No evidence for viral sequences in five lepidic adenocarcinomas (former "BAC") by a high-throughput sequencing approach.

Background: The hypothesis of an infectious etiology of the formerly named bronchiolo-alveolar carcinoma (BAC) has raised controversy. We investigated tumor lung tissues from five patients with former BAC histology using high-throughput sequencing technologies to discover potential viruses present in this type of lung cancer. Around 180 million single reads of 100 bases were generated for each BAC sample.

Surface-enhanced Raman signal for terbium single-molecule magnets grafted on graphene.

We report the preparation and characterization of monolayer graphene decorated with functionalized single-molecule magnets (SMMs). The grafting ligands provide a homogeneous and selective deposition on graphene. The grafting is characterized by combined Raman microspectroscopy, atomic force microscopy (AFM), and electron transport measurements.

Effect of electrolyte concentration on the dynamic surface tension and dilational viscoelasticity of adsorption layers of chitosan and dodecyl chitosan.

The effect of an external salt (AcONa) on the kinetics of adsorption and structure formation inside the adsorption layers (ALs) of chitosan (Ch) and dodecyl chitosan (C12Ch) as well as on the frequency dependence of the complex dilational elasticity modulus of these layers has been studied. The complex dilational elasticity modulus of adsorption layers of polymers has been measured on the drop tensiometer (Tracker, IT Concept, France) upon applying a small sinusoidal variation of the drop area with a given frequency, omega, in the range from 10(-2) to 0.63 rad/s and recording the variation of the surface pressure.

On the design of capacitive sensors using flexible electrodes for multipurpose measurements.

This article evaluates the potential of capacitive measurements using flexible electrodes to access various physical quantities. These electrodes are made of a thin metallic film, typical thickness 0.2 microm, evaporated on a plastic substrate.

NMR study of cation, anion, and solvent mobilities in macroporous poly(vinylidene fluoride).

The mobilities of lithium, PF6- and solvents in the electrolyte LiPF6-(ethylene carbonate-dimethyl carbonate-diethyl carbonate) were measured using the pulsed gradient spin-echo NMR. They were compared to those of the same electrolyte filling a macroporous poly(vinylidene fluoride) membrane. The conductivity decrease resulting in the incorporation of this macroporous membrane and the cationic transport number were analyzed in terms of (i) solvent/polymer and solvent/salt interactions, (ii) ionic dissociation, and (iii) tortuosity.

Thermal conductivity of spin-polarized liquid 3He.

We present the first measurements of the thermal conductivity of spin-polarized normal liquid 3He. Using the rapid melting technique to produce nuclear polarizations up to 0.7, and a vibrating wire both as a heater and a thermometer, we show that, unlike the viscosity, the conductivity increases much less than predicted for s-wave scattering.

Probing "cosmological" defects in superfluid 3He-B with a vibrating-wire resonator.

We report on the observation of an anomalously high damping measured by a vibrating-wire resonator (VWR) immersed into superfluid at ultralow temperatures. The observed dissipation is orders of magnitude above that corresponding to friction with the dilute normal fraction and superfluid vortices. A clear pinning behavior is also observed, as well as a strong magnetic field dependence.

Synthesis of 3-deaza-3-nitro-2'-deoxyadenosine.

Photoactivable deoxyadenosine mimic, 3-deaza-3-nitro-2'-deoxyadenosine (2), was prepared using two different synthetic routes. The first route involved base catalyzed glycosylation of 3-deaza-3-nitroadenine, which was prepared by regioselective nitration of 3-deazaadenine. In the second route, the convertible nucleoside 6-O-(2,4,6-trimethylphenyl)-3-deaza-2'-deoxyadenosine (28) was used to introduce 6-NH2 group in the last step.

Biotin-phenyldiazomethane conjugates as labeling reagents at phosphate in mono and polynucleotides.

Molecules 2-5 that include in their structure a biotin moiety as detectable unit and differently substituted phenyl diazo functions as reactive group were prepared as reagents for labeling the phosphate group in mono and polynucleotides. These molecules were shown to react selectively and quantitatively with the model nucleotide 3'-UMP. They were used successfully in the labeling step of DNA and RNA analysis using high-density DNA-chips (or microarrays) technology.

Coherent oscillations in a superconducting multilevel quantum system.

We have observed coherent oscillations in a multilevel quantum system, formed by a current-biased dc SQUID. These oscillations have been induced by applying resonant microwave pulses of flux. Quantum measurement is performed by a nanosecond flux pulse that projects the final state onto one of two different voltage states of the dc SQUID, which can be read out.

Conformational study of a guaiacyl beta-O-4 lignin model compound by NMR. Examination of intramolecular hydrogen bonding interactions and conformational flexibility in solution.

Intramolecular H-bonding interactions were investigated in solution for the threo and erythro diastereomeric forms of a guaiacyl beta-O-4 lignin model compound by using the NMR data obtained from hydroxyl protons. Temperature coefficients of the chemical shifts (ddelta/dT) and coupling constants (3J(HCOH)) were measured in aprotic and protic solutions: DMSO-d6, acetone-d6 and acetone-d6-water. The NMR parameters do not support the existence of strong and persistent intramolecular H-bonds that could participate in the stabilization of the guaiacyl beta-O-4 structure in solution, but instead indicate that intermolecular H-bonds to solvent predominate.

Bogoliubov shadow bands in the normal state of superconducting systems with strong pair fluctuations.

On the basis of a scenario where electron pairing is induced by resonant two-particle scattering (the boson-fermion model), we show how precursors of the superconducting state-in the form of overdamped Bogoliubov modes-emerge in the normal state upon approaching the transition temperature from above. This result is obtained by a renormalization technique based on continuous unitary transformations (the flow equations), projecting out the coherent contributions in the electron spectral function from an incoherent background.

Evidence of two-dimensional macroscopic quantum tunneling of a current-biased dc SQUID.

The escape probability out of the superconducting state of a hysteretic dc SQUID has been measured at different values of the applied magnetic flux. At low temperatures, the escape current and the width of the probability distribution are temperature independent but they depend on flux. Experimental results do not fit the usual one-dimensional macroscopic quantum tunneling (MQT) law but are perfectly accounted for by the two-dimensional MQT behavior as we propose here.

Aryldiazomethane derivatives as reagents for site specific labeling of nucleic acids at phosphate.

An efficient and direct labeling method based on direct alkylation of nucleic acids at phosphates by aryldiazomethane derivatives is described.

Deoxyribonolactone lesion in DNA: synthesis of fluorinated analogues.

Mono- and difluorinated derivatives of 2-deoxyribonolactone were synthesized using diastereoselective Reformatski reaction as a key step.

One-shot quantum measurement using a hysteretic dc SQUID.

We propose a single shot quantum measurement to determine the state of a Josephson charge quantum bit (qubit). The qubit is a Cooper pair box and the measuring device is a two junction superconducting quantum interference device (dc SQUID). This coupled system exhibits a close analogy with a Rydberg atom in a high Q cavity, except that in the present device we benefit from the additional feature of escape from the supercurrent state by macroscopic quantum tunneling, which provides the final readout.

High performance liquid chromatography and photodiode array detection of ferulic acid in Rubus protoplasts elicited by O-glycans from Fusarium sp. M7-1.

So far only little data have been available concerning the eliciting capacity of well defined glycan molecules isolated from plant pathogens. This study brings new information about changes in plant cells caused by fungal pathogens. Sugar fractions derived from glycoproteins isolated from the fungus Fusarium sp.

Calculating X-ray absorption near-edge structure at very low energy.

The measurement and calculation of X-ray absorption near-edge structure at very low energy can provide important information about a metallic center in biological compounds. A rapid overview of the biological applications of this technique is given, then a new method of calculating the spectra is presented. This technique, based on the use of the finite-difference method to solve the Schrödinger equation, is especially precise and potentially applicable to metalloproteins.

Chemistry of the 2-deoxyribonolactone lesion in oligonucleotides: cleavage kinetics and products analysis.

Deoxyribonolactone in DNA is an oxidized abasic site damage that is produced by a variety of physical and chemical agents such as gamma-irradiation and ene-diyne antibiotics. The extent and biological significance of the lesion are poorly documented due to the high lability of the damaged DNA. The chemistry of degradation of deoxyribonolactone-containing DNA was investigated using oligonucleotides of different length (5-, 11-, 23-, 34-mers) in which the lactone was photochemically generated, as already reported, from oligonucleotide precursors containing a photoactive nitroindole residue.

Dense suspensions and supercooled liquids: dynamic similarities.

Dense suspensions and supercooled, glass-forming, fragile Lennard-Jones liquids near T(g) should display similar relaxational and rheological behavior based on dynamical heterogeneities (actually, clusters of correlated, mobile particles embedded among the ordinary, bulk particles). Near structural arrest, we expect the shear-thinning onset to vanish as the inverse bulk-beta relaxation time. Connection to mechanical properties of granular media near random loose packing is briefly considered.

Synthesis and properties of spiro nucleosides containing the barbituric acid moiety.

The two chiral spiro nucleosides 4 and 5 containing the barbituric acid moiety were efficiently synthesized from optically pure precursors, and their properties were studied. The carbocyclic nucleoside 5 is considerably more stable against ring opening than the deoxyribosyl derivative 4. Both compounds present enhanced hydrogen bonding capacity with diacetyladenosine.

NMR and parity nonconservation. Experimental requirements to observe a difference between enantiomer signals.

The possibility of observing a difference in the high-resolution NMR spectra of two enantiomers as due to parity nonconservation is discussed. Proposals to minimize the NMR linewidths are presented. It is concluded that, using the ultra- high-resolution technique, a difference could be observed with high Z value spin one- half nuclei such as Pt, T1, Xe.

Coherent low-energy charge transport in a diffusive S-N-S junction.

We have studied the current-voltage characteristics of diffusive mesoscopic Nb-Cu-Nb Josephson junctions with highly transparent Nb-Cu interfaces. We consider the low-voltage and high-temperature regime eV < epsilon(c) < k(B)T, where epsilon(c) is the Thouless energy. The observed excess current as well as the observed subharmonic Shapiro steps under microwave irradiation suggest the occurrence of low-energy coherent multiple Andreev reflection.