MALDI-TOF MS analysis for detection of bovine coronavirus with tryptic peptides from viral proteins.

Bovine coronavirus (BCoV）, a significant cattle pathogen causing enteric and respiratory diseases, is primarily detected using reverse transcription-polymerase chain reaction. Our objective was to develop a novel detection method for BCoV by matrix-assisted laser desorption/ionization‒time-of-flight mass spectrometry (MALDI-TOF MS）. Peptide mass fingerprint analysis revealed that nucleocapsid (N）, membrane (M）, and hemagglutinin-esterase (HE) were three main BCoV proteins.

Post-operative vestibular function outcomes evaluated by video Head Impulse Test in patients with non-vestibular schwannoma cerebellopontine angle tumors.

Objectives: To evaluate post-operative semicircular canal function in patients with non-vestibular schwannoma (VS) cerebellopontine angle (CPA) tumors by video Head Impulse Test (vHIT).

Methods: Fourteen patients with non-VS CPA tumors who underwent surgery. The gain in vestibulo-ocular reflex (VOR) was examined pre- and post-operatively for the semicircular canals in patients with non-VS CPA tumors.

Local Administration of (-)-Epigallocatechin-3-Gallate as a Local Anesthetic Agent Inhibits the Excitability of Rat Nociceptive Primary Sensory Neurons.

While the impact of (-)-epigallocatechin-3-gallate (EGCG) on modulating nociceptive secondary neuron activity has been documented, it is still unknown how EGCG affects the excitability of nociceptive primary neurons in vivo. The objective of the current study was to investigate whether administering EGCG locally in rats reduces the excitability of nociceptive primary trigeminal ganglion (TG) neurons in response to mechanical stimulation in vivo. In anesthetized rats, TG neuronal extracellular single unit recordings were made in response to both non-noxious and noxious mechanical stimuli.

Extracting and analyzing micronuclei from mouse two-cell embryos fertilized with freeze-dried spermatozoa.

Abnormal chromosome segregation (ACS) in preimplantation embryos causes miscarriages. For a normal pregnancy, it is necessary to reduce ACS occurrences in embryos. However, the causes of such abnormalities are unclear because no method to extract the segregated chromosomes from the blastomeres for detailed analysis.

Acanthomatous ameloblastoma with mucinous glandular differentiation in a dog.

This report describes an atypical case of canine acanthomatous ameloblastoma (CAA) with mucinous glandular differentiation in a 12-year-old male Chihuahua with a right mandibular mass. Initially diagnosed as CAA by biopsy, computed tomography revealed bone lysis, prompting surgical excision. Histological examination showed lobular growth with two distinct neoplastic cell populations: typical CAA-like cells and a minor glandular structure population.

Hypoxia-induced increase in sphingomyelin synthase 2 aggravates ischemic skeletal muscle inflammation.

Critical limb ischemia (CLI) is the most advanced stage of peripheral arterial disease, posing a high risk of mortality. Sphingomyelin, a sphingolipid synthesized by sphingomyelin synthases (SMSs) 1 and 2, plays an essential role in signal transduction as a component of lipid rafts. However, the role of sphingomyelin in the inflammation of ischemic skeletal muscles remains unclear.

Effect of feeding spent coffee grounds on the methane production in bovine rumen.

The environmental impact of methane, a greenhouse gas emitted from ruminants, is a pressing issue and methods to control methane emissions from ruminants are being investigated worldwide. In this study, we investigated the effects of the administration of spent coffee grounds (SCG) on methane production in the rumen in two cows. In the control condition (days 1 and 2), the cows were fed a basic diet twice daily (roughage and concentrate), and in the SCG condition (days 1 and 2) sequentially, the cows were fed the same basic diet and administered SCG into the rumen twice daily.

Histological effects of combined therapy involving scar resection, decellularized scaffolds, and human iPSC-NS/PCs transplantation in chronic complete spinal cord injury.

Chronic complete spinal cord injury (SCI) is difficult to treat because of scar formation and cavitary lesions. While human iPS cell-derived neural stem/progenitor cell (hNS/PC) therapy shows promise, its efficacy is limited without the structural support needed to address cavitary lesions. Our study investigated a combined approach involving surgical scar resection, decellularized extracellular matrix (dECM) hydrogel as a scaffold, and hNS/PC transplantation.

Validity of a self-administered food frequency questionnaire for genomic and omics research among pregnant women: the Tohoku Medical Megabank Project Birth and Three-Generation Cohort Study.

Background: The Tohoku Medical Megabank Project has initiated the Birth and Three-Generation Cohort Study (TMM BirThree Cohort Study) including genomic and omics investigations, and conducted a self-administered food frequency questionnaire with the response option "constitutionally unable to eat or drink it" for individual food items (TMM-FFQ) for pregnant women. This study evaluated the validity of the TMM-FFQ among pregnant women.

Methods: Participants comprised 122 pregnant women aged ≥20 years residing in Miyagi Prefecture who completed weighed food records (WFRs) for 3 days as reference intake and the TMM-FFQ during mid-pregnancy.

Oral treatment with catechin isolated from Japanese green tea significantly inhibits the growth of periodontal pathogen Porphyromonas gulae and ameliorates the gingivitis and halitosis caused by periodontal disease in cats and dogs.

It has been postulated that 90 % of cats and dogs develop periodontal disease by 1 year of age. Periodontal disease develops because of infection by multiple bacteria, including Porphyromonas gulae (P. gulae) and Porphyromonas gingivalis (P.

