Impact of molecular structure of starch on the glutinous taste quality of cooked chestnut kernels.

Chestnuts are a starchy food with a characteristic glutinous taste that is often used to assess their quality. In this study, our findings indicated that chestnuts with higher glutinous taste quality had lower amylose content and microcrystalline structures, as well as higher subcrystalline structures and relative crystallinity in both the raw and steamed starches. In the leached starch, chestnuts with higher glutinous taste quality had lower amylopectin B1 chains and microcrystalline structure, but higher amylopectin B2 chains, subcrystalline structure and relative crystallinity.

Whole-Transcriptome Analysis on the Leaves of under Exposure to Polycyclic Aromatic Hydrocarbons.

The leaves of plants can be recommended as a cheap and sustainable environmental protection tool to mitigate PAHs with high toxicity in the ambient environment because they can serve as a reactor to remove ambient PAHs. Although previous studies have demonstrated that PAHs exhibit toxicological features, our knowledge about how ambient PAHs influence the leaves of plants is limited regarding the leaves of plants reducing ambient PAHs as a reactor. In this study, 1-year-old .

Mixed oligosaccharides-induced changes in bacterial assembly during cucumber ( L.) growth.

The application of oligosaccharides can promote plant growth by increasing photosynthesis or inducing plant innate immunity. However, the mechanisms by which oligosaccharides affect bacterial community diversity and abundance remain unclear. In this study, a mixed oligosaccharide was applied to the growth of cucumbers.

A subpopulation of CD146 macrophages enhances antitumor immunity by activating the NLRP3 inflammasome.

As one of the main tumor-infiltrating immune cell types, tumor-associated macrophages (TAMs) determine the efficacy of immunotherapy. However, limited knowledge about their phenotypically and functionally heterogeneous nature restricts their application in tumor immunotherapy. In this study, we identified a subpopulation of CD146 TAMs that exerted antitumor activity in both human samples and animal models.

Development of a novel forensic age estimation strategy for aged blood samples by combining piRNA and miRNA markers.

In forensic investigations, age estimation is vital for determining whether a suspect is under or over the legally defined adult age. With breakthroughs in RNA sequencing technology, small noncoding RNAs have provided new ways to solve problems related to the age estimation of trace or aged samples, owing to their small molecular weight and better stability. In our previous study, we had applied miRNAs for the age estimation of bloodstains; however, further improvement of the existing model is needed.

Degradation characteristics of polybutylene adipate terephthalic acid (PBAT) and its effect on soil physicochemical properties: A comparative study with several polyethylene (PE) mulch films.

The degradation process of different types of mulch in agriculture and its effect on soil ecosystem should be considered comprehensively. To this end, the changes in performance, structure, morphology, and composition of PBAT film during the degradation process were examined through a multiscale approach in comparison with several PE films and their effects on the soil physicochemical properties were investigated. At the macroscopic scale, the load and elongation of all films decreased with increasing ages and depths.

Preparation of Titania-Silica Composite Aerogel at Atmospheric Pressure and Its Catalytic Performance in the Synthesis of Poly (Butylene Succinate).

Titanates are widely used in the synthesis of polyesters, such as Poly (butylene succinate) (PBS), due to their excellent catalytic activity for polycondensation. However, the hydrolysis sensitivity of titanate and side reactions at high temperatures restrict the further improvement of the molecular weight of polyesters and lead to the high content of end carboxyl group content in the products. In this work, we prepared titania-silica composite aerogels with resistance to hydrolysis and large specific surface area, which were further explored as an efficient catalyst for polycondensation reactions.

Mass Spectrometry-Guided Discovery of Multi--Methylated Cyclodecapeptides Auyuittuqamides E-H from sp. QL0466.

Mass spectrometry-based dereplication and prioritization led to the discovery of four multi--methylated cyclodecapeptides, auyuittuqamides E-H (-), from a soil-derived sp. The planar structures of these compounds were elucidated based on analysis of HRESIMS and NMR data. Absolute configurations of the chiral amino acid residues were assigned by a combination of the advanced Marfey's method, chiral-phase LC-MS analysis, and -based configuration analysis, revealing that - contain both d- and l-isomers of -methylleucine (MeLeu).

Investigation of Microplastics (≥10 μm) in Meconium by Fourier Transform Infrared Microspectroscopy.

Microplastics are prevalent emerging pollutants with widespread distribution in air, land and water. They have been detected in human stool, blood, lungs, and placentas. However, human fetal microplastic exposure remains largely under-studied.

