Rat liver endoplasmic reticulum protein kinases.

1. Rat liver microsomal membranes were studied for the presence of protein kinases. Microsomal proteins solubilized with Triton X-100 were analyzed by means of ion exchange chromatography.

"Hanseniaspora uvarum" the ultrastructural morphology of a rare ascomycete, isolated from oral thrush.

Superficial fungal infections, including oral thrush, often affect aged full denture wearers and many individuals over 65 years old. The aim of this study was to examine the ultrastructural morphology of a very rare yeast, named Hanseniaspora uvarum/guillermondi, member of the Ascomycetes family, whose pathogenesis and behaviour is not widely known. The yeast was isolated from whitish lesions of the buccal mucosa of an 70 years old woman.

Ethanol production from nonsterilized carob pod extract by free and immobilized Saccharomyces cerevisiae cells using fed-batch culture.

The production of ethanol from carob pod extract by free and immobilized Saccharomyces cerevisiae cells in batch and fed-batch culture was investigated. Fed-batch culture proved to be a better fermentation system for the production of ethanol than batch culture. In fed-batch culture, both free and immobilized S.

Gallbladder Findings after Cholecystectomy in Morbidly Obese Patients.

Morbidly obese patients constitute a high risk group for the development of gallbladder disease. In our series 70 consecutive patients underwent vertical gastroplast in an effort to manage morbid obesity. The mean age was 37 years (range 20-60), and the mean excess body weight was 92 kg (range 52-265).

Evaluation of two gonadotropin-releasing hormone (GnRH) analogues (leuprolide and buserelin) in short and long protocols for assisted reproduction techniques.

Purpose: The administration of two GnRH analogues (buserelin and leuprolide acetate) in long and short protocols was evaluated prospectively. In the long protocol, the analogues were given from day 1 of the cycle for at least 14 days and then hMG administration was started, while in the short protocol the analogues were initiated from cycle day 1, adding hMG from day 3. The patients were divided into four groups according to the protocol used: Group I, buserelin-long; Group II, buserelin-short; Group III, leuprolide-long; and Group IV, leuprolide-short.

Barley beta-glucosidase: expression during seed germination and maturation and partial amino acid sequences.

Unlike most of the hydrolytic enzymes that participate in endosperm mobilization, beta-glucosidase of barley (Hordeum vulgare) seeds does not increase during germination, even in the presence of exogenously added gibberellic acid. However, the germination process affects the physical properties of beta-glucosidase in terms of charge and apparent molecular weight. Analysis of developing barley grains shows that the enzyme is synthesized two weeks before maturation and is stored in the endosperm of the dry dormant seed.

The toxicity of organophosphate compounds towards cultured PC12 cells.

The effects of three representative organophosphates (OPs), tricresyl phosphate (TCP), triphenyl phosphite (TPP) and paraoxon (POX) on the proliferation and viability of rat PC12 pheochromocytoma cells were studied. With respect to its IC50, TCP was at least an order of magnitude more potent in its antiproliferative activity than both TPP and POX. All test OPs were cytotoxic at concentrations inhibiting cell proliferation.

A possible role for autoantibodies in left ventricular hypertrophy.

The purpose of the study was to investigate the possible role of autoantibodies in the development and type of left-ventricular hypertrophy (LVH). Three groups of subjects were studied: (a) 15 patients with hypertrophic cardiomyopathy (HCM; 11 males, 4 females; mean age 50.0 +/- 16.

Correlation of dementia, neuropsychological and MRI findings in multiple sclerosis.

Twenty patients diagnosed as having multiple sclerosis (MS) were examined by MRI and 9 neuropsychological scales: MMSE, BCRS, RMB, SDMT, BNT, VM, FAS, Benton and Hamilton. The number and distribution of the lesions, and cerebral and corpus callosum atrophy were evaluated by MRI. MR images were generated by a 0.

Kinetics of ethanol production from carob pods extract by immobilized Saccharomyces cerevisiae cells.

Kinetics of ethanol production from carob pods extract by immobilized S. cerevisiae cells in static and shake flask fermentation have been investigated. Shake flask fermentation proved to be a better fermentation system for the production of ethanol than static fermentation.

The beta-tubulin genes of Drosophila auraria are arranged in a cluster.

When the beta 1-, beta 2- and beta 3-tubulin-specific DNAs from Drosophila melanogaster were used as probes to recognize tubulin-specific sequences in the chromosomes of Drosophila auraria, they were found to hybridize to the same polytene band in region 32C of the 2L polytene chromosome. Three overlapping clones were isolated from a lambda EMBL3 genomic library of D. auraria, and they all contain beta-tubulin-specific sequences based on hybridization and partial-sequencing experiments of subcloned fragments.

Acute renal failure in a medical setting: changing patterns and prognostic factors.

The clinical characteristics of 118 patients (60 male) with acute renal failure (ARF) admitted between 1980 and 1991, were retrospectively analyzed and compared with our earlier series of the 1960s. The mean age was 53 years (16-82 years). There was a marked decline in the hypotension-related cases (43% vs.

Two forms of L-asparaginase in Tetrahymena thermophila.

In T. thermophila two forms of L-asparaginase (EC 3.5.

Relationship between interstitial infiltrates and steroid responsiveness of proteinuria in membranous nephropathy.

Mononuclear inflammatory cells were retrospectively analysed using monoclonal antibodies in the interstitium and glomeruli of 16 renal biopsy specimens from patients with nephrotic syndrome due to idiopathic membranous nephropathy (IMN). The aim of the study was to determine the composition of the infiltrate and to assess the ability to predict the response of proteinuria to corticosteroids. All patients had received prednisolone as a sole treatment.

The relationship between the sympathetic nervous system and the renin-angiotensin system in renovascular hypertension.

Unlabelled: The aim of this study is to evaluate and compare the activity of the sympathetic nervous system and the renin-angiotensin system in patients with unilateral or bilateral renovascular hypertension. Plasma noradrenaline (plNA) levels and plasma renin activity (PRA) were measured simultaneously in blood samples obtained both from a peripheral vein and from the renal veins, during catheterization of the venous cava system, in 17 patients with unilateral (group A) and in 9 patients with bilateral renal artery stenoses (group B).

Results: peripheral plNA levels were elevated in 6/17 patients of the group A and 3/9 patients of the group B, while in the remaining 11/17 and 6/9 were normal, respectively.

The diagnostic value of the determination of troponin T in the diagnosis of unstable angina.

Unlabelled: The purpose of the study is the predictive value of determination of Tr-T in diagnosis of unstable angina.

Methods: 35 pts (24 male, 11 female, mean age 53.4 +/- 5 years) were studied.

Negative symptoms of schizophrenia and the dexamethasone suppression test.

The dexamethasone suppression test (DST) was administered to 30 inpatients who met the DSM-III-R criteria for chronic schizophrenia and shared similar environments. Four of them (13%) were DST nonsuppressors. The mean and maximum postdexamethasone cortisol levels were correlated with the patient's score on the scale for the Schedule for the Assessment of Negative Symptoms and with the score on the anergia subscale of the Brief Psychiatric Rating Scale.

Toxicity study of the main alkaloids of Datura ferox in broilers.

Seeds of the weed Datura ferox are frequent contaminants of raw materials used for animal feed. These seeds produce various toxic effects and contain mainly the alkaloids scopolamine and hyoscyamine. In this 3-month toxicity study, a mixture of scopolamine and hyoscyamine (98:2) was incorporated at four total alkaloid levels (1.

Polymorphism of salivary esterase and alpha-amylase in the Greek population.

The genetic polymorphism of two salivary enzymes (esterase and alpha-amylase) was studied in individuals from eight districts of Greece. The pooled gene frequencies were: SetS = 0.63, SetF = 0.

Effect of follicular fluid on the kinetics of human sperm acrosome reaction in vitro.

The purpose of the present study was to evaluate the kinetics of human sperm acrosome reaction in vitro using the triple stain technique. Acrosome reaction was studied in sperm samples from 16 fertile men 2, 6, and 9 h after ejaculation, following incubation in culture medium (CM; Ham's F-10), with a mixture of CM and follicular fluid (FF), or with FF only. Incubation of sperm samples without the influence of any medium served as control.

Management of pseudophakic retinal detachment with various intraocular lens types.

In the last five years, 25 cases of retinal detachment (RD) after intraocular lens implantation were treated in the University Eye Clinic of Thessaloniki. The incidence of RD was lower with posterior chamber intraocular lens (PC-IOL, 0.46%) implantation than in the cases with anterior chamber IOL (AC-IOL, 2.

Growth hormone, oestradiol, progesterone and testosterone concentrations in follicular fluid after ovarian stimulation with various regimes for assisted reproduction.

Follicular fluid samples and oocytes were obtained from 75 women (87 cycles), who participated in an assisted conception programme. Determinations of the concentration of oestradiol, progesterone, testosterone and growth hormone were performed in all follicular fluid samples. Patients were stimulated with the following regimes: group A (24 cycles, 94 samples), human menopausal gonadotrophin (HMG) (three ampoules/day) and human chorionic gonadotrophin (HCG); group B (23 cycles, 53 samples), HMG/HCG with prednisolone (7.

Determination of chloramphenicol residues in eggs by high performance liquid chromatography (HPLC).

A rapid and sensitive HPLC method for the determination of chloramphenicol (CAP) residues in albumin and yolk of eggs is described. Conditions of isolation and identification are presented in detail. The detection limit is 10 ppb and the linearity is very good up to 2500 ppb CAP.