellular senescence in young human diploid cells (MRC-5 and WI-38) and primary renal tubular cells, leading to cell cycle arrest in G1 phase and increased expression of senescent biomarkers, senescence-associated β-galactosidase and p16.

Comparison of the ablation ability of nucleus pulposus after 1,064 nm Nd:YAG laser and 980 nm diode laser radiation.

Objective: To compare the ablation ability of nucleus pulposus after 1,064 nm Nd:YAG laser and 980 nm diode laser radiation.

Methods: Goat spine specimen (GSS) was radiated using Nd:YAG laser and 980 nm diode laser and then divided into five groups based on the final energy--200, 400, 600, 800 and 1,000 J groups. The ablation quality of nucleus pulposus after radiation was recorded.

[A case-control study on the duration of sleeping and cerebral infarction].

Objective: To explore the relationship between duration of sleeping and cerebral infarction.

Methods: A case-control study involved 1037 cerebral infarction patients admitted by the Second Affiliated Hospital of Harbin Medical University,December 2011-December 2012 as cases. Another 1205 adults free from cerebro-vascular diseases who had undergone physical examination in the hospital at the same period, were served as controls.

Trans-umbilical endoscopic cholecystectomy with a water-jet hybrid-knife: a pilot animal study.

Aim: To investigate the feasibility and safety of Natural orifice trans-umbilical endoscopic cholecystectomy with a water-jet hybrid-knife in a non-survival porcine model.

Methods: Pure natural orifice transluminal endoscopic surgery (NOTES) cholecystectomy was performed on three non-survival pigs, by transumbilical approach, using a water-jet hybrid-knife. Under general anesthesia, the following steps detailed the procedure: (1) incision of the umbilicus followed by the passage of a double-channel flexible endoscope through an overtube into the peritoneal cavity; (2) establishment of pneumoperitoneum; (3) abdominal exploration; (4) endoscopic cholecystectomy: dissection of the gallbladder performed using water jet equipment, ligation of the cystic artery and duct conducted using nylon loops; and (5) necropsy with macroscopic evaluation.

Efficacy of percutaneous laser disc decompression on lumbar spinal stenosis.

The objective of this study is to observe the effect of percutaneous laser disc decompression (PLDD) on lumbar spinal stenosis (LSS). Thirty-two LSS patients were treated using pulsed Nd: YAG laser, of which 21 cases (11 males and 10 females with an average age of 64 years old) were followed up for 2 years. All of the 21 patients had intermittent claudication with negative straight leg raising test results.

Enlarged laminectomy and lateral mass screw fixation for multilevel cervical degenerative myelopathy associated with kyphosis.

Background Context: Either an anterior approach or a posterior approach, which aims to decompress the spinal cord and restore the sagittal alignment, has been adopted to treat multilevel cervical degenerative myelopathy (CDM) associated with kyphosis. However, there is controversy on the optimal surgical strategy for the treatment of multilevel CDM with kyphotic deformity because of the complications of each surgical approach.

Purpose: The purpose of this study was to investigate the surgical efficacy of enlarged laminectomy (removing the inside edge of facet joints and decompressing the nerve foramina) and lateral mass screw fixation for the treatment of multilevel CDM associated with kyphosis.

Mid-term efficacy of percutaneous laser disc decompression for treatment of cervical vertigo.

Objective: To observe and analyze the mid-term efficacy of percutaneous laser disc decompression (PLDD) for the treatment of cervical vertigo.

Methods: Thirty-five patients with cervical vertigo were admitted from September 2002 to December 2006, including 14 males and 21 females, aged between 35 and 79 years with an average of 59.1 years.

Medium-term follow-up findings in imaging manifestation after percutaneous laser disc decompression.

Objective: The purpose of this study was to observe the midterm follow-up findings of the change in imaging manifestation after percutaneous laser disc decompression (PLDD), and to evaluate the safety and efficacy of this operation.

Materials And Methods: A retrospective review of the imaging manifestation of the 22 patients with cervical and lumbar vertebra disease who were treated by PLDD was analyzed.

Results: There was no significant difference in the anterior, middle, and posterior height of the intervertebral spaces of pathological discs before and after operation in the last follow-up (p>0.

Efficacy evaluation of percutaneous laser disc decompression in the treatment of lumbar disc herniation.

Objective: The purpose of this study was to evaluate the efficacy of percutaneous laser disc decompression (PLDD) in the treatment of lumbar disc herniation (LDH) and identify the relationship between PLDD efficacy and age.

Background Data: Many articles have shown that the short-term clinical outcome of PLDD was fairly good. However, the continuous follow-up results over 3 years of PLDD for LDH treatment are rarely reported, and little is known about the relationship between PLDD efficacy and age.

[Combined procedure for the treatment of ankle and foot deformities secondary to spina bifida].

Objective: To study surgical strategies for ankle and foot deformities secondary to spina bifida and treatment methods for different types of deformities.

Methods: From January 1990 and July 2009, 107 patients with ankle and foot deformities secondary to spina bifida were retrospectively analyzed. There were 44 males and 63 females, with an average age of 17.