Magnetization of amorphous FeOOH chrysanthemum-like nanosheets under ambient conditions.

The conversion of paramagnetic solid iron oxides to magnetic iron oxides has drawn considerable interest, but it's still challenging to carry out without heat treatment or irradiation. In this work, solid amorphous iron oxide chrysanthemum-like nanosheets synthesized by a smart redox strategy at the interface of a soap-free emulsion are magnetized at room temperature and under ambient pressure. An amorphous FeOOH hollow shell with needles towards water is produced by the reaction of cumyl hydroperoxide (CHPO) and iron(II) sulfate (FeSO) at the interface of the emulsion generated by ultrasound and torn up into chrysanthemum-like nanosheets simultaneously.

Charge-Separated Pd-Cu Atom Pairs Promote CO Reduction to C.

Positively charged Cu sites have been confirmed to significantly promote the production of multicarbon (C) products from an electrochemical CO reduction reaction (CORR). However, the positively charged Cu has difficulty in existing under a strong negative bias. In this work, we design a Pd-CuN catalyst containing charge-separated Pd-Cu atom pair that can stabilize the Cu sites.

DNA methylation-based age prediction with bloodstains using pyrosequencing and random forest regression.

The use of DNA methylation to predict chronological age has shown promising potential for obtaining additional information in forensic investigations. To date, several studies have reported age prediction models based on DNA methylation in body fluids with high DNA content. However, it is often difficult to apply these existing methods in practice due to the low amount of DNA present in stains of body fluids that are part of a trace material.

The Effects of Drug Addiction and Detoxification on the Human Oral Microbiota.

Drug addiction can powerfully and chronically damage human health. Detoxification contributes to health recovery of the body. It is well established that drug abuse is associated with poor oral health in terms of dental caries and periodontal diseases.

Automatic Multi-Plug Filtration Cleanup Tip-Filtration with Ultra-Performance Liquid Chromatography/Tandem Mass Spectrometry Detection For 22 Pesticide Residues in Typical Vegetables.

An automatic multi-plug filtration cleanup (m-PFC) tip-filtration method was developed to reduce the manual operation workload in sample preparation. In this work, m-PFC was based on multi-walled carbon nanotubes mixed with primary secondary amines and anhydrous magnesium sulfate (MgSO4) in a packed column for analysis of pesticide residues followed by ultra-performance liquid chromatography coupled with tandem mass spectrometry. Method validation was performed on 22 pesticide residues in carrot, spinach and leek, at spiked levels of 5, 10 and 50 μg/kg, respectively.

Simultaneous Cross-type Detection of Water Quality Indexes via a Smartphone-App Integrated Microfluidic Paper-Based Platform.

Water quality guarantee in remote areas necessitates the development of portable, sensitive, fast, cost-effective, and easy-to-use water quality detection methods. The current work reports on a microfluidic paper-based analytical device (μPAD) integrated with a smartphone app for the simultaneous detection of cross-type water quality parameters including pH, Cu(II), Ni(II), Fe(III), and nitrite. The shapes, baking time, amount, and ratios of reaction reagent mixtures of wax μPAD were optimized to improve the color uniformity and intensity effectively.

Effect of Cooking Method and Enzymatic Treatment on the in vitro Digestibility of Cooked and Instant Chestnut Flour.

Microwave treatment, roasting, boiling, and enzymatic treatment were used to prepare cooked and instant chestnut flour, and the in vitro digestibility were compared. Cooking gelatinized the starch and destroyed the granular and crystal structure, increasing starch digestibility. After enzymatic hydrolysis, starches were degraded by 20~24%, and the reducing sugar content of the instant flours increased by 79~94%.

Improved stability of β-carotene by encapsulation in SHMP-corn starch aerogels.

This study aimed to prepare a starch-based aerogel with microporous network structure, and to investigate its physicochemical properties after β-carotene encapsulation. Corn starch aerogels (CSA) prepared with sodium hexametaphosphate (SHMP) as a cross-linking agent and β-carotene encapsulation were evaluated in terms of morphology, long- and short-range molecular order, bioavailability, and stability. After encapsulating β-carotene, the morphology of SHMP-CSA showed that the aerogels presented agglomeration, and the relative crystallinity increased from 17.

Factors influencing the starch digestibility of starchy foods: A review.

