RTB Lectin: a novel receptor-independent delivery system for lysosomal enzyme replacement therapies.

Enzyme replacement therapies have revolutionized patient treatment for multiple rare lysosomal storage diseases but show limited effectiveness for addressing pathologies in "hard-to-treat" organs and tissues including brain and bone. Here we investigate the plant lectin RTB as a novel carrier for human lysosomal enzymes. RTB enters mammalian cells by multiple mechanisms including both adsorptive-mediated and receptor-mediated endocytosis, and thus provides access to a broader array of organs and cells.

Applying high-throughput phenotyping to plant-insect interactions: picturing more resistant crops.

Through automated image collection and analysis, high-throughput phenotyping (HTP) systems non-destructively quantify a diversity of traits in large plant populations. Some platforms collect data in greenhouses or growth chambers while others are field-based. Platforms also vary in the number and type of sensors, including visible, fluorescence, infrared, hyperspectral, and three-dimensional cameras that can detect traits within and beyond the visible spectrum.