[Limited operations and Ilizarov technique for correction of congenital clubfoot in adolescents].

Objective: To observe the effectiveness of limited operations and Ilizarov techniques for correction of congenital clubfoot (CCF) in adolescents, and to explore the new methods and new ideas for functional reconstruction of CCF.

Methods: Between September 2003 and July 2010, 25 adolescent patients (40 feet) with CCF were treated. Of the 25 patients, 14 were male (20 feet) and 11 were female (20 feet) with an age range from 12 to 25 years (mean, 15.

Time trends of contact allergy to a modified European baseline series in Beijing between 2001 and 2006.

Background: Monitoring trends of sensitivity rates in a population is useful for epidemiological surveillance. The time trends for sensitivity rates of allergens have not previously been reported in China.

Objectives: The aim of the present study was to determine the prevalence and trends of contact allergy in patients with suspected allergic contact dermatitis in Beijing, China.

[The Biomechanics Influence of unilateral laminectomy and discectomy surgery on the adjacent different grades of Disc Degeneration].

Objectives: To study the stress distribution of the adjacent different grades of disc degeneration underwenting unilateral laminectomy and discectomy surgery using non-linear finite element analysis.

Methods: Based on the lumbar CT scans, the finite element model (FEM) of lumbar spinal segment (L3-L5) was established. According to L3-L4 intervertebral disc degeneration, different grades of disc degeneration (healthy, mild, moderate and severe) models were established and unilateral laminectomy and discectomy surgery were also established.

Randomized trial of the impact of a sun safety program on volunteers in outdoor venues.

Background: A suitable sun safety educational program could help the public avoid sun exposure-induced skin damage. The objective of this study was to assess the impact of a sun safety program on volunteers in outdoor venues and explore the most effective sun safety education method in China.

Methods: An intervention program was implemented to raise knowledge and behavior regarding sun exposure among volunteers in the outdoor competition venues in Beijing, China.

Differential diagnosis between malignant and benign hepatic tumors using apparent diffusion coefficient on 1.5-T MR imaging: a meta analysis.

This meta-analysis evaluates the diagnostic accuracy of diffusion-weighted MR imaging with ADC values for differentiation between malignant and benign hepatic focal lesions. From retrieved articles, we selected 7 sets of data in 6 articles, which met all the included criteria, for meta-analysis. The pooled sensitivity and specificity were 86% (95% CI, 0.

[Nocturnal penile tumescence and the effect of tadalafil on erectile dysfunction].

Objective: To explore the correlation between the results of the nocturnal penile tumescence (NPT) test and the efficacy of tadalafil in the treatment of ED.

Methods: A total of 188 ED patients were divided into a normal NPT group (n = 136) and an abnormal NPT group (n = 52) according to the results of the NPT test. All of them were treated with tadalafil at 20 mg 3 times a week.

[Clinical analysis of 107 patients with foot and ankle deformities caused by spinal bifida].

Objective: To analyze the incidence, clinical features, deformity categories and orthopedic treatment of foot and ankle deformities caused by spinal bifida.

Methods: The charts of the patients received surgical treatment between January 1990 and July 2009 were studied retrospectively, and the data were analyzed.

Results: One hundred and seven cases of foot and ankle deformities caused by spinal bifida received surgical treatment and were included.

Genetic analysis of Brugada syndrome and congenital long-QT syndrome type 3 in the Chinese.

Background: Brugada syndrome and congenital long-QT syndrome (LQTS) type 3 (LQT3) are 2 inherited conditions of abnormal cardiac excitability characterized clinically by an increased risk of ventricular tachyarrhythmias. SCN5A gene that encodes the cardiac sodium channel α subunit is responsible for the 2 diseases, and more work is needed to improve correlations between SCN5A genotypes and associated clinical syndromes.

Methods And Results: Four patients diagnosed as having Brugada syndrome, 9 patients suspected to have Brugada syndrome, and 3 LQTS patients suspected to be LQT3 without mutations in KCNQ1 and HERG participated in the study.

[Analysis of middle-term outcome of the imaging characteristics after the percutaneous laser disc decompression].

Objective: To study the imaging findings outcome of the percutaneous laser disc decompression (PLDD) and evaluate the middle-term safety and efficacy of PLDD.

Methods: The imaging data of 22 cases suffered cervical spondylosis or lumbar spondylosis and treated by PLDD more than 3 years were retrospectively reviewed. To observe the intervertebral space of the invasive disc and the intervertebral disc hernia before and after the operation on the lateral projection of X-ray and the axial view of the MRI.

[Value of multi-slice spiral CT portal venography in prediction of esophagogastric variceal bleeding].

Objective: To assess the clinical value of multi-slice spiral CT portal venography in prediction of esophagogastric variceal bleeding.

Methods: The relation was detected between esophagogastric variceal bleeding and the location of left gastric vein orifice on portal system. Also the correlation was studied between the severity of esophageal varices endoscopically and the diameter of portal vein with multi-slice spiral CT portal venography.