Development and Validation of a Novel Four Gene-Pairs Signature for Predicting Prognosis in DLBCL Patients.

Diffuse large B-cell lymphoma (DLBCL) is the most common subtype of non-Hodgkin's lymphoma. Because individual clinical outcomes of DLBCL in response to standard therapy differ widely, new treatment strategies are being investigated to improve therapeutic efficacy. In this study, we identified a novel signature for stratification of DLBCL useful for prognosis prediction and treatment selection.

Validity of a Web-Based 24-Hour Dietary Recall of Energy and Nutrient Intakes in Japanese Adults.

Recently, web-based dietary assessment tools for the targeted population have been developed and used to estimate the dietary intake level in several epidemiological studies. This study aimed to examine the validity of estimating energy and nutrient intake by the web-based 24 h dietary recall (Web24HR), which we developed for the Japanese population. Overall, 228 adults aged ≥20 years who agreed to participate were included.

Neuron-associated retroelement-derived protein Arc/Arg3.1 assists in the early stages of alphaherpesvirus infection in human neuronal cells.

Alphaherpesviruses, including herpes simplex virus type 1 (HSV-1) and pseudorabies virus (PRV), are neurotropic double-stranded DNA viruses. Alphaherpesviruses control the expression of various host factors to ensure efficient infection and propagation. Recently, HSV-1 was found to upregulate Arc/Arg3.

Visual and olfactory signals of conspecifics induce emotional contagion in mice.

Emotional contagion occurs in many animals, including rodents. To determine the social signals of emotional state-matching between individuals in mice, we conducted an empirical laboratory experiment using visual, olfactory and auditory stimuli. The Japanese wild-derived mouse strain MSM/Ms (MSM) was tested as observers, since our initial experiments indicated that MSM mice showed higher sensitivity to others' pain compared with the laboratory strain C57BL/6J (B6).

spp. as Reservoirs of Multidrug Resistance- and Biofilm-Forming in Urban Community from Southern Thailand.

Rats are rodents commonly found in Thailand that carry various zoonotic pathogens. Bacterial zoonosis can occur in a shared environment between humans and rats, especially in human communities and agricultural areas. , particularly pathogenic and multidrug-resistant strains, is a significant public health concern that is transmitted by rats.

Reprogramming canine cryopreserved hepatocytes to hepatic progenitor cells using small molecule compounds.

Introduction: Exploring techniques for differentiating and culturing canine hepatocytes serves as a means to establish systems for liver transplantation and drug metabolism testing. However, establishing consistent methods for culturing stable hepatocytes remains a challenge. Recently, several investigations have shown the reprogramming of mature hepatocytes into hepatic progenitor cells by applying specific small molecule compounds, including Y-27632, (a ROCK inhibitor), A-83-01 (a TGFβ inhibitor), and CHIR99021 (a GSK3 inhibitor) (termed YAC) in rat, mouse, and humans, respectively.

Development of Dembo-PCR for cattle in accordance with Japanese livestock quarantine guidelines.

Detection of microorganisms from bovine using real-time PCR (Dembo-PCR) is a comprehensive detection technique that was developed to detect pathogens causing bovine diseases. In Japan, the definitive tests for monitored infectious diseases, which are defined by law, are carried out at government agencies. On the other hand, the existence of pathogens other than monitored infectious diseases are not well understood.

Establishment of a novel clonal GFP-expressing transgenic ginbuna crucian carp.

The clonal triploid ginbuna crucian carp Carassius auratus langsdorfii, a naturally occurring gynogenetic fish, is suitable for cell transplantation studies to reveal the roles of stem cells and immune cells. To ensure long-term traceability of donor cells within recipient fish, we have established a transgenic ginbuna line that expresses green fluorescent protein (GFP). The Xenopus laevis ef1a promoter was introduced for regulating GFP expression.

A pilot study investigating serum carnitine profile of cats with preclinical hypertrophic cardiomyopathy.

This study aimed to prospectively investigate the hypothesis that myocardial energy metabolism changes in the early stages of feline hypertrophic cardiomyopathy (HCM) by evaluating the blood carnitine profiles. Fourteen client owned cats with HCM stage B1 and 22 clinically healthy cats were included in this study. Total-carnitine, free-carnitine, acylcarnitine, and acylcarnitine/free-carnitine ratio (AF ratio) in serum were measured in both groups.

Recurrence of Solitary Fibrous Tumor in the Spinal Cord Following Gross Total and Subtotal Resection: A Case Report of Recurrence 19 Years of Post-total Resection and Systematic Literature Review.

Spinal cord solitary fibrous tumors (SFTs), previously known as hemangiopericytoma (HPC), represent exceedingly rare neoplasms. Strategies for their management, such as appropriate follow-up duration, remain controversial due to their propensity for recurrence despite extended periods of quiescence. We report a 51-year-old male presenting with new-onset back pain and gait disturbances, who had undergone gross total resection (GTR) of an SFT within the thoracic spinal cord 19 years ago.

An estimation of lysine requirements of fattening pigs maintained and raised in Japan.

This study aimed to update the first-liming amino acid (lysine) requirements of fattening pigs maintained and raised in Japan using 146 fattening pigs with an average weight of 62 kg. Five diets were prepared with total lysine concentrations of 0.45%, 0.