Evaluation of a 70 kDa Excreted/Secreted Coproantigen Immunoassay for the Detection of in Dogs.

Background: We aimed to develop a sandwich ELISA, using polyclonal antibodies against excretory/secretory (E/S) antigens specific to coproantigens present in -positive dogs.

Methods: Antibodies were produced at Biological Sciences School, Autonomous University of Nuevo León, in 2023 by immunization of rabbits with antigenic extracts from in vitro cultures of larvae. Assays were performed on 100 stool samples from pet dogs, measuring sensitivity, specificity, and cross-reactivity against other parasitic infections.

Maternal Supplementation with GG Improves Glucose Tolerance and Modulates the Intestinal Microbiota of Offspring.

Introduction: Consuming hypercaloric diets during pregnancy induces metabolic, immune, and maternal intestinal dysbiosis disorders. These conditions are transferred to the offspring through the placenta and breastfeeding, increasing susceptibility to metabolic diseases. We investigated the effect of GG supplementation on offspring maternally programmed with a hypercaloric diet.

Towards Clinical Application: Calcium Waves for In Vitro Qualitative Assessment of Propagated Primary Human Corneal Endothelial Cells.

Corneal endothelium cells (CECs) regulate corneal hydration between the leaky barrier of the corneal endothelium and the ionic pumps on the surface of CECs. As CECs do not regenerate, loss of CECs leads to poor vision and corneal blindness. Corneal transplant is the only treatment option; however, there is a severe shortage of donor corneas globally.

Malassezia Folliculitis: Pathogenesis and Diagnostic Challenges.

folliculitis (MF) is a fungal infection that often presents with pruritic follicular papules and pustules, primarily affecting the upper body. Due to its clinical similarity to bacterial folliculitis, misdiagnosis and delayed treatment are common. In this report, we present the case of a 16-year-old male who developed persistent pruritic papules on his upper back and chest, initially misdiagnosed as bacterial folliculitis.

Isolation and characterization of Salmonella enterica- and Escherichia coli-specific bacteriophages of the genus Epseptimavirus from wastewater in Minnesota.

Five lytic bacteriophages specific for Salmonella enterica and Escherichia coli were isolated from wastewater in Minnesota. These phages, designated vB_Sal_EH1, vB_Sal_EH2, vB_Sal_EH3, vB_Sal_EH4, and vB_Sal_EH7, were characterized, and their genomes were sequenced. Phylogenetic analysis showed that they grouped within the genus Epseptimavirus, with genome sizes ranging from 108,554 to 115,218 bp.

Phase Angle (PhA) Is an Easy and Complementary Tool for Assessing Nutritional Status in Ulcerative Colitis (UC) Patients: A Cross-Sectional Study.

Background: Accumulating evidence has proposed phase angle (PhA) as a marker for assessing cellular integrity and nutritional status in ulcerative colitis (UC) patients; the aim of the study was to evaluate the efficacy of PhA in assessing nutritional status in patients with UC, investigating its potential as a biomarker of disease activity.

Methods: We conducted a cross-sectional study in patients with UC and healthy controls. We determined PhA by electrical bioimpedance and categorized participants through bioelectrical impedance analysis.

Types of Injuries and the Severity of Shoulder Dysfunction Associated with Diabetes Mellitus in Patients with Functional Impairment: A Case-Control Study.

Background/objectives: Patients with diabetes have been reported to experience a higher prevalence of shoulder disorders compared to those without diabetes or with other medical conditions. However, the specific types of shoulder injuries and the extent of functional impairment associated with diabetes mellitus remain unclear. This study aimed to assess the association between diabetes and specific shoulder injuries, as well as the degree of functional impairment in affected patients.

No More High Notes: A Sequel of Spontaneous Laryngeal Rupture.

Spontaneous laryngeal rupture is an unusual event, typically caused by a sudden increase in barometric pressure and biomechanical forces within the laryngotracheal complex. Triggers often include actions such as coughing, sneezing, or violent retching. Given the delicate structures involved in this region, careful assessment is essential to ensure proper management and prevent complications.

Unmasking Tinea Incognito: Case Study, Insights Into the Pathogenesis, and Recommendations.

Tinea incognito (TI) is a dermatophyte infection that often presents atypically due to the inappropriate use of corticosteroids or other immunosuppressive treatments, complicating its diagnosis and management. This case report describes a 29-year-old American Indian (Maya) female from Yucatán, Mexico, initially diagnosed with inverse psoriasis and treated with topical corticosteroids. Over several months, her condition deteriorated, with lesions spreading and worsening, ultimately revealing TI.

Cyanoacrylate Glue as an Alternative for Peritoneal Closure.

