Effects of paraquat treatment on fish transaminase molecular subforms.

The effects of paraquat (PQ; 1,1'-dimethyl-4,4'-bipyridylium dichloride) treatment were investigated in carp, silver carp and wels. The serum glutamic-oxalacetic transaminase (GOT; L-aspartate: 2-oxoglutarate aminotransferase, EC 2.6.

Detection of a 2 mu derivative yeast plasmid with altered properties.

The Saccharomyces cerevisiae (Sacch. cerevisiae) strain RXII, like many others, harbours plasmid DNAs. and one category of them is homologous to the 2 mu plasmid of yeast.

Effects of polyethylene terephthalate on yeast alcohol dehydrogenase.

Yeast alcohol dehydrogenase (alcohol: NAD+ oxidoreductase, EC 1.1.1.

[A method for predicting the onset of menarche].

For the period of 1981-1984 the author collected data on the menarche age of more than 32 thousand girls aged between 10-18.5 years by the so-called status-quo method. The evaluation was carried out by means of 19 factors.

Size-dependent regeneration of Gibberella protoplasts.

Regeneration ratios of protoplasts formed from the plant pathogenic gibberella fujikuroi with a mixture of lytic enzymes were studied. The heterogeneous population of protoplasts was separated into groups differing in regeneration ratio. The frequency of regeneration was higher for large protoplasts containing an increased number of nuclei.

Immobilization of pig muscle aldolase on a silica-based support.

Pig muscle aldolase was covalently attached to a silica-based support possessing aldehyde functional groups. The activity of the immobilized enzyme was 37 U/g solid, and the specific activity calculated on a bound protein basis was 1.9 U/mg protein.

Accessory root formation on a lower medial incisor.

Accessory root formation on a lower medial incisor dating from the eighth century was observed. The accessory root consisted structurally of dentin (corresponding to the root of the tooth) covered with cement but had no radicular canal. It is therefore not a real supernumerary root.

Properties of depolarizing plateau potentials in aminopyridine-induced ictal seizure foci of cat motor cortex.

The mechanisms of generation of self-sustained depolarizing plateau potentials (DPs) were studied in intracellular recordings in aminopyridine-induced ictal seizure foci in the motor cortex of the cat. In some experiments single-electrode voltage clamp techniques were used and intracellular pressure injection of aminopyridine (Ap), phorbol esters (PhEs) and tetraethylammonium (TEA) was carried out. After several ictal episodes, DPs with bursts of action potentials or with spike inactivation developed gradually in the clonic and interictal phases, without synchronism with surface ictal seizure potentials.

The A-type potassium current: methoxybenzenes increase the rate of inactivation in snail neurons.

The effects of anisole (methoxybenzene) and veratrole (1,2-dimethoxybenzene) on the resting membrane potential (RMP), action potential (AP) and membrane potassium currents were investigated in snail neurons under current- and voltage-clamp conditions. Anisole (0.9-3 mM) scarcely influenced the AP, but it moderately depolarized the membrane and increased the neuronal excitability.

Demonstration of GABA-like immunoreactivity in myenteric plexus of frog stomach.

The GABAergic innervation of the frog stomach was studied by means of an indirect immunohistochemical method. Whole mount preparations were obtained from frog stomachs after the animals had been perfused with a mixture of picric acid, glutaraldehyde and glacial acetic acid. Samples were incubated with an antiserum specific for GABA coupled to BSA with glutaraldehyde.

Purification and characterization of Cu,Zn-superoxide dismutase from common carp liver.

1. Common carp (Cyprinus carpio L.) liver Cu,Zn-superoxide dismutase (Cu,Zn-SOD) was purified and characterized.

Consecutive diaphorase-acetylcholinesterase histochemistry in the myenteric plexus of frog stomach.