Starchy foods are a major energy source of the human diet, their digestion is closely related to human health. Most foods require lots of processing before eating, therefore, many factors can influence starch digestibility. The factors that affect the digestibility of starches have been widely discussed previously, but the extracted starches in those studies were different from those present within the actual food matrix.

Determination of 31 Polycyclic Aromatic Hydrocarbons in Plant Leaves Using Internal Standard Method with Ultrasonic Extraction-Gas Chromatography-Mass Spectrometry.

The method for the determination of 16 priority polycyclic aromatic hydrocarbons (PAHs) in plant leaves has been studied extensively, yet the quantitativemethod for measuring non-priority PAHs in plant leaves is limited. A method for the simultaneous determination of 31 polycyclic aromatic hydrocarbons (PAHs) in plant leaves was established using an ultrasonic extraction-gas chromatography-mass spectrometry-internal standard method. The samples of plant leaves were extracted with ultrasonic extraction and purified with solid-phase extraction columns.

The tissue factor expression on CD14++CD16-monocytes is a new markers in the Chinese Han older adult population with sepsis: A prospective study.

Objective: To investigate the tissue factor (TF) expression on different subsets of monocyte and tissue factor secretion of peripheral blood and evaluate their association with the prognosis of sepsis in the Chinese older adult population.

Methods: Monocyte subsets and TF expression on different subsets of monocyte were measured using flow cytometry in 80 older adult sepsis patients and 40 age and sex matched healthy controls. Plasma level of TF was measured using ELISA (enzyme-linked immunosorbent assay) method.

Biodegradable Metal-Organic-Frameworks-Mediated Protein Delivery Enables Intracellular Cascade Biocatalysis and Pyroptosis .

Pyroptosis is a new type of regulated cell death that is of great interest for developing new strategies for treating cancers. This potential is however greatly limited by the low efficiency and selectivity of current strategies to regulate cancer cell pyroptosis. Herein, we report biodegradable metal-organic frameworks (MOFs) for intracellular delivery of glucose oxidase (GOx) that promotes cascade biocatalysis inside cells and selectively induces cancer cell pyroptosis.

A novel method for the direct detection of light stabilizer Tinuvin 622 in polymer additives by gel permeation chromatography combined with multi-angle laser light scattering.

Tinuvin 622, an oligomeric light stabilizer, is widely used in plastics to reduce light and heat induced degradation and extend their service life, therefore its detection is of great importance for quality control of plastic products. However, the classical analytical methods of Tinuvin 622, such as chromatography and mass spectrometry, are difficult to achieve direct qualitative and quantitative analysis, and simultaneously to obtain the molecular weight and molecular weight distribution information. Herein, we propose for the first time the combination of gel permeation chromatography with multi-angle laser light scattering as a simple and direct method to detect Tinuvin 622 in polymers and simultaneously to obtain its molecular weight distribution information.

Hapten designs based on aldicarb for the development of a colloidal gold immunochromatographic quantitative test strip.

The common carbamate insecticide aldicarb is considered one of the most acutely toxic pesticides. Herein, rational design was used to synthesize two haptens with spacers of different carbon chain lengths. The haptens were then used to immunize mice.

Application of Microbiome in Forensics.

Recent advances in next-generation sequencing technologies and improvements in bioinformatics have expanded the scope of microbiome analysis as a forensic tool. Microbiome research is concerned with the study of the compositional profile and diversity of microbial flora as well as the interactions between microbes, hosts, and the environment. It has opened up many new possibilities for forensic analysis.

[Determination of organophosphorus pesticide residues in milk of cows and sheep by ultra-high-performance liquid chromatography- tandem mass spectrometry combined with passing type purification method].

Objective: A method for the rapid determination of dichlorvos, trichlorfon, fenthion, fenthion-sulfone, fenthion-sulfoxide, fenthion-oxon, fenthion-oxon-sulfone, fenthion-oxon-sulfoxide, phoxim, propetamphos, malathion, diazinon and coumaphos 13 common organophosphorus pesticides and their metabolites poison residues in milk of cows and sheep by ultra-high-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) with passing type solid phase extraction(SPE) purification was developed.

Methods: After centrifugation at 4 ℃, the milk was purified by passing type SPE with acetonitrile precipitating protein and determined by UPLC-MS/MS in electrospray positive ion mode(ESI+) and multi-reaction monitoring scanning(MRM), external standard method for quantitative analysis with matrix matching standard curve.

Results: The recoveries of 13 target compounds were between 81.