Inguinal hernia repair is one of the most common surgical procedures. Traditionally, traumatic fixations have been used to prevent mesh migration, resulting in chronic pain in the groin. However, a non-traumatic alternative with cyanoacrylate glue is offered.

The antimicrobial potential of traditional remedies of Indigenous peoples from Canada against MRSA planktonic and biofilm bacteria in wound infection mimetic conditions.

Methicillin-resistant (MRSA) is the leading cause of wound infections, often progressing into serious invasive bloodstream infections. MRSA disproportionately affects Indigenous peoples in Canada with higher rates of skin and wound infections, an example of persistent gaps in health outcomes between Indigenous and non-Indigenous peoples precipitated by the legacy of colonialism. Conversely, Indigenous peoples have long used natural remedies for infections and other diseases; however, their knowledge was rarely considered for modern medicine.

Efficacy of neutral electrolyzed water vs. common topical antiseptics in the healing of full‑thickness burn: Preclinical trial in a mouse model.

Burn injuries impose challenges such as infection risk, pain management, fluid loss, electrolyte imbalance and psychological and emotional impact, on healthcare professionals, requiring effective treatments to enhance wound healing. The present study evaluated the efficacy superoxidized electrolyzed solution (SES), with low (SES-low) or high (SES-high) concentrations of active species, alone or in combination with a formulation in gel (G), in comparison with commonly prescribed treatments for burn injury, including nitrofurazone (NF) and silver sulfadiazine (S); normal saline was used as placebo (PI). A scald burn model was established in BALB/c mice.

Transforming Agro-Industrial Waste into Bioplastic Coating Films.

Addressing the environmental impact of agro-industrial waste, this study explores the transformation of banana, potato, and orange peels into bioplastics suitable for thin coating films. We prepared six extracts at 100 g/L, encompassing individual (banana peel, BP; orange peel, OP; and potato peel, PP) and combined [BP/OP, BP/PP, and BP/OP/PP] formulations, with yeast mold (YM) medium serving as the control. Utilizing the spin-coating method, we applied 1 mL of each sample at 1000 rpm for 1 min to create the films.

Engineering 3D Printed Gummies Loaded with Metformin for Paediatric Use.

In today's pharmaceutical landscape, there's an urgent need to develop new drug delivery systems that are appealing and effective in ensuring therapeutic adherence, particularly among paediatric patients. The advent of 3D printing in medicine is revolutionizing this space by enabling the creation of precise, customizable, and visually appealing dosage forms. In this study, we produced 250 mg metformin paediatric gummies based on the semi-solid extrusion (SSE) 3D printing technique.

Healthy Change Intervention: A Cluster RCT in Preschooler Mothers in Mexico and the United States.

Objective: To assess effects of Healthy Change intervention on maternal perception of her child's body weight (MPCW), maternal feeding style, and obesogenic home environment.

Methods: A randomized control trial was conducted, consisting of two arms: the intervention group received the Healthy Change program, and the control group received the Hygiene and Accident Prevention program. A total of 356 mother-preschool child dyads participated, 182 in the intervention group and 174 in the control group, residing in Mexico and the United States.

A New Case Report of Traboulsi Syndrome: A Literature Review and Insights Into Genotype-Phenotype Correlations.

Traboulsi syndrome is a rare genetic disorder characterized by facial dysmorphism, lens subluxation, anterior segment anomalies, and spontaneous filtering blebs. The syndrome is due to mutations in the gene, which plays a crucial role in the development and maintenance of the lens. This case report describes the clinical and genetic findings in a Mexican male with Traboulsi syndrome, highlighting the identification of a novel variant.

Meibomian Gland Dysfunction and Dropout in Diabetic Patients with Non-Proliferative Diabetic Retinopathy.

This study aims to compare meibomian gland (MG) dropout and MG dysfunction (MGD) between patients with diabetes mellitus (DM) with moderate-severe non-proliferative diabetic retinopathy (NPDR) and patients with no diabetes (NDM). This prospective, transversal, age, and gender-matched case-control study included 98 DM and 106 NDM eyes. Dry eye disease (DED) and MGD evaluations were performed, including meibography (Keratograph 5M).

Association of Methylenetetrahydrofolate Reductase () Polymorphism with Osteosarcoma in a Mexican Population.

The methylenetetrahydrofolate reductase () gene 677C➔T polymorphism is capable of altering folate metabolism and can modify certain neoplasia risk. Reports have suggested that folate can have an influence on bone development and so it is of interest to know if the 677C➔T polymorphism is associated with the malignant transformation process of this tissue. The polymorphism was determined in 55 patients with osteosarcoma and in 180 healthy individuals.