An investigation was made on the frog stomach myenteric plexus with 2 different histochemical techniques. Neuronal perikarya were stained with nicotinamide-adenine-dinucleotide-diaphorase (NADHd), while the acetyl-cholinesterase (AChE) staining showed rather the axoarchitectonic arrangement of the frog myenteric plexus. In double-labelled "whole mounts", NADHd-positive cell bodies and AChE-positive nerve processes were revealed.

The A-type potassium current: catechol-induced blockage in snail neurons.

The effects of catechol (1-12.5 mM) on membrane properties, action potential and membrane ionic currents were investigated in identified snail neurons under current- and voltage-clamp conditions. Catechol hardly influenced the resting membrane potential, or the action potential amplitude and duration, but it increased the spike voltage threshold and slightly decreased the input resistance.

A physical method for separating Saccharomyces cerevisiae cells according to their ploidy.

A centrifugation technique, using genetically marked Saccharomyces cerevisiae strains, has been developed to separate Saccharomyces cerevisiae cells of different ploidy levels from exponential phase cultures. The method involves the conversion of yeast cells to protoplasts, the separation of the protoplasts on an osmotically stabilized Nycodenz gradient, and their regeneration. This type of selection may be of importance where selectable markers are not available.

Indication for deletion of two introns in the oxi-3 gene of a respiratory-competent Saccharomyces cerevisiae strain.

Physical mapping of the mitochondrial DNA of the wild-type Saccharomyces cerevisiae strain RXII revealed that most of the restriction sites as well as the location of the apocytochrome b gene were identical in comparison with the known maps of the mitochondrial genome in other Saccharomyces cerevisiae strains. In the middle of the SalI linearized map of the RXII mitochondrial DNA, a deletion was detected which resulted in the loss of two EcoRI and one BamHI restriction sites. The corresponding region, however, exists in most other laboratory strains of Saccharomyces mapped so far.

Long-lasting potentiation of synaptic transmission requires postsynaptic modifications in the neocortex.

The mechanisms of associative long-lasting potentiation (LLP) of excitatory postsynaptic potentials (EPSPs) were studied in the motor cortex of anesthetized cats. Mono- and oligosynaptic EPSPs were evoked by stimulations of thalamic VL nucleus, pyramidal tract, callosal and somatosensory system and paired with orthodromic, antidromic or current-induced action potentials. EPSP-spike stimulus pairs with 0.

Intracellular and voltage clamp studies of capsaicin induced effects on a sensory neuron model.

The acute effects of capsaicin (CAP) were studied on membrane properties, the action potential (AP) and the membrane ionic currents in the giant serotoninergic neuron of the cerebral ganglion (MCC) in the snail of Helix pomatia L. CAP (30-300 microM) depolarized the MCC, decreased the amplitude, the rate of rise and the rate of fall of the action potential. CAP prolonged the AP-duration, increased the membrane slope resistance, decreased the hyperpolarizing afterpotential and the posttetanic hyperpolarization both in normal and Na-free media.

Methods for Analysis of the C Cistron of Temperate Phage 16-3 of RHIZOBIUM MELILOTI.

A series of clear mutants of the temperate phage 16-3 of Rhizobium meliloti were isolated that included various point and deletion mutants of the C cistron, coding for the phage repressor. It was observed that recombinant genotypes, such as c(+) and ti (temperature-sensitive allele), which form turbid plaques, can be detected quantitatively as lysogenic colonies and scored even at frequencies as low as 10(-6). Point mutations, deletions and the autonomy of intracistronic second-site mutations were characterized by this method.

A comparison of two-point, three-point and deletion mapping in the C cistron of rhizobiophage 16-3, with an explanation for the recombination pattern.

A general tendency for additivity prevailed in recombination frequencies for two-point fine-structure mapping of 14 mutants in the C cistron of Rhizobium meliloti phage 16-3, with little evidence of any marker effect. Intracistronic three-point mapping indicated that double crossovers are rare. Deletion mapping indicated that the two- and three-point mapping data gave the correct order of the mutations.