Hierarchical clustering analysis of musculoskeletal stress factors and their risk level in cardboard manufacturing: research from PLIBEL.

Objective: A hierarchical cluster analysis for the cardboard manufacturing industry was developed with the aim of identifying similarities between workstations in terms of musculoskeletal stress factors (MSFs) and their impact on workers' bodies.

Methods: The hierarchical clustering analysis was conducted using data from 44 workstations, which included 6 body part divisions and 51 stress factors as variables with 36 answer options. Firstly, the content of the dataset was defined.

Climate Change and Potential Health Effects in Mexican Children.

Climate change (CC) is the most important challenge of our time, a long-term global problem and one of the most serious global threats to human health in the future. CC is the expression of changes in temperature and water cycle, floods and drought events, extreme heat waves and sea level rise. Children are particularly vulnerable because they are highly sensitive to climate changes.

Quantifying health impacts and economic costs of PM exposure in Mexican cities of the National Urban System.

Objectives: To estimate avoidable mortality, potential years of life lost and economic costs associated with particulate matter PM exposure for 2 years (2013 and 2015) in Mexico using two scenarios of reduced concentrations (i.e., mean annual PM concentration < 12 µg/m and mean annual PM concentration < 10 µg/m).

Quercetin induces G2 phase arrest and apoptosis with the activation of p53 in an E6 expression‑independent manner in HPV‑positive human cervical cancer‑derived cells.

Cervical cancer is the second most common cancer in women worldwide. Human papillomavirus (HPV) infection appears to be a necessary factor in the development of almost all cases (>95%) of cervical cancer. HPV E6 induces a change of control of p53 stabilization from Hdm2 to E6/E6AP in HPV‑infected cells.

Gallic acid sensitizes paclitaxel-resistant human ovarian carcinoma cells through an increase in reactive oxygen species and subsequent downregulation of ERK activation.

Paclitaxel (PTX) is currently used as a front-line chemotherapeutic agent for several types of cancer, including ovarian carcinoma; however, PTX-resistance frequently arises through multiple mechanisms. The development of new strategies using natural compounds and PTX in combination has been the aim of several prior studies, in order to enhance the efficacy of chemotherapy. In this study, we found the following: (i) gallic acid (GA), a phenolic compound, potentiated the capacity of PTX to decrease proliferation and to cause G2/M cycle arrest in the PTX-resistant A2780AD ovarian cancer cell line; (ii) GA exerted a pro-oxidant action by increasing the production of reactive oxygen species (ROS), and co-treatment with the antioxidant agent N‑acetyl-L‑cysteine (NAC) prevented GA+PTX-induced cell proliferation inhibition and G2/M phase arrest; (iii) PTX stimulated ERK phosphorylation/activation, and co-treatment with the MEK/ERK inhibitor PD98049 potentiated the proliferation inhibition and G2/M phase arrest; (iv) and finally, GA abrogated the PTX-induced stimulation of ERK phosphorylation, a response that was prevented by co-treatment with NAC.

Validation of Serological Antibody Profiles Against Human Papillomavirus Type 16 Antigens as Markers for Early Detection of Cervical Cancer.

Cervical cancer (CC) is the second most frequent neoplasia among women worldwide. Cancer prevention programs around the world have used the Papanicolaou (Pap) smear as the primary diagnostic test to reduce the burden of CC. Nevertheless, such programs have not been effective in developing countries, thus leading to research on alternative tests for CC screening.

A comparison between the recombinant expression and chemical synthesis of a short cysteine-rich insecticidal spider peptide.

Background: The choice between heterologous expression versus chemical synthesis for synthesizing short cysteine-rich insecticidal peptides from arthropods may impact the obtainment of yields and well-folded bioactive molecules for scientific research. Therefore, two recombinant expression systems were compared to that of chemical synthesis for producing Ba1, a cysteine-rich spider neurotoxin.

Methods: The transcription of the insecticidal neurotoxin Ba1 was obtained from a cDNA library of venom glands of the spider Brachypelma albiceps.