Guidelines for the diagnosis and treatment of osteoarthritis in China (2019 edition).

Osteoarthritis (OA) is a degenerative disease of middle-aged and elderly people, contributed a higher burden of disease in China and the world. In 2017, under the support of the Rheumatology and Immunology Expert Committee of the Cross-Strait Medical and Health Exchange Association. The objective was to develop an evidence-based diagnosis and treatment guideline for OA in China based on emerging new evidence.

[The value of soft tissue neurogenic tumors in the extremities with 3.0 T magnetic resonance imaging].

Objective: To explore the diagnostic value of 3.0T MRI in neurogenic tumor of soft tissue in the extremities.

Methods: The MRI appearance of 17 neurogenic tumors with pathological confirmation was retrospectively analyzed.

[Experimental study on effect of Bailing Cpsule on dendritic cells in mice].

Objective: Comparing with the classic immuno-supressors, to probe in the in vitro effect of Cordyceps Sinensis (CS) on the differentiation, maturation and function of dentritic cells (DCs), and further to explore its mechanism.

Methods: Mice myeloid DCs were cultured respectively with extraction of Bailing Capsule (CSE), a Chinese medical preparation made of CS, Rapamycin and Tacrolimus, and the effect of various drugs on phenotype of DCs was analyzed with flow cytometer. Then, using as the stimulator, the DCs cultured with different drugs were mixed and cultured with heterogenous lymphocytes for observing the stimulating capacity of DCs on cell proliferation.

[A epidemiological survey and management analysis of asthma among employees of 6 chemical plants in Beijing area].

Objective: To estimate the prevalence and to evaluate the current status for management of asthma among employees in chemical plants in Beijing area.

Methods: A general survey was conducted among 12694 employees in 6 large chemical plants in Beijing area by face to face interview and physical examination.

Results: The prevalence of asthma was 1.

[Study on the risk factors of deep venous thrombosis in acute hospitalized stroke patients].

Objective: To identify the risk factors of deep venous thrombosis (DVT) in hospitalized patients with acute stroke, under a perspective case-control study.

Methods: 488 cases with stroke, identified by CT or MRI and admitted to the department of neurology and neurosurgery in Beijing Chaoyang Hospital between December 2001 and December 2002 were consecutively studied. There were 328 male and 160 female patients (95.

[Effect of propofol on the activation of nuclear factor-kappa B and expression of inflammatory cytokines in cerebral cortex during transient focal cerebral ischemia-reperfusion: experiment with rats].

Objective: To investigate the effect of propofol on the activation of nuclear factor-kappa B (NF-kappa B) and the expression of inflammatory cytokines, such as interleukin-1 (IL-1), tumor necrosis factor-alpha (TNF-alpha), and intercellular adhesion molecule-1 (ICAM-1) in cerebral cortex during transient focal cerebral ischemia-reperfusion, and to discuss the probable mechanism of its protective effect.

Methods: Ninety male Wistar rats were randomly divided into 3 equal groups: sham operation group undergoing sham operation; ischemia/reperfusion (I/R) group undergoing thread embolism of the left middle cerebral artery occlusion (MCAO) to cause focal ischemia for 2 hours and then undergoing reperfusion; and propofol group undergoing peritoneal injection of propofol 2 hours before the ischemia-reperfusion of MCAO. Then the rats in the 3 groups were re-divided into subgroups of 5 rats, totally 18 subgroups, to be decapitated 2, 3, 6, 12, 24, and 72 hours after reperfusion for the latter 2 groups, and their brains were taken out and fixed.

[Detection of deletional determinants of alpha-thalassemia in Li people in Hainan province by a single tube multiplex polymerase chain reaction method].

Objective: To develop a single tube multiplex polymerase chain reaction technique (mPCR) and use it to screen 3 deletional alpha-globin genes, namely -alpha(3.7), -alpha(4.2) (single gene deletion), and --SEA (South East Asia double gene deletion) in the Li people in Hainan province.

[The prevalence of deep venous thrombosis in hospitalized patients with stroke].

Objective: To investigate the prevalence and the clinical manifestations of DVT in hospitalized patients with acute stroke, and to evaluate the short-term prophylaxis effect of low molecular weight heparin (LMWH) to prevent DVT.

Methods: 488 cases with stroke admitted to the department of neurology and neurosurgery in Beijing Chaoyang Hospital between December 2001 and December 2002 were consecutively investigated. The diagnosis of stroke was identified by CT or MRI.

Methylation of p16 and p15 genes in multiple myeloma.

Objective: To investigate the frequency of p16 and p15 gene methylation in multiple myeloma (MM), and its relationship with bone marrow cell apoptosis and clinical outcome.

Methods: Twenty-two patients with MM were studied to detect p16 and p15 gene methylation. Methylation-specific polymerase chain reaction (MSP) was used to detect gene methylation, and terminal transferase-mediated dUTP nick end-labeling (TUNEL) was used to detect cell apoptosis.

[Gene diagnosis for hemophilia a using A combination of 4 techniques].

Objective: To improve the gene diagnosis,carrier detecting and prenatal diagnosis for hemophilia A families to a maximum.

Methods: Long distance PCR (LD-PCR) was used to detect FVIII gene inversion among 26 patients with severe HA and their female offspring to identify those with FVIII gene inversion and its carriers. Three gene linkage analysis based upon PCR, Bcl I PCR/RFLP, St14 VNTR/PCR analysis and two simple dinucleotides repeats polymorphism analysis were used among 19 non-inversion HA families for indirect diagnosis.

[A clinical analysis of 798 cases of M protein positive patients].

Objective: To improve the understanding of the diseases with positive M protein by analyzing 798 cases, so as to reduce the failure and error of diagnosis.

Methods: The relations of diseases with the types of the disease and age and sex of the patients were analyzed. The presenting symptoms of multiple myeloma(MM) and non-MM were compared.