[A method of exosomes extraction from large-volume cell perfusate].

To establish an efficient method for extracting exosomes from large-volume cell perfusate. EA.HY926, an immortalized cell line produced by the hybridization of human umbilical vein endothelial cells and human lung adenocarcinoma cell line A549, was cultured with M199 culture medium containing 10% fetal bovine serum.