The Key Proteins of Dopaminergic Neurotransmission of Human Peripheral Blood Lymphocytes: Changed mRNA Level in Alcohol Dependence Syndrome.

The expression of dopamine receptor (DRD), Nurr1 transcription factor (NR4A2), and α-sinucleine (SNCA) genes in peripheral blood lymphocytes is evaluated. The results indicate that alcohol dependence is associated with high expression of SNCA and DRD4 (signifi cantly higher than in the control group) and is not associated with changes in the work of NR4A2 and DRD3 genes. The levels of DRD3 and DRD4 mRNA form a positive linear correlation (p≤0.

Participation of IIb-IIIa glycoprotein in spontaneous platelet aggregation.

In some patients with stable and unstable angina pectoris and in some donors without clinical manifestations of cardiovascular diseases and other pathologies, spontaneous platelet aggregation was completely suppressed by glycoprotein IIb-IIIa antagonists blocking the interaction of this glycoprotein with fibrinogen. Antibodies inhibiting binding of glycoprotein Ib with von Willebrand factor had no effect on the level and rate of spontaneous platelet aggregation. In the donor group, the level of spontaneous aggregation was almost 1.

[Quantitative analysis of inducible systems of Escherichia coli and Bacillus subtilis using radioimmunological methods].

By RIA dot-blot method two main cell system, system SOS-repair and heat shock system, was showed possibility to receive as well qualitative as quantitative results influence of different induced agents. Was showed quantitative differences in initial levels enzymes of RecA and CroEL in different strains of E. coli, as well of principle possibility to using received antibody, which has high specific to this enzymes, for investigation reactions this systems in the other bacterial species, such as Bac.

[Proteins induction by cysteamine in Escherichia coli cells].

It was shown, what in presence of oxygen in broad interval time of treatment in E. coli cells was induced line proteins by cysteamine which are proteins SOS--repair system and heat--shock system. There are quantitative defined induction level RecA, GroEL and DnaK proteins which are members this systems.

Rat DNA sequence associated with a complex form of DNA polymerase alpha in nonregenerating liver interacts with a ubiquitous transcription/replication factor Oct-1.

A ubiquitous mammalian transcription factor, Oct-1 (also known as OTF-1, NF-A1, OBP100, or NFIII), stimulates the initiation of replication of adenovirus DNA, and may also be involved in the activation of some chromosomal replication origins. If this is true, binding sites for Oct-1 should be present within regions responsible for the initiation of DNA replication. In this study such a binding site has been identified within a 340bp fragment that was originally isolated from a minor fraction of DNA associated with a complexed form of DNA polymerase alpha from nonregenerating rat liver, and which shows autonomous replication sequence activity in a transient transfection assay.

Effect of injection of RNA isolated from normal and epileptic cortexes of rabbits into functioning mollusc neurons.

Epileptogenic foci were formed in rabbit visual cortex by freezing with liquid nitrogen. RNA isolated from the epileptogenic cortex (RNAepl), or from the frontal lobes (RNAcont) was injected into spontaneously active neurons of the mollusc Planorbarius corneus. The amplitude and duration of the spontaneous action potentials generated following the injection of RNAepl were reproducibly higher than those produced following the introduction of RNAcont.