Comparison between ZenoTOF 7600 system and QTOF for plant metabolome: an example of metabolomics applied to coffee leaves.

Introduction: ZenoTOF is new class of high-resolution mass spectrometer that combines resolution and sensitivity. This mass spectrometer is well designed to perform metabolomics.

Methods: In this context, we compared the performance of ZenoTOF 7600 system (Sciex) with QTOF6520 (Agilent Technologies) through the leaf metabolome analysis of two Coffea species, namely C.

Carving Metal-Organic-Framework Glass Based Solid-State Electrolyte Via a Top-Down Strategy for Lithium-Metal Battery.

Traditional polymer solid electrolytes (PSEs) suffer from low Li conductivity, poor kinetics and safety concerns. Here, we present a novel porous MOF glass gelled polymer electrolyte (PMG-GPE) prepared via a top-down strategy, which features a unique three-dimensional interconnected graded-aperture structure for efficient ion transport. Comprehensive analyses, including time-of-flight secondary ion mass spectrometry (TOF-SIMS), Solid-state 7Li magic-angle-spinning nuclear magnetic resonance (MAS-NMR), Molecular Dynamics (MD) simulations, and electrochemical tests, quantify the pore structures, revealing their relationship with ion conductivity that increases and then decreases as macropore proportion rises.

Unmet needs in the management of patients with bilateral synchronous renal masses: the rationale for clinical decision-making.

Background: Bilateral synchronous renal masses (BSRMs) are a rare finding, and the optimal treatment strategy remains undetermined. This study depicts the management of BSRM at eight European high-volume centers.

Methods: This is a retrospective analysis of prospective institutional databases collecting all patients presenting with clinical T1-2 N0 M0 BSRMs between 1993 and 2020 at 8 tertiary referral high-volume centers for renal cancer treatment in Europe.

Androgens differentially modulate glucocorticoid effects on adipose tissue and lean mass.

Glucocorticoids and androgens affect each other in several ways. In metabolic organs such as adipose tissue and the liver, androgens enhance glucocorticoid-induced insulin resistance and promote fat accumulation in male mice. However, the direct contribution of the androgen receptor (AR) to these effects is unknown.

Metacognitive confidence and affect - two sides of the same coin?

Decision confidence is a prototypical metacognitive representation that is thought to approximate the probability that a decision is correct. The perception of being correct has also been associated with affective valence such that being correct feels more positive and being mistaken more negative. This suggests that, similarly to confidence, affective valence reflects the probability that a decision is correct.

In silico, in vitro and in vivo characterisation of thiamin binding proteins from plant seeds.

Thiamin, an essential micronutrient, is a cofactor for enzymes involved in the central carbon metabolism and amino acids pathways. Despite efforts to enhance thiamin content in rice by incorporating thiamin biosynthetic genes, increasing thiamin content in endosperm remains challenging, possibly due to a lack of thiamin stability and/or a local sink. The introduction of storage proteins has been successful in biofortification strategies and similar efforts targeting thiamin led to a 3-4-fold increase in white rice.

A Practical Synthetic Route to Cinnolines: Application to the Design and Synthesis of RSV NNI Inhibitor JNJ-8003 Analogues.

The manuscript describes the development of an efficient synthetic route to cinnolines, facilitating faster access to JNJ-8003 related Respiratory Syncytial Virus (RSV) non-nucleoside (NNI) inhibitors. Starting from correctly functionalized aryl halides, a Sonogashira reaction followed by SNAr reaction with hydrazine 1,2-dicabroxylate reagents provided dihydrocinnolines directly via in situ 6-endo-dig cyclization. The dihydrocinnolines were conveniently transformed to corresponding cinnolines in one step.

Challenges in standardizing preimplantation kidney biopsy assessments and the potential of AI-Driven solutions.

Purpose Of Review: This review explores the variability in preimplantation kidney biopsy processing methods, emphasizing their impact on histological interpretation and allocation decisions driven by biopsy findings. With the increasing use of artificial intelligence (AI) in digital pathology, it is timely to evaluate whether these advancements can overcome current challenges and improve organ allocation amidst a growing organ shortage.

Recent Findings: Significant inconsistencies exist in biopsy methodologies, including core versus wedge sampling, frozen versus paraffin-embedded processing, and variability in pathologist expertise.

Plant species, inundation, and sediment grain size control the development of sediment stability in tidal marshes.

Tidal marshes can contribute to nature-based shoreline protection by reducing the wave load onto the shore and reducing the erosion of the sediment bed. To implement such nature-based shoreline erosion protection requires the ability to quickly restore or create highly stable and erosion-resistant tidal marshes at places where they currently do not yet occur. Therefore, we aim to identify the drivers controlling the rate by which sediment stability builds up in young pioneer marshes.

Transition to Adult Care in Turner Syndrome: Research Gaps and Strategies for Achieving Success.

Health care transition is a process by which children with chronic medical conditions gradually and purposefully move from pediatric to adult-centered health care systems. While transition guidelines have been published by multiple national and international organizations, transition processes have not been optimized for many populations, including youth with Turner syndrome (TS). Numerous barriers exist, at both the system and individual/family level.

Transferrin Saturation, Serum Iron, and Ferritin in Heart Failure: Prognostic Significance and Proteomic Associations.

Background: Iron deficiency (ID) is currently defined as a serum ferritin level <100 or 100 to 299 ng/mL with transferrin saturation (TSAT) <20%. Serum ferritin and TSAT are currently used to define absolute and functional ID. However, individual markers of iron metabolism may be more informative than current arbitrary definitions of ID.

Development of an easy non-destructive particle isolation protocol for quality control of red blood cell concentrates.

The extracellular vesicle release in red blood cell concentrates reflects progressive accumulation of storage lesions and could represent a new measure to be implemented routinely in blood centres in addition to haemolysis. Nevertheless, there is currently no standardized isolation protocol. In a previous publication, we developed a reproducible ultracentrifugation-based protocol (20,000 ×  protocol) that allows to classify red blood cell concentrates into three cohorts according to their vesiculation level.

Maleic Acid-Butanol Pretreatment to Enhance Cellulose Accessibility for Enzymatic Hydrolysis and Ethanol Production from Oil Palm Empty Fruit Bunch.

Pretreatment of lignocellulosic biomass is crucial yet challenging for sustainable energy production. This study focuses on enhancing enzymatic accessibility of cellulose in oil palm empty fruit bunches by optimizing pretreatment parameters to improve glucose and ethanol yields while reducing fermentation inhibitors. It evaluates the impact of maleic acid concentrations on biorefinery processes.

Slow-Release Pharmaceutical Implants in Ecotoxicology: Validating Functionality across Exposure Scenarios.

Pharmaceutical contaminants have spread in natural environments across the globe, endangering biodiversity, ecosystem functioning, and public health. Research on the environmental impacts of pharmaceuticals is growing rapidly, although a majority of studies are still conducted under controlled laboratory conditions. As such, there is an urgent need to understand the impacts of pharmaceutical exposures on wildlife in complex, real-world scenarios.

The interplay between mitochondrial DNA genotypes, female infertility, ovarian response, and mutagenesis in oocytes.

Study Question: Is there an association between different mitochondrial DNA (mtDNA) genotypes and female infertility or ovarian response, and is the appearance of variants in the oocytes favored by medically assisted reproduction (MAR) techniques?

Summary Answer: Ovarian response was negatively associated with global non-synonymous protein-coding homoplasmic variants but positively associated with haplogroup K; the number of oocytes retrieved in a cycle correlates with the number of heteroplasmic variants in the oocytes, principally with variants located in the hypervariable (HV) region and rRNA loci, as well as non-synonymous protein-coding variants.

What Is Known Already: Several genes have been shown to be positively associated with infertility, and there is growing concern that MAR may facilitate the transmission of these harmful variants to offspring, thereby passing on infertility. The potential role of mtDNA variants in these two perspectives remains poorly understood.

Climate change aggravated wildfire behaviour in the Iberian Peninsula in recent years.

Climate change is considered to affect wildfire spread both by increasing fuel dryness and by altering vegetation mass and structure. However, the direct effect of global warming on wildfires is hard to quantify due to the multiple non-climatic factors involved in their ignition and spread. By combining wildfire observations with the latest generation of climate models, here we show that more than half of the large wildfires (area>500 ha) occurring in the Iberian Peninsula between 2001 and 2021 present a significant increase in the rate of spread with respect to what it would have been in the pre-industrial period, attributable to global warming.

A two-wave survey study examining the impact of different sources of pregnancy information on pregnancy-related anxiety among Swedish women.

Introduction: During pregnancy, women rely on a variety of sources to obtain information. However, not all of these sources are equally reliable, and there is the concern that especially online information-seeking may increase pregnancy-related anxiety. This study examines to what extent different sources of pregnancy information are associated with concurrent pregnancy-related anxiety (RQ1) and changes in pregnancy-related anxiety throughout the pregnancy (RQ2).

Antiphospholipid antibodies positivity as a potential risk factor for restenosis following arteriovenous fistula stenting in hemodialysis patients: a pilot study.

Background: The arteriovenous fistula (AVF) is the preferred vascular access for hemodialysis. AVF stenosis is a common complication, often requiring balloon angioplasty. For recurrent stenosis, AVF stenting may be an option.

Fungal Planet description sheets: 1697-1780.

Novel species of fungi described in this study include those from various countries as follows: , from accumulated snow sediment sample. , on leaf spots of . , on submerged decaying wood in sea water, on , as endophyte from healthy leaves of .

It's Hard to Prepare for Task Novelty: Cueing the Novelty of Upcoming Tasks Does Not Facilitate Task Performance.

Rapidly learning new tasks, such as using new technology or playing a new game, is ubiquitous in our daily lives. Previous studies suggest that our brain relies on different networks for rapid task learning versus retrieving known tasks from memory, and behavioral studies have shown that novel versus practiced tasks may rely on different task configuration processes. Here, we investigated whether explicitly informing about the novelty of an incoming task would help participants prepare for different task configuration processes, such as pre-adjusting working memory gating functions.

Human stem cell models for Marfan syndrome: a .

The introduction of pluripotent stem cells into the field of disease modelling resulted in numerous opportunities to study and uncover disease mechanisms in a petri dish. This promising avenue has also been applied to model Marfan syndrome, a disease affecting multiple organ systems, including the skeletal and cardiovascular system. Marfan syndrome is caused by pathogenic variants in , the gene encoding for the extracellular matrix protein fibrillin-1 which ensembles into microfibrils.

Phase stabilization of cesium lead iodide perovskites for use in efficient optoelectronic devices.

All-inorganic lead halide perovskites (LHPs) and their use in optoelectronic devices have been widely explored because they are more thermally stable than their hybrid organic‒inorganic counterparts. However, the active perovskite phases of some inorganic LHPs are metastable at room temperature due to the critical structural tolerance factor. For example, black phase CsPbI is easily transformed back to the nonperovskite yellow phase at ambient